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How to create a pair of primers to amplify a plasmid? #8

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JinyuanSun opened this issue Jul 8, 2024 · 0 comments
Open

How to create a pair of primers to amplify a plasmid? #8

JinyuanSun opened this issue Jul 8, 2024 · 0 comments

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@JinyuanSun
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Hello,

I am looking for guidance on how to design a pair of primers to amplify a specific plasmid sequence. I need to ensure that one primer extends downstream (forward primer) and the other extends upstream (reverse primer) from a specified starting position. The primers do not need to be of the same length. Can I achieve this using primers?
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