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Merge pull request #20 from biosustain/upload-articles
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mialpo authored Dec 13, 2024
2 parents ddc59f5 + b1cb630 commit e871371
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8 changes: 7 additions & 1 deletion _static/custom.css
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figure.align-center {
text-align: center;
}
}

.admonition.my-custom-admonition {
background-color: #e8daed;
border-left: 4px solid #ab86ba;
padding: 10px;
}
42 changes: 42 additions & 0 deletions guides/sequence_creation.md
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# Create and import sequences

## Introduction 

One of the first steps to start using Benchling’s Molecular Biology tool is to upload the sequences of interest into the platform. This will allow you to view, annotate and modify the DNA and RNA sequences you need to work with. It is possible to create plasmids, DNA fragments, genes, primers and more, and there are several ways to do this. This guide will cover the most common ones.  

## Get started

Locate the sequence creation menu:

(VIDEO)

**Note**: Always double-check which folder you are saving to!

## Create a new sequence

This can be useful for raw sequences. For example, if you have a .docx file with a sequence, or the PDF of a paper with a sequence you are interested in, you can easily copy and paste it. The menu allows you to set the topology and schema depending on what your sequence corresponds to.  

(VIDEO)

```{admonition} *Try it out!*
:class: my-custom-admonition
➜ Create a file for the **pCAT** promoter (agaggttccaactttcaccataatgaaaca) 
```

## Upload a file

If you have one or more sequences saved to your computer, you can upload them. The sequence, annotations and comments will be imported.  

(Video)

```{admonition} *Try it out!*
:class: my-custom-admonition
➜ Download the file for the **pET-28b(+)** plasmid and upload it to Benchling.
[pET-28b(+)](https://www.snapgene.com/plasmids/pet_and_duet_vectors_(novagen)/pET-28b(%2B))
```

## Import from databases

1 change: 1 addition & 0 deletions index.md
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Overview <guides/introduction>
Register your strains <guides/strain_registration>
Create sequences <guides/sequence_creation.md>
```

```{toctree}
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