From 6de1b92c3ba7d415abb3166d17a8bcdc8c553a9f Mon Sep 17 00:00:00 2001 From: Ahsan Date: Tue, 15 Dec 2020 12:07:18 -0500 Subject: [PATCH] fixed a bug with closing multiprocessing pool --- sample/log | 118 +++++++------ sample/sample_call | 2 +- .../test_run/HG002.chr22.sample.final.vcf.gz | Bin 41156 -> 41145 bytes .../HG002.chr22.sample.final.vcf.gz.tbi | Bin 675 -> 678 bytes ...G002.chr22.sample.indels.decomposed.vcf.gz | Bin 16305 -> 16294 bytes ....chr22.sample.indels.decomposed.vcf.gz.tbi | Bin 648 -> 649 bytes sample/test_run/HG002.chr22.sample.indels.vcf | 22 +-- .../test_run/HG002.chr22.sample.indels.vcf.gz | Bin 13180 -> 13187 bytes .../HG002.chr22.sample.indels.vcf.gz.tbi | Bin 658 -> 661 bytes .../HG002.chr22.sample.snps.phased.bam.bai | Bin 51992 -> 51992 bytes ...G002.chr22.sample.snps.phased.preclean.vcf | 2 +- .../HG002.chr22.sample.snps.phased.vcf.gz | Bin 26014 -> 26011 bytes .../HG002.chr22.sample.snps.phased.vcf.gz.tbi | Bin 672 -> 671 bytes sample/test_run/HG002.chr22.sample.snps.vcf | 2 +- .../test_run/HG002.chr22.sample.snps.vcf.gz | Bin 24995 -> 24995 bytes sample/test_run/args | 2 +- sample/test_run/ref.sdf/done | 2 +- sample/test_run/ref.sdf/format.log | 155 +++++++++--------- sample/test_run/ref.sdf/mainIndex | Bin 24304 -> 24304 bytes sample/test_run/ref.sdf/progress | 6 +- sample/test_run/ref.sdf/summary.txt | 2 +- scripts/NanoCaller.py | 5 +- 22 files changed, 168 insertions(+), 150 deletions(-) diff --git a/sample/log b/sample/log index e8e11f8..237d4ae 100644 --- a/sample/log +++ b/sample/log @@ -1,23 +1,28 @@ -2020-11-03 12:54:37.546552: Starting NanoCaller. +2020-12-15 11:51:46.510937: Starting NanoCaller. Running arguments are saved in the following file: test_run/args -2020-11-03 12:54:44.877308: SNP calling started. -2020-11-03 12:54:46.708984: Coverage=49.63x. -2020-11-03 12:55:07.620834: (1/5) regions completed. -2020-11-03 12:55:21.178505: (2/5) regions completed. -2020-11-03 12:55:32.449525: (3/5) regions completed. -2020-11-03 12:55:44.149472: (4/5) regions completed. -2020-11-03 12:55:55.296145: (5/5) regions completed. +2020-12-15 11:51:54.445175: SNP calling started. -2020-11-03 12:55:55.611312: SNP calling completed for contig chr22. Time taken= 70.7340 +2020-12-15 11:51:56.552637: Coverage=49.63x. +2020-12-15 11:52:20.333301: (1/5) regions completed. +2020-12-15 11:52:36.487862: (2/5) regions completed. +2020-12-15 11:52:49.490300: (3/5) regions completed. +2020-12-15 11:53:02.292401: (4/5) regions completed. +2020-12-15 11:53:14.600400: (5/5) regions completed. +Writing to /tmp/bcftools-sort.fnHPHD +Merging 1 temporary files +Cleaning +Done -2020-11-03 12:55:55.611387: ------WhatsHap SNP phasing log------ +2020-12-15 11:53:14.967118: SNP calling completed for contig chr22. Time taken= 80.5220 -This is WhatsHap 1.0 running under Python 3.6.8 +2020-12-15 11:53:14.967204: ------WhatsHap SNP phasing log------ + +This is WhatsHap 1.0 running under Python 3.6.11 Working on 1 samples from 1 family ======== Working on