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AMPtk_code_and_results
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AMPtk_code_and_results
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$ cd ~/BlodgettAmpliconSequencingRawData/
$ conda activate amptk_v1.5.1
$ amptk illumina -i allITS -o allITS_AMPTKpreprocessed -f AACTTTYRRCAAYGGATCWCT -r AGCCTCCGCTTATTGATATGCTTAART --rescue_forward off --primer_mismatch 6
-------------------------------------------------------
[Jan 06 01:37 PM]: OS: MacOSX 10.15.6, 4 cores, ~ 8 GB RAM. Python: 3.7.8
[Jan 06 01:37 PM]: AMPtk v1.5.1, VSEARCH v2.15.0
[Jan 06 01:37 PM]: Gzipped files detected, uncompressing
[Jan 06 01:54 PM]: AACTTTYRRCAAYGGATCWCT fwd primer not found in AMPtk primer db, assuming it is actual primer sequence.
[Jan 06 01:54 PM]: AGCCTCCGCTTATTGATATGCTTAART rev primer not found in AMPtk primer db, assuming it is actual primer sequence.
[Jan 06 01:54 PM]: Demuxing data using 4 cpus
[Jan 06 01:54 PM]: Dropping reads less than 100 bp and setting lossless trimming to 300 bp.
[Jan 06 01:54 PM]: Warning: --require_primer=on, ensure that your reads will contain --fwd_primer
[Jan 06 01:54 PM]: Strip Primers and Merge PE reads. FwdPrimer: AACTTTYRRCAAYGGATCWCT RevPrimer: AGCCTCCGCTTATTGATATGCTTAART
-------------------------------------------------------
[Jan 06 03:37 PM]: Concatenating Demuxed Files
[Jan 06 03:49 PM]: 39,825,189 total reads
[Jan 06 03:49 PM]: 39,545,575 Fwd Primer found, 39,236,738 Rev Primer found
[Jan 06 03:49 PM]: 481,368 discarded Primer incompatibility
[Jan 06 03:49 PM]: 5,131,108 discarded too short (< 100 bp)
[Jan 06 03:49 PM]: 29,899,194 valid output reads
[Jan 06 03:52 PM]: Found 671 barcoded samples
Sample: Count
O321-2-REP2-ITS-O240-P1-REP2-16S: 141438
BS339-400-top-5L-ITS: 127964
O321-2-rep4-ITS: 118785
F240-1-REP1-ITS: 114909
O321-4-REP2-ITS-F240-10-REP6-16S: 113885
D321-20-REP6-ITS: 113051
F240-10-rep5-ITS: 112400
D321-2-REP6-ITS: 109737
F240-2-REP3-ITS: 108929
O321-5-REP4-ITS-D321-4-REP5-16S: 103297
F321-5-REP5-ITS: 103289
F240-2-REP5-ITS: 102152
O321-3-REP3-ITS: 101238
D321-3-REP3-ITS: 99726
F240-20-REP3-ITS: 99654
O321-10-rep2-ITS: 98891
F321-4-REP5-ITS: 98685
F240-5-REP3-ITS: 97688
D321-4-rep6-ITS: 96904
F321-3-REP3-ITS-D321-1-REP2-16S: 96814
F321-2-REP4-ITS: 96615
F240-10-REP4-ITS: 95581
BS310-240-10CM-ITS: 94963
O240-P2-REP2-ITS: 94472
D240-10-REP2-ITS: 93117
F321-4-REP2-ITS: 92608
O321-4-REP4-ITS: 91948
D321-3-REP1-ITS: 90398
D240-1-REP2-ITS: 90147
