V1.0.1 (#5)

* Downgrading requirement to matplotlib 3.8.3 due to long loading time with matplotlib 3.9

* Updating notebooks and adding MEA notebook

* Adding images

* Update README.md

README.md

@@ -1,21 +1,27 @@
 <div align="center">
     <picture>
-        <img src="./src/notebooks/images/logo_qr_combined.png" alt="The Kinase Library" width="50%">
+        <img src="https://github.com/TheKinaseLibrary/kinase-library/raw/v1.0.1/src/notebooks/images/logo_qr_combined.png" alt="The Kinase Library" width="50%">
     </picture>
 
 <hr/>
 
+# [Click here for The Kinase Library Web Tool](https://kinase-library.phosphosite.org)
+
 [![Twitter Follow](https://img.shields.io/twitter/follow/KinaseLibrary?style=social)](https://twitter.com/KinaseLibrary) &ensp;
 [![License: CC BY-NC-SA 3.0](https://img.shields.io/badge/License-CC%20BY--NC--SA%203.0-lightgrey.svg)](https://creativecommons.org/licenses/by-nc-sa/3.0/) &ensp;
 [![PyPI Latest Release](https://img.shields.io/pypi/v/kinase-library.svg)](https://pypi.org/project/kinase-library/)
 
 </div>
 
-The Kinase Library Python package provides tools for analyzing kinase phosphorylation preferences and predicting substrate specificity. This package is based on recent research that leverages bioinformatics and synthetic peptide libraries to identify potential phosphorylation sites for both Serine/Threonine and Tyrosine kinases. It includes useful functions and analyses, such as:
+**The Kinase Library** is a comprehensive Python package for analyzing phosphoproteomics data, focusing on kinase-substrate relationships. It provides tools for kinase prediction, enrichment analysis, and visualization, enabling researchers to gain insights into kinase activities and signaling pathways from phosphoproteomics datasets.
+
+## Features
 
-- **Score Site and Multiple Sites**: Calculate scores for phosphorylation at specific sites or across multiple potential sites.
-- **Binary Enrichment Analysis**: Kinase enrichment analysis based on Fisher's exact test for foreground and background substrate lists
-- **Differential Phosphorylation Analysis**: Kinase enrichment analysis based on Fisher's exact test for differentially phosphorylated phosphosites
+* **Kinase Prediction**: Predict potential kinases responsible for phosphorylation sites using a built-in kinase-substrate prediction algorithm.
+* **Enrichment Analysis**: Perform kinase enrichment analysis using binary enrichment or differential phosphorylation analysis.
+* **Motif Enrichment Analysis (MEA)**: Identify kinases potentially regulated in your dataset using MEA with the GSEA algorithm.
+* **Visualization**: Generate volcano plots, bubble maps, and other visualizations to interpret enrichment results.
+* **Downstream Substrate Identification**: Explore putative downstream substrates of enriched kinases.
 
 ## Installation
 
@@ -27,7 +33,7 @@ pip install kinase-library
 
 ## Getting Started
 
-The Kinase Library package offers several tools for analyzing kinase phosphorylation sites. Below are some examples to help you get started. See [`src/notebooks`](https://github.com/TheKinaseLibrary/kinase-library/tree/master/src/notebooks/) for more comprehensive usage.
+The Kinase Library package offers several tools for analyzing kinase phosphorylation sites. Below are some basic examples to help you get started. Please refer to [`Notebooks`](https://github.com/TheKinaseLibrary/kinase-library/tree/master/src/notebooks/) for more comprehensive usage.
 
 ### Example: Identify kinases capable of phosphorylating a site using `Substrate`
 
@@ -63,7 +69,7 @@ Here’s an example of the output you can expect from using the Substrate.predic
 
 ### Example: Identify kinases capable of phosphorylating a site for multiple sites using `PhosphoProteomics`
 
-Let's say you have a CSV file called "pps_data.csv" containing the following list of phosphosites:
+Assuming you have a CSV file called "pps_data.csv" containing the following list of phosphosites:
 
 ```
 uniprot,protein,gene,description,position,residue,best_localization_prob,sequence window
@@ -78,12 +84,12 @@ Q02790,FKBP4,FKBP4,Peptidyl-prolyl cis-trans isomerase FKBP4,336,S,0.999938,PDRR
 import kinase_library as kl
 import pandas as pd
 
-phosphosites_data = pd.read_csv("pps_data.csv")
+phosphosites_data = pd.read_csv('pps_data.csv')
 pps = kl.PhosphoProteomics(phosphosites_data, seq_col='sequence window')
 pps.predict(kin_type='ser_thr')
 ```
 
-Here’s an example of the output you can expect from using the PhosphoProteomics.predict() function.
+This is the expected output from using the PhosphoProteomics.predict() function.
 
 <div style="overflow-x:auto;">
 
@@ -101,18 +107,12 @@ Here’s an example of the output you can expect from using the PhosphoProteomic
 
 Please cite the following papers when using this package:
 
-- "An atlas of substrate specificities for the human serine/threonine kinome"
-  Johnson, J.L., et al., Nature, 2023 Jan 11; 613, 759–766; DOI: [10.1038/s41586-022-05575-3](https://doi.org/10.1038/s41586-022-05575-3)
+**For the serine/threonine kinome:**
+> Johnson, J. L., Yaron, T. M., Huntsman, E. M., Kerelsky, A., Song, J., Regev, A., ... & Cantley, L. C. (2023). **An atlas of substrate specificities for the human serine/threonine kinome**. _Nature_, 613(7945), 759-766. [http://doi.org/10.1074/mcp.TIR118.000943](https://doi.org/10.1038/s41586-022-05575-3)
 
-- "The intrinsic substrate specificity of the human tyrosine kinome"
-  Yaron-Barir, T.M., et al., Nature, 2024 May 8; 629, 1174-1181; DOI: [10.1038/s41586-024-07407-y](https://doi.org/10.1038/s41586-024-07407-y)
+**For the tyrosine kinome:**
+> Yaron-Barir, T. M., Joughin, B. A., Huntsman, E. M., Kerelsky, A., Cizin, D. M., Cohen, B. M., ... & Johnson, J. L. (2024). **The intrinsic substrate specificity of the human tyrosine kinome**. _Nature_, 1-8. [https://doi.org/10.1038/s41586-024-07407-y](https://doi.org/10.1038/s41586-024-07407-y)
 
 ## License
 
 This package is distributed under the Creative Commons License. See `LICENSE` for more information.
-
-## Contributors
-
-<a href="https://github.com/TheKinaseLibrary/kinase-library/graphs/contributors" alt="View Contributors">
-  <img src="https://contrib.rocks/image?repo=TheKinaseLibrary/kinase-library&max=1000&columns=10" alt="Contributors" />
-</a>