From a9a928700f17b2bc41b35d370ac047fd3f86fb0a Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 30 Jan 2018 11:41:40 +0000 Subject: [PATCH 01/54] Change to use build stages --- Dockerfile | 28 ++++++++++------------------ 1 file changed, 10 insertions(+), 18 deletions(-) diff --git a/Dockerfile b/Dockerfile index 15e511e..71e4398 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,24 +1,7 @@ -FROM quay.io/wtsicgp/dockstore-cgpmap:2.0.3 - -MAINTAINER keiranmraine@gmail.com - -LABEL uk.ac.sanger.cgp="Cancer Genome Project, Wellcome Trust Sanger Institute" \ - version="2.1.1" \ - description="The CGP WXS pipeline for dockstore.org" +FROM quay.io/wtsicgp/dockstore-cgpmap:3.0.0-rc1 as builder USER root -ENV OPT /opt/wtsi-cgp -ENV PATH $OPT/bin:$PATH -ENV PERL5LIB $OPT/lib/perl5 -ENV LD_LIBRARY_PATH $OPT/lib - -ADD build/apt-build.sh build/ -RUN bash build/apt-build.sh - -ADD build/perllib-build.sh build/ -RUN bash build/perllib-build.sh - ADD build/opt-build.sh build/ RUN bash build/opt-build.sh $OPT @@ -26,6 +9,15 @@ ADD scripts/analysisWXS.sh $OPT/bin/analysisWXS.sh ADD scripts/ds-wrapper.pl $OPT/bin/ds-wrapper.pl RUN chmod a+x $OPT/bin/analysisWXS.sh $OPT/bin/ds-wrapper.pl + +FROM quay.io/wtsicgp/dockstore-cgpmap:3.0.0-rc1 + +MAINTAINER keiranmraine@gmail.com + +LABEL uk.ac.sanger.cgp="Cancer Genome Project, Wellcome Trust Sanger Institute" \ + version="2.1.1" \ + description="The CGP WXS pipeline for dockstore.org" + USER ubuntu WORKDIR /home/ubuntu From 4cc6b8f6e49e3e072c49a4a8f6b271b653de93e6 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 30 Jan 2018 11:41:49 +0000 Subject: [PATCH 02/54] Removed --- build/perllib-build.sh | 5 ----- 1 file changed, 5 deletions(-) delete mode 100644 build/perllib-build.sh diff --git a/build/perllib-build.sh b/build/perllib-build.sh deleted file mode 100644 index 782620a..0000000 --- a/build/perllib-build.sh +++ /dev/null @@ -1,5 +0,0 @@ -#!/bin/bash - -set -eux - -echo "Doing nothing, here for perl libs that won't install through cpanminus" From 8a190070abdd0c6ddfc528c23cbdf249c1dc51b1 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 30 Jan 2018 11:43:46 +0000 Subject: [PATCH 03/54] Add docker ignore --- .dockerignore | 4 ++++ 1 file changed, 4 insertions(+) create mode 100644 .dockerignore diff --git a/.dockerignore b/.dockerignore new file mode 100644 index 0000000..b83d44d --- /dev/null +++ b/.dockerignore @@ -0,0 +1,4 @@ +/examples/ +/git-hooks/ +/*.* +/LICENSE From 4b57756ef9ab26735c21cea94f8102fb2e485ce0 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 30 Jan 2018 11:45:56 +0000 Subject: [PATCH 04/54] Fix #7, rename so all tools are available in composite image --- Dockerfile | 4 +- Dockstore.cwl | 2 +- scripts/ds-cgpwxs.pl | 234 +++++++++++++++++++++++++++++++++++++++++++ 3 files changed, 237 insertions(+), 3 deletions(-) create mode 100644 scripts/ds-cgpwxs.pl diff --git a/Dockerfile b/Dockerfile index 71e4398..9f6873b 100644 --- a/Dockerfile +++ b/Dockerfile @@ -6,8 +6,8 @@ ADD build/opt-build.sh build/ RUN bash build/opt-build.sh $OPT ADD scripts/analysisWXS.sh $OPT/bin/analysisWXS.sh -ADD scripts/ds-wrapper.pl $OPT/bin/ds-wrapper.pl -RUN chmod a+x $OPT/bin/analysisWXS.sh $OPT/bin/ds-wrapper.pl +ADD scripts/ds-cgpwxs.pl $OPT/bin/ds-cgpwxs.pl +RUN chmod a+x $OPT/bin/analysisWXS.sh $OPT/bin/ds-cgpwxs.pl FROM quay.io/wtsicgp/dockstore-cgpmap:3.0.0-rc1 diff --git a/Dockstore.cwl b/Dockstore.cwl index ff1147f..5d1f58b 100644 --- a/Dockstore.cwl +++ b/Dockstore.cwl @@ -120,4 +120,4 @@ outputs: outputBinding: glob: WXS_*_vs_*.timings.tar.gz -baseCommand: ["/opt/wtsi-cgp/bin/ds-wrapper.pl"] +baseCommand: ["/opt/wtsi-cgp/bin/ds-cgpwxs.pl"] diff --git a/scripts/ds-cgpwxs.pl b/scripts/ds-cgpwxs.pl new file mode 100644 index 0000000..20f3940 --- /dev/null +++ b/scripts/ds-cgpwxs.pl @@ -0,0 +1,234 @@ +#!/usr/bin/perl + +use strict; +use Getopt::Long; +use File::Path qw(make_path); +use Pod::Usage qw(pod2usage); +use Data::Dumper; +use autodie qw(:all); +use warnings FATAL => 'all'; + +pod2usage(-verbose => 1, -exitval => 1) if(@ARGV == 0); + +# set defaults +my %opts = ('c'=>0, + 'sc' => q{}, + 'b' => q{} + ,); + +GetOptions( 'h|help' => \$opts{'h'}, + 'm|man' => \$opts{'m'}, + 'r|reference=s' => \$opts{'r'}, + 'a|annot=s' => \$opts{'a'}, + 'si|snv_indel=s' => \$opts{'si'}, + 't|tumour=s' => \$opts{'t'}, + 'n|normal=s' => \$opts{'n'}, + 'e|exclude=s' => \$opts{'e'}, + 'sp|species=s' => \$opts{'sp'}, + 'as|assembly=s' => \$opts{'as'}, + 'sf|snvflag=s' => \$opts{'sf'}, +) or pod2usage(2); + +pod2usage(-verbose => 1, -exitval => 0) if(defined $opts{'h'}); +pod2usage(-verbose => 2, -exitval => 0) if(defined $opts{'m'}); + +delete $opts{'h'}; +delete $opts{'m'}; +delete $opts{'sp'} if(! defined $opts{'sp'} || length $opts{'sp'} == 0); +delete $opts{'as'} if(! defined $opts{'as'} || length $opts{'as'} == 0); + +## read species/assembly from bam headers +my ($mt_species, $mt_assembly) = species_assembly_from_xam($opts{'t'}); +my ($wt_species, $wt_assembly) = species_assembly_from_xam($opts{'n'}); +if($mt_species ne $wt_species) { + warn "WARN: Species mismatch between T/N [CR|B]AM headers\n"; + if(!defined $opts{'sp'}) { + die "ERROR: Please define species to handle this mismatch\n"; + } +} +elsif($mt_species ne q{}) { + $opts{'sp'} = $mt_species; +} +if(!defined $opts{'sp'}) { + die "ERROR: Please define species, not found in [CR|B]AM headers.\n"; +} + +if($mt_assembly ne $wt_assembly) { + warn "WARN: Assembly mismatch between T/N [CR|B]AM headers\n"; + if(!defined $opts{'as'}) { + die "ERROR: Please define assembly to handle this mismatch\n"; + } +} +elsif($mt_assembly ne q{}) { + $opts{'as'} = $mt_assembly; +} +if(!defined $opts{'as'}) { + die "ERROR: Please define assembly, not found in [CR|B]AM headers.\n"; +} + +printf "Options loaded: \n%s\n",Dumper(\%opts); + +## unpack the reference area: +my $ref_area = $ENV{HOME}.'/reference_files'; + +ref_unpack($ref_area, $opts{'r'}); +ref_unpack($ref_area, $opts{'a'}); +ref_unpack($ref_area, $opts{'si'}); + +## now complete the caveman flaging file correctly +my $ini = add_species_flag_ini($opts{'sp'}, "$ref_area/caveman/flag.vcf.config.WXS.ini"); + +my $run_file = $ENV{HOME}.'/run.params'; +open my $FH,'>',$run_file or die "Failed to write to $run_file: $!"; +# Force explicit checking of file flush +print $FH "export PCAP_THREADED_NO_SCRIPT=1\n"; +print $FH "export PCAP_THREADED_FORCE_SYNC=1\n"; +print $FH "export PCAP_THREADED_LOADBACKOFF=1\n"; +# hard-coded +printf $FH "PROTOCOL=WXS\n"; +# required options +printf $FH "OUTPUT_DIR='%s'\n", $ENV{HOME}; +printf $FH "REF_BASE='%s'\n", $ref_area; +printf $FH "BAM_MT='%s'\n", $opts{'t'}; +printf $FH "BAM_WT='%s'\n", $opts{'n'}; +printf $FH "PINDEL_EXCLUDE='%s'\n", $opts{'e'}; +printf $FH "SPECIES='%s'\n", $opts{'sp'}; +printf $FH "ASSEMBLY='%s'\n", $opts{'as'}; +printf $FH "SNVFLAG='%s'\n", $ini; +close $FH; + +exec('analysisWXS.sh'); # I will never return to the perl code + +sub add_species_flag_ini { + my ($species, $ini_in) = @_; + $species =~ s/ /_/g; + $species = uc $species; + my $ini_out = $ENV{HOME}.'/flag.vcf.config.WXS.ini'; + open my $IN, '<', $ini_in; + open my $OUT,'>',$ini_out; + while(my $line = <$IN>) { + $line =~ s/^\[HUMAN_/[${species}_/; + print $OUT $line; + } + close $OUT; + close $IN; + return $ini_out; +} + +sub species_assembly_from_xam { + my $xam = shift; + my %assembly_set; + my %species_set; + open my $SAM, '-|', "samtools view -H $xam" or die $!; + while(my $line = <$SAM>) { + next unless($line =~ m/^\@SQ/); + chomp $line; + $line .