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Expand description of registering multiple channels
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adamltyson committed Oct 24, 2023
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Expand Up @@ -31,9 +31,18 @@ e.g. `psl`. For more details on this, see the outline
[here](https://docs.brainglobe.info/cellfinder/image-orientation#orientation). Lastly, set an `Output directory`
(where you want to save the data).

### Registering additional channels
`brainreg` will use a single channel for registration. This is typically an image without much signal,
such as an image of only autofluroescence. Images of brain-wide stains such as DAPI can also work well.

To register any additional channels, make sure these are selected in the list of layers on the left-hand side of the
napari window. The registration will be performed on the image chosen as `Image layer`, but the transformations will be
applied to these other channels. This is useful if you want to later analyse multiple channels, or if the channel of interest
registers poorly due to high signal levels from staining etc.

:::{caution}
Make sure that the image layer you are registering is not selected in the list of napari image layers on the left-hand
side. Any images that are selected will also be registered along with the image layer chosen in `Image layer`.
side, otherwise it will be registered twice!
:::

## Setting additional parameters
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