Identify Differential Expressed Genes in DgcA null mutant and Knock-out samples in Dictyostelium Discoideum. The same workflow has been used for the differential expression analysis of Polysphondylium pallidum.
https://github.com/bartongroup/rs-Dictostelium_Differential_Expression_Experiment
R CMD BATCH QualityControl.R &
./try.sh
./try1.sh
./try2.sh
R CMD BATCH InputIGV-HtseqCount.R
R CMD BATCH htseq-count.R
R CMD BATCH DiffExpTwoGroup.R
R CMD BATCH GSEA.R
Chen ZH, Singh R, Cole C, Lawal HM, Schilde C, Febrer M, Barton GJ, Schaap P. Adenylate cyclase A acting on PKA mediates induction of stalk formation by cyclic diguanylate at the Dictyostelium organizer. Proceedings of the National Academy of Sciences. 2017 Jan 17;114(3):516-21.