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Preprocess pipeline yaml documentation #200

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merged 11 commits into from
Mar 6, 2024
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Lilly-May
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This PR introduces a separate documentation file for all the preprocess pipeline.yml parameters. Additionally, I removed all the parameter explanations from the pipeline.yml itself to make the file easier to read.

Once this PR is merged, meaning we have settled on the final format of the file, I'll also adjust the prefilled file for the preprocessing tutorial to match the structure here.

There were a few things I wasn't entirely sure about, and I'll mention those in separate comments below.


- <span class="parameter">n_cells_by_counts</span> `Integer`<br>

### Protein-specific filtering (prot)
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Should we still list the entire structure (obs: min / max, var: min / max) here, although there are no specific parameters in the respective sections?

- <span class="parameter">color_by</span> `String`, Default: sample_id<br>
Specify the covariate you want to use to color the dimensionality reduction plot.

- <span class="parameter">dim_remove</span> `TODO`<br>
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I'm not sure in what format the dim_remove parameter should be provided. As a boolean? Or an integer?

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it's an integer, it should be the component associated with tech noise (the first normally)

@Lilly-May Lilly-May requested a review from bio-la February 27, 2024 17:27
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can you add the .md to the index so it's rendered in the documentation and i can check how the filtering dictionary is rendered (and answer your comment)?
thanks!

@@ -328,8 +329,8 @@ Whether applying scaling or not is still a matter of debate, as stated in the [L
Parameter for CLR normalisation.
The CLR margin determines whether you normalise per cell (as you would normalise RNA data), or by feature (recommended, due to the variable nature of protein assays).
Hence, CLR margin 0 is recommended for informative qc plots in this pipeline.
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margin =1 is recommended

@bio-la bio-la merged commit 1f0161f into main Mar 6, 2024
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@Lilly-May Lilly-May deleted the preprocess_pipeline_yaml_doc branch April 17, 2024 08:29
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2 participants