ReadMe.rtf

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\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\outlinelevel0\adjustright\rin0\lin0\itap0\pararsid12129 \rtlch\fcs1 \af31507\afs24\alang1033 \ltrch\fcs0 \f31506\fs24\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 {\rtlch\fcs1 \af31507 
\ltrch\fcs0 \b\f0\ul\insrsid13641415\charrsid15095093 Calcium buffering models explanatory note:
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid13641415\charrsid15095093 
\par 
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\outlinelevel0\adjustright\rin0\lin0\itap0\pararsid12129 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \b\f0\insrsid13641415\charrsid15095093 Model Published in:
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid13641415\charrsid15095093 
\par Anwa}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7606648 r H, Hong S, De Schutter E (201}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid4211956 0}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid13641415\charrsid15095093 ) Controlling Ca}{\rtlch\fcs1 \af31507 
\ltrch\fcs0 \f0\super\insrsid13641415\charrsid15095093 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid13641415\charrsid15095093 -activated K}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid13641415\charrsid15095093 +}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid13641415\charrsid15095093  channels with models of Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid13641415\charrsid15095093 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid13641415\charrsid15095093  buffering in Purkinje cell.}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7606648  }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid7606648\charrsid7606648 Cerebellum}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid4868440  *}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid7606648  }{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7606648 
\par 
\par }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid4868440 * }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid7606648\charrsid4868440 Available as Open Access
\par }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid7606648 
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\outlinelevel0\adjustright\rin0\lin0\itap0\pararsid12129 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \b\f0\insrsid7606648\charrsid4868440 PubMed link}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid7606648 : }{\field\fldedit{\*\fldinst {\rtlch\fcs1 \af31507 \ltrch\fcs0 \insrsid9448945 HYPERLINK "http://www.ncbi.nlm.nih.gov/pubmed/20981513"}}{\fldrslt {\rtlch\fcs1 \af31507 \ltrch\fcs0 \cs16\f0\ul\cf17\insrsid7606648\charrsid12670074 
http://www.ncbi.nlm.nih.gov/pubmed/20981513}}}\sectd \ltrsect\linex0\endnhere\sectdefaultcl\sectrsid1070915\sftnbj {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7606648 
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid6890987 
\par Email contact: }{\field{\*\fldinst {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid6890987  HYPERLINK "mailto:anwar@oist.jp" }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid6890987\charrsid12670074 {\*\datafield 
00d0c9ea79f9bace118c8200aa004ba90b02000000170000000e00000061006e0077006100720040006f006900730074002e006a0070000000e0c9ea79f9bace118c8200aa004ba90b2a0000006d00610069006c0074006f003a0061006e0077006100720040006f006900730074002e006a0070000000}}}{\fldrslt {
\rtlch\fcs1 \af31507 \ltrch\fcs0 \cs16\f0\ul\cf17\insrsid6890987\charrsid12670074 anwar@oist.jp}}}\sectd \ltrsect\linex0\endnhere\sectdefaultcl\sectrsid1070915\sftnbj {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid6890987 
\par 
\par }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid13641415\charrsid15095093 
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\outlinelevel0\adjustright\rin0\lin0\itap0\pararsid12129 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \b\f0\insrsid4004595\charrsid15095093 Model description:
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid4004595\charrsid15095093 
\par In this work, we }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid10253584\charrsid15095093 compare the dynamics of different buffering models during generation of a dendritic Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid10253584\charrsid15095093 2+
}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid10253584\charrsid15095093  spike in a single compartment model of a Purkinje cell dendrite.}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid4004595\charrsid15095093  }{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid10253584\charrsid15095093  The Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid10253584\charrsid15095093 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid10253584\charrsid15095093  buffering models used are 1) a single Ca}{\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\super\insrsid10253584\charrsid15095093 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid10253584\charrsid15095093  pool, 2) two Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid10253584\charrsid15095093 2+}{\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid10253584\charrsid15095093  pools respectively }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid14558486\charrsid15095093 f}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid10253584\charrsid15095093 
or the fast and slow transients, 3) a detailed calcium model with buffers, pump}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562  (Schmidt et al., 2003)}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid5726795 ,}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid10253584\charrsid15095093  and diffusion and 4) a calcium model with buffers, pump and diffusion compensation. The parameters of }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7756216\charrsid15095093 single pool and double pool are}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid10253584\charrsid15095093  tuned, using Neurofitter}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid6320231  }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid6320231\charrsid15095093 (Van Geit }{\rtlch\fcs1 \af31507 
\ltrch\fcs0 \f0\insrsid16392562 et al.,}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid6320231\charrsid15095093  2007)}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid10253584\charrsid15095093 , to approximate the behavior of detailed calcium dynami}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid5706046\charrsid15095093 cs over range of 0.5 \u181\'b5M to 8 \u181\'b5}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid10253584\charrsid15095093 M}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid14558486\charrsid15095093  of intracellular calcium}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7756216\charrsid15095093 . The diffusion compensation is}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid14558486\charrsid15095093  modele}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8533820\charrsid15095093 d using a buffer}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid12129 -}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8533820\charrsid15095093 like mechanism}{\rtlch\fcs1 \af31507 
