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update inputs
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acesnik committed Jun 9, 2021
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6 changes: 3 additions & 3 deletions README.md
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Expand Up @@ -7,7 +7,7 @@ The code is listed in order of execution, e.g. "1_", "2_" etc. The output of eac

The logic for these analyses is contained in the `SingleCellProteogenomics` folder.

The input files are contained in the "input" folder. This folder is linked [here](https://drive.google.com/file/d/1G4i115FCH8XNyiEHCkBXMSO_9pwGflTq/view?usp=sharing) as a zip file, `input.zip`. Expand this folder within the base directory of this repository. If you are looking for the raw imaging proteomic dataset produced after filtering artifacts and such, that is located [here](https://drive.google.com/file/d/11vjsZV-nmzPpFmA7ShbfHzmbrk057b1V/view?usp=sharing).
The input files are contained in the "input" folder. This folder is linked [here for release v1.2](https://drive.google.com/file/d/149ICTtieYjuKWZoLwRLzimwff0n6eWqw/view?usp=sharing) as a zip file, `input.zip`. Expand this folder within the base directory of this repository. If you are looking for the raw imaging proteomic dataset produced after filtering artifacts and such, that is located [here](https://drive.google.com/file/d/11vjsZV-nmzPpFmA7ShbfHzmbrk057b1V/view?usp=sharing).

The output files are added to a folder "output" during the analysis, and figures are added to a folder "figures."

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The single-cell RNA-Seq data is available at GEO SRA under project number [GSE146773](https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE146773).

The cell cycle phase and FACS intensity information for these ~1,000 cells are contained in the [input folder](https://drive.google.com/file/d/1G4i115FCH8XNyiEHCkBXMSO_9pwGflTq/view?usp=sharing) within three files, one per plate, starting with `RNAData/180911_Fucci_single cell seq_ss2-18-*.csv`.
The cell cycle phase and FACS intensity information for these ~1,000 cells are contained in the [input folder](https://drive.google.com/file/d/149ICTtieYjuKWZoLwRLzimwff0n6eWqw/view?usp=sharing) within three files, one per plate, starting with `RNAData/180911_Fucci_single cell seq_ss2-18-*.csv`.

The `snakemake` workflow used to analyze the scRNA-Seq dataset, including RNA velocity calculations and louvain unsupervised clustering, can be found in this repository: https://github.com/CellProfiling/FucciSingleCellSeqPipeline.

The `loom` file containing the results of RNA velocity analysis, including spliced and unspliced counts, can be found in the [input folder](https://drive.google.com/file/d/1mdQbYcDPqiTOHeiYbv_4RtrxrmlhYMNl/view?usp=sharing) under `RNAData/a.loom`, and the observation names used for each cell that match the "Well_Plate" identifiers can be found in `RNAData/a.obs_names.csv`.
The `loom` file containing the results of RNA velocity analysis, including spliced and unspliced counts, can be found in the [input folder](https://drive.google.com/file/d/149ICTtieYjuKWZoLwRLzimwff0n6eWqw/view?usp=sharing) under `RNAData/a.loom`, and the observation names used for each cell that match the "Well_Plate" identifiers can be found in `RNAData/a.obs_names.csv`.

## Citation

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2 changes: 1 addition & 1 deletion workflow/Snakefile
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import os

INPUTID="1G4i115FCH8XNyiEHCkBXMSO_9pwGflTq"
INPUTID="149ICTtieYjuKWZoLwRLzimwff0n6eWqw"

# scripts currently expect base directory as working directory
if os.path.basename(os.path.abspath(os.curdir)) == "workflow":
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