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Lilly-May committed Mar 6, 2024
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4 changes: 2 additions & 2 deletions docs/yaml_docs/pipeline_ingestion_yml.md
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Expand Up @@ -415,10 +415,10 @@ This can help to determine any inconsistencies in staining per channel and other

### Centered log ratio (CLR) normalization options

<span class="parameter">clr_margin</span> `Integer`, Default: 0<br>
<span class="parameter">clr_margin</span> `Integer`, Default: 1<br>
Margin determines whether to normalize per cell (as you would do for RNA normalization),
or by feature (recommended, due to the variable nature of prot assays).
CLR margin 0 is recommended for informative QC plots in this pipeline.
CLR margin 1 is recommended for informative QC plots in this pipeline.
- 0 = normalise row-wise (per cell)
- 1 = normalise column-wise (per feature, recommended)

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4 changes: 2 additions & 2 deletions panpipes/panpipes/pipeline_ingest/pipeline.yml
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Expand Up @@ -175,10 +175,10 @@ normalisation_methods: clr

# margin determines whether you normalise per cell (as you would for RNA),
# or by feature (recommended, due to the variable nature of prot assays).
# CLR margin 0 is recommended for informative qc plots in this pipeline
# CLR margin 1 is recommended for informative qc plots in this pipeline
# 0 = normalise row-wise (per cell)
# 1 = normalise column-wise (per feature, recommended)
clr_margin: 0
clr_margin: 1

#--------------------------------------------------------------
# Denoised and Scaled by Background (DSB) normalization options
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