\ltrch\fcs0 \f0\insrsid8869077  called }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8533820\charrsid15095093 DCM. }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7756216\charrsid15095093 To use DCM robustly for different diameter compartments, its}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8533820\charrsid15095093  parameters}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid11172777\charrsid15095093  }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7756216\charrsid15095093 are}{\rtlch\fcs1 \af31507 
\ltrch\fcs0 \f0\insrsid8533820\charrsid15095093  estimated}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid6320231 ,}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7756216\charrsid15095093  using Neurofitter}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid14039451\charrsid15095093  (Van Geit }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 et al.,}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid14039451\charrsid15095093  2007)}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid6320231 ,}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid11172777\charrsid15095093  }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8533820\charrsid15095093 as a fu}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7756216\charrsid15095093 nction of com}{\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid5706046\charrsid15095093 partment diameter  (0.8 \u181\'b5m-20 \u181\'b5}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7756216\charrsid15095093 m)}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8533820\charrsid15095093 .}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7756216\charrsid15095093 
\par }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8533820\charrsid15095093  }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid14558486\charrsid15095093 
\par 
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\outlinelevel0\adjustright\rin0\lin0\itap0\pararsid12129 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \b\f0\insrsid1070915\charrsid15095093 Scripts Explanation:
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1070915\charrsid15095093 
\par Go to the folder containing the model scripts. Run nrnivmodl. Then type nrngui CaBufferingModels.hoc
\par 
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\outlinelevel0\adjustright\rin0\lin0\itap0\pararsid12129 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 A panel with six}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid1070915\charrsid15095093  buttons will appear on the screen. 
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1070915\charrsid15095093 
\par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af31507 \ltrch\fcs0 \i\insrsid15824208\charrsid15095093 \hich\af0\dbch\af31506\loch\f0 1.\tab}}\pard\plain \ltrpar
\s15\ql \fi-360\li720\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\ls1\adjustright\rin0\lin720\itap0\pararsid1070915\contextualspace \rtlch\fcs1 \af31507\afs24\alang1033 \ltrch\fcs0 \f31506\fs24\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 {
\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid15824208\charrsid15095093 Detailed Calcium }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1070915\charrsid15095093 dynamics model}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1070915\charrsid15095093 
: Click on this button to see the behavior of }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid9718770\charrsid15095093 detailed c}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16207401\charrsid15095093 
alcium dynamics model. It will display nine plots arranged in three rows and three columns. }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid11275082\charrsid15095093 The plot on the top left corner shows }{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid9718770\charrsid15095093 c}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1070915\charrsid15095093 alcium transients}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid11275082\charrsid15095093  with }{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid5706046\charrsid15095093 peak amplitude of 0.5 \u181\'b5}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid11490050 M, 1 }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid11490050\charrsid15095093 \u181\'b5}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid5706046\charrsid15095093 M and 2 \u181\'b5}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1070915\charrsid15095093 M}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid11275082\charrsid15095093 
. These transients were generated using a voltage step command (as shown in Figure 1a}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid9718770\charrsid15095093  in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{\rtlch\fcs1 \af31507 
\ltrch\fcs0 \f0\insrsid8922863 {\*\bkmkstart _GoBack}{\*\bkmkend _GoBack}2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid11275082\charrsid15095093 ) with different maximal condu}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid9718770\charrsid15095093 
ctance values of P-type }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid4868440 Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid4868440\charrsid4868440 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid11275082\charrsid15095093  channel. }{\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid15824208\charrsid15095093 All the other plots show corresponding behavior of buffers and pump current.}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid590647\charrsid15095093  These plots are same as Figur}{\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid14771632\charrsid15095093 e 2 in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid590647\charrsid15095093 .}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid4004595\charrsid15095093  }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid15824208\charrsid15095093 
\par }\pard \ltrpar\s15\ql \li720\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin720\itap0\pararsid15824208\contextualspace {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1070915\charrsid15095093  
\par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af31507 \ltrch\fcs0 \i\insrsid1070915\charrsid15095093 \hich\af0\dbch\af31506\loch\f0 2.\tab}}\pard \ltrpar
\s15\ql \fi-360\li720\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\ls1\adjustright\rin0\lin720\itap0\pararsid15824208\contextualspace {\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1070915\charrsid15095093 
Calcium transients using different buffering models}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid15824208\charrsid15095093 : Click on this button to see the calcium trans}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid5706046\charrsid15095093 
ients with peak amplitude of 2 \u181\'b5}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid15824208\charrsid15095093 M, generated using single pool, double pool, detailed calcium dynamics model and DCM. The parameters of single pool}{\rtlch\fcs1 \af31507 
