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Cloufield committed Jun 7, 2024
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6 changes: 5 additions & 1 deletion 05_PCA/index.html
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Expand Up @@ -2331,6 +2331,10 @@ <h1 id="principle-component-analysis-pca">Principle component analysis (PCA)</h1
<li><a href="#pca-umap">PCA-UMAP</a></li>
<li><a href="#references">References</a></li>
</ul>
<div class="admonition info">
<p class="admonition-title">PCA workflow</p>
<p><img width="600" alt="image" src="https://github.com/Cloufield/GWASTutorial/assets/40289485/6a5880c7-10bd-4fac-a364-12ab14171f72"></p>
</div>
<h2 id="preparation">Preparation</h2>
<h3 id="exclude-snps-in-high-ld-or-hla-regions">Exclude SNPs in high-LD or HLA regions</h3>
<p>For PCA, we first exclude SNPs in high-LD or HLA regions from the genotype data. </p>
Expand Down Expand Up @@ -2375,7 +2379,7 @@ <h3 id="download-bed-like-files-for-high-ld-or-hla-regions">Download BED-like fi
</code></pre></div>
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<h3 id="create-a-list-of-snps-in-high-ld-or-hla-regions">Create a list of SNPs in high-LD or HLA regions</h3>
<p>Next, use <code>high-ld.txt</code> to extract all SNPs which are located in the regions described in the file using the code as follows:</p>
<p>Next, use <code>high-ld.txt</code> to extract all SNPs that are located in the regions described in the file using the code as follows:</p>
<div class="highlight"><pre><span></span><code>plink --file ${plinkFile} --make-set high-ld.txt --write-set --out hild
</code></pre></div>
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2 changes: 1 addition & 1 deletion search/search_index.json

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