= "\t"; # simplify matching + if($line =~ m/\tAS:([^\t]+)\t/) { + $assembly_set{$1}++; + } + if($line =~ m/\SP:([^\t]+)\t/) { + $species_set{$1}++; + } + } + close $SAM; + my $species = q{}; + my $max_val = 0; + for(keys %species_set) { + if($species_set{$_} > $max_val) { + $max_val = $species_set{$_}; + $species = $_; + } + } + my $assembly = q{}; + $max_val = 0; + for(keys %assembly_set) { + if($assembly_set{$_} > $max_val) { + $max_val = $assembly_set{$_}; + $assembly = $_; + } + } + return ($species, $assembly); +} + +sub ref_unpack { + my ($ref_area, $item) = @_; + make_path($ref_area) unless(-d $ref_area); + my $untar = sprintf 'tar --strip-components 1 -C %s -zxvf %s', $ref_area, $item; + system($untar) && die $!; + return 1; +} + +__END__ + + +=head1 NAME + +dh-wrapper.pl - Generate the param file and execute analysisWXS.sh (for dockstore) + +=head1 SYNOPSIS + +dh-wrapper.pl [options] [file(s)...] + + Required parameters: + -reference -r Path to core reference tar.gz + -annot -a Path to VAGrENT*.tar.gz + -snv_indel -si Path to SNV_INDEL*.tar.gz + -tumour -t Tumour [CR|B]AM file + -normal -n Normal [CR|B]AM file + -exclude -e Exclude these contigs from pindel analysis + e.g. NC_007605,hs37d5,GL% + + Optional parameters + -species -sp Species name (may require quoting) + -assembly -a Reference assembly + + Other: + -help -h Brief help message. + -man -m Full documentation. + +=head1 DESCRIPTION + +Wrapper script to map dockstore cwl inputs to PARAMS file used by underlying code. + +=head1 OPTION DETAILS + +=over 4 + +=item B<-reference> + +Path to mapping tar.gz reference files + +=item B<-annot> + +Path to VAGrENT*.tar.gz + +=item B<-snv_indel> + +Path to Path to SNV_INDEL*.tar.gz + +=item B<-tumour> + +Path to tumour BAM or CRAM file with co-located index and BAS file. + +=item B<-normal> + +Path to normal BAM or CRAM file with co-located index and BAS file. + +=item B<-exclude> + +Contigs to be excluded from Pindel analysis, csv, use '%' for wildcard. + +=item B<-species> + +Specify overriding species, by default will select the most prevelant entry in +[CR|B]AM header (to cope with inclusion of viral/decoy sequences). + +=item B<-assembly> + +Specify overriding assembly, by default will select the most prevelant entry in +[CR|B]AM header (to cope with inclusion of viral/decoy sequences). + +=back + +=cut From d9a91c22b24e1b38192059c3adb669ce142c900c Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 30 Jan 2018 11:51:20 +0000 Subject: [PATCH 05/54] Minor change to build versions --- build/opt-build.sh | 7 +- scripts/ds-wrapper.pl | 234 ------------------------------------------ 2 files changed, 3 insertions(+), 238 deletions(-) delete mode 100644 scripts/ds-wrapper.pl diff --git a/build/opt-build.sh b/build/opt-build.sh index 5af72d1..ed1bb47 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -13,13 +13,12 @@ VER_CGPVCF="v2.2.0" VER_VCFTOOLS="0.1.14" # cgpPindel -VER_CGPPINDEL="v2.2.4" +VER_CGPPINDEL="feature/readsVsFragments" # cgpCaVEManPostProcessing VER_CGPCAVEPOSTPROC="1.6.9" -# Warning bedtools 2.24.0 and 2.25.0 have a swapped usage in coverageBed -# No upgrades until [this ticket](https://github.com/arq5x/bedtools2/issues/319) is resolved -VER_BEDTOOLS="2.21.0" +# if issues found downgrade to 2.23.0 but can't find any use of bedtools coverage +VER_BEDTOOLS="2.27.1" # cgpCaVEManWrapper VER_CGPCAVEWRAP="1.12.0" diff --git a/scripts/ds-wrapper.pl b/scripts/ds-wrapper.pl deleted file mode 100644 index 20f3940..0000000 --- a/scripts/ds-wrapper.pl +++ /dev/null @@ -1,234 +0,0 @@ -#!/usr/bin/perl - -use strict; -use Getopt::Long; -use File::Path qw(make_path); -use Pod::Usage qw(pod2usage); -use Data::Dumper; -use autodie qw(:all); -use warnings FATAL => 'all'; - -pod2usage(-verbose => 1, -exitval => 1) if(@ARGV == 0); - -# set defaults -my %opts = ('c'=>0, - 'sc' => q{}, - 'b' => q{} - ,); - -GetOptions( 'h|help' => \$opts{'h'}, - 'm|man' => \$opts{'m'}, - 'r|reference=s' => \$opts{'r'}, - 'a|annot=s' => \$opts{'a'}, - 'si|snv_indel=s' => \$opts{'si'}, - 't|tumour=s' => \$opts{'t'}, - 'n|normal=s' => \$opts{'n'}, - 'e|exclude=s' => \$opts{'e'}, - 'sp|species=s' => \$opts{'sp'}, - 'as|assembly=s' => \$opts{'as'}, - 'sf|snvflag=s' => \$opts{'sf'}, -) or pod2usage(2); - -pod2usage(-verbose => 1, -exitval => 0) if(defined $opts{'h'}); -pod2usage(-verbose => 2, -exitval => 0) if(defined $opts{'m'}); - -delete $opts{'h'}; -delete $opts{'m'}; -delete $opts{'sp'} if(! defined $opts{'sp'} || length $opts{'sp'} == 0); -delete $opts{'as'} if(! defined $opts{'as'} || length $opts{'as'} == 0); - -## read species/assembly from bam headers -my ($mt_species, $mt_assembly) = species_assembly_from_xam($opts{'t'}); -my ($wt_species, $wt_assembly) = species_assembly_from_xam($opts{'n'}); -if($mt_species ne $wt_species) { - warn "WARN: Species mismatch between T/N [CR|B]AM headers\n"; - if(!defined $opts{'sp'}) { - die "ERROR: Please define species to handle this mismatch\n"; - } -} -elsif($mt_species ne q{}) { - $opts{'sp'} = $mt_species; -} -if(!defined $opts{'sp'}) { - die "ERROR: Please define species, not found in [CR|B]AM headers.\n"; -} - -if($mt_assembly ne $wt_assembly) { - warn "WARN: Assembly mismatch between T/N [CR|B]AM headers\n"; - if(!defined $opts{'as'}) { - die "ERROR: Please define assembly to handle this mismatch\n"; - } -} -elsif($mt_assembly ne q{}) { - $opts{'as'} = $mt_assembly; -} -if(!defined $opts{'as'}) { - die "ERROR: Please define assembly, not found in [CR|B]AM headers.\n"; -} - -printf "Options loaded: \n%s\n",Dumper(\%opts); - -## unpack the reference area: -my $ref_area = $ENV{HOME}.'/reference_files'; - -ref_unpack($ref_area, $opts{'r'}); -ref_unpack($ref_area, $opts{'a'}); -ref_unpack($ref_area, $opts{'si'}); - -## now complete the caveman flaging file correctly -my $ini = add_species_flag_ini($opts{'sp'}, "$ref_area/caveman/flag.vcf.config.WXS.ini"); - -my $run_file = $ENV{HOME}.'/run.params'; -open my $FH,'>',$run_file or die "Failed to write to $run_file: $!"; -# Force explicit checking of file flush -print $FH "export PCAP_THREADED_NO_SCRIPT=1\n"; -print $FH "export PCAP_THREADED_FORCE_SYNC=1\n"; -print $FH "export PCAP_THREADED_LOADBACKOFF=1\n"; -# hard-coded -printf $FH "PROTOCOL=WXS\n"; -# required options -printf $FH "OUTPUT_DIR='%s'\n", $ENV{HOME}; -printf $FH "REF_BASE='%s'\n", $ref_area; -printf $FH "BAM_MT='%s'\n", $opts{'t'}; -printf $FH "BAM_WT='%s'\n", $opts{'n'}; -printf $FH "PINDEL_EXCLUDE='%s'\n", $opts{'e'}; -printf $FH "SPECIES='%s'\n", $opts{'sp'}; -printf $FH "ASSEMBLY='%s'\n", $opts{'as'}; -printf $FH "SNVFLAG='%s'\n", $ini; -close $FH; - -exec('analysisWXS.sh'); # I will never return to the perl code - -sub add_species_flag_ini { - my ($species, $ini_in) = @_; - $species =~ s/ /_/g; - $species = uc $species; - my $ini_out = $ENV{HOME}.'/flag.vcf.config.WXS.ini'; - open my $IN, '<', $ini_in; - open my $OUT,'>',$ini_out; - while(my $line = <$IN>) { - $line =~ s/^\[HUMAN_/[${species}_/; - print $OUT $line; - } - close $OUT; - close $IN; - return $ini_out; -} - -sub species_assembly_from_xam { - my $xam = shift; - my %assembly_set; - my %species_set; - open my $SAM, '-|', "samtools view -H $xam" or die $!; - while(my $line = <$SAM>) { - next unless($line =~ m/^\@SQ/); - chomp $line; - $line .