\ltrch\fcs0 \f0\insrsid590647\charrsid15095093 , double pool and DCM were fitted using Neurofitter}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid14039451\charrsid15095093  (Van Geit }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 et al.,}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid14039451\charrsid15095093  2007)}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid9718770\charrsid15095093  as described in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid590647\charrsid15095093 .}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid15824208\charrsid15095093  }{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid5059091\charrsid15095093 This }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid6176863\charrsid15095093 plot is si}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid5530802 m}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid4868963 
ilar to Figure 3c and Figure 6 }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid4868963\charrsid15095093 in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid4868963 Anwar et al., 2010 }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid5530802 except that the Ca}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid5530802\charrsid5468400 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid5530802  transient in these scri}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid5468400 pts are shown for diameter 4 }{\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid5468400\charrsid15095093 \u181\'b5}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid4868963 m}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid6176863\charrsid15095093 . }{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid15824208\charrsid15095093 
\par }\pard \ltrpar\s15\ql \li720\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin720\itap0\pararsid15824208\contextualspace {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1070915\charrsid15095093 
\par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af31507 \ltrch\fcs0 \i\insrsid1070915\charrsid15095093 \hich\af0\dbch\af31506\loch\f0 3.\tab}}\pard \ltrpar
\s15\ql \fi-360\li720\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\ls1\adjustright\rin0\lin720\itap0\pararsid5059091\contextualspace {\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1070915\charrsid15095093 Calcium spikes using }{\rtlch\fcs1 \af31507 
\ltrch\fcs0 \i\f0\insrsid1988182 single pool}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1070915\charrsid15095093  model}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid590647\charrsid15095093 : }{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid4605507\charrsid15095093 Click on this button to see the calcium spikes }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid14771632\charrsid15095093 generated using single pool}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 
 model. The maximal conductance values for P-type and T-type Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid1988182\charrsid6910273 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  channels and BK-type and SK-type Ca}{\rtlch\fcs1 \af31507 
\ltrch\fcs0 \f0\super\insrsid1988182\charrsid6910273 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  activated K}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid1988182\charrsid6910273 +}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 
 channels were tuned using Neurofitter (Van Geit }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 et al.,}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  2007) and are presented in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 
Anwar et al., }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 . Note that the parameter values used for single pool in this plot are different than those use
d in plot showing calcium transients. The parameter values of single pool used to generate calcium spikes in this plot are obtained using experimental trace (shown in Figure 1b in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 ) as a voltage command. This plot }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16517658 exists}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid1988182  in Figure 7}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16517658 d}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 .
\par }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0\pararsid1988182 \rtlch\fcs1 \af31507\afs24\alang1033 \ltrch\fcs0 \f31506\fs24\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 {\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid1988182\charrsid1988182 
\par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af31507 \ltrch\fcs0 \i\insrsid1988182\charrsid15095093 \hich\af0\dbch\af31506\loch\f0 4.\tab}}\pard\plain \ltrpar
\s15\ql \fi-360\li720\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\ls1\adjustright\rin0\lin720\itap0\pararsid5059091\contextualspace \rtlch\fcs1 \af31507\afs24\alang1033 \ltrch\fcs0 \f31506\fs24\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 {
\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1988182\charrsid15095093 Calcium spikes using }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1988182 double pool}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1988182\charrsid15095093  model}{\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid1988182\charrsid15095093 : Click on this button to see the calcium spikes }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 generated using double}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182\charrsid15095093 
 pool}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  model. The maximal conductance values for P-type and T-type Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid1988182\charrsid6910273 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 
 channels and BK-type and SK-type Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid1988182\charrsid6910273 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  activated K}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\super\insrsid1988182\charrsid6910273 +}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  channels were tuned using Neurofitter (Van Geit }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 et al.,}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid1988182  2007) and are presented in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 
. Note that the parameter values used for double pool in this plot are different than those used in plot showing calcium transients. The parameter values 
of double pool used to generate calcium spikes in this plot are obtained using experimental trace (shown in Figure 1b in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8922863 2010}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 ) as a voltage command. This plot }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7606648 exists}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16517658  in Figure 7d}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid1988182  in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 .
