Introduction Introduction Experimental Design Experimental Design Data Acquisition Introduction File Transfer wget globus connect irods/iplant/cyverse SRAtoolKit SRAtoolKit (Downloading Raw Data) Data wrangling Introduction FASTA(Q) manipulations What is a Quality Score? Determine Sequence Lengths Converting FASTQ to FASTA Extract sequences based on sequence ID Microsoft Excel Tips and Tricks Create workbook from multiple text files Create text files from workbook with multiple worksheets Create Index for all worksheets Data Analysis RNA-Seq [Data Visualization] [Appendix]