Wellcome Connecting Science Course Website Link
Course Time Table 2024
CRISPR/Cas-based and related gene editing technologies have made it possible for many laboratories to perform genome editing with relative ease. These tools have helped to provide valuable information regarding genetic interactions and in the advancement of gene therapy in precision medicine.
This new suite of virtual and lab-based training courses offers participants the opportunity to delve deeper into the complexities of genetic engineering by providing a comprehensive overview of the theory and practical laboratory skills required for the genetic manipulation of mammalian cells for cellular modelling and screening. The courses will each be focused on a particular learning outcome: (1) Genome Informatics and Computational Design and Analysis (2) Cellular Generation and Modelling and (3) Pooled and Arrayed Genetic Screening. Each course will consist of interactive practicals, demonstrations, discussion sessions, and lectures by international experts.
This course is targeted at postdoctoral scientists, advanced PhD students actively engaged in or soon to commence relevant research. It is also suitable for facility managers and principal investigators wishing to bring the latest genetic modification technologies into their facilities or groups.
The courses will cover topics including:
CRISPR: Genome Informatics and Computational Design and Analysis
- Fundamentals of CRISPR
- Experimental and library design
- Gene editing
- Therapeutic genome editing
- Quality control for generated cellular models
- CRISPR: Cellular Generation and Modelling
IPSC culture and cloning
- Gene editing
- Genotype and clone characterisation
- Differentiation
- CRISPR: Pooled and Arrayed Genetic Screening
Library generation
- High-throughput screening arrays
- High-throughput editing efficiency
- Pooled screening
CRISPR: Genome Informatics and Computational Design and Analysis
- Design genetic engineering strategies for generating cellular models, including choice of different methodologies (HDR, KO, conditional, prime or base editing) and validation strategies
- Gain an in depth understanding of CRISPR-based genetic screening methods, including design, reagents and techniques including their advantages and limitations
CRISPR: Cellular Generation and Modelling
- Undertake genome editing and cellular modelling with diverse cellular models, including manipulation of cells, generation and delivery of reagents, and isolation and analysis of clones.
CRISPR: Pooled and Arrayed Genetic Screening
- Design and execute CRISPR based genetic screening approaches in both pooled and arrayed formats including experimental design and analysis.
Course Instructors
- Matthew Coelho, Wellcome Sanger Institute, UK
- Kalpana Surendranath, University of Westminster, UK
- Andrew Bassett, Wellcome Sanger Institute, UK
- Nneka Anyanwu, Wellcome Sanger Institute, UK
- Panos Zalmas, Open Targets, UK
Wellcome Connecting Science Team
- Jorge Batista da Rocha, Education Developer
- Cassandra Soo, Laboratory Courses Manager
- Aaron Dean, Laboratory Technical Officer
- Amy Pass, Events Organiser
- Martin Aslett, Informatics Manager
- Vaishnavi Vikas Gangadhar, Informatics Technical Officer
- Alice Matimba, Head of Training and Global Capacity
- Michelle Bishop, Associate Director
The course data are free to reuse and adapt with appropriate attribution. All course data in these repositories are licensed under the Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0).
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