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Got exit code 1 (indicating failure) from job _toil_worker cgp file:/Comparative-Annotation-Toolkit/Darmor_A01_qwe/toil/augustus_cgp/jobStore kind-cgp/instance-cf_rpblx. #307

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jwli-code opened this issue Jan 8, 2024 · 1 comment

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@jwli-code
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When I ran CAT, the following error occurred. The following is the input file and code I provided. I don't know what caused this error.
I only provide hal file and second generation transcriptome data here, gff is only the result of one chromosome, and transcriptome data is the transcriptome data of all autosomes.I hope someone will help me.

[ANNOTATION] ZS11 = test_data/ZS11.v0.A01.change.gff3 [BAM] ZS11 = /home/jwli/data4/prediction/CAT/Comparative-Annotation-Toolkit/test_data/bams/ZS11/252_20DAF.srt.bam,/home/jwli/data4/prediction/CAT/Comparative-Annotation-Toolkit/test_data/bams/ZS11/454_40DAF.srt.bam,/home/jwli/data4/prediction/CAT/Comparative-Annotation-Toolkit/test_data/bams/ZS11/B4_3.srt.bam,/home/jwli/data4/prediction/CAT/Comparative-Annotation-Toolkit/test_data/bams/ZS11/SRR6453768.srt.bam,/home/jwli/data4/prediction/CAT/Comparative-Annotation-Toolkit/test_data/bams/ZS11/SRR6453769.srt.bam

luigi --module cat RunCat --hal=test_data/Brassica_napus15-pg.full.hal --ref-genome=Darmorv4.1 --workers=10 --config=test_data/Darmor_A01.config --work-dir Darmor_A01 --out-dir Darmor_A01 --local-scheduler --augustus --augustus-species=arabidopsis --augustus-cgp --binary-mode local --assembly-hub > log_darmor_a01.txt

@jwli-code
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This is my complete error message.

ERROR: 2023-12-15 19:25:27,281 - Got exit code 1 (indicating failure) from job _toil_worker cgp file:/Comparative-Annotation-Toolkit/Darmor_A01_qwe/toil/augustus_cgp/jobStore kind-cgp/instance-cf_rpblx.
WARNING: 2023-12-15 19:25:27,281 - Job failed with exit value 1: 'cgp' kind-cgp/instance-cf_rpblx
WARNING: 2023-12-15 19:25:27,302 - The job seems to have left a log file, indicating failure: 'cgp' kind-cgp/instance-cf_rpblx
WARNING: 2023-12-15 19:25:27,303 - Log from job kind-cgp/instance-cf_rpblx follows:
=========>
        [2023-12-15T19:23:30+0000] [MainThread] [I] [toil.worker] ---TOIL WORKER OUTPUT LOG---
        [2023-12-15T19:23:30+0000] [MainThread] [I] [toil] Running Toil version 4.2.0-3aa1da130141039cb357efe36d7df9b9f6ae9b5b on host e8d60324a6c4.
        [2023-12-15T19:23:30+0000] [MainThread] [I] [luigi-interface] Loaded ['/etc/luigi/luigi.cfg', 'luigi.cfg']
        [2023-12-15T19:25:10+0000] [MainThread] [E] [root] Error talking to caching database: database is locked
        [2023-12-15T19:25:10+0000] [MainThread] [I] [toil.lib.retry] Got database is locked, trying again in 0s.
        Syntax error
        augustus: ERROR
                the parsing of /tmp/node-5a821e47-0f29-43d4-9510-0990e020e818-1686a2e7-cb89-40a7-973b-a04a5d9b82be/tmpy_pptd2k/07778579-10f8-4ed5-9f57-9d43f53e52ec/tmpfpkgrhi5.tmp has been unsuccessful. Please check, whether the syntax of your input file is correct

        Traceback (most recent call last):
          File "/usr/local/lib/python3.8/dist-packages/toil/worker.py", line 368, in workerScript
            job._runner(jobGraph=jobGraph, jobStore=jobStore, fileStore=fileStore, defer=defer)
          File "/usr/local/lib/python3.8/dist-packages/toil/job.py", line 1424, in _runner
            returnValues = self._run(jobGraph, fileStore)
          File "/usr/local/lib/python3.8/dist-packages/toil/job.py", line 1361, in _run
            return self.run(fileStore)
          File "/usr/local/lib/python3.8/dist-packages/toil/job.py", line 1565, in run
            rValue = userFunction(*((self,) + tuple(self._args)), **self._kwargs)
          File "/usr/local/lib/python3.8/dist-packages/cat-2.0-py3.8.egg/cat/augustus_cgp.py", line 291, in cgp
            tools.procOps.run_proc(cmd, stdout=stdout)
          File "/usr/local/lib/python3.8/dist-packages/cat-2.0-py3.8.egg/tools/procOps.py", line 74, in run_proc
            pl.wait()
          File "/usr/local/lib/python3.8/dist-packages/cat-2.0-py3.8.egg/tools/pipeline.py", line 1157, in wait
            self.raiseIfExcept()
          File "/usr/local/lib/python3.8/dist-packages/cat-2.0-py3.8.egg/tools/pipeline.py", line 1115, in raiseIfExcept
            p.raiseIfExcept()
          File "/usr/local/lib/python3.8/dist-packages/cat-2.0-py3.8.egg/tools/pipeline.py", line 775, in raiseIfExcept
            raise self.exceptInfo[0].with_traceback(self.exceptInfo[2])
        tools.pipeline.ProcException: process exited 1: augustus --dbhints=1 --allow_hinted_splicesites=atac --extrinsicCfgFile=/tmp/node-5a821e47-0f29-43d4-9510-0990e020e818-1686a2e7-cb89-40a7-973b-a04a5d9b82be/tmpy_pptd2k/07778579-10f8-4ed5-9f57-9d43f53e52ec/tmpxjb26nc9.tmp --species=arabidopsis --treefile=/tmp/node-5a821e47-0f29-43d4-9510-0990e020e818-1686a2e7-cb89-40a7-973b-a04a5d9b82be/tmpy_pptd2k/07778579-10f8-4ed5-9f57-9d43f53e52ec/tmpfpkgrhi5.tmp --alnfile=/tmp/node-5a821e47-0f29-43d4-9510-0990e020e818-1686a2e7-cb89-40a7-973b-a04a5d9b82be/tmpy_pptd2k/07778579-10f8-4ed5-9f57-9d43f53e52ec/tmpvg67zprn.tmp --dbaccess=/tmp/node-5a821e47-0f29-43d4-9510-0990e020e818-1686a2e7-cb89-40a7-973b-a04a5d9b82be/tmpy_pptd2k/07778579-10f8-4ed5-9f57-9d43f53e52ec/tmp594gimeh.tmp --speciesfilenames=/tmp/node-5a821e47-0f29-43d4-9510-0990e020e818-1686a2e7-cb89-40a7-973b-a04a5d9b82be/tmpy_pptd2k/07778579-10f8-4ed5-9f57-9d43f53e52ec/e8d60324a6c4.155185.3206550652.tmp --softmasking=1 --exoncands=1 --alternatives-from-evidence=0 --/CompPred/logreg=on --printOEs=1 --/CompPred/outdir=/tmp/node-5a821e47-0f29-43d4-9510-0990e020e818-1686a2e7-cb89-40a7-973b-a04a5d9b82be/tmpy_pptd2k/07778579-10f8-4ed5-9f57-9d43f53e52ec --printSampled=true

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