= "\t"; # simplify matching - if($line =~ m/\tAS:([^\t]+)\t/) { - $assembly_set{$1}++; - } - if($line =~ m/\SP:([^\t]+)\t/) { - $species_set{$1}++; - } - } - close $SAM; - my $species = q{}; - my $max_val = 0; - for(keys %species_set) { - if($species_set{$_} > $max_val) { - $max_val = $species_set{$_}; - $species = $_; - } - } - my $assembly = q{}; - $max_val = 0; - for(keys %assembly_set) { - if($assembly_set{$_} > $max_val) { - $max_val = $assembly_set{$_}; - $assembly = $_; - } - } - return ($species, $assembly); -} - -sub ref_unpack { - my ($ref_area, $item) = @_; - make_path($ref_area) unless(-d $ref_area); - my $untar = sprintf 'tar --strip-components 1 -C %s -zxvf %s', $ref_area, $item; - system($untar) && die $!; - return 1; -} - -__END__ - - -=head1 NAME - -dh-wrapper.pl - Generate the param file and execute analysisWXS.sh (for dockstore) - -=head1 SYNOPSIS - -dh-wrapper.pl [options] [file(s)...] - - Required parameters: - -reference -r Path to core reference tar.gz - -annot -a Path to VAGrENT*.tar.gz - -snv_indel -si Path to SNV_INDEL*.tar.gz - -tumour -t Tumour [CR|B]AM file - -normal -n Normal [CR|B]AM file - -exclude -e Exclude these contigs from pindel analysis - e.g. NC_007605,hs37d5,GL% - - Optional parameters - -species -sp Species name (may require quoting) - -assembly -a Reference assembly - - Other: - -help -h Brief help message. - -man -m Full documentation. - -=head1 DESCRIPTION - -Wrapper script to map dockstore cwl inputs to PARAMS file used by underlying code. - -=head1 OPTION DETAILS - -=over 4 - -=item B<-reference> - -Path to mapping tar.gz reference files - -=item B<-annot> - -Path to VAGrENT*.tar.gz - -=item B<-snv_indel> - -Path to Path to SNV_INDEL*.tar.gz - -=item B<-tumour> - -Path to tumour BAM or CRAM file with co-located index and BAS file. - -=item B<-normal> - -Path to normal BAM or CRAM file with co-located index and BAS file. - -=item B<-exclude> - -Contigs to be excluded from Pindel analysis, csv, use '%' for wildcard. - -=item B<-species> - -Specify overriding species, by default will select the most prevelant entry in -[CR|B]AM header (to cope with inclusion of viral/decoy sequences). - -=item B<-assembly> - -Specify overriding assembly, by default will select the most prevelant entry in -[CR|B]AM header (to cope with inclusion of viral/decoy sequences). - -=back - -=cut From fd0a80160a8790fa5b943750a1a779f74a0f2c0b Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 30 Jan 2018 13:41:42 +0000 Subject: [PATCH 06/54] Clean up format of chagnes --- CHANGES.md | 50 ++++++++++++++++++++++++++++++++++++-------------- 1 file changed, 36 insertions(+), 14 deletions(-) diff --git a/CHANGES.md b/CHANGES.md index 7ab362f..3e4e70c 100644 --- a/CHANGES.md +++ b/CHANGES.md @@ -1,47 +1,69 @@ -### 2.1.0 +# CHANGES + +## NEXT + +* See dockstore-cgpmap v3.0.0 (primarily adds mismatchQc) +* CaVEMan and cgpPindel updated to use freagment based counting. +* Using build stages to shrink images. + +## 2.1.0 + * Fixes various sort order issues in CaVEMan, cgpPindel and VAGrENT affecting comparison of results from same inputs. -### 2.0.7 +## 2.0.7 + * Bumps cgpPindel to [v2.2.3](https://github.com/cancerit/cgpPindel/releases/tag/v2.2.3) to fix bug in DI event collation. -### 2.0.6 +## 2.0.6 + * Update base image to dockstore-cgpmap:2.0.3 (new PCAP-core for load management) * Bump cgpCaVEManWrapper to gain access to split size tuning (not used here) -### 2.0.5 +## 2.0.5 + Very minor modifications to reduce potential issues from CPU oversubscription. -### 2.0.4 +## 2.0.4 + Large reduction in temp space and I/O for cgpPindel. See [cgpPindel-v2.2.0](https://github.com/cancerit/cgpPindel/releases/tag/v2.2.0) -### 2.0.3 +## 2.0.3 + * Fixed cgpPindel -### 2.0.2 +## 2.0.2 + * Update cgpPindel to reduce usage of Capture::Tiny apparent cause of some failures. -### 2.0.1 +## 2.0.1 + * Test data in `examples/analysis_config.local.json` moved to a non-expiring location. -### 2.0.0 +## 2.0.0 + * See [dockstore-cgpmap:2.0.0](https://github.com/cancerit/dockstore-cgpmap/releases/tag/2.0.0) * Streamlined install process to reduce build time and size of image. * Fix to SNV flagging - was not being applied previously. -### 1.0.4 +## 1.0.4 + * Updates base image to add load management facilities via ENV variables. -### 1.0.3 +## 1.0.3 + * Add params file to output bindings -### 1.0.2 +## 1.0.2 + * Fix up output files slightly * Update base image for dependency (PCAP-core etc) -### 1.0.1 +## 1.0.1 + * Fixes handling of species/assembly * Update base image (PCAP-core etc) -### 1.0.0 +## 1.0.0 + Initial release of flow From b7bb19ec884777d92cc2fd5f606be7f67fc5ba37 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 30 Jan 2018 14:03:05 +0000 Subject: [PATCH 07/54] Format cleanup --- README.md | 35 ++++++++++++++++++----------------- 1 file changed, 18 insertions(+), 17 deletions(-) diff --git a/README.md b/README.md index 98ac882..3ad0a5b 100644 --- a/README.md +++ b/README.md @@ -1,5 +1,5 @@ -dockstore-cgpwxs -====== +# dockstore-cgpwxs + `dockstore-cgpwxs` provides a complete multi threaded WXS analysis for SNV and INDEL variants with associated annotation of VCF files. This has been packaged specifically for use with the [Dockstore.org](https://dockstore.org/) framework. [![Docker Repository on Quay](https://quay.io/repository/wtsicgp/dockstore-cgpwxs/status "Docker Repository on Quay")](https://quay.io/repository/wtsicgp/dockstore-cgpwxs) @@ -10,10 +10,10 @@ dockstore-cgpwxs Required input files are 1. Tumour BAM file -2. Normal BAM file -3. Core reference archive (e.g. [core_ref_GRCh37d5.tar.gz](ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/)) -4. WXS reference archive (e.g. [SNV_INDEL_ref_GRCh37d5.tar.gz](ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/)) -5. VAGrENT (annotation) reference archive (e.g. [VAGrENT_ref_GRCh37d5_ensembl_75.tar.gz](ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/)) +1. Normal BAM file +1. Core reference archive (e.g. [core_ref_GRCh37d5.tar.gz](ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/)) +1. WXS reference archive (e.g. [SNV_INDEL_ref_GRCh37d5.tar.gz](ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/)) +1. VAGrENT (annotation) reference archive (e.g. [VAGrENT_ref_GRCh37d5_ensembl_75.tar.gz](ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/)) Inputs 1&2 are expected to have been mapped using [dockstore-cgpmap](https://dockstore.org/containers/quay.io/wtsicgp/dockstore-cgpmap). @@ -22,22 +22,22 @@ All the SNV variants do not pass filtering (several only fail as not in a gene f Please check the Wiki then raise an issue if you require additional information on how to generate your own reference files. Much of this information is available on the individual algorithm pages. -Release process -=============== +## Release process + This project is maintained using HubFlow. 1. Make appropriate changes -2. Bump version in `Dockerfile` and `Dockstore.cwl` -3. Push changes -4. Check state on Travis -5. Generate the release (add notes to GitHub) -6. Confirm that image has been built on [quay.io](https://quay.io/repository/wtsicgp/dockstore-cgpwxs?tab=builds) -7. Update the [dockstore](https://dockstore.org/containers/quay.io/wtsicgp/dockstore-cgpwxs) entry, see [their docs](https://dockstore.org/docs/getting-started-with-dockstore). +1. Bump version in `Dockerfile` and `Dockstore.cwl` +1. Push changes +1. Check state on Travis +1. Generate the release (add notes to GitHub) +1. Confirm that image has been built on [quay.io](https://quay.io/repository/wtsicgp/dockstore-cgpwxs?tab=builds) +1. Update the [dockstore](https://dockstore.org/containers/quay.io/wtsicgp/dockstore-cgpwxs) entry, see [their docs](https://dockstore.org/docs/getting-started-with-dockstore). -LICENCE -======= +## LICENCE -Copyright (c) 2016-2017 Genome Research Ltd. +``` +Copyright (c) 2016-2018 Genome Research Ltd. Author: Cancer Genome Project @@ -65,3 +65,4 @@ reads ‘Copyright (c) 2005, 2007, 2008, 2009, 2011, 2012’ and a copyright statement that reads ‘Copyright (c) 2005-2012’ should be interpreted as being identical to a statement that reads ‘Copyright (c) 2005, 2006, 2007, 2008, 2009, 2010, 2011, 2012’." +``` From 5c2ef82a2605ee1e1c906691752365afe34e93ac Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 30 Jan 2018 14:03:44 +0000 Subject: [PATCH 08/54] cgpVcf, vcftools update and related changes --- build/opt-build.sh | 9 ++++----- 1 file changed, 4 insertions(+), 5 deletions(-) diff --git a/build/opt-build.sh b/build/opt-build.sh index ed1bb47..1a8040d 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -9,8 +9,8 @@ fi set -u # cgpVcf -VER_CGPVCF="v2.2.0" -VER_VCFTOOLS="0.1.14" +VER_CGPVCF="v2.2.1" +VER_VCFTOOLS="0.1.15" # cgpPindel VER_CGPPINDEL="feature/readsVsFragments" @@ -74,10 +74,9 @@ set -u if [ ! -e $SETUP_DIR/vcftools.success ]; then curl -sSL --retry 