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Well, it may certainly work, however, it will certainly assemble only separate fragments. |
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Hi, I would like to know if RAD-seq Illumina reads can be de novo assembled on Spades so as to produce contigs that match genes/genome of an organism of interest? There are tools such as Rainbow (https://academic.oup.com/bioinformatics/article/28/21/2732/233132) that seem to be specifically geared to address this but I'm just curious to know if Spades would work correctly if I input RAD-seq Illumina reads to it?
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