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outcomes.html
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<!DOCTYPE html>
<html lang="zh-CN">
<head>
<meta charset="utf-8">
<meta name="viewport" content="width=device-width, initial-scale=1, shrink-to-fit=no">
<meta http-equiv="x-ua-compatible" content="ie=edge">
<meta name="application-name" content="SJTU-BioX-Shanghai">
<meta name="description" content="SJTU-BioX-Shanghai在比赛比赛之外获得的成果">
<meta name="keywords" content="SJTU,Bio-X,合成生物学,生物学,iGEM,国际遗传工程机器大赛,上海交通大学">
<meta name="Author" Content="Wazon,Wazons@163.com">
<title>衍生成果 - SJTU-BioX-Shanghai</title>
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<style>
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line-height: 1.25em;
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<body class="grey lighten-5" data-spy="scroll" data-target="#navbar-right" data-offset="100">
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<div class="container">
<a class="navbar-brand" href="#"><img src="./img/png/logo.png" height="55" /></a>
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<div class="collapse navbar-collapse" id="navbarNav1">
<ul class="navbar-nav mr-auto">
<li class="nav-item"><a href="./index.html" class="nav-link">首页</a></li>
<li class="nav-item"><a href="./intro.html" class="nav-link">团队介绍</a></li>
<li class="nav-item"><a href="./history.html" class="nav-link">参赛历程</a></li>
<li class="nav-item"><a href="./practices.html" class="nav-link">社会活动</a></li>
<li class="nav-item active"><a href="./outcomes.html" class="nav-link">衍生成果<span class="sr-only">(current)</span></a></li>
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<ul class="navbar-nav ml-auto">
<li class="nav-item dropdown"><a href="./outcomes-en.html" class="nav-link">ENGLISH</a></li>
</ul>
</div>
</div>
</nav>
<div class="container" style="margin-top:56px;">
<div class="divider-new">
<h2 class="h2-responsive wow fadeIn">国内荣誉</h2>
</div>
<div class="row small-row justify-content-center">
<div class="col-sm-4 col-lg-3 order-2 order-sm-1 align-self-center">
<div class="card wow fadeIn" data-wow-delay="0s">
<div class="view overlay hm-white-my-light">
<img src="./img/png/qianxuesen.png" class="img-fluid" alt="钱学森杯">
</div>
<div class="card-body text-center">
<h4 class="card-title">“钱学森杯” 特等奖</h4>
<p class="card-text"><span>第二届“钱学森杯”</span><span>课外学术</span><span>科技作品竞赛</span></p>
</div>
</div>
<p> </p>
</div>
<div class="col-sm-4 col-lg-3 order-1 order-sm-2 align-self-center">
<div class="card wow fadeIn" data-wow-delay="0s">
<div class="view overlay hm-white-my-light">
<img src="./img/jpg/tiaozhanbei.jpg" class="img-fluid" alt="“挑战杯”获奖照片">
<a target="_blank" href="http://news.sjtu.edu.cn/info/1009/153362.htm">
<div class="mask waves-effect waves-light"></div>
</a>
</div>
<div class="card-body text-center">
<h4 class="card-title">“挑战杯” 特等奖</h4>
<p class="card-text"><span>2013 年</span><span>全国总决赛</span></p>
<p class="card-text"><span>生命科学组</span><span>特等奖</span></p>
</div>
</div>
<p> </p>
</div>
<div class="col-sm-4 col-lg-3 order-3 order-sm-3 align-self-center">
<div class="card wow fadeIn" data-wow-delay="0s">
<div class="view overlay hm-white-my-light">
<img src="./img/jpg/letter.jpg" class="img-fluid" alt="钱学森杯">
</div>
<div class="card-body text-center">
<h4 class="card-title">校长贺信</h4>
<p class="card-text"><span>称赞团队为</span><span>交通大学</span><span>乃至亚洲高校</span><span>赢得了荣誉</span></p>
</div>
</div>
<p> </p>
</div>
</div>
<div class="divider-new">
<h2 class="h2-responsive wow fadeIn">发明专利</h2>
</div>
<div class="row small-row justify-content-center">
<div class="col-sm-4 col-lg-3 order-2 order-sm-1 align-self-top">
<div class="card wow fadeIn" data-wow-delay="0s">
<div class="view overlay hm-white-my-light">
