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added example data and walkthrough
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brandonsie committed Jan 5, 2023
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Expand Up @@ -7,6 +7,9 @@ More specifically, `DegronID` takes as input (1) a position specific scoring mat
In our experience, degrons that are similar to each other will tend to overlap with each other in which query sequences from the database are scored favorably. Thus, the output from DegronID can be harnessed to cluster degron motifs into related groups. Illustration of the clustering result from Zhang et al. and related visualizations are accessible at https://brandonsie.shinyapps.io/DegronID/.

## Basic Usage
A walkthrough with example data is provided in `example.R`.

## SLURM Usage
This algorithm was written in R and run on a computing cluster that uses Slurm Workload Manager. Necessary R files and example shell scripts are included.

- Creating a query database of amino acid sequences. Save sequences as a .fasta file. `example.fasta`
Expand All @@ -26,4 +29,4 @@ sbatch --export=tile="example_tiles_12.fasta",mat="example_pssm.rds",pos_weight=

## Other Applications

Although we used this method to characterize saturation mutagenesis of degrons, this algorithm could conceivably be applied to various other applications involving saturation mutagenesis, such as for investigation of cross reactivity between TCR epitopes.
Although we used this method to characterize saturation mutagenesis of degrons, this algorithm could conceivably be applied to various other applications involving saturation mutagenesis, such as for investigation of TCR cross reactivity.
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