F240-10-REP1-ITS: 90089
O321-20-REP1-ITS: 90087
F321-20-REP6-ITS-O321-3-REP3-16S: 88858
BS192-321E-top-2-7d-ITS: 87885
O321-4-REP5-ITS: 86803
F240-P2-REP3-ITS: 86376
F321-P1-REP5-ITS: 85914
F240-P2-REP6-ITS: 85252
O321-20-REP5-ITS: 85232
F321-1-REP5-ITS: 85119
D321-4-REP5-ITS: 84968
O321-1-REP4-ITS: 84760
D240-20-REP5-ITS: 83704
O321-10-REP6-ITS: 81853
O240-P1-REP1-ITS: 81573
BS323-321WB-ITS: 81438
BS307-321W-ITS: 80330
F240-20-REP4-ITS: 80324
O240-3-rep4-ITS: 80125
O321-P2-REP1-ITS: 79926
O240-P2-REP6-ITS: 79306
O321-20-REP2-ITS: 78729
F240-10-REP3-ITS: 78099
D321-10-REP4-ITS: 77672
O321-1-REP2-ITS: 77606
O240-2-REP6-ITS: 77064
F240-10-REP6-ITS: 76529
D240-1-rep1-ITS: 75563
D321-20-REP4-ITS: 75531
F321-4-REP1-ITS: 75013
D321-1-REP2-ITS: 74916
BS347-321W5m-top-4u-ITS: 74024
D321-5-REP6-ITS: 73560
O321-p2-rep2-ITS: 72993
B34-ITS: 72655
F240-3-REP3-ITS: 72492
O240-20-REP6-ITS: 72324
BS217-321W-10cm-7-1d-ITS: 71303
F321-3-rep1-ITS: 71266
BS330-321W10MT-ITS: 71082
BS346-321W5MB-ITS: 70806
O321-P2-REP3-ITS: 70788
BS147-240-10cm-1-9d-ITS: 70046
BS303-321EB-ITS: 69960
D240-20-REP3-ITS: 69708
F240-P2-REP5-ITS: 69606
F321-2-REP3-ITS: 69584
O240-3-REP5-ITS: 69493
F240-3-REP5-ITS: 68825
F240-3-REP1-ITS: 68354
F240-P1-REP6-ITS: 68291
BS234-321W-10cm-4-3d-ITS: 68258
F321-5-REP2-ITS: 68177
B32-ITS: 67835
BS146-321W-10cm-3-7u-ITS: 67739
O321-2-rep1-ITS: 67486
D240-1-REP3-ITS: 67477
BS341-400T-ITS: 67337
O240-5-REP4-ITS-F321-1-REP5-16S: 67106
O321-P2-REP5-ITS: 66848
BS30-321E-bottom-1-5u-ITS: 66763
O240-10-rep3-ITS: 66730
O240-10-REP6-ITS: 66635
B35-ITS: 66614
O240-P1-REP4-ITS: 66368
BS165-240-10cm-8-3d-ITS: 65937
F240-P1-REP5-ITS: 65871
F240-5-REP5-ITS: 65848
BS199-321W-10cm-8-6u-ITS: 65242
O321-1-rep5-ITS: 64580
O240-3-REP2-ITS: 64110
D321-10-REP6-ITS: 63975
BS32-321W-10cm-3-4u-ITS: 63685
BS71-321W-10cm-9-1d-ITS: 63604
BS250-321E-top-7-8u-ITS: 63588
O321-P1-REP1-ITS: 63461
O240-2-REP5-ITS-O321-4-REP4-16S: 63317
BS15-380-top-6u-ITS: 63316
BS348-321W5m-bottom-4u-ITS: 63220
BS293-240-10cm-4d-ITS: 62724
O240-P1-REP2-ITS: 62211
D321-1-rep5-ITS: 62201
O240-10-REP5-ITS: 62199
BS157-321E-bottom-2-6d-ITS: 62111
BS266-321E-top-6-6u-ITS: 61934
BS319-321W-bottom-2-8u-ITS: 60919
BS333-321W10MB-ITS: 60679
BS271-321W-10cm-2d-ITS: 60589
D240-20-rep1-ITS: 60577
BS27-321E-top-1u-ITS: 60324
O240-5-rep5-ITS: 60167
BS184-240-10cm-0-9u-ITS: 60154
BS213-321E-bottom-5-9u-ITS: 59821
BS17-380-top-9u-ITS: 59405
BS285-321E-bottom-4-4u-ITS: 59184
BS164-321W-10cm-9-4d-ITS: 58958
