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Update README.md
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RobertMolenaar-UT authored Nov 28, 2024
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Expand Up @@ -3,7 +3,7 @@ Create fastFLIM images from PicoQuant PTU files.

![A2_Shep2_20__FLIM_Ch1→2_cr](https://github.com/user-attachments/assets/5f02dfff-c8ec-4779-b356-24f447a1fb1a)

*Figure 1, Example of a 1 channel fastFLIM converted image, of cells labelled with FluoVolt dye, that shows change in lifetime, Shep2 cell made by E. de Jong phD..*
*Figure 1, Example of a 1 channel fastFLIM converted image, of cells labelled with FluoVolt dye, that shows change in lifetime, Shep2 cell made by E. de Jong PhD..*

## Discription
The main purpose of the fastFLIM script is one can convert by batch multiple PTU files, or a folder with PTU files, and get a series Fluorescent fastFLIM images with minimal user input. This is usefull for screening results during imaging and to be used in presentations. FastFLIM calculates the average arrival time, which is a fast method to get a fluorescent lifetime information for image with low photon counts per pixel, which is typical for TCSPC methods.
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