chromosome 'chr22' ---- Processing individual SAMPLE @@ -40,22 +45,23 @@ Largest component contains 1348 variants (100.0% of accessible variants) between Done writing VCF == SUMMARY == -Maximum memory usage: 0.699 GB -Time spent reading BAM/CRAM: 6.2 s +Maximum memory usage: 0.355 GB +Time spent reading BAM/CRAM: 5.0 s Time spent parsing VCF: 0.0 s -Time spent selecting reads: 0.3 s -Time spent phasing: 5.8 s -Time spent writing VCF: 0.0 s +Time spent selecting reads: 0.5 s +Time spent phasing: 8.1 s +Time spent writing VCF: 0.1 s Time spent finding components: 0.0 s -Time spent on rest: 0.3 s -Total elapsed time: 12.7 s +Time spent on rest: 0.4 s +Total elapsed time: 14.0 s + -2020-11-03 12:56:11.768244: ------SNP phasing completed------ +2020-12-15 11:53:33.743588: ------SNP phasing completed------ -2020-11-03 12:56:11.768306: ------WhatsHap BAM phasing log------ +2020-12-15 11:53:33.743669: ------WhatsHap BAM phasing log------ Found 1 samples in input VCF Keeping 1 samples for haplo-tagging @@ -67,33 +73,45 @@ Found 2474 reads covering 1323 variants Total alignments processed: 3967 Alignments that could be tagged: 2612 Alignments spanning multiple phase sets: 0 -haplotag - total processing time: 8.877756118774414 - - -2020-11-03 12:56:23.551385: ------BAM phasing completed----- - -2020-11-03 12:56:23.551511: Indel calling started. -2020-11-03 12:56:37.223048: (1/20) regions completed. -2020-11-03 12:56:49.573645: (2/20) regions completed. -2020-11-03 12:57:08.137466: (3/20) regions completed. -2020-11-03 12:57:30.306719: (4/20) regions completed. -2020-11-03 12:57:46.430516: (5/20) regions completed. -2020-11-03 12:58:01.844967: (6/20) regions completed. -2020-11-03 12:58:11.831042: (7/20) regions completed. -2020-11-03 12:58:33.912745: (8/20) regions completed. -2020-11-03 12:58:52.015400: (9/20) regions completed. -2020-11-03 12:59:06.197186: (10/20) regions completed. -2020-11-03 12:59:24.105673: (11/20) regions completed. -2020-11-03 13:01:08.098350: (12/20) regions completed. -2020-11-03 13:01:19.541288: (13/20) regions completed. -2020-11-03 13:01:33.742843: (14/20) regions completed. -2020-11-03 13:01:46.493032: (15/20) regions completed. -2020-11-03 13:02:00.343629: (16/20) regions completed. -2020-11-03 13:02:15.169278: (17/20) regions completed. -2020-11-03 13:02:29.679240: (18/20) regions completed. -2020-11-03 13:02:47.559419: (19/20) regions completed. -2020-11-03 13:03:06.198732: (20/20) regions completed. -2020-11-03 13:03:06.488109: Indel calling completed for contig chr22. Time taken= 402.9366 -2020-11-03 13:03:06.488197: Post processing - -2020-11-03 13:03:11.648681: Total Time Elapsed: 514.12 seconds +haplotag - total processing time: 7.949145078659058 + + + + +2020-12-15 11:53:45.776510: ------BAM phasing completed----- + +2020-12-15 11:53:45.776640: Indel calling started. +2020-12-15 11:54:05.073745: (1/20) regions completed. +2020-12-15 11:54:23.467969: (2/20) regions completed. +2020-12-15 11:54:50.175749: (3/20) regions completed. +2020-12-15 