D321-4-REP4-ITS: 58619
D321-3-rep4-ITS: 58556
BS204-400-top-7-8R-ITS: 58475
F321-20-REP3-ITS: 57664
O240-2-rep4-ITS: 57580
D321-4-REP2-ITS: 57328
F240-P2-REP4-ITS: 57266
BS345-321W5m-top-2d-ITS: 57168
BS318-321W-top-2-8u-ITS: 56600
O321-p1-rep6-ITS: 56558
D321-1-rep6-ITS: 56527
BS110-400-10cm-rep1-ITS: 56427
F240-3-rep6-ITS: 56354
O321-1-rep6-ITS: 56230
F321-10-rep2-ITS: 56174
F240-5-rep6-ITS: 55863
O240-3-REP6-ITS: 55621
BS138-321E-top-6-3d-ITS: 55524
BS197-321E-bottom-5-3u-ITS: 55368
BS69-321E-bottom-7-3u-ITS: 55011
BS214-321E-top-8-4u-ITS: 54913
D321-10-rep1-ITS: 54511
BS161-321E-bottom-4-9u-ITS: 54414
BS289-321W-10cm-2-2d-ITS: 54385
BS94-321W-10cm-3-4d-ITS: 54121
B33-ITS: 54117
F321-10-rep4-ITS: 53870
BS24-321E-bottom-9u-ITS: 53722
BS329-321E-bottom-1-7u-ITS: 53485
F321-1-rep4-ITS: 53252
BS180-321W-10cm-0-4d-ITS: 53240
F321-p2-rep3-ITS: 53057
BS127-321W-10cm-7-7d-ITS: 52963
BS20-321E-bottom-3u-ITS: 52817
O240-p1-rep5-ITS: 52753
BS19-321E-top-3u-ITS: 52697
O240-P1-REP3-ITS: 52460
BS254-321W-10cm-0-3d-ITS: 52282
BS238-240-10cm-1-8u-ITS: 52168
BS326-321E-top-0-5u-ITS: 52102
BS139-321E-bottom-6-3d-ITS: 51894
BS62-240-10cm-8-1d-ITS: 51780
D321-3-rep5-ITS: 51570
O240-5-rep6-ITS: 51510
BS125-321E-bottom-8-2d-ITS: 51487
F240-4-REP4-ITS: 51356
BS166-240-10cm-9-7d-ITS: 51169
F321-3-rep5-ITS: 51039
BS5-380-10cm-6d-ITS: 50941
BS283-321E-bottom-1d-ITS: 50794
BS292-240-10cm-3-7d-ITS: 50692
BS277-400-bottom-1-5R-ITS: 50445
BS189-400-bottom-2-5R-ITS: 50395
BS284-321E-top-4-4u-ITS: 50313
BS188-400-top-2-5R-ITS: 50255
BS88-321E-top-8-7d-ITS: 50224
BS176-321E-top-6-8u-ITS: 50176
O240-2-rep2-ITS: 50173
O240-P1-REP6-ITS: 50050
BS25-321E-top-2u-ITS: 50016
BS288-321W-10cm-1-8d-ITS: 50005
BS337-240-10cm-4-2u-ITS: 49791
F321-p1-rep1-ITS: 49532
BS194-321E-top-7-6d-ITS: 49506
BS181-321W-10cm-0-6d-ITS: 49381
D321-5-rep2-ITS: 49366
F321-3-rep2-ITS: 49153
BS196-321E-top-5-3u-ITS: 49103
BS29-321E-top-1-5u-ITS: 49063
F240-20-rep1-ITS: 49049
BS98-240-10cm-2-6u-ITS: 49040
BS96-321W-10cm-1-1u-ITS: 49001
BS70-321W-10cm-4-6d-ITS: 48990
BS195-321E-bottom-7-6d-ITS: 48941
BS97-240-10cm-5-3d-ITS: 48919
BS137-400-bottom-1-6L-ITS: 48844
F321-3-rep6-ITS: 48774
BS282-321E-top-1d-ITS: 48722
D240-1-rep5-ITS: 48540
BS228-321E-top-7-5d-ITS: 48531
BS233-321E-bottom-8-8u-ITS: 48485
D240-3-rep6-ITS: 48462
BS12-321W-10cm-9d-ITS: 48419
BS103-321E-bottom-7-6d-ITS: 48417
BS47-321W-10cm-3-5u-ITS: 48386
BS215-321E-bottom-8-4u-ITS: 48385
O240-p2-rep5-ITS: 48289
BS119-240-10cm-3-25d-ITS: 48174