\par }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0\pararsid1988182 \rtlch\fcs1 \af31507\afs24\alang1033 \ltrch\fcs0 \f31506\fs24\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 {\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid1988182\charrsid1988182 
\par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af31507 \ltrch\fcs0 \i\insrsid1988182\charrsid15095093 \hich\af0\dbch\af31506\loch\f0 5.\tab}}\pard\plain \ltrpar
\s15\ql \fi-360\li720\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\ls1\adjustright\rin0\lin720\itap0\pararsid5059091\contextualspace \rtlch\fcs1 \af31507\afs24\alang1033 \ltrch\fcs0 \f31506\fs24\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 {
\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1988182\charrsid15095093 Calcium spikes using }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1988182 detailed}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1988182\charrsid15095093  model}{\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid1988182\charrsid15095093 : Click on this button to see the calcium spikes }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 generated using detailed model. The maximal conductance values for P-type and T-type Ca}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid1988182\charrsid6910273 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  channels and BK-type and SK-type Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid1988182\charrsid6910273 2+}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  activated K}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid1988182\charrsid6910273 +}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  channels were tuned using Neurofitter (Van Geit }{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 et al.,}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  2007) and are presented in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 . This plot }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7606648 exists}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16517658  in Figure 7c}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid1988182  in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 .
\par }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0\pararsid1988182 \rtlch\fcs1 \af31507\afs24\alang1033 \ltrch\fcs0 \f31506\fs24\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 {\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid1988182\charrsid1988182 
\par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af31507 \ltrch\fcs0 \i\insrsid1988182\charrsid15095093 \hich\af0\dbch\af31506\loch\f0 6.\tab}}\pard\plain \ltrpar
\s15\ql \fi-360\li720\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\ls1\adjustright\rin0\lin720\itap0\pararsid1988182\contextualspace \rtlch\fcs1 \af31507\afs24\alang1033 \ltrch\fcs0 \f31506\fs24\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 {
\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1988182\charrsid15095093 Calcium spikes using }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1988182 DCM}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182\charrsid15095093 
: Click on this button to see the calcium spikes }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 generated using DCM. The maximal conductance values for P-type and T-type Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid1988182\charrsid6910273 
2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  channels and BK-type and SK-type Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid1988182\charrsid6910273 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  activated K}{\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\super\insrsid1988182\charrsid6910273 +}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  channels were tuned using Neurofitter (Van Geit }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 et al.,}{\rtlch\fcs1 \af31507 
\ltrch\fcs0 \f0\insrsid1988182  2007) and are presented in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 . This plot }{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7606648 exists}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16517658  in Figure 7c}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 .
\par }\pard\plain \ltrpar\ql \li360\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin360\itap0\pararsid1988182 \rtlch\fcs1 \af31507\afs24\alang1033 \ltrch\fcs0 \f31506\fs24\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 {\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid1988182\charrsid1988182 
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0\pararsid1988182 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182\charrsid1988182 
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \b\insrsid9137215\charrsid9137215 References}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \b\insrsid1070915 
\par }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \b\insrsid16392562\charrsid16392562 
\par }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562\charrsid15095093 Anwa}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 r H, Hong S, De Schutter E (}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid16392562\charrsid15095093 ) Controlling Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid16392562\charrsid15095093 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562\charrsid15095093 -activated K}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\super\insrsid16392562\charrsid15095093 +}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562\charrsid15095093  channels with models of Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid16392562\charrsid15095093 2+}{\rtlch\fcs1 \af31507 
\ltrch\fcs0 \f0\insrsid16392562\charrsid15095093  buffering in Purkinje cell.}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562  }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid16392562\charrsid7606648 Cerebellum}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid16392562 , in print.
\par 
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0\pararsid16392562 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562\charrsid16392562 
van Geit W, Achard P, de Schutter E (2007) Neurofitter: a parameter tuning package for a wide range of electrophysiological neuron models. }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid474793\charrsid474793 Front}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\i\f0\insrsid9137215\charrsid9137215  Neuroinform}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562\charrsid16392562  1:1
\par }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 
\par }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562\charrsid16392562 Schmidt H, Stiefel K, Racay P, Schwaller B, Eilers J (2003) Mutational analysis of dendritic Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid9137215\charrsid9137215 2+}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562\charrsid16392562  kinetics in rodent Purkinje cells: role of parvalbumin and calbindin D28k. }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid9137215\charrsid9137215 J Physiol}{\rtlch\fcs1 \af31507 
\ltrch\fcs0 \f0\insrsid16392562\charrsid16392562  551:13-32
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