11:55:22.075120: (4/20) regions completed. +2020-12-15 11:55:46.103592: (5/20) regions completed. +2020-12-15 11:56:09.731025: (6/20) regions completed. +2020-12-15 11:56:24.271868: (7/20) regions completed. +2020-12-15 11:56:58.973673: (8/20) regions completed. +2020-12-15 11:57:27.175183: (9/20) regions completed. +2020-12-15 11:57:48.967288: (10/20) regions completed. +2020-12-15 11:58:15.602348: (11/20) regions completed. +2020-12-15 12:00:44.880064: (12/20) regions completed. +2020-12-15 12:01:03.013816: (13/20) regions completed. +2020-12-15 12:01:24.416389: (14/20) regions completed. +2020-12-15 12:01:44.582849: (15/20) regions completed. +2020-12-15 12:02:05.310054: (16/20) regions completed. +2020-12-15 12:02:27.753123: (17/20) regions completed. +2020-12-15 12:02:50.474625: (18/20) regions completed. +2020-12-15 12:03:18.572774: (19/20) regions completed. +2020-12-15 12:03:46.566836: (20/20) regions completed. +Writing to /tmp/bcftools-sort.oKHcDK +Merging 1 temporary files +Cleaning +Done + +2020-12-15 12:03:46.930933: Indel calling completed for contig chr22. Time taken= 601.1544 +2020-12-15 12:03:46.973526: Post processing + + + +Checking the headers and starting positions of 2 files + + +2020-12-15 12:03:54.094108: Total Time Elapsed: 727.59 seconds diff --git a/sample/sample_call b/sample/sample_call index 8dc8e06..3c1760c 100644 --- a/sample/sample_call +++ b/sample/sample_call @@ -1 +1 @@ -python ../scripts/NanoCaller.py -bam HG002.nanopore.chr22.sample.bam -mode both -seq ont -model NanoCaller1 -vcf test_run -chrom chr22 -start 20000000 -end 21000000 -ref chr22_ref.fa -prefix HG002.chr22.sample -cpu 1 > log +python ../scripts/NanoCaller.py -bam HG002.nanopore.chr22.sample.bam -mode both -seq ont -model NanoCaller1 -o test_run -chrom chr22 -start 20000000 -end 21000000 -ref chr22_ref.fa -prefix HG002.chr22.sample -cpu 1 > log diff --git a/sample/test_run/HG002.chr22.sample.final.vcf.gz b/sample/test_run/HG002.chr22.sample.final.vcf.gz index 9ea8d1aa1d3652acaa0461065b348790e84da1c0..3073b4d8490bc2467fd2e04025391118a39bac76 100644 GIT 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zcmV;_04x8a!vUki0kAeVe`3^u4Jd}y&?wy(6Pd6^9FUJIYDx8PE$OZUbDU*4?BS?| zu8_Nx4r`D@{4uN#{$9vKmIc|xr-gf974?q^cDi=Ffzol4(x}|XIO%RJ;At(0cmdiF z|70W9#1dJEXzD3yvjg;h2LatVq#m;eDf3LMtrhKMSxe*zI$2(nMfOR?)v|%2v|0Md zSsldv)OK6BF(V5-t8^0Q;j O{{libUQo08H>oN1vu%9< diff --git a/sample/test_run/args b/sample/test_run/args index c8fc352..109b874 100644 --- a/sample/test_run/args +++ b/sample/test_run/args @@ -1 +1 @@ -Namespace(bam='HG002.nanopore.chr22.sample.bam', chrom='chr22', cpu=1, del_threshold=0.6, enable_whatshap=False, end=21000000, exclude_bed=None, include_bed=None, ins_threshold=0.4, maxcov=160, min_allele_freq=0.15, min_nbr_sites=1, mincov=8, mode='both', model='NanoCaller1', neighbor_threshold='0.4,0.6', prefix='HG002.chr22.sample', ref='chr22_ref.fa', sample='SAMPLE', sequencing='ont', start=20000000, supplementary=False, vcf='test_run', wgs_contigs_type='with_chr', wgs_print_commands=False) \ No newline at end of file +Namespace(bam='HG002.nanopore.chr22.sample.bam', chrom='chr22', cpu=1, del_threshold=0.6, enable_whatshap=False, end=21000000, exclude_bed=None, include_bed=None, ins_threshold=0.4, keep_bam=False, maxcov=160, min_allele_freq=0.15, min_nbr_sites=1, mincov=8, mode='both', model='NanoCaller1', neighbor_threshold='0.4,0.6', output='test_run', prefix='HG002.chr22.sample', ref='chr22_ref.fa', sample='SAMPLE', sequencing='ont', start=20000000, supplementary=False) \ No newline at end of file diff --git a/sample/test_run/ref.sdf/done b/sample/test_run/ref.sdf/done index f310827..2cb0e09 100644 --- a/sample/test_run/ref.sdf/done +++ b/sample/test_run/ref.sdf/done @@ -1 +1 @@ -Finished successfully in 1 s. +Finished successfully in 2 s. diff --git a/sample/test_run/ref.sdf/format.log b/sample/test_run/ref.sdf/format.log index d09fce7..1f78a89 100644 --- a/sample/test_run/ref.sdf/format.log +++ b/sample/test_run/ref.sdf/format.log @@ -1,79 +1,78 @@ -2020-11-03 13:03:08 serial = 27182818284 -2020-11-03 13:03:08 email = anon -2020-11-03 13:03:08 RTG version = RTG Tools 3.11 / Core 6e08b60e18 (2020-02-26) -2020-11-03 13:03:08 awt.toolkit = sun.awt.X11.XToolkit -2020-11-03 13:03:08 file.encoding = UTF-8 -2020-11-03 13:03:08 file.separator = / -2020-11-03 13:03:08 host.name = c-2-02 -2020-11-03 13:03:08 java.awt.graphicsenv = sun.awt.X11GraphicsEnvironment -2020-11-03 13:03:08 java.awt.printerjob = sun.print.PSPrinterJob -2020-11-03 13:03:08 java.class.path = /home/ahsanm1/anaconda3/envs/new_tf/share/rtg-tools-3.11-0/RTG.jar -2020-11-03 13:03:08 java.class.version = 55.0 -2020-11-03 13:03:08 java.home = /home/ahsanm1/anaconda3/envs/new_tf -2020-11-03 13:03:08 java.io.tmpdir = /tmp -2020-11-03 13:03:08 java.library.path = /home/ahsanm1/anaconda3/envs/new_tf/share/rtg-tools-3.11-0 -2020-11-03 13:03:08 java.runtime.name = OpenJDK Runtime Environment -2020-11-03 13:03:08 java.runtime.version = 11.0.1+13-LTS -2020-11-03 13:03:08 java.specification.name = Java Platform API Specification -2020-11-03 13:03:08 java.specification.vendor = Oracle Corporation -2020-11-03 13:03:08 java.specification.version = 11 -2020-11-03 13:03:08 java.vendor = Azul Systems, Inc. -2020-11-03 13:03:08 java.vendor.url = http://www.azulsystems.com/ -2020-11-03 13:03:08 java.vendor.url.bug = http://www.azulsystems.com/support/ -2020-11-03 13:03:08 java.vendor.version = Zulu11.2+3 -2020-11-03 13:03:08 java.version = 11.0.1 -2020-11-03 13:03:08 java.version.date = 2018-10-16 -2020-11-03 13:03:08 java.vm.compressedOopsMode = Zero based -2020-11-03 13:03:08 java.vm.info = mixed mode -2020-11-03 13:03:08 java.vm.name = OpenJDK 64-Bit Server VM -2020-11-03 13:03:08 java.vm.specification.name = Java Virtual Machine Specification -2020-11-03 13:03:08 java.vm.specification.vendor = Oracle Corporation -2020-11-03 13:03:08 java.vm.specification.version = 11 -2020-11-03 13:03:08 java.vm.vendor = Azul Systems, Inc. -2020-11-03 13:03:08 java.vm.version = 