BS31-321W-10cm-8-8u-ITS: 48093
F321-5-rep3-ITS: 48085
O321-3-rep1-ITS: 48041
F321-5-rep6-ITS: 48031
BS72-321W-10cm-5-6u-ITS: 48002
BS39-240-10cm-9-8u-ITS: 47893
D321-1-rep1-ITS: 47873
BS275-240-10cm-10u-ITS: 47810
BS46-321W-10cm-0d-ITS: 47796
BS95-321W-10cm-0-2u-ITS: 47789
O321-1-rep1-ITS: 47671
BS342-400-bottom-7-1L-ITS: 47534
F321-1-rep6-ITS: 47483
BS7-321E-10cm-3d-ITS: 47457
BS85-240-10cm-7-4d-ITS: 47208
BS121-321E-bottom-0-1d-ITS: 47109
BS117-240-10cm-0-55d-ITS: 47107
O321-4-rep6-ITS: 47092
BS159-321E-bottom-4-1d-ITS: 46990
BS104-321E-top-1-9u-ITS: 46981
F321-2-rep2-ITS: 46864
BS126-321W-10cm-3-5d-ITS: 46675
D321-20-rep2-ITS: 46669
BS252-321W-10cm-5-1d-ITS: 46574
BS89-321E-bottom-8-7d-ITS: 46517
F240-5-rep4-ITS: 46487
BS35-240-10cm-3u-ITS: 46430
BS144-321W-10cm-5-9d-ITS: 46411
O321-p1-rep3-ITS: 46325
D240-4-rep4-ITS: 46300
BS248-321E-top-9-6d-ITS: 46260
F321-1-rep3-1-ITS: 46150
BS63-240-10cm-0-4u-ITS: 46092
O321-10-rep5-ITS: 46079
BS42-321E-top-6-6d-ITS: 45970
O240-4-rep1-ITS: 45967
BS302-321E-top-7d-ITS: 45933
BS142-321E-top-4-3u-ITS: 45922
O321-5-rep3-ITS: 45819
F321-p2-rep4-ITS: 45809
D321-10-rep3-ITS: 45756
BS10-321W-10cm-3d-ITS: 45754
BS1-240-10cm-3d-ITS: 45729
F240-4-rep3-ITS: 45599
F321-2-rep1-ITS: 45437
O321-2-rep5-ITS: 45393
F321-10-rep6-ITS: 45342
BS297-400-bottom-4-3R-ITS: 45241
D240-4-rep2-ITS: 45220
BS267-321E-bottom-6-6u-ITS: 45172
F321-p1-rep6-ITS: 45144
D321-3-rep2-ITS: 45115
BS124-321E-top-8-2d-ITS: 45089
D321-1-rep3-ITS: 44941
BS268-321E-top-7-1u-ITS: 44899
D240-10-rep6-ITS: 44865
BS274-240-10cm-6-2u-ITS: 44749
BS99-240-10cm-4-7u-ITS: 44706
D321-2-rep4-ITS: 44700
D321-5-rep3-ITS: 44512
D240-2-rep1-ITS: 44401
D321-2-rep5-ITS: 44346
BS321-321W-bottom-5u-ITS: 44317
BS61-240-10cm-1-6u-ITS: 44302
D240-10-rep5-ITS: 44220
BS14-380-bottom-2u-ITS: 44073
O321-p1-rep5-ITS: 44064
BS132-400-top-8-2L-ITS: 43968
F240-1-rep6-ITS: 43901
BS101-321E-bottom-6-1d-ITS: 43845
D240-10-rep4-ITS: 43581
BS148-240-10cm-5d-ITS: 43517
BS210-321E-top-2-2u-ITS: 43422
BS230-321E-top-7-5u-ITS: 43413
BS129-240-10cm-5-5d-ITS: 43392
O240-20-rep3-ITS: 43387
BS87-240-10cm-6-3u-ITS: 43308
BS247-321E-bottom-4-5d-ITS: 43302
O240-3-REP3-ITS: 43251
BS86-240-10cm-0-3u-ITS: 43198
BS105-321E-bottom-1-9u-ITS: 43179
D321-2-rep1-ITS: 43075
BS200-321W-10cm-9-1u-ITS: 43055
D321-4-rep1-ITS: 42938
BS264-321E-top-6-4u-ITS: 42909
BS182-321W-10cm-4-9d-ITS: 42751
BS131-240-10cm-5-8u-ITS: 42741
BS253-321W-10cm-6-9d-ITS: 42733
BS92-321E-top-4-4u-ITS: 42581