10 https://github.com/vcftools/vcftools/releases/download/v${VER_VCFTOOLS}/vcftools-${VER_VCFTOOLS}.tar.gz > distro.tar.gz rm -rf distro/* - tar --strip-components 1 -C distro -xzf distro.tar.gz + tar --strip-components 2 -C distro -xzf distro.tar.gz cd distro - curl -sSL https://raw.githubusercontent.com/cancerit/cgpVcf/${VER_CGPVCF}/patches/vcfToolsProcessLog.diff | patch src/perl/Vcf.pm - ./configure --prefix=$INST_PATH --with-pmdir=$INST_PATH/lib/perl5 + ./configure --prefix=$INST_PATH --with-pmdir=lib/perl5 make -j$CPU make install cd $SETUP_DIR From 9dc514228d44c5092f745fa009e14f078a927fa5 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 30 Jan 2018 14:04:22 +0000 Subject: [PATCH 09/54] Build stages for smaller images, new base image for builder --- Dockerfile | 46 +++++++++++++++++++++++++++++++++++++++++++--- Dockstore.cwl | 2 +- 2 files changed, 44 insertions(+), 4 deletions(-) diff --git a/Dockerfile b/Dockerfile index 9f6873b..fc28039 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,7 +1,23 @@ -FROM quay.io/wtsicgp/dockstore-cgpmap:3.0.0-rc1 as builder +FROM quay.io/wtsicgp/dockstore-cgpmap:3.0.0-rc2 as builder USER root +ENV OPT /opt/wtsi-cgp +ENV PATH $OPT/bin:$PATH +ENV PERL5LIB $OPT/lib/perl5 +ENV LD_LIBRARY_PATH $OPT/lib + +RUN apt-get -yq update +RUN apt-get install -yq --no-install-recommends\ + g++\ + make\ + gcc\ + pkg-config\ + zlib1g-dev\ + python + +# python only for building bedtools, not needed to use + ADD build/opt-build.sh build/ RUN bash build/opt-build.sh $OPT @@ -10,14 +26,38 @@ ADD scripts/ds-cgpwxs.pl $OPT/bin/ds-cgpwxs.pl RUN chmod a+x $OPT/bin/analysisWXS.sh $OPT/bin/ds-cgpwxs.pl -FROM quay.io/wtsicgp/dockstore-cgpmap:3.0.0-rc1 +FROM ubuntu:16.04 MAINTAINER keiranmraine@gmail.com LABEL uk.ac.sanger.cgp="Cancer Genome Project, Wellcome Trust Sanger Institute" \ - version="2.1.1" \ + version="3.0.0-rc1" \ description="The CGP WXS pipeline for dockstore.org" +ENV OPT /opt/wtsi-cgp +ENV PATH $OPT/bin:$PATH +ENV PERL5LIB $OPT/lib/perl5 +ENV LD_LIBRARY_PATH $OPT/lib + +RUN apt-get -yq update +RUN apt-get install -yq --no-install-recommends\ + apt-transport-https\ + curl\ + ca-certificates\ + libperlio-gzip-perl\ + bzip2\ + psmisc\ + time\ + zlib1g\ + liblzma5\ + libncurses5 + +RUN mkdir -p $OPT +COPY --from=builder $OPT $OPT + +## USER CONFIGURATION +RUN adduser --disabled-password --gecos '' ubuntu && chsh -s /bin/bash && mkdir -p /home/ubuntu + USER ubuntu WORKDIR /home/ubuntu diff --git a/Dockstore.cwl b/Dockstore.cwl index 5d1f58b..95db59c 100644 --- a/Dockstore.cwl +++ b/Dockstore.cwl @@ -19,7 +19,7 @@ dct:creator: requirements: - class: DockerRequirement - dockerPull: "quay.io/wtsicgp/dockstore-cgpwxs:2.1.1" + dockerPull: "quay.io/wtsicgp/dockstore-cgpwxs:3.0.0-rc1" hints: - class: ResourceRequirement From 53fa370c8440a3d66b3a4105d88d88aa97584363 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 31 Jan 2018 12:59:57 +0000 Subject: [PATCH 10/54] Update to rc's and changed ref file --- build/opt-build.sh | 8 ++++---- examples/analysis_config.local.json | 2 +- 2 files changed, 5 insertions(+), 5 deletions(-) diff --git a/build/opt-build.sh b/build/opt-build.sh index 1a8040d..930e25b 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -13,16 +13,16 @@ VER_CGPVCF="v2.2.1" VER_VCFTOOLS="0.1.15" # cgpPindel -VER_CGPPINDEL="feature/readsVsFragments" +VER_CGPPINDEL="v3.0.0-rc1" # cgpCaVEManPostProcessing -VER_CGPCAVEPOSTPROC="1.6.9" +VER_CGPCAVEPOSTPROC="1.8.0-rc1" # if issues found downgrade to 2.23.0 but can't find any use of bedtools coverage VER_BEDTOOLS="2.27.1" # cgpCaVEManWrapper -VER_CGPCAVEWRAP="1.12.0" -VER_CAVEMAN="1.12.0" +VER_CGPCAVEWRAP="1.13.0-rc1" +VER_CAVEMAN="1.13.0-rc1" # VAGrENT VER_VAGRENT="v3.2.3" diff --git a/examples/analysis_config.local.json b/examples/analysis_config.local.json index 2795942..21a33f2 100644 --- a/examples/analysis_config.local.json +++ b/examples/analysis_config.local.json @@ -8,7 +8,7 @@ "class": "File" }, "snv_indel": { - "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/SNV_INDEL_ref_GRCh37d5.tar.gz", + "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/SNV_INDEL_ref_GRCh37d5-fragment.tar.gz", "class": "File" }, "tumour": { From e3f61c01ff33ec8dcb0901ed8824d6e3da2dacaa Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 31 Jan 2018 13:00:16 +0000 Subject: [PATCH 11/54] Resolves 9 --- scripts/ds-cgpwxs.pl | 14 ++++++++++++++ 1 file changed, 14 insertions(+) diff --git a/scripts/ds-cgpwxs.pl b/scripts/ds-cgpwxs.pl index 20f3940..0390dfb 100644 --- a/scripts/ds-cgpwxs.pl +++ b/scripts/ds-cgpwxs.pl @@ -68,6 +68,20 @@ printf "Options loaded: \n%s\n",Dumper(\%opts); +# check all inputs exist and have relevant files (bai/csi/crai + bas) +for my $base_af(($opts{'t'}, $opts{'n'})) { + if($base_af =~ m/\.bam$/) { + # must be bai or csi index + die "ERROR: No index (bai/csi) found for $base_af\n" unless(-e "$base_af.bai" || -e "$base_af.csi"); + } + else { + # cram only have crai as index + die "ERROR: No index (crai) found for $base_af\n" unless(-e "$base_af.crai"); + } + warn "WARN: No 'bas' file found for $base_af, this is generated by the mapping flow\n". + "\tWill generate internally but suboptimal\n" unless(-e "$base_af.bas"); +} + ## unpack the reference area: my $ref_area = $ENV{HOME}.'/reference_files'; From afaaea74fe411cc5695fabee6abd70ef52afc68c Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 31 Jan 2018 14:42:13 +0000 Subject: [PATCH 12/54] Resolves #8, plus some doc corrections --- README.md | 16 ++++++++++++++-- scripts/ds-cgpwxs.pl | 2 ++ 2 files changed, 16 insertions(+), 2 deletions(-) diff --git a/README.md b/README.md index 3ad0a5b..84651a4 100644 --- a/README.md +++ b/README.md @@ -9,8 +9,11 @@ Required input files are -1. Tumour BAM file -1. Normal BAM file +Note: cram and csi/crai will not work via Dockstore/CWL yet but direct exec of `scripts/ds-cgpwxs.pl` +will allow use of these. + +1. Tumour BAM/CRAM file (plus bai/csi/crai + bas) +1. Normal BAM/CRAM file (plus bai/csi/crai + bas) 1. Core reference archive (e.g. [core_ref_GRCh37d5.tar.gz](ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/)) 1. WXS reference archive (e.g. [SNV_INDEL_ref_GRCh37d5.tar.gz](ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/)) 1. VAGrENT (annotation) reference archive (e.g. [VAGrENT_ref_GRCh37d5_ensembl_75.tar.gz](ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/)) @@ -22,6 +25,15 @@ All the SNV variants do not pass filtering (several only fail as not in a gene f Please check the Wiki then raise an issue if you require additional information on how to generate your own reference files. Much of this information is available on the individual algorithm pages. +## Usable Cores + +When running outside of a docker container you can set the number of CPUs via: + +* `export CPU=N` +* `-threads|-t` option of `ds-cgpmap.pl` + +If not set detects available cores on system. + ## Release process This project is maintained using HubFlow. diff --git a/scripts/ds-cgpwxs.pl b/scripts/ds-cgpwxs.pl index 0390dfb..02ba2a7 100644 --- a/scripts/ds-cgpwxs.pl +++ b/scripts/ds-cgpwxs.pl @@ -27,6 +27,7 @@ 'sp|species=s' => \$opts{'sp'}, 'as|assembly=s' => \$opts{'as'}, 'sf|snvflag=s' => \$opts{'sf'}, + 't|threads:i' => \$opts{'t'}, ) or pod2usage(2); pod2usage(-verbose => 1, -exitval => 0) if(defined $opts{'h'}); @@ -109,6 +110,7 @@ printf $FH "SPECIES='%s'\n", $opts{'sp'}; printf $FH "ASSEMBLY='%s'\n", $opts{'as'}; printf $FH "SNVFLAG='%s'\n", $ini; +printf $FH "CPU=%d\n", $opts{'t'} if(defined $opts{'t'}); close $FH; exec('analysisWXS.sh'); # I will never return to the perl code From db87320e831f47bbb4672731ef180234b3656127 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 31 Jan 2018 20:20:02 +0000 Subject: [PATCH 13/54] Correct to prevent opt shortname clash --- scripts/ds-cgpwxs.pl | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/scripts/ds-cgpwxs.pl