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outcomes-en.html
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<!DOCTYPE html>
<html lang="en">
<head>
<meta charset="utf-8">
<meta name="viewport" content="width=device-width, initial-scale=1, shrink-to-fit=no">
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<meta name="application-name" content="SJTU-BioX-Shanghai">
<meta name="description" content="Outcomes of SJTU-BioX-Shanghai, the iGEM team of Shanghai Jiao Tong University">
<meta name="keywords" content="SJTU,Bio-X,synthetic biology,biology,iGEM,iGEM competition,Shanghai Jiao Tong University">
<meta name="Author" Content="Wazon,Wazons@163.com">
<title>Outcomes - SJTU-BioX-Shanghai</title>
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<h2 class="h2-responsive wow fadeIn">Patents</h2>
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<div class="col-sm-4 col-lg-3 order-2 order-sm-1 align-self-top">
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<img src="./img/jpg/patent2.jpg" class="img-fluid" alt="专利证书照片1">
<a target="_blank" href="http://cpquery.sipo.gov.cn/">
<div class="mask waves-effect waves-light"></div>
</a>
</div>
<div class="card-body text-center">
<p class="card-text">A method for optimizing the pathway of fatty acid synthesis in E. coli</p>
</div>
</div>
<p> </p>
</div>
<div class="col-sm-4 col-lg-3 order-3 order-sm-3 align-self-top">
<div class="card wow fadeIn" data-wow-delay="0s">
<div class="view overlay hm-white-my-light">
<img src="./img/jpg/patent1.jpg" class="img-fluid" alt="专利证书照片2">
<a target="_blank" href="http://cpquery.sipo.gov.cn/">
<div class="mask waves-effect waves-light"></div>
</a>
</div>
<div class="card-body text-center">
<p class="card-text">A system for efficient production of fatty acids by E. coli and a method for constructing it</p>
</div>
</div>
<p> </p>
</div>
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<h2 class="h2-responsive wow fadeIn">Papers</h2>
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<div class="col-12 col-md-10">
<h4 class="h4-responsive">An Engineered Rare Codon Device for Optimization of Metabolic Pathways</h4>
<p>Rare codons generally arrest translation due to rarity of their cognate tRNAs. This property of rare codons can be utilized to regulate protein expression. In this study, a linear relationship was found between expression levels of genes
and copy numbers of rare codons inserted within them. Based on this discovery, we constructed a molecular device in Escherichia coli using the rare codon AGG, its cognate tRNA (tRNA<sup>Arg</sup> (CCU)), modified tRNA<sup>Asp</sup> (GUC → CCU), and truncated aspartyl-tRNA synthetase (TDRS) to switch the expression of reporter genes on or off as well as to precisely regulate their expression to various intermediate levels. To underscore the applicability of our
work, we used the rare codon device to alter the expression levels of four genes of the fatty acid synthesis II (FASII) pathway (i.e. fabZ, fabG, fabI, and tesA’) in E. coli to optimize steady-state kinetics, which produced nearly
two-fold increase in fatty acid yield. Thus, the proposed method has potential applications in regulating target protein expression at desired levels and optimizing metabolic pathways by precisely tuning in vivo molar ratio of relevant
enzymes.
</p>
<p>
<a target="_blank" href="https://www.nature.com/articles/srep20608"> <i class="fa fa-external-link" aria-hidden="true"></i> Details（nature.com） </a>
</p>
<h4 class="h4-responsive" style="margin-top:3rem;">Quantitatively Relating Gene Expression to Light Intensity via the Serial Connection of Blue Light Sensor and CRISPRi</h4>
<p>The ability to regulate endogenous gene expression is critical in biological research. Existing technologies, such as RNA interference, zinc-finger regulators, transcription-activator-like effectors, and CRISPR-mediated regulation, though
proved to be competent in significantly altering expression levels, do not provide a quantitative adjustment of regulation effect. As a solution to this problem, we place CRISPR-mediated interference under the control of blue light:
while dCas9 protein is constitutively expressed, guide RNA transcription is regulated by YF1-FixJ-PFixK2, a blue light responding system. With a computer-controlled luminous device, the quantitative relationship between target gene
expression and light intensity has been determined. As the light intensifies, the expression level of target gene gradually ascends. This remarkable property enables sensor-CRISPRi to accurately interrogate cellular activities.</p>
<p>
<a target="_blank" href="http://pubs.acs.org/doi/abs/10.1021/sb500059x"> <i class="fa fa-external-link" aria-hidden="true"></i> Details（acs.org） </a>
</p>
</div>
</div>
<div class="divider-new">
<h2 class="h2-responsive wow fadeIn">Team composition and whereabouts</h2>
</div>
<div class="row">
<div class="col-12 d-flex flex-column justify-content-center ">
<table class="table table-res table-res1 table-hover" align="center">
<thead class="blue darken-3">
<tr class="text-white">
<th>Year</th>
<th>Participants</th>
<th>Official members</th>
<th>Further studies</th>
<th>Study abroad</th>
</tr>
</thead>
<tbody>
<tr>
<th scope="row">2009</th>
<td>47</td>
<td>18</td>
<td>14</td>
<td>10</td>
</tr>
<tr>
<th scope="row">2010</th>
<td>65</td>
<td>15</td>
<td>12</td>
<td>8</td>
</tr>
<tr>
<th scope="row">2011</th>
<td>60</td>
<td>9</td>
<td>7</td>
<td>6</td>
</tr>
<tr>
<th scope="row">2012</th>
<td>50</td>
<td>11</td>
<td>9</td>
<td>8</td>
</tr>
<tr>
<th scope="row">2013</th>
<td>80</td>
<td>11</td>
<td>8</td>
<td>6</td>
</tr>
<tr>
<th scope="row">2014</th>
<td>50</td>
<td>11</td>
<td>7</td>
<td>5</td>
</tr>
<tr>
<th scope="row">2015</th>
<td>50</td>
<td>10</td>
<td>5</td>
<td>4</td>
</tr>
</tbody>
</table>
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