BS220-240-10cm-8-4d-ITS: 42548
BS28-321E-bottom-1u-ITS: 42492
BS102-321E-top-7-6d-ITS: 42491
BS237-240-10cm-1-5u-ITS: 42447
BS44-321E-top-9-3d-ITS: 42429
BS246-321E-top-4-5d-ITS: 42402
KCP1-ITS: 42254
BS130-240-10cm-4u-ITS: 42107
BS301-321E-bottom-5-8d-ITS: 42097
BS243-400-bottom-3-1R-ITS: 42092
BS40-321E-top-6-1u-ITS: 42048
BS211-321E-bottom-2-2u-ITS: 42023
BS308-321W-10cm-6u-ITS: 41935
BS260-400-top-7-3R-ITS: 41884
F321-20-rep1-ITS: 41845
BS193-321E-bottom-2-7d-ITS: 41820
BS120-321E-top-0-1d-ITS: 41794
BS205-400-bottom-7-8R-ITS: 41678
BS170-400-top-6-8R-ITS: 41592
F321-p2-rep6-ITS: 41590
BS273-240-10cm-1-3u-ITS: 41581
BS158-321E-top-4-1d-ITS: 41520
D240-4-rep6-ITS: 41453
F321-3-rep4-ITS: 41364
BS134-400-top-5-5R-ITS: 41327
BS38-240-10cm-2-5u-ITS: 41326
BS290-321W-10cm-9-5d-ITS: 41310
BS23-321E-top-9u-ITS: 41237
BS122-321E-top-0-2d-ITS: 41217
BS108-321W-10cm-3-3u-ITS: 40960
BS16-380-bottom-6u-ITS: 40930
BS13-380-top-2u-ITS: 40891
BS174-321E-top-5-5u-ITS: 40830
BS149-240-10cm-8-6d-ITS: 40703
O240-1-rep4-ITS: 40612
D240-5-rep5-ITS: 40584
O321-3-rep2-ITS: 40489
D240-2-rep6-ITS: 40407
BS152-400-top-10L-ITS: 40400
F321-5-rep4-ITS: 40397
O321-10-rep3-ITS: 40382
BS315-400-bottom-9-7L-ITS: 40308
BS306-321W-10cm-7-9d-ITS: 40250
BS300-321E-top-5-8d-ITS: 40239
O240-20-rep5-ITS: 40066
F321-p2-rep2-ITS: 39966
BS227-400-bottom-1-7L-ITS: 39884
BS115-400-bottom-0-6L-ITS: 39860
F240-4-rep6-ITS: 39846
O321-p2-rep6-ITS: 39844
BS332-321W10m-top-2-9u-ITS: 39789
BS218-321W-10cm-8-3u-ITS: 39751
BS179-321E-bottom-9-4u-ITS: 39743
BS198-321W-10cm-2-9d-ITS: 39681
BS187-400-bottom-3-4R-ITS: 39654
BS107-321W-10cm-5-2d-ITS: 39617
O240-1-rep5-ITS: 39605
O321-3-rep5-ITS: 39580
O321-4-rep3-ITS: 39551
BS65-321E-bottom-2d-ITS: 39533
D321-1-rep4-ITS: 39477
O321-10-rep4-ITS: 39467
BS18-380-bottom-9u-ITS: 39428
BS175-321E-bottom-5-5u-ITS: 39418
BS154-400-top-9-9L-ITS: 39247
BS173-400-bottom-8-4R-ITS: 39210
BS223-400-bottom-0-4L-ITS: 39203
D240-5-rep1-ITS: 39173
BS291-240-10cm-3-3d-ITS: 39111
BS231-321E-bottom-7-5u-ITS: 39096
D321-5-rep4-ITS: 39054
BS201-240-10cm-0-9d-ITS: 39020
BS305-321E-bottom-7-2d-ITS: 38987
O240-10-rep1-ITS: 38971
F240-p1-rep2-ITS: 38954
BS177-321E-bottom-6-8u-ITS: 38869
BS171-400-bottom-6-8R-ITS: 38834
BS143-321E-bottom-4-3u-ITS: 38777
BS106-321W-10cm-1-7d-ITS: 38706
BS295-400-bottom-9R-ITS: 38688
O321-20-rep4-ITS: 38600
F240-2-rep4-ITS: 38580
BS64-321E-top-2d-ITS: 38551
F240-5-rep1-ITS: 38535
BS155-400-bottom-9-9L-ITS: 38445
BS313-400-bottom-7-2L-ITS: 38415
