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A project for image processing and analysis pipelines for plankton sampling

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Plankton ML

This repository contains code and configuration for processing and analysing images of plankton samples. It's experimental, serving as much as a proposed template for new projects than as a project in itself.

It's a companion project to an R-shiny based image annotation app that is not yet released, written by researchers and data scientists at the UK Centre for Ecology and Hydrology in the early stages of a collaboration that was placed on hold.

Installation

Environment and package installation

Using pip

Create a fresh virtual environment in the repository root using Python >=3.12 and (e.g.) venv:

python -m venv venv

Next, install the package using pip:

python -m pip install .

Most likely you are interested in developing and/or experimenting, so you will probably want to install the package in 'editable' mode (-e), along with dev tools and jupyter notebook functionality

python -m pip install -e .[all]

Using conda

Use anaconda or miniconda to create a python environment using the included environment.yml

conda env create -f environment.yml
conda activate cyto_ml

Next install this package without dependencies:

python -m pip install --no-deps -e .

exiftool

We use exiftool to write basic metadata (latitude/longitude of observation, plus timestamp) into individual plankton images extracted from the larger "collage" format that the FlowCam microscope exports them in.

Guidance for installing exiftool

Ubuntu: sudo apt install libimage-exiftool-perl Centos: sudo yum install libimage-exiftool-perl Or in an environment without root access:

git clone https://github.com/exiftool/exiftool.git
export PATH=$PATH:exiftool

Object store

Connection details

.env contains environment variable names for S3 connection details for the JASMIN object store. Fill these in with your own credentials. If you're not sure what the AWS_URL_ENDPOINT should be, please reach out to one of the project contributors listed below.

Object store API

The object_store_api project provides a web-based API to help manage your image data, for use with JASMIN's s3 store.

Please see its documentation for different modes of running the API. The simplest, for single user / testing purposes is:

python src/os_api/api.py

Pipelines

DVC

Please see [DVC.yaml] for notes and walkthroughs on different ways of using Data Version Control both to manage data within a git repository, and to manage sets of scripts as a reproducble pipeline with minimal intervention.

This very basic setup has several stages - build an index of images in an object store (s3 bucket), extract and store their embeddings using a pre-trained neural network, and train and save a classifier based on the embeddings.

cd scripts dvc repro

Luigi

Please see PIPELINES.md for detailed documentation about a pipeline that slices up images exported from a FlowCam instrument, adds spatial and temporal metadata into their EXIF headers based on a directory naming convention agreed with researchers, and uploads them to object storage.

Running tests

pytest or py.test

Contents

Feature extraction

Experiment testing workflows by using this plankton model from SciVision to extract features from images for use in similarity search, clustering, etc.

Running Jupyter notebooks

The notebooks/ directory contains Markdown (.md) representations of the notebooks. To create Jupyter notebooks (.ipynb), run the following command with the conda environment activated:

jupytext --sync notebooks/*

If you modify the contents of a notebook, run the command after closing the notebook to re-sync the .ipynb and .md representations before committing.

For more information see the Jupytext docs.

Visualisation

Streamlit app based off the text embeddings for EIDC catalogue metadata one

streamlit run src/cyto_ml/visualisation/app.py

The demo should automatically open in your browser when you run streamlit. If it does not, connect using: http://localhost:8501.

DAG / pipeline elements

Contributors

Jo Walsh Alba Gomez Segura Ezra Kitson

Contributing

Please see CONTRIBUTING.md

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A project for image processing and analysis pipelines for plankton sampling

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