From 7891417655eb201b656f1602edb36bba1a9da925 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?M=C3=BCck?= Date: Tue, 7 May 2024 12:52:07 +0200 Subject: [PATCH] added robustness for mixed entry types in ID column of input vcf, created test case --- deeprvat/annotations/annotations.py | 4 +- tests/annotations/test_annotations.py | 81 ++++++++++++ .../vep_deepripe_deepsea_absplice.parquet | Bin 0 -> 27923 bytes .../input/abSplice_score_file.parquet | Bin 0 -> 7351 bytes .../input/vep_deepripe_deepsea.parquet | Bin 0 -> 27164 bytes .../merged_annotations_expected.parquet | Bin 0 -> 286118 bytes .../merge_annotations_mixedIDs/input/test.vcf | 60 +++++++++ .../input/test_hg2_deepripe.csv.gz | Bin 0 -> 23756 bytes .../input/test_k5_deepripe.csv.gz | Bin 0 -> 24329 bytes .../input/test_parclip.csv.gz | Bin 0 -> 19741 bytes .../input/test_vep.tsv | 122 ++++++++++++++++++ .../input/variants.parquet | Bin 0 -> 8910 bytes 12 files changed, 265 insertions(+), 2 deletions(-) create mode 100644 tests/annotations/test_data/merge_absplice_scores/merge_absplice_scores_small/expected/vep_deepripe_deepsea_absplice.parquet create mode 100644 tests/annotations/test_data/merge_absplice_scores/merge_absplice_scores_small/input/abSplice_score_file.parquet create mode 100644 tests/annotations/test_data/merge_absplice_scores/merge_absplice_scores_small/input/vep_deepripe_deepsea.parquet create mode 100644 tests/annotations/test_data/merge_annotations/merge_annotations_mixedIDs/expected/merged_annotations_expected.parquet create mode 100644 tests/annotations/test_data/merge_annotations/merge_annotations_mixedIDs/input/test.vcf create mode 100644 tests/annotations/test_data/merge_annotations/merge_annotations_mixedIDs/input/test_hg2_deepripe.csv.gz create mode 100644 tests/annotations/test_data/merge_annotations/merge_annotations_mixedIDs/input/test_k5_deepripe.csv.gz create mode 100644 tests/annotations/test_data/merge_annotations/merge_annotations_mixedIDs/input/test_parclip.csv.gz create mode 100644 tests/annotations/test_data/merge_annotations/merge_annotations_mixedIDs/input/test_vep.tsv create mode 100644 tests/annotations/test_data/merge_annotations/merge_annotations_mixedIDs/input/variants.parquet diff --git a/deeprvat/annotations/annotations.py b/deeprvat/annotations/annotations.py index b40a7369..72eba673 100644 --- a/deeprvat/annotations/annotations.py +++ b/deeprvat/annotations/annotations.py @@ -1737,8 +1737,8 @@ def process_vep( vcf_file, names=["chrom", "pos", "#Uploaded_variation", "ref", "alt"] ) if "#Uploaded_variation" in vep_file.columns: - vep_file = vep_file.merge(vcf_df, on="#Uploaded_variation") - + vep_file = vep_file.merge(vcf_df, on="#Uploaded_variation", how = 'left') + vep_file.loc[vep_file.chrom.isna(),['chrom','pos','ref','alt']]=vep_file[vep_file['chrom'].isna()]['#Uploaded_variation'].str.replace("_", ":").str.replace("/", ":").str.split(':', expand=True).values if "pos" in vep_file.columns: vep_file["pos"] = vep_file["pos"].astype(int) diff --git a/tests/annotations/test_annotations.py b/tests/annotations/test_annotations.py index 2865978b..83ae128e 100644 --- a/tests/annotations/test_annotations.py +++ b/tests/annotations/test_annotations.py @@ -188,6 +188,18 @@ def test_deepsea_pca( "49", ), + ( + "merge_annotations_mixedIDs", + "merged_annotations_expected.parquet", + "test_hg2_deepripe.csv.gz", + "test_k5_deepripe.csv.gz", + "test_parclip.csv.gz", + "variants.parquet", + "test.vcf", + "test_vep.tsv", + "49", + + ), ] ) @@ -325,3 +337,72 @@ def test_aggregate_abscores( expected_results = pd.read_parquet(expected_path) assert written_results.shape == expected_results.shape assert_frame_equal(written_results, expected_results[written_results.columns], check_exact = False) + + + +@pytest.mark.parametrize( + "test_name_dir, absplice_scores, annotations, expected", + [ + ( "merge_absplice_scores_small", + "abSplice_score_file.parquet", + "vep_deepripe_deepsea.parquet", + "vep_deepripe_deepsea_absplice.parquet", + ), + ] +) +def test_merge_absplice_scores( + test_name_dir, absplice_scores, annotations, expected, tmp_path +): + current_test_data_dir = tests_data_dir / 'merge_absplice_scores' / test_name_dir + absplice_score_path = current_test_data_dir / 'input' / absplice_scores + annotation_path = current_test_data_dir / 'input' / annotations + expected_path = current_test_data_dir / 'expected' / expected + output_path = tmp_path / 'out.parquet' + cli_runner = CliRunner() + cli_parameters = [ + 'merge-abscores', + annotation_path.as_posix(), + absplice_score_path.as_posix(), + output_path.as_posix(), + ] + result = cli_runner.invoke(annotations_cli, cli_parameters, catch_exceptions=False) + assert result.exit_code == 0 + written_results = pd.read_parquet(output_path) + expected_results = pd.read_parquet(expected_path) + assert written_results.shape == expected_results.shape + assert_frame_equal(written_results, expected_results, check_exact = False) + + +# @pytest.mark.parametrize( +# "test_name_dir, input_file_1, input_file_2, parameter1, expected", +# [ +# ( "test_name_dir", +# "input_file1.parquet", +# "input_file2.parquet", +# "8", +# "expected.parquet", +# ), +# ] +# ) +# def template( +# test_data_name_dir, input_file_1, input_file_2, parameter1, expected, tmp_path +# ): +# current_test_data_dir = tests_data_dir / 'test_name' / test_data_name_dir +# input_path1 = current_test_data_dir / 'input' / input_file_1 +# input_path2 = current_test_data_dir / 'input' /input_file_2 +# expected_path = current_test_data_dir / 'expected' / expected +# output_path = tmp_path / 'out.parquet' +# cli_runner = CliRunner() +# cli_parameters = [ +# 'function-name', +# input_path1.as_posix(), +# input_path2.as_posix(), +# output_path.as_posix(), +# parameter1, +# ] +# result = cli_runner.invoke(annotations_cli, cli_parameters, catch_exceptions=False) +# assert result.exit_code == 0 +# written_results = pd.read_parquet(output_path) +# expected_results = pd.read_parquet(expected_path) +# assert written_results.shape == expected_results.shape +# assert_frame_equal(written_results, expected_results[written_results.columns], check_exact = False) diff --git a/tests/annotations/test_data/merge_absplice_scores/merge_absplice_scores_small/expected/vep_deepripe_deepsea_absplice.parquet b/tests/annotations/test_data/merge_absplice_scores/merge_absplice_scores_small/expected/vep_deepripe_deepsea_absplice.parquet new file mode 100644 index 0000000000000000000000000000000000000000..e3fa0da4a68cc62aa627af61c8e9c368020b5139 GIT binary patch literal 27923 zcmd5l3wTrI*(ar?0SZXL_kQpDr8E>m3s#0wFd}(2e+1M&6C|g)|NK|%2 zgxBHX&jNY=av&Fz6ku>Eg%1pa`z<2Lc2T2bF2z8@dDH#dEE_h2RwT}SZ~r|XDnp6$ zdry34bTpdy`4@Ub`)X@6D^498e%t3uh)wqyN6-5c5B#9yhU9`diF02v-_zAvlh9lj zo0W(JkpwCvi`f!jdZc0oW*N3r%x?v{N>?H37K#dsutiE;Xz*)d{A5u^4zp})%xyis z-T)ctp~P{tpzL@7ez*|->NF4tA7lgw% zm17vA0*s|{b}K-bQIS&lLJU7$n&G5EK;gmeSa)xhz7L4Xk|O-MtMJ`drS;85kkL20 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C T +chr3 6134790 rs881 A G +chr3 6492413 . A G +chr3 7479092 . T A +chr3 10151779 . G T +chr3 10963200 . 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