BS91-321E-bottom-4-7u-ITS: 38246
BS276-400-top-1-5R-ITS: 38213
BS229-321E-bottom-7-5d-ITS: 38208
D321-5-rep5-ITS: 38100
BS151-400-bottom-3R-ITS: 38085
D321-20-rep1-ITS: 38017
BS317-400-bottom-9-1L-ITS: 38010
F321-10-rep3-ITS: 37956
O321-p1-rep2-ITS: 37930
BS335-321W10m-bottom-3-8u-ITS: 37922
BS93-321E-bottom-4-4u-ITS: 37912
D240-10-rep3-ITS: 37871
F321-2-rep5-ITS: 37851
O240-5-rep1-ITS: 37834
BS314-400-top-9-7L-ITS: 37815
BS160-321E-top-4-9u-ITS: 37800
O240-4-rep2-ITS: 37756
F321-5-rep1-ITS: 37736
BS136-400-top-1-6L-ITS: 37677
O240-2-rep1-ITS: 37622
BS21-321E-top-6u-ITS: 37622
F321-10-rep1-ITS: 37599
O321-3-rep4-ITS: 37534
F240-p1-rep1-ITS: 37479
BS258-400-top-1-1L-ITS: 37463
F240-p2-rep2-ITS: 37450
BS256-240-10cm-6-5u-ITS: 37427
BS109-400-10cm-rep2-ITS: 37287
BS236-321W-10cm-3-2u-ITS: 37286
D240-3-rep5-ITS: 37149
BS280-400-top-4-3L-ITS: 37146
F321-p1-rep4-ITS: 37138
BS219-240-10cm-4-8d-ITS: 37134
D321-2-rep3-ITS: 37129
BS298-400-top-2-1R-ITS: 37043
BS116-400-bottom-6-3R-ITS: 37003
BS239-240-10cm-9-3u-ITS: 36932
BS37-240-10cm-1d-ITS: 36860
O240-4-rep4-ITS: 36791
BS118-240-10cm-3-05d-ITS: 36731
O321-20-rep3-ITS: 36696
BS209-400-bottom-7-3L-ITS: 36586
BS168-400-top-6-2R-ITS: 36532
O321-2-rep6-ITS: 36527
BS281-400-bottom-4-3L-ITS: 36491
F240-1-rep3-ITS: 36450
F240-4-rep1-ITS: 36372
F321-2-rep6-ITS: 36367
b9-ITS: 36351
O240-10-rep4-ITS: 36204
BS8-321E-10cm-6d-ITS: 36167
D321-3-rep6-ITS: 36133
F240-2-rep6-ITS: 36096
D240-20-rep2-ITS: 36064
BS272-321W-10cm-6-9u-ITS: 36051
D240-4-rep5-ITS: 36038
BS257-240-10cm-8-2u-ITS: 36019
D321-4-rep3-ITS: 35982
BS203-240-10cm-1-2u-ITS: 35949
BS269-321E-bottom-7-1u-ITS: 35920
BS207-400-bottom-7-7R-ITS: 35772
BS232-321E-top-8-8u-ITS: 35764
D321-10-rep2-ITS: 35751
b6-ITS: 35718
BS41-321E-bottom-6-1u-ITS: 35562
BS141-321E-bottom-4-1u-ITS: 35459
O321-4-rep1-ITS: 35301
BS309-240-10cm-8-9d-ITS: 35236
BS11-321W-10cm-6d-ITS: 35207
BS123-321E-bottom-0-2d-ITS: 35190
O321-p2-rep4-ITS: 35089
O321-1-rep3-ITS: 35078
BS169-400-bottom-6-2R-ITS: 35068
BS26-321E-bottom-2u-ITS: 35049
BS224-400-top-3-2L-ITS: 35044
D240-10-rep1-ITS: 35031
BS156-321E-top-2-6d-ITS: 34980
BS67-321E-bottom-5-1u-ITS: 34917
D240-3-rep2-ITS: 34836
F321-p1-rep3-ITS: 34789
O321-5-rep2-ITS: 34771
F240-10-rep2-ITS: 34735
D321-5-rep1-ITS: 34650
O240-p2-rep1-ITS: 34586
BS334-321W10m-top-3-8u-ITS: 34575
F321-4-rep4-ITS: 34574
BS263-400-bottom-4-6L-ITS: 34521
O321-5-rep5-ITS: 34497
BS153-400-bottom-10L-ITS: 34494
BS2-240-10cm-6d-ITS: 34485
D240-1-rep6-ITS: 34481