b/scripts/ds-cgpwxs.pl index 02ba2a7..c1cf16e 100644 --- a/scripts/ds-cgpwxs.pl +++ b/scripts/ds-cgpwxs.pl @@ -27,7 +27,7 @@ 'sp|species=s' => \$opts{'sp'}, 'as|assembly=s' => \$opts{'as'}, 'sf|snvflag=s' => \$opts{'sf'}, - 't|threads:i' => \$opts{'t'}, + 'c|cores:i' => \$opts{'c'}, ) or pod2usage(2); pod2usage(-verbose => 1, -exitval => 0) if(defined $opts{'h'}); @@ -110,7 +110,7 @@ printf $FH "SPECIES='%s'\n", $opts{'sp'}; printf $FH "ASSEMBLY='%s'\n", $opts{'as'}; printf $FH "SNVFLAG='%s'\n", $ini; -printf $FH "CPU=%d\n", $opts{'t'} if(defined $opts{'t'}); +printf $FH "CPU=%d\n", $opts{'c'} if(defined $opts{'c'}); close $FH; exec('analysisWXS.sh'); # I will never return to the perl code From 86dac92fbcb8c1bbad9c8151a9ca24beb0690399 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 31 Jan 2018 20:20:43 +0000 Subject: [PATCH 14/54] Correct doc due to db87320 --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index 84651a4..249caab 100644 --- a/README.md +++ b/README.md @@ -30,7 +30,7 @@ Please check the Wiki then raise an issue if you require additional information When running outside of a docker container you can set the number of CPUs via: * `export CPU=N` -* `-threads|-t` option of `ds-cgpmap.pl` +* `-cores|-c` option of `ds-cgpmap.pl` If not set detects available cores on system. From 18e7e16206a103217b6f324c4c3e9c3c3e9f3875 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 31 Jan 2018 20:45:06 +0000 Subject: [PATCH 15/54] Changes to make cram and various index types acceptable --- Dockstore.cwl | 30 +++++++++---- ...nalysis_config.local.json => bam_bai.json} | 8 ++++ examples/bam_csi.json | 43 +++++++++++++++++++ examples/cram_crai.json | 43 +++++++++++++++++++ scripts/analysisWXS.sh | 29 ++++++++++--- scripts/ds-cgpwxs.pl | 4 ++ 6 files changed, 144 insertions(+), 13 deletions(-) rename examples/{analysis_config.local.json => bam_bai.json} (77%) create mode 100644 examples/bam_csi.json create mode 100644 examples/cram_crai.json diff --git a/Dockstore.cwl b/Dockstore.cwl index 95db59c..88abfd3 100644 --- a/Dockstore.cwl +++ b/Dockstore.cwl @@ -55,23 +55,37 @@ inputs: tumour: type: File secondaryFiles: - - .bai - .bas - doc: "Tumour BAM or CRAM file" + doc: "Tumour alignments file [bam|cram]" inputBinding: prefix: -tumour position: 4 separate: true + tumourIdx: + type: File + doc: "Tumour alignment file index [bai|csi|crai]" + inputBinding: + prefix: -tidx + position: 5 + separate: true + normal: type: File secondaryFiles: - - .bai - .bas - doc: "Normal BAM or CRAM file" + doc: "Normal alignments file [bam|cram]" inputBinding: prefix: -normal - position: 5 + position: 6 + separate: true + + normalIdx: + type: File + doc: "Normal alignment file index" + inputBinding: + prefix: -normal + position: 7 separate: true exclude: @@ -79,7 +93,7 @@ inputs: doc: "Contigs to block during indel analysis" inputBinding: prefix: -exclude - position: 6 + position: 8 separate: true shellQuote: true @@ -89,7 +103,7 @@ inputs: default: '' inputBinding: prefix: -species - position: 7 + position: 9 separate: true shellQuote: true @@ -99,7 +113,7 @@ inputs: default: '' inputBinding: prefix: -assembly - position: 8 + position: 10 separate: true shellQuote: true diff --git a/examples/analysis_config.local.json b/examples/bam_bai.json similarity index 77% rename from examples/analysis_config.local.json rename to examples/bam_bai.json index 21a33f2..5206b71 100644 --- a/examples/analysis_config.local.json +++ b/examples/bam_bai.json @@ -15,10 +15,18 @@ "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwxs/COLO-829_ex.bam", "class": "File" }, + "tumourIdx": { + "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwxs/COLO-829_ex.bam.bai", + "class": "File" + }, "normal": { "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwxs/COLO-829-BL_ex.bam", "class": "File" }, + "normalIdx": { + "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwxs/COLO-829-BL_ex.bam.bai", + "class": "File" + }, "exclude": "NC_007605,hs37d5,GL%", "result_archive": { "path": "/tmp/result_WXS.tar.gz", diff --git a/examples/bam_csi.json b/examples/bam_csi.json new file mode 100644 index 0000000..b5e7d93 --- /dev/null +++ b/examples/bam_csi.json @@ -0,0 +1,43 @@ +{ + "reference": { + "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/core_ref_GRCh37d5.tar.gz", + "class": "File" + }, + "annot": { + "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/VAGrENT_ref_GRCh37d5_ensembl_75.tar.gz", + "class": "File" + }, + "snv_indel": { + "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/SNV_INDEL_ref_GRCh37d5-fragment.tar.gz", + "class": "File" + }, + "tumour": { + "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwxs/COLO-829_ex.bam", + "class": "File" + }, + "tumourIdx": { + "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwxs/COLO-829_ex.bam.csi", + "class": "File" + }, + "normal": { + "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwxs/COLO-829-BL_ex.bam", + "class": "File" + }, + "normalIdx": { + "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwxs/COLO-829-BL_ex.bam.csi", + "class": "File" + }, + "exclude": "NC_007605,hs37d5,GL%", + "result_archive": { + "path": "/tmp/result_WXS.tar.gz", + "class": "File" + }, + "timings": { + "path": "/tmp/timings_WXS.tar.gz", + "class": "File" + }, + "run_params": { + "path": "/tmp/params_WXS.params", + "class": "File" + } +} diff --git a/examples/cram_crai.json b/examples/cram_crai.json new file mode 100644 index 0000000..c68a86b --- /dev/null +++ b/examples/cram_crai.json @@ -0,0 +1,43 @@ +{ + "reference": { + "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/core_ref_GRCh37d5.tar.gz", + "class": "File" + }, + "annot": { + "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/VAGrENT_ref_GRCh37d5_ensembl_75.tar.gz", + "class": "File" + }, + "snv_indel": { + "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/SNV_INDEL_ref_GRCh37d5-fragment.tar.gz", + "class": "File" + }, + "tumour": { + "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwxs/COLO-829_ex.cram", + "class": "File" + }, + "tumourIdx": { + "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwxs/COLO-829_ex.cram.crai", + "class": "File" + }, + "normal": { + "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwxs/COLO-829-BL_ex.cram", + "class": "File" + }, + "normalIdx": { + "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwxs/COLO-829-BL_ex.cram.crai", + "class": "File" + }, + "exclude": "NC_007605,hs37d5,GL%", + "result_archive": { + "path": "/tmp/result_WXS.tar.gz", + "class": "File" + }, + "timings": { + "path": "/tmp/timings_WXS.tar.gz", + "class": "File" + }, + "run_params": { + "path": "/tmp/params_WXS.params", + "class": "File" + } +} diff --git a/scripts/analysisWXS.sh b/scripts/analysisWXS.sh index 4236237..d2d14d6 100755 --- a/scripts/analysisWXS.sh +++ b/scripts/analysisWXS.sh @@ -108,13 +108,32 @@ NAME_WT=`samtools view -H $BAM_WT | perl -ne 'if($_ =~ m/^\@RG/) {($sm) = $_ =~m echo -e "\tNAME_MT : $NAME_MT" echo -e "\tNAME_WT : $NAME_WT" -BAM_MT_TMP=$TMP/$NAME_MT.bam -BAM_WT_TMP=$TMP/$NAME_WT.bam +# capture index extension type (assuming same from both) +ALN_EXTN='bam' +BAS_EXTN='bam.bas' +IDX_EXTN='' +if [[ $IDX_MT == *.bam.bai ]] then; + IDX_EXTN='bam.bai' +else if [[ $IDX_MT == *.bam.csi ]] then; + IDX_EXTN='bam.csi' +else if [[ $IDX_MT == *.cram.crai ]] then; + IDX_EXTN='cram.crai' + ALN_EXTN='cram' + BAS_EXTN='cram.bas' +else + echo "Alignment is not BAM or CRAM file: $" >&2 + exit 1 +fi + +BAM_MT_TMP=$TMP/$NAME_MT.$ALN_EXTN +IDX_MT_TMP=$TMP/$NAME_MT.$IDX_EXTN +BAM_WT_TMP=$TMP/$NAME_WT.$ALN_EXTN +IDX_WT_TMP=$TMP/$NAME_WT.$IDX_EXTN ln -fs $BAM_MT $BAM_MT_TMP +ln -fs $IDX_MT $IDX_MT_TMP ln -fs $BAM_WT $BAM_WT_TMP -ln -fs $BAM_MT.bai $BAM_MT_TMP.bai -ln -fs $BAM_WT.bai $BAM_WT_TMP.bai +ln -fs $IDX_WT $IDX_WT_TMP echo "Setting up Parallel block 1" @@ -129,7 +148,7 @@ if [ ! -f "${BAM_WT}.bas" ]; then echo -e "\t[Parallel block 1] BAS $NAME_WT added..." do_parallel[bas_WT]="bam_stats -i $BAM_WT_TMP -o $BAM_WT_TMP.bas" else - ln -fs $BAM_WT.bas $BAM_WT_TMP.bas + ln -fs $BAM_WT.