Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Bin reads by mapping to transcriptome #16

Open
kbseah opened this issue Oct 22, 2020 · 0 comments
Open

Bin reads by mapping to transcriptome #16

kbseah opened this issue Oct 22, 2020 · 0 comments
Labels
enhancement New feature or request

Comments

@kbseah
Copy link
Member

kbseah commented Oct 22, 2020

Like MILCOR but with transcriptome as reference instead, e.g. for use case where no reference genome is available.

Distinguish introns from IESs by GT/AG boundaries, and 100% "retention" of introns (vs. IESs, if the library is only an enrichment).

However splicing may result in erroneous indel calls; for minimap2 mapper, "splice" mode is not suitable for our needs because it assumes RNAseq reads are being mapped onto genomic reference, not the other way around. What we could do is to compare predicted intron positions on different isoforms, if available.

@kbseah kbseah added the enhancement New feature or request label Oct 22, 2020
Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
enhancement New feature or request
Projects
None yet
Development

No branches or pull requests

1 participant