Dose_response_workflow_biochemical

Dose response curve (DRC) workflow

Introduction

This workflow can be used to fit dose-response curves from normalised biochemical assay data (%Inhibition) using the HCS extension. This workflow needs R-Server to run in the back-end. IC₅₀ values will not be extrapolated outside the tested concentration range For activity classification the following criteria are applied:

• maximum (average % inhibion) >25 % and slope is >0 and IC₅₀ > 5 µM or
• minimum (average % inhibion) >75 % Results are formatted for upload to the European Chemical Biology Database

Data file

The KNIME workflow starts with reading a KNIME binary file (*.table). An exemplary table is contained in the workflow.

In principle, modification of the Table Reader node to a CSV Reader or Excel Reader node in the workflow can alter the input file to a CSV or and Excel sheet respectively.