<img src="./img/jpg/patent2.jpg" class="img-fluid" alt="专利证书照片1">
<a target="_blank" href="http://cpquery.sipo.gov.cn/">
<div class="mask waves-effect waves-light"></div>
</a>
</div>
<div class="card-body text-center">
<p class="card-text"><span>一种优化</span><span>大肠杆菌脂肪酸</span><span>合成途径的方法</span></p>
</div>
</div>
<p> </p>
</div>
<div class="col-sm-4 col-lg-3 order-3 order-sm-3 align-self-top">
<div class="card wow fadeIn" data-wow-delay="0s">
<div class="view overlay hm-white-my-light">
<img src="./img/jpg/patent1.jpg" class="img-fluid" alt="专利证书照片2">
<a target="_blank" href="http://cpquery.sipo.gov.cn/">
<div class="mask waves-effect waves-light"></div>
</a>
</div>
<div class="card-body text-center">
<p class="card-text"><span>一种大肠杆菌</span><span>高效生产脂肪酸的</span><span>系统及其构建方法</span></p>
</div>
</div>
<p> </p>
</div>
</div>
<div class="divider-new">
<h2 class="h2-responsive wow fadeIn">团队论文</h2>
</div>
<div class="row justify-content-center">
<div class="col-12 col-md-10">
<h4 class="h4-responsive">An Engineered Rare Codon Device for Optimization of Metabolic Pathways</h4>
<p>Rare codons generally arrest translation due to rarity of their cognate tRNAs. This property of rare codons can be utilized to regulate protein expression. In this study, a linear relationship was found between expression levels of genes
and copy numbers of rare codons inserted within them. Based on this discovery, we constructed a molecular device in Escherichia coli using the rare codon AGG, its cognate tRNA (tRNA<sup>Arg</sup> (CCU)), modified tRNA<sup>Asp</sup> (GUC → CCU), and truncated aspartyl-tRNA synthetase (TDRS) to switch the expression of reporter genes on or off as well as to precisely regulate their expression to various intermediate levels. To underscore the applicability of our
work, we used the rare codon device to alter the expression levels of four genes of the fatty acid synthesis II (FASII) pathway (i.e. fabZ, fabG, fabI, and tesA’) in E. coli to optimize steady-state kinetics, which produced nearly
two-fold increase in fatty acid yield. Thus, the proposed method has potential applications in regulating target protein expression at desired levels and optimizing metabolic pathways by precisely tuning in vivo molar ratio of relevant
enzymes.
</p>
<p>
<a target="_blank" href="https://www.nature.com/articles/srep20608"> <i class="fa fa-external-link" aria-hidden="true"></i> 论文详情（nature.com） </a>
</p>
<h4 class="h4-responsive" style="margin-top:3rem;">Quantitatively Relating Gene Expression to Light Intensity via the Serial Connection of Blue Light Sensor and CRISPRi</h4>
<p>The ability to regulate endogenous gene expression is critical in biological research. Existing technologies, such as RNA interference, zinc-finger regulators, transcription-activator-like effectors, and CRISPR-mediated regulation, though
proved to be competent in significantly altering expression levels, do not provide a quantitative adjustment of regulation effect. As a solution to this problem, we place CRISPR-mediated interference under the control of blue light:
while dCas9 protein is constitutively expressed, guide RNA transcription is regulated by YF1-FixJ-PFixK2, a blue light responding system. With a computer-controlled luminous device, the quantitative relationship between target gene
expression and light intensity has been determined. As the light intensifies, the expression level of target gene gradually ascends. This remarkable property enables sensor-CRISPRi to accurately interrogate cellular activities.</p>
<p>
<a target="_blank" href="http://pubs.acs.org/doi/abs/10.1021/sb500059x"> <i class="fa fa-external-link" aria-hidden="true"></i> 论文详情（acs.org） </a>
</p>
</div>
</div>
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