11.0.1+13-LTS -2020-11-03 13:03:08 jdk.debug = release -2020-11-03 13:03:08 lang.version = 11.0.1 -2020-11-03 13:03:08 line.separator = +2020-12-15 12:03:49 serial = 27182818284 +2020-12-15 12:03:49 email = anon +2020-12-15 12:03:49 RTG version = RTG Tools 3.11 / Core 6e08b60e18 (2020-02-26) +2020-12-15 12:03:49 awt.toolkit = sun.awt.X11.XToolkit +2020-12-15 12:03:49 file.encoding = UTF-8 +2020-12-15 12:03:49 file.separator = / +2020-12-15 12:03:49 host.name = reslnvhpc017 +2020-12-15 12:03:49 java.awt.graphicsenv = sun.awt.X11GraphicsEnvironment +2020-12-15 12:03:49 java.awt.printerjob = sun.print.PSPrinterJob +2020-12-15 12:03:49 java.class.path = /home/ahsanm1/anaconda3/envs/NanoCaller/share/rtg-tools-3.11-0/RTG.jar +2020-12-15 12:03:49 java.class.version = 55.0 +2020-12-15 12:03:49 java.home = /home/ahsanm1/anaconda3/envs/NanoCaller +2020-12-15 12:03:49 java.io.tmpdir = /tmp +2020-12-15 12:03:49 java.library.path = /home/ahsanm1/anaconda3/envs/NanoCaller/share/rtg-tools-3.11-0 +2020-12-15 12:03:49 java.runtime.name = OpenJDK Runtime Environment +2020-12-15 12:03:49 java.runtime.version = 11.0.8-internal+0-adhoc..src +2020-12-15 12:03:49 java.specification.name = Java Platform API Specification +2020-12-15 12:03:49 java.specification.vendor = Oracle Corporation +2020-12-15 12:03:49 java.specification.version = 11 +2020-12-15 12:03:49 java.vendor = N/A +2020-12-15 12:03:49 java.vendor.url = https://openjdk.java.net/ +2020-12-15 12:03:49 java.vendor.url.bug = https://bugreport.java.com/bugreport/ +2020-12-15 12:03:49 java.version = 11.0.8-internal +2020-12-15 12:03:49 java.version.date = 2020-07-14 +2020-12-15 12:03:49 java.vm.compressedOopsMode = Zero based +2020-12-15 12:03:49 java.vm.info = mixed mode +2020-12-15 12:03:49 java.vm.name = OpenJDK 64-Bit Server VM +2020-12-15 12:03:49 java.vm.specification.name = Java Virtual Machine Specification +2020-12-15 12:03:49 java.vm.specification.vendor = Oracle Corporation +2020-12-15 12:03:49 java.vm.specification.version = 11 +2020-12-15 12:03:49 java.vm.vendor = Oracle Corporation +2020-12-15 12:03:49 java.vm.version = 11.0.8-internal+0-adhoc..src +2020-12-15 12:03:49 jdk.debug = release +2020-12-15 12:03:49 lang.version = 11.0.8-internal +2020-12-15 12:03:49 line.separator = -2020-11-03 13:03:08 models.dir = /home/ahsanm1/anaconda3/envs/new_tf/share/rtg-tools-3.11-0/models -2020-11-03 13:03:08 nashorn.option.no.deprecation.warning = true -2020-11-03 13:03:08 os.arch = amd64 -2020-11-03 13:03:08 os.freememory = 1382596608 -2020-11-03 13:03:08 os.name = Linux -2020-11-03 13:03:08 os.totalmemory = 134967234560 -2020-11-03 13:03:08 os.version = 3.10.0-1127.18.2.el7.x86_64 -2020-11-03 13:03:08 path.separator = : -2020-11-03 13:03:08 processor.arch = amd64 -2020-11-03 13:03:08 processor.count = 24 -2020-11-03 13:03:08 references.dir = /home/ahsanm1/anaconda3/envs/new_tf/share/rtg-tools-3.11-0/references -2020-11-03 13:03:08 runtime.freememory = 2099249152 -2020-11-03 13:03:08 runtime.maxmemory = 4294967296 -2020-11-03 