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default_parameters.yaml
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default_parameters.yaml
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paths :
bowtie_dir : ''
rsem_dir : ''
python_dir : ''
java_dir : ''
samtools_dir : ''
parameters :
umi_quantification_arguments:
m : 10 #Ignore reads with more than M alignments, after filtering on distance from transcript end.
u : 1 #Ignore counts from UMI that should be split among more than U genes.
d : 600 #Maximal distance from transcript end, NOT INCLUDING THE POLYA TAIL
split-ambigs: False #If umi is assigned to m genes, add 1/m to each gene's count (instead of 1)
min_non_polyA: 15 #Require reads to align to this much non-polyA sequence. (Set to 0 to disable filtering on this parameter.)
output_arguments:
output_unaligned_reads_to_other_fastq: False
filter_alignments_to_softmasked_regions: False
bowtie_arguments:
m : 200
n : 1
l : 15
e : 80
trimmomatic_arguments:
LEADING: "28"
SLIDINGWINDOW: "4:20"
MINLEN: "16"
argument_order: ['LEADING', 'SLIDINGWINDOW', 'MINLEN']
low_complexity_filter_arguments:
max_low_complexity_fraction : 0.50