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RNaseViz is an R Shiny app visualizing RNase sequences, conservation, and mutations linked to Mendelian diseases. It supports interactive analysis and highlights critical mutation impacts across species.

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Norreanea/RNaseViz-RNase-Sequence-Visualization-Tool

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RNaseViz-RNase-Sequence-Visualization-Tool

RNaseViz is a web-based application built with R Shiny, providing an interactive platform for visualizing RNase sequences, their conservation across different species, and the mutations associated with Mendelian diseases. https://norreanea.shinyapps.io/RNaseViz/

Features

  • Selection of RNase enzymes from a predefined list.
  • Visualization of sequence conservation using multiple sequence alignments.
  • Interactive phylogenetic trees illustrating evolutionary relationships.
  • Highlighting specific mutations and their positions within the sequences.
  • Support for various RNase enzymes with associated Mendelian disease mutations.

Usage

To use the RNaseViz application:

  1. Visualize: From the dropdown menu labeled "Choose RNase:", select the RNase enzyme you wish to analyze and click the 'Visualize' button.
  2. Highlight Mutation: Use the "Select organism:" dropdown to choose the organism of interest, enter a position number in the reference sequence, and click 'Highlight position' to view specific mutations.
  3. RNase info: Provides in-depth information about the selected RNase enzyme, including the potential molecular consequences of its mutations.
  4. Residue Highlighting: Highlights the specified residue within the sequence alignment, along with the two adjacent residues on either side, to emphasize key positions.
  5. Explore Tree: The displayed tree structure allows you to explore how different RNase sequences relate to different species.
  6. Inspect Residues: Select and highlight any residue within the sequence alignment for detailed examination and analysis.
  7. Download Global Alignment: Download sequence alignments in FASTA or PDF formats for offline analysis. Figure2

Contact

For questions or support, please open an issue on this GitHub repository.

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RNaseViz is an R Shiny app visualizing RNase sequences, conservation, and mutations linked to Mendelian diseases. It supports interactive analysis and highlights critical mutation impacts across species.

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