13:03:08 runtime.totalMemory = 2109734912 -2020-11-03 13:03:08 sun.arch.data.model = 64 -2020-11-03 13:03:08 sun.boot.library.path = /home/ahsanm1/anaconda3/envs/new_tf/lib -2020-11-03 13:03:08 sun.cpu.endian = little -2020-11-03 13:03:08 sun.cpu.isalist = -2020-11-03 13:03:08 sun.io.unicode.encoding = UnicodeLittle -2020-11-03 13:03:08 sun.java.command = /home/ahsanm1/anaconda3/envs/new_tf/share/rtg-tools-3.11-0/RTG.jar format -f fasta test_run/chr22.fa -o test_run/ref.sdf -2020-11-03 13:03:08 sun.java.launcher = SUN_STANDARD -2020-11-03 13:03:08 sun.jnu.encoding = UTF-8 -2020-11-03 13:03:08 sun.management.compiler = HotSpot 64-Bit Tiered Compilers -2020-11-03 13:03:08 sun.os.patch.level = unknown -2020-11-03 13:03:08 talkback = true -2020-11-03 13:03:08 usage = false -2020-11-03 13:03:08 user.country = US -2020-11-03 13:03:08 user.dir = /home/ahsanm1/repos/NanoCaller/sample -2020-11-03 13:03:08 user.home = /home/ahsanm1 -2020-11-03 13:03:08 user.language = en -2020-11-03 13:03:08 user.name = ahsanm1 -2020-11-03 13:03:08 user.timezone = America/New_York -2020-11-03 13:03:08 vendor = Azul Systems, Inc. -2020-11-03 13:03:08 gzipfix.enabled = true -2020-11-03 13:03:08 Command line arguments: rtg format -f fasta test_run/chr22.fa -o test_run/ref.sdf -2020-11-03 13:03:08 Run Id: 453dd090-43ba-429c-a535-2b39d03d5b8d -2020-11-03 13:03:08 Formatting FASTA data -2020-11-03 13:03:08 Performing trimming with no trimming -2020-11-03 13:03:08 Processing "test_run/chr22.fa" -2020-11-03 13:03:09 Writing SDF-Id: 0eaa8bc9-9387-4eb0-a5cb-194d18983f62 -2020-11-03 13:03:09 Referenced SDF-ID: 0eaa8bc9-9387-4eb0-a5cb-194d18983f62 Type: DNA Sequences: 1 Max-Length: 50818468 Min-Length: 50818468 Arm: UNKNOWN -2020-11-03 13:03:09 Finished successfully in 1 s. +2020-12-15 12:03:49 models.dir = /home/ahsanm1/anaconda3/envs/NanoCaller/share/rtg-tools-3.11-0/models +2020-12-15 12:03:49 nashorn.option.no.deprecation.warning = true +2020-12-15 12:03:49 os.arch = amd64 +2020-12-15 12:03:49 os.freememory = 1107992576 +2020-12-15 12:03:49 os.name = Linux +2020-12-15 12:03:49 os.totalmemory = 12429058048 +2020-12-15 12:03:49 os.version = 3.10.0-1160.2.1.el7.x86_64 +2020-12-15 12:03:49 path.separator = : +2020-12-15 12:03:49 processor.arch = amd64 +2020-12-15 12:03:49 processor.count = 4 +2020-12-15 12:03:49 references.dir = /home/ahsanm1/anaconda3/envs/NanoCaller/share/rtg-tools-3.11-0/references +2020-12-15 12:03:49 runtime.freememory = 185763568 +2020-12-15 12:03:49 runtime.maxmemory = 4294967296 +2020-12-15 12:03:49 runtime.totalMemory = 195035136 +2020-12-15 12:03:49 sun.arch.data.model = 64 +2020-12-15 12:03:49 sun.boot.library.path = /home/ahsanm1/anaconda3/envs/NanoCaller/lib +2020-12-15 12:03:49 sun.cpu.endian = little +2020-12-15 12:03:49 sun.cpu.isalist = +2020-12-15 12:03:49 sun.io.unicode.encoding = UnicodeLittle +2020-12-15 12:03:49 sun.java.command = /home/ahsanm1/anaconda3/envs/NanoCaller/share/rtg-tools-3.11-0/RTG.jar format -f fasta test_run/chr22.fa -o test_run/ref.sdf +2020-12-15 