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Update README.md
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TLenfers authored Mar 30, 2022
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Expand Up @@ -65,7 +65,7 @@ snakemake -j n all --use-conda --use-singularity
# To run human variant analysis with mutserve
snakemake -j n all_human --use-conda --use-singularity
# where n is the numer of cores to use
```
```m
## Output
Expand All @@ -81,8 +81,8 @@ The results are in a sub-folder corresponding to the name of the reference file
- `/results/calls_mutserve` contains all called variants using mutserve.
- `/results/mapped` contains all aligned reads as `sample_name.bam` ad their index file `sample_name.bam.bai`.
- `/results/plots` contains the created heatmap plots for the bctools caller. Example plots:
- [ref_heatmap.pdf](https://github.com/TLenfers/multispecies_mitochondrial_variant_analysis/files/8379593/ref_heatmap.pdf)
- [ref_heatmap_clusterrow.pdf](https://github.com/TLenfers/multispecies_mitochondrial_variant_analysis/files/8379594/ref_heatmap_clusterrow.pdf)
- [ref_heatmap.pdf](https://github.com/TLenfers/multispecies_mitochondrial_variant_analysis/files/8382839/ref_heatmap.pdf)
- [ref_heatmap_clusterrow.pdf](https://github.com/TLenfers/multispecies_mitochondrial_variant_analysis/files/8382840/ref_heatmap_clusterrow.pdf)
- The name of the samples is on the X-axis, the variants on the Y-axis
- The values of the heatmap refer to the Phred-scaled likelihood for homomorphic reference allele (scale 0-255; 255: reference is very unlikely -> alternative more likely).
- The plots of `alt_heatmap` are containing the Phred-scaled likelihood for homomorphic alternative allele, i.e. that the variant is present at this position (scale 0-255; 0: variant is present).
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