O321-2-rep3-ITS: 34404
F240-p1-rep4-ITS: 34380
BS322-321W-top-7-4u-ITS: 34367
BS216-321W-10cm-5-6d-ITS: 34349
BS320-321W-top-5u-ITS: 34326
D240-4-rep1-ITS: 34198
b21-ITS: 34179
BS312-400-top-7-2L-ITS: 34134
BS328-321E-top-1-7u-ITS: 34082
BS33-321W-10cm-0-7u-ITS: 34021
BS286-321E-top-6-7u-ITS: 33968
BS287-321E-bottom-6-7u-ITS: 33959
F240-p1-rep3-ITS: 33889
BS299-400-bottom-2-1R-ITS: 33860
F240-1-rep4-ITS: 33832
BS222-400-top-0-4L-ITS: 33820
BS183-240-10cm-0-7d-ITS: 33797
BS324-321E-top-0-1u-ITS: 33796
D240-3-rep1-ITS: 33764
BS245-400-bottom-3-9R-ITS: 33762
D240-20-rep6-ITS: 33676
O240-10-rep2-ITS: 33601
BS163-321W-10cm-6-8d-ITS: 33595
D321-20-rep5-ITS: 33589
BS225-400-bottom-3-2L-ITS: 33585
BS22-321E-bottom-6u-ITS: 33572
BS4-380-10cm-3d-ITS: 33549
BS43-321E-bottom-6-6d-ITS: 33538
O240-p2-rep4-ITS: 33499
D321-20-rep3-ITS: 33379
BS128-321W-10cm-7-9u-ITS: 33330
BS66-321E-top-5-1u-ITS: 33295
D240-3-rep3-ITS: 33273
O321-p1-rep4-ITS: 33198
F240-20-rep6-ITS: 33110
BS190-400-top-6-6L-ITS: 33063
D240-5-rep2-ITS: 33012
F321-p2-rep1-ITS: 32999
BS36-240-10cm-7u-ITS: 32926
BS3-240-10cm-9d-ITS: 32707
BS265-321E-bottom-6-4u-ITS: 32598
BS325-321E-bottom-0-1u-ITS: 32579
BS100-321E-top-6-1d-ITS: 32434
O240-20-rep4-ITS: 32372
BS259-400-bottom-1-1L-ITS: 32275
D240-2-rep5-ITS: 32047
BS90-321E-top-4-7u-ITS: 32044
BS178-321E-top-9-4u-ITS: 31932
BS336-240-10cm-3-9u-ITS: 31821
BS212-321E-top-5-9u-ITS: 31819
F240-20-rep5-ITS: 31592
BS206-400-top-7-7R-ITS: 31343
BS251-321E-bottom-7-8u-ITS: 31292
BS191-400-bottom-6-6L-ITS: 31233
F240-4-rep2-ITS: 31193
BS34-240-10cm-8-4u-ITS: 31007
O321-5-rep6-ITS: 30719
O240-5-rep3-ITS: 30698
BS49-240-10cm-0-4u-ITS: 30553
F240-3-rep4-ITS: 30316
BS114-400-top-4-4L-ITS: 30281
D240-2-rep4-ITS: 30245
BS202-240-10cm-9-8d-ITS: 30173
BS208-400-top-7-3L-ITS: 30115
O240-4-rep6-ITS: 30104
BS270-321W-10cm-1-2d-ITS: 30006
BS68-321E-top-7-3u-ITS: 29997
BS343-400-top-5-9L-ITS: 29874
D321-2-rep2-ITS: 29748
O321-3-rep6-ITS: 29728
b8-ITS: 29728
F321-p2-rep5-ITS: 29701
D240-2-rep3-ITS: 29666
BS316-400-top-9-1L-ITS: 29660
BS249-321E-bottom-9-6d-ITS: 29543
BS235-321W-10cm-5-4d-ITS: 29493
O240-4-rep5-ITS: 29292
BS111-400-top-0-6L-ITS: 29291
BS261-400-bottom-7-3R-ITS: 28997
BS48-321W-10cm-8d-ITS: 28954
O240-20-rep1-ITS: 28914
b23-ITS: 28799
BS240-400-top-0-5L-ITS: 28506
BS167-240-10cm-3-6u-ITS: 28422
F240-2-rep1-ITS: 28331
F321-4-rep3-ITS: 28003
BS304-321E-top-7-2d-ITS: 27981
F240-p2-rep1-ITS: 27920
BS279-400-bottom-9-3R-ITS: 27837
F321-4-rep6-ITS: 27826