$BAS_EXTN $BAM_WT_TMP.$BAS_EXTN fi echo "Starting Parallel block 1: `date`" diff --git a/scripts/ds-cgpwxs.pl b/scripts/ds-cgpwxs.pl index c1cf16e..f18ebb5 100644 --- a/scripts/ds-cgpwxs.pl +++ b/scripts/ds-cgpwxs.pl @@ -22,7 +22,9 @@ 'a|annot=s' => \$opts{'a'}, 'si|snv_indel=s' => \$opts{'si'}, 't|tumour=s' => \$opts{'t'}, + 'tidx=s' => \$opts{'tidx'}, 'n|normal=s' => \$opts{'n'}, + 'nidx=s' => \$opts{'nidx'}, 'e|exclude=s' => \$opts{'e'}, 'sp|species=s' => \$opts{'sp'}, 'as|assembly=s' => \$opts{'as'}, @@ -105,7 +107,9 @@ printf $FH "OUTPUT_DIR='%s'\n", $ENV{HOME}; printf $FH "REF_BASE='%s'\n", $ref_area; printf $FH "BAM_MT='%s'\n", $opts{'t'}; +printf $FH "IDX_MT='%s'\n", $opts{'tidx'}; printf $FH "BAM_WT='%s'\n", $opts{'n'}; +printf $FH "IDX_WT='%s'\n", $opts{'nidx'}; printf $FH "PINDEL_EXCLUDE='%s'\n", $opts{'e'}; printf $FH "SPECIES='%s'\n", $opts{'sp'}; printf $FH "ASSEMBLY='%s'\n", $opts{'as'}; From ac52358557d4222ee45a5517319304d3e57b0de0 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Thu, 1 Feb 2018 10:38:47 +0000 Subject: [PATCH 16/54] Fixes identifies while testing alternative inputs --- Dockstore.cwl | 2 +- scripts/analysisWXS.sh | 8 ++++---- scripts/ds-cgpwxs.pl | 12 ++---------- 3 files changed, 7 insertions(+), 15 deletions(-) diff --git a/Dockstore.cwl b/Dockstore.cwl index 88abfd3..0686511 100644 --- a/Dockstore.cwl +++ b/Dockstore.cwl @@ -84,7 +84,7 @@ inputs: type: File doc: "Normal alignment file index" inputBinding: - prefix: -normal + prefix: -nidx position: 7 separate: true diff --git a/scripts/analysisWXS.sh b/scripts/analysisWXS.sh index d2d14d6..2daeba4 100755 --- a/scripts/analysisWXS.sh +++ b/scripts/analysisWXS.sh @@ -112,11 +112,11 @@ echo -e "\tNAME_WT : $NAME_WT" ALN_EXTN='bam' BAS_EXTN='bam.bas' IDX_EXTN='' -if [[ $IDX_MT == *.bam.bai ]] then; +if [[ "$IDX_MT" == *.bam.bai ]]; then IDX_EXTN='bam.bai' -else if [[ $IDX_MT == *.bam.csi ]] then; +elif [[ "$IDX_MT" == *.bam.csi ]]; then IDX_EXTN='bam.csi' -else if [[ $IDX_MT == *.cram.crai ]] then; +elif [[ "$IDX_MT" == *.cram.crai ]]; then IDX_EXTN='cram.crai' ALN_EXTN='cram' BAS_EXTN='cram.bas' @@ -141,7 +141,7 @@ if [ ! -f "${BAM_MT}.bas" ]; then echo -e "\t[Parallel block 1] BAS $NAME_MT added..." do_parallel[bas_MT]="bam_stats -i $BAM_MT_TMP -o $BAM_MT_TMP.bas" else - ln -fs $BAM_MT.bas $BAM_MT_TMP.bas + ln -fs $BAM_MT.$BAS_EXTN $BAM_MT_TMP.$BAS_EXTN fi if [ ! -f "${BAM_WT}.bas" ]; then diff --git a/scripts/ds-cgpwxs.pl b/scripts/ds-cgpwxs.pl index f18ebb5..1f7871e 100644 --- a/scripts/ds-cgpwxs.pl +++ b/scripts/ds-cgpwxs.pl @@ -71,16 +71,8 @@ printf "Options loaded: \n%s\n",Dumper(\%opts); -# check all inputs exist and have relevant files (bai/csi/crai + bas) +# check for bas file for my $base_af(($opts{'t'}, $opts{'n'})) { - if($base_af =~ m/\.bam$/) { - # must be bai or csi index - die "ERROR: No index (bai/csi) found for $base_af\n" unless(-e "$base_af.bai" || -e "$base_af.csi"); - } - else { - # cram only have crai as index - die "ERROR: No index (crai) found for $base_af\n" unless(-e "$base_af.crai"); - } warn "WARN: No 'bas' file found for $base_af, this is generated by the mapping flow\n". "\tWill generate internally but suboptimal\n" unless(-e "$base_af.bas"); } @@ -114,7 +106,7 @@ printf $FH "SPECIES='%s'\n", $opts{'sp'}; printf $FH "ASSEMBLY='%s'\n", $opts{'as'}; printf $FH "SNVFLAG='%s'\n", $ini; -printf $FH "CPU=%d\n", $opts{'c'} if(defined $opts{'c'}); +printf $FH "CPU=%d\n", $opts{'c'} if(defined $opts{'c'} && $opts{'c'} > 0); close $FH; exec('analysisWXS.sh'); # I will never return to the perl code From afcaf38bb31b33e47066049b8233c3c886174344 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Mon, 5 Feb 2018 09:24:18 +0000 Subject: [PATCH 17/54] Cleanup for better singularity compatibility --- Dockerfile | 4 +- scripts/analysisWXS.sh | 16 +++-- scripts/ds-cgpwxs.pl | 134 ++++++++++++++++++++++++++--------------- 3 files changed, 99 insertions(+), 55 deletions(-) diff --git a/Dockerfile b/Dockerfile index fc28039..0d63f8b 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM quay.io/wtsicgp/dockstore-cgpmap:3.0.0-rc2 as builder +FROM quay.io/wtsicgp/dockstore-cgpmap:3.0.0-rc5 as builder USER root @@ -6,6 +6,7 @@ ENV OPT /opt/wtsi-cgp ENV PATH $OPT/bin:$PATH ENV PERL5LIB $OPT/lib/perl5 ENV LD_LIBRARY_PATH $OPT/lib +ENV LC_ALL C RUN apt-get -yq update RUN apt-get install -yq --no-install-recommends\ @@ -38,6 +39,7 @@ ENV OPT /opt/wtsi-cgp ENV PATH $OPT/bin:$PATH ENV PERL5LIB $OPT/lib/perl5 ENV LD_LIBRARY_PATH $OPT/lib +ENV LC_ALL C RUN apt-get -yq update RUN apt-get install -yq --no-install-recommends\ diff --git a/scripts/analysisWXS.sh b/scripts/analysisWXS.sh index 2daeba4..c524bbe 100755 --- a/scripts/analysisWXS.sh +++ b/scripts/analysisWXS.sh @@ -52,9 +52,12 @@ echo -e "\nStart workflow: `date`\n" declare -a PRE_EXEC declare -a POST_EXEC -if [ -z ${PARAM_FILE+x} ] ; then +if [[ $# -eq 1 ]] ; then + PARAM_FILE=$1 +elif [ -z ${PARAM_FILE+x} ] ; then PARAM_FILE=$HOME/run.params fi + echo "Loading user options from: $PARAM_FILE" if [ ! -f $PARAM_FILE ]; then echo -e "\tERROR: file indicated by PARAM_FILE not found: $PARAM_FILE" 1>&2 @@ -110,7 +113,6 @@ echo -e "\tNAME_WT : $NAME_WT" # capture index extension type (assuming same from both) ALN_EXTN='bam' -BAS_EXTN='bam.bas' IDX_EXTN='' if [[ "$IDX_MT" == *.bam.bai ]]; then IDX_EXTN='bam.bai' @@ -119,7 +121,6 @@ elif [[ "$IDX_MT" == *.bam.csi ]]; then elif [[ "$IDX_MT" == *.cram.crai ]]; then IDX_EXTN='cram.crai' ALN_EXTN='cram' - BAS_EXTN='cram.bas' else echo "Alignment is not BAM or CRAM file: $" >&2 exit 1 @@ -141,14 +142,14 @@ if [ ! -f "${BAM_MT}.bas" ]; then echo -e "\t[Parallel block 1] BAS $NAME_MT added..." do_parallel[bas_MT]="bam_stats -i $BAM_MT_TMP -o $BAM_MT_TMP.bas" else - ln -fs $BAM_MT.$BAS_EXTN $BAM_MT_TMP.$BAS_EXTN + ln -fs $BAM_MT.$BAS_EXTN $BAM_MT_TMP.'.bas' fi if [ ! -f "${BAM_WT}.bas" ]; then echo -e "\t[Parallel block 1] BAS $NAME_WT added..." do_parallel[bas_WT]="bam_stats -i $BAM_WT_TMP -o $BAM_WT_TMP.bas" else - ln -fs $BAM_WT.$BAS_EXTN $BAM_WT_TMP.$BAS_EXTN + ln -fs $BAM_WT.$BAS_EXTN $BAM_WT_TMP.'.bas' fi echo "Starting Parallel block 1: `date`" @@ -233,6 +234,11 @@ run_parallel $CPU do_parallel # clean up log files rm -rf $OUTPUT_DIR/${NAME_MT}_vs_${NAME_WT}/*/logs +# cleanup reference area, see ds-cgpwxs.pl +if [ ! -z ${CLEAN_REF+x} ]; then + rm -rf $REF_BASE +fi + echo 'Package results' # timings first tar -C $OUTPUT_DIR -zcf ${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.timings.tar.gz timings diff --git a/scripts/ds-cgpwxs.pl b/scripts/ds-cgpwxs.pl index 1f7871e..c4cc46c 100644 --- a/scripts/ds-cgpwxs.pl +++ b/scripts/ds-cgpwxs.pl @@ -11,10 +11,11 @@ pod2usage(-verbose => 1, -exitval => 1) if(@ARGV == 0); # set defaults -my %opts = ('c'=>0, - 'sc' => q{}, - 'b' => q{} - ,); +my %opts = ('c' => undef, + 'o' => $ENV{HOME}, + 'sp' => undef, + 'as' => undef, + ); GetOptions( 'h|help' => \$opts{'h'}, 'm|man' => \$opts{'m'}, @@ -26,10 +27,10 @@ 'n|normal=s' => \$opts{'n'}, 'nidx=s' => \$opts{'nidx'}, 'e|exclude=s' => \$opts{'e'}, - 'sp|species=s' => \$opts{'sp'}, - 'as|assembly=s' => \$opts{'as'}, - 'sf|snvflag=s' => \$opts{'sf'}, + 'sp|species:s' => \$opts{'sp'}, + 'as|assembly:s' => \$opts{'as'}, 'c|cores:i' => \$opts{'c'}, + 'o|outdir:s' => \$opts{'o'}, ) or pod2usage(2); pod2usage(-verbose => 1, -exitval => 0) if(defined $opts{'h'}); @@ -37,37 +38,8 @@ delete $opts{'h'}; delete $opts{'m'}; -delete $opts{'sp'} if(! defined $opts{'sp'} || length $opts{'sp'} == 0); -delete $opts{'as'} if(! defined $opts{'as'} || length $opts{'as'} == 0); - -## read species/assembly from bam headers -my ($mt_species, $mt_assembly) = species_assembly_from_xam($opts{'t'}); -my ($wt_species, $wt_assembly) = species_assembly_from_xam($opts{'n'}); -if($mt_species ne $wt_species) { - warn "WARN: Species mismatch between T/N [CR|B]AM headers\n"; - if(!defined $opts{'sp'}) { - die "ERROR: Please define species to handle this mismatch\n"; - } -} -elsif($mt_species ne q{}) { - $opts{'sp'} = $mt_species; -} -if(!defined $opts{'sp'}) { - die "ERROR: Please define species, not found in [CR|B]AM headers.