12:03:49 sun.java.launcher = SUN_STANDARD +2020-12-15 12:03:49 sun.jnu.encoding = UTF-8 +2020-12-15 12:03:49 sun.management.compiler = HotSpot 64-Bit Tiered Compilers +2020-12-15 12:03:49 sun.os.patch.level = unknown +2020-12-15 12:03:49 talkback = true +2020-12-15 12:03:49 usage = false +2020-12-15 12:03:49 user.country = US +2020-12-15 12:03:49 user.dir = /home/ahsanm1/repos/NanoCaller/sample +2020-12-15 12:03:49 user.home = /home/ahsanm1 +2020-12-15 12:03:49 user.language = en +2020-12-15 12:03:49 user.name = ahsanm1 +2020-12-15 12:03:49 user.timezone = America/New_York +2020-12-15 12:03:49 vendor = N/A +2020-12-15 12:03:49 gzipfix.enabled = true +2020-12-15 12:03:49 Command line arguments: rtg format -f fasta test_run/chr22.fa -o test_run/ref.sdf +2020-12-15 12:03:49 Run Id: db9b0a6e-4e24-487a-a135-7110f88c4a37 +2020-12-15 12:03:49 Formatting FASTA data +2020-12-15 12:03:49 Performing trimming with no trimming +2020-12-15 12:03:49 Processing "test_run/chr22.fa" +2020-12-15 12:03:51 Writing SDF-Id: 61ba592b-2671-4aef-adba-8c977bea1018 +2020-12-15 12:03:51 Referenced SDF-ID: 61ba592b-2671-4aef-adba-8c977bea1018 Type: DNA Sequences: 1 Max-Length: 50818468 Min-Length: 50818468 Arm: UNKNOWN +2020-12-15 12:03:51 Finished successfully in 2 s. diff --git a/sample/test_run/ref.sdf/mainIndex b/sample/test_run/ref.sdf/mainIndex index f1fbec5535e599a87ddccd30728cb52afd1dfb03..5f446b1d82cb08ce467c379c6f2474f70d7d5a2a 100644 GIT binary patch delta 53 zcmV-50LuUHy#esO0kA<{2(7w|mwW0E7?Vj}X%Tj3Td^7d0RaJFx>+kGaZ2x#!(Jr_ L&QO-+J@JvdXjT^J delta 53 zcmV-50LuUHy#esO0kA<{2&KyzO&FLzVv|W;X%TggM&%a(0RaIHs*A~!hfc7Q!(Jr_ LkDqQ6dyKk(P&^gQ diff --git a/sample/test_run/ref.sdf/progress b/sample/test_run/ref.sdf/progress index 20e6958..6592d6e 100644 --- a/sample/test_run/ref.sdf/progress +++ b/sample/test_run/ref.sdf/progress @@ -1,3 +1,3 @@ -2020-11-03 13:03:08 Started -2020-11-03 13:03:08 Formatting FASTA data -2020-11-03 13:03:09 Finished successfully in 1 s. +2020-12-15 12:03:49 Started +2020-12-15 12:03:49 Formatting FASTA data +2020-12-15 12:03:51 Finished successfully in 2 s. diff --git a/sample/test_run/ref.sdf/summary.txt b/sample/test_run/ref.sdf/summary.txt index e1395b6..972eb1a 100644 --- a/sample/test_run/ref.sdf/summary.txt +++ b/sample/test_run/ref.sdf/summary.txt @@ -10,7 +10,7 @@ Mean length : 50818468 Maximum length : 50818468 Output Data -SDF-ID : 0eaa8bc9-9387-4eb0-a5cb-194d18983f62 +SDF-ID : 61ba592b-2671-4aef-adba-8c977bea1018 Number of sequences: 1 Total residues : 50818468 Minimum length : 50818468 diff --git a/scripts/NanoCaller.py b/scripts/NanoCaller.py index c430c8f..cfe5343 100644 --- a/scripts/NanoCaller.py +++ b/scripts/NanoCaller.py @@ -148,8 +148,9 @@ def run(args): final_path=os.path.join(args.output,'%s.final.vcf.gz' %args.prefix) run_cmd('bcftools concat %s.phased.vcf.gz %s.decomposed.vcf.gz -a -d all |bgziptabix %s' %(snp_vcf, indel_vcf, final_path)) - pool.close() - pool.join() + if pool: + pool.close() + pool.join() if __name__ == '__main__':