F321-1-rep1-ITS: 27726
O240-5-rep2-ITS: 27569
BS172-400-top-8-4R-ITS: 27475
O240-20-rep2-ITS: 27327
BS311-240-10cm-9-9d-ITS: 27303
BS9-321E-10cm-9d-ITS: 27232
D240-2-rep2-ITS: 27071
F321-p1-rep2-ITS: 26993
BS338-240-10cm-4-6u-ITS: 26898
F240-2-rep2-ITS: 26886
BS255-240-10cm-7-8d-ITS: 26865
BS186-400-top-3-4R-ITS: 26762
O240-1-rep6-ITS: 26744
F240-3-rep2-ITS: 26733
F240-1-rep5-ITS: 26519
F240-4-rep5-ITS: 26342
F240-5-rep2-ITS: 26325
F321-20-rep2-ITS: 26320
BS241-400-bottom-0-5L-ITS: 26084
BS296-400-top-4-3R-ITS: 26032
BS340-400-bottom-5L-ITS: 25968
O321-10-rep1-ITS: 25805
BS331-321W10m-bottom-2-3u-ITS: 25706
BS221-240-10cm-1-4u-ITS: 25496
BS244-400-top-3-9R-ITS: 25478
BS45-321E-bottom-9-3d-ITS: 25465
F321-1-rep3-2-ITS: 25358
BS278-400-top-9-3R-ITS: 25351
BS262-400-top-4-6L-ITS: 25307
BS145-321W-10cm-6-2d-ITS: 25299
BS162-321W-10cm-3-1d-ITS: 25101
O240-4-rep3-ITS: 25088
F240-20-rep2-ITS: 25015
BS135-400-bottom-5-5R-ITS: 24936
F240-1-rep2-ITS: 24915
F321-10-rep5-ITS: 24761
F321-20-rep5-ITS: 24551
KCP4-ITS: 24427
BS327-321E-bottom-0-5u-ITS: 24091
BS133-400-bottom-8-2L-ITS: 23996
BS6-380-10cm-9d-ITS: 23801
O240-p2-rep3-ITS: 23758
D240-1-rep4-ITS: 23724
BS112-400-top-6-3R-ITS: 23645
BS113-400-bottom-4-4L-ITS: 23643
BS344-400-bottom-5-9L-ITS: 23558
BS226-400-top-1-7L-ITS: 23303
F321-20-rep4-ITS: 23302
D240-5-rep3-ITS: 23251
O321-20-rep6-ITS: 22990
b7-ITS: 22473
D240-3-rep4-ITS: 22432
D240-4-rep3-ITS: 22177
BS185-240-10cm-5-4u-ITS: 22100
F321-1-rep2-ITS: 21621
D240-5-rep4-ITS: 21364
D240-5-rep6-ITS: 20948
O321-5-rep1-ITS: 20136
BS140-321E-top-4-1u-ITS: 19809
BS294-400-top-9R-ITS: 19588
D240-20-rep4-ITS: 19500
O240-2-rep3-ITS: 19284
O240-1-rep1-ITS: 19172
O240-1-rep3-ITS: 19019
BS150-400-top-3R-ITS: 18431
KCP7-ITS: 18359
b12-ITS: 16511
BS242-400-top-3-1R-ITS: 15690
KCN2-ITS: 15274
b31-ITS: 13870
KCP2-ITS: 10906
b19-ITS: 10501
b18-ITS: 8738
b15-ITS: 8729
b16-ITS: 8506
KCN4-ITS: 8442
b29-ITS: 8261
KCP6-ITS: 7138
b10-ITS: 6888
b17-ITS: 6820
b14-ITS: 6709
KCP5-ITS: 6633
b11-ITS: 6291
KCN3-ITS: 5873
b27-ITS: 4894
KCN1-ITS: 4831
KCP3-ITS: 4809
b25-ITS: 4476
b13-ITS: 4164
b26-ITS: 1603
b28-ITS: 863
b22-ITS: 737
b24-ITS: 666
b30-ITS: 630
b20-ITS: 537
b4-ITS: 21
O240-1-rep2-ITS: 17
b5-ITS: 16
b1-ITS: 8
O240-3-rep1-ITS: 7
b2-ITS: 5
b3-ITS: 4
[Jan 06 03:56 PM]: Output file: allITS_AMPTKpreprocessed.demux.fq.gz (1.5 GB)
[Jan 06 03:56 PM]: Mapping file: allITS_AMPTKpreprocessed.mapping_file.txt
-------------------------------------------------------