\n"; -} -if($mt_assembly ne $wt_assembly) { - warn "WARN: Assembly mismatch between T/N [CR|B]AM headers\n"; - if(!defined $opts{'as'}) { - die "ERROR: Please define assembly to handle this mismatch\n"; - } -} -elsif($mt_assembly ne q{}) { - $opts{'as'} = $mt_assembly; -} -if(!defined $opts{'as'}) { - die "ERROR: Please define assembly, not found in [CR|B]AM headers.\n"; -} +resolve_sp_as(\%opts); printf "Options loaded: \n%s\n",Dumper(\%opts); @@ -77,16 +49,24 @@ "\tWill generate internally but suboptimal\n" unless(-e "$base_af.bas"); } -## unpack the reference area: -my $ref_area = $ENV{HOME}.'/reference_files'; - -ref_unpack($ref_area, $opts{'r'}); -ref_unpack($ref_area, $opts{'a'}); -ref_unpack($ref_area, $opts{'si'}); +# figure out if ref already unpacked: +my $ref_area = $opts{'o'}.'/reference_files'; +my $ref_unpack = 1; +if($opts{'r'} eq $opts{'a'} && $opts{'r'} eq $opts{'si'} && -d $opts{'r'}) { + $ref_area = $opts{'r'}; + $ref_unpack = 0; +} +else { + ref_unpack($ref_area, $opts{'r'}); + ref_unpack($ref_area, $opts{'a'}); + ref_unpack($ref_area, $opts{'si'}); +} ## now complete the caveman flaging file correctly -my $ini = add_species_flag_ini($opts{'sp'}, "$ref_area/caveman/flag.vcf.config.WXS.ini"); +my $ini = add_species_flag_ini($opts{'sp'}, $ref_area.'/caveman/flag.vcf.config.WXS.ini', $opts{'o'}); +# make the param file +make_path($opts{'o'}) unless(-e $opts{'o'}); my $run_file = $ENV{HOME}.'/run.params'; open my $FH,'>',$run_file or die "Failed to write to $run_file: $!"; # Force explicit checking of file flush @@ -96,7 +76,7 @@ # hard-coded printf $FH "PROTOCOL=WXS\n"; # required options -printf $FH "OUTPUT_DIR='%s'\n", $ENV{HOME}; +printf $FH "OUTPUT_DIR='%s'\n", $opts{'o'}; printf $FH "REF_BASE='%s'\n", $ref_area; printf $FH "BAM_MT='%s'\n", $opts{'t'}; printf $FH "IDX_MT='%s'\n", $opts{'tidx'}; @@ -106,16 +86,49 @@ printf $FH "SPECIES='%s'\n", $opts{'sp'}; printf $FH "ASSEMBLY='%s'\n", $opts{'as'}; printf $FH "SNVFLAG='%s'\n", $ini; -printf $FH "CPU=%d\n", $opts{'c'} if(defined $opts{'c'} && $opts{'c'} > 0); +printf $FH "CPU=%d\n", $opts{'c'} if(defined $opts{'c'}); +printf $FH "CLEAN_REF=1\n" if($ref_unpack); close $FH; -exec('analysisWXS.sh'); # I will never return to the perl code +exec('analysisWXS.sh', $run_file); # I will never return to the perl code + +sub resolve_sp_as { + my $options = shift; + ## read species/assembly from bam headers + my ($mt_species, $mt_assembly) = species_assembly_from_xam($options->{'t'}); + my ($wt_species, $wt_assembly) = species_assembly_from_xam($options->{'n'}); + if($mt_species ne $wt_species) { + warn "WARN: Species mismatch between T/N [CR|B]AM headers\n"; + if(!defined $options->{'sp'}) { + die "ERROR: Please define species to handle this mismatch\n"; + } + } + elsif($mt_species ne q{}) { + $options->{'sp'} = $mt_species; + } + if(!defined $options->{'sp'}) { + die "ERROR: Please define species, not found in [CR|B]AM headers.\n"; + } + + if($mt_assembly ne $wt_assembly) { + warn "WARN: Assembly mismatch between T/N [CR|B]AM headers\n"; + if(!defined $options->{'as'}) { + die "ERROR: Please define assembly to handle this mismatch\n"; + } + } + elsif($mt_assembly ne q{}) { + $options->{'as'} = $mt_assembly; + } + if(!defined $options->{'as'}) { + die "ERROR: Please define assembly, not found in [CR|B]AM headers.\n"; + } +} sub add_species_flag_ini { - my ($species, $ini_in) = @_; + my ($species, $ini_in, $outdir) = @_; $species =~ s/ /_/g; $species = uc $species; - my $ini_out = $ENV{HOME}.'/flag.vcf.config.WXS.ini'; + my $ini_out = $outdir.'/flag.vcf.config.WXS.ini'; open my $IN, '<', $ini_in; open my $OUT,'>',$ini_out; while(my $line = <$IN>) { @@ -187,13 +200,17 @@ =head1 SYNOPSIS -annot -a Path to VAGrENT*.tar.gz -snv_indel -si Path to SNV_INDEL*.tar.gz -tumour -t Tumour [CR|B]AM file + -tidx Tumour index file -normal -n Normal [CR|B]AM file + -nidx Normal index file -exclude -e Exclude these contigs from pindel analysis e.g. NC_007605,hs37d5,GL% Optional parameters -species -sp Species name (may require quoting) -assembly -a Reference assembly + -outdir -o Set the output folder [$HOME] + -cores -c Set the number of cpu/cores available [default all]. Other: -help -h Brief help message. @@ -223,10 +240,18 @@ =head1 OPTION DETAILS Path to tumour BAM or CRAM file with co-located index and BAS file. +=item B<-tidx> + +Path to tumour alignment file index (bai|csi|crai), still needs to be colocated. + =item B<-normal> Path to normal BAM or CRAM file with co-located index and BAS file. +=item B<-nidx> + +Path to normal alignment file index (bai|csi|crai), still needs to be colocated. + =item B<-exclude> Contigs to be excluded from Pindel analysis, csv, use '%' for wildcard. @@ -241,6 +266,17 @@ =head1 OPTION DETAILS Specify overriding assembly, by default will select the most prevelant entry in [CR|B]AM header (to cope with inclusion of viral/decoy sequences). +=item B<-outdir> + +Set the output directory. Defaults to $HOME. + +NOTE: Should B be set when working with dockstore wrapper. + +=item B<-cores> + +Sets the number of cores to be used during processing. Default to use all at appropriate +points in analysis. + =back =cut From 23e5b717370a5ec803de5cb91f6c9dd5e5f88a09 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 7 Feb 2018 16:17:56 +0000 Subject: [PATCH 18/54] Move cwl and examples to enable multi cwl if needed --- Dockstore.cwl => cwls/cgpwxs.cwl | 33 ++++++++++++++++++++++------ examples/{ => cgpwxs}/bam_bai.json | 0 examples/{ => cgpwxs}/bam_csi.json | 0 examples/{ => cgpwxs}/cram_crai.json | 0 4 files changed, 26 insertions(+), 7 deletions(-) rename Dockstore.cwl => cwls/cgpwxs.cwl (72%) rename examples/{ => cgpwxs}/bam_bai.json (100%) rename examples/{ => cgpwxs}/bam_csi.json (100%) rename examples/{ => cgpwxs}/cram_crai.json (100%) diff --git a/Dockstore.cwl b/cwls/cgpwxs.cwl similarity index 72% rename from Dockstore.cwl rename to cwls/cgpwxs.cwl index 0686511..f079dc8 100644 --- a/Dockstore.cwl +++ b/cwls/cgpwxs.cwl @@ -9,17 +9,16 @@ label: "CGP WXS analysis flow" cwlVersion: v1.0 doc: | - ![build_status](https://quay.io/repository/wtsicgp/dockstore-cgpwxs/status) - A Docker container for the CGP WXS analysis flow. See the [dockstore-cgpwxs](https://github.com/cancerit/dockstore-cgpwxs) website for more information. + ![build_status](https://quay.io/repository/wtsicgp/dockstore-cgpwxs/status) + A Docker container for the CGP WXS analysis flow. See the [dockstore-cgpwxs](https://github.com/cancerit/dockstore-cgpwxs) website for more information. -dct:creator: - "@id": "http://orcid.org/0000-0002-5634-1539" - foaf:name: Keiran M Raine - foaf:mbox: "keiranmraine@gmail.com" + Parameters for a CWL definition are generally described in a json file, but parameters can be provided on the command line. + + To see the parameters descriptions please run: cwltool --tool-help path_to.cwl requirements: - class: DockerRequirement - dockerPull: "quay.io/wtsicgp/dockstore-cgpwxs:3.0.0-rc1" + dockerPull: "quay.io/wtsicgp/dockstore-cgpwxs:3.0.0-rc2" hints: - class: ResourceRequirement @@ -135,3 +134,23 @@ outputs: glob: WXS_*_vs_*.timings.tar.gz baseCommand: ["/opt/wtsi-cgp/bin/ds-cgpwxs.pl"] + +$schemas: + - http://schema.org/docs/schema_org_rdfa.html + +$namespaces: + s: http://schema.org/ + +s:codeRepository: https://github.com/cancerit/dockstore-cgpwxs +s:license: https://spdx.org/licenses/AGPL-3.0-only + +s:author: + - class: s:Person + s:identifier: https://orcid.org/0000-0002-5634-1539 + s:email: mailto:keiranmraine@gmail.com + s:name: Keiran Raine + +dct:creator: + "@id": "keiranmraine@gmail.com" + foaf:name: Keiran Raine + foaf:mbox: "keiranmraine@gmail.com" diff --git a/examples/bam_bai.json b/examples/cgpwxs/bam_bai.json similarity index 100% rename from examples/bam_bai.json rename to examples/cgpwxs/bam_bai.json diff --git a/examples/bam_csi.json b/examples/cgpwxs/bam_csi.json similarity index 100% rename from examples/bam_csi.json rename to examples/cgpwxs/bam_csi.json diff --git a/examples/cram_crai.json b/examples/cgpwxs/cram_crai.json similarity index 100% rename from examples/cram_crai.json rename to examples/cgpwxs/cram_crai.json From 7ca83bc086830da28f316fa863182e0c9e6c0fb3 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 7 Feb 2018 16:18:44 +0000 Subject: [PATCH 19/54] Improves build stage settings --- Dockerfile | 51 ++++++++++++++++++++++++++++++--------------------- 1 file changed, 30 insertions(+), 21 deletions(-) diff --git a/Dockerfile b/Dockerfile index 0d63f8b..a9d731a 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,15 +1,10 @@ -FROM quay.io/wtsicgp/dockstore-cgpmap:3.0.0-rc5 as builder +FROM quay.io/wtsicgp/dockstore-cgpmap:3.0.0-rc8 as builder USER root -ENV OPT /opt/wtsi-cgp -ENV PATH $OPT/bin:$PATH -ENV PERL5LIB $OPT/lib/perl5 -ENV LD_LIBRARY_PATH $OPT/lib -ENV LC_ALL C - RUN apt-get -yq update RUN apt-get install -yq --no-install-recommends\ + locales\ g++\ make\ gcc\ @@ -19,31 +14,31 @@ RUN apt-get install -yq --no-install-recommends\ # python only for building bedtools, not needed to use -ADD build/opt-build.sh build/ -RUN bash build/opt-build.sh $OPT +RUN locale-gen en_US.UTF-8 +RUN update-locale LANG=en_US.UTF-8 -ADD scripts/analysisWXS.sh $OPT/bin/analysisWXS.sh -ADD scripts/ds-cgpwxs.pl $OPT/bin/ds-cgpwxs.pl -RUN chmod a+x $OPT/bin/analysisWXS.sh $OPT/bin/ds-cgpwxs.pl +ENV OPT /opt/wtsi-cgp +ENV PATH $OPT/bin:$PATH +ENV PERL5LIB $OPT/lib/perl5 +ENV LD_LIBRARY_PATH $OPT/lib +ENV LC_ALL en_US.UTF-8 +ENV LANG en_US.UTF-8 +ADD build/opt-build.sh build/ +RUN bash build/opt-build.sh $OPT FROM ubuntu:16.04 MAINTAINER keiranmraine@gmail.com -LABEL uk.ac.sanger.cgp="Cancer Genome Project, Wellcome Trust Sanger Institute" \ - version="3.0.0-rc1" \ - description="The CGP WXS pipeline for dockstore.org" - -ENV OPT /opt/wtsi-cgp -ENV PATH $OPT/bin:$PATH -ENV PERL5LIB $OPT/lib/perl5 -ENV LD_LIBRARY_PATH $OPT/lib -ENV LC_ALL C +LABEL vendor="Cancer Genome Project, Wellcome Trust Sanger Institute" +LABEL uk.ac.sanger.cgp.description="CGP WXS pipeline for dockstore.org" +LABEL uk.ac.sanger.cgp.version="3.0.0-rc1" RUN apt-get -yq update RUN apt-get install -yq --no-install-recommends\ apt-transport-https\ + locales\ curl\ ca-certificates\ libperlio-gzip-perl\ @@ -54,9 +49,23 @@ RUN apt-get install -yq --no-install-recommends\ liblzma5\ libncurses5 +RUN locale-gen en_US.UTF-8 +RUN update-locale LANG=en_US.UTF-8 + +ENV OPT /opt/wtsi-cgp +ENV PATH $OPT/bin:$PATH +ENV PERL5LIB $OPT/lib/perl5 +ENV LD_LIBRARY_PATH $OPT/lib +ENV LC_ALL en_US.UTF-8 +ENV LANG en_US.UTF-8 + RUN mkdir -p $OPT COPY --from=builder $OPT $OPT +ADD scripts/analysisWXS.sh $OPT/bin/analysisWXS.sh +ADD scripts/ds-cgpwxs.pl $OPT/bin/ds-cgpwxs.pl +RUN chmod a+x $OPT/bin/analysisWXS.sh $OPT/bin/ds-cgpwxs.pl + ## USER CONFIGURATION RUN adduser --disabled-password --gecos '' ubuntu && chsh -s /bin/bash && mkdir -p /home/ubuntu From de71a3663122c36dce0834895db6251d706aa623 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 7 Feb 2018 16:19:13 +0000 Subject: [PATCH 20/54] Remove pre/post exec and mention in changes --- CHANGES.md | 1 + scripts/analysisWXS.sh | 29 ----------------------------- 2 files changed, 1 insertion(+), 29 deletions(-) diff --git a/CHANGES.md b/CHANGES.md index 3e4e70c..9782fc3 100644 --- a/CHANGES.md +++ b/CHANGES.md @@ -5,6 +5,7 @@ * See dockstore-cgpmap v3.0.0 (primarily adds mismatchQc) * CaVEMan and cgpPindel updated to use freagment based counting. * Using build stages to shrink images. +* remove legacy PRE/POST-EXEC from cgpbox days ## 2.1.0 diff --git a/scripts/analysisWXS.sh b/scripts/analysisWXS.sh index c524bbe..e73ca8d 100755 --- a/scripts/analysisWXS.sh +++ b/scripts/analysisWXS.sh @@ -80,30 +80,9 @@ fi echo -e "\tBAM_MT : $BAM_MT" echo -e "\tBAM_WT : $BAM_WT" -if [ ${#PRE_EXEC[@]} -eq 0 ]; then - PRE_EXEC='echo No PRE_EXEC defined' -fi - -if [ ${#POST_EXEC[@]} -eq 0 ]; then - POST_EXEC='echo No POST_EXEC defined' -fi - set -u mkdir -p $OUTPUT_DIR -# run any pre-exec step before attempting to access BAMs -# logically the pre-exec could be pulling them -if [ ! -f $OUTPUT_DIR/pre-exec.done ]; then - echo -e "\nRun PRE_EXEC: `date`" - - for i in "${PRE_EXEC[@]}"; do - set -x - $i - { set +x; } 2> /dev/null - done - touch $OUTPUT_DIR/pre-exec.done -fi - ## get sample names from BAM headers NAME_MT=`samtools view -H $BAM_MT | perl -ne 'if($_ =~ m/^\@RG/) {($sm) = $_ =~m/\tSM:([^\t]+)/; print "$sm\n";}' | uniq` NAME_WT=`samtools view -H $BAM_WT | perl -ne 'if($_ =~ m/^\@RG/) {($sm) = $_ =~m/\tSM:([^\t]+)/; print "$sm\n";}' | uniq` @@ -245,12 +224,4 @@ tar -C $OUTPUT_DIR -zcf ${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.timings.tar.gz timi tar -C $OUTPUT_DIR -zcf ${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.result.tar.gz ${NAME_MT}_vs_${NAME_WT} cp $PARAM_FILE ${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.run.params -# run any post-exec step -echo -e "\nRun POST_EXEC: `date`" -for i in "${POST_EXEC[@]}"; do - set -x - $i - set +x -done - echo -e "\nWorkflow end: `date`" From 284d98a5b86d311b0122cf7f3f233d358335b845 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Thu, 8 Feb 2018 13:38:19 +0000 Subject: [PATCH 21/54] Maintain backwards compat --- Dockstore.cwl | 1 + 1 file changed, 1 insertion(+) create mode 120000 Dockstore.cwl diff --git a/Dockstore.cwl b/Dockstore.cwl new file mode 120000 index 0000000..9976871 --- /dev/null +++ b/Dockstore.cwl @@ -0,0 +1 @@ +cwls/cgpwxs.cwl \ No newline at end of file From e57919d90b0e42bdeb03cb7acda4ba37ea730469 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Thu, 8 Feb 2018 13:50:09 +0000 Subject: [PATCH 22/54] cleanup docker context --- .dockerignore | 1 + 1 file changed, 1 insertion(+) diff --git a/.dockerignore b/.dockerignore index b83d44d..db9a224 100644 --- a/.dockerignore +++ b/.dockerignore @@ -1,4 +1,5 @@ /examples/ +/cwls/ /git-hooks/ /*.* /LICENSE From 6fb1cbc5c9b8800b01ecdbb52c04402d7f75f018 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Thu, 8 Feb 2018 13:50:48 +0000 Subject: [PATCH 23/54] Make examples run as fast as possible by excluding contigs with no data --- examples/cgpwxs/bam_bai.json | 2 +- examples/cgpwxs/bam_csi.json | 2 +- examples/cgpwxs/cram_crai.json | 2 +- 3 files changed, 3 insertions(+), 3 deletions(-) diff --git a/examples/cgpwxs/bam_bai.json b/examples/cgpwxs/bam_bai.json index 5206b71..f74507f 100644 --- a/examples/cgpwxs/bam_bai.json +++ b/examples/cgpwxs/bam_bai.json @@ -27,7 +27,7 @@ "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwxs/COLO-829-BL_ex.bam.bai", "class": "File" }, - "exclude": "NC_007605,hs37d5,GL%", + "exclude": "1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,22,X,Y,MT,NC_007605,hs37d5,GL%", "result_archive": { "path": "/tmp/result_WXS.tar.gz", "class": "File" diff --git a/examples/cgpwxs/bam_csi.json b/examples/cgpwxs/bam_csi.json index b5e7d93..647ae02 100644 --- a/examples/cgpwxs/bam_csi.json +++ b/examples/cgpwxs/bam_csi.json @@ -27,7 +27,7 @@ "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwxs/COLO-829-BL_ex.bam.csi", "class": "File" }, - "exclude": "NC_007605,hs37d5,GL%", + "exclude": "1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,22,X,Y,MT,NC_007605,hs37d5,GL%", "result_archive": { "path": "/tmp/result_WXS.tar.gz", "class": "File" diff --git a/examples/cgpwxs/cram_crai.json b/examples/cgpwxs/cram_crai.json index c68a86b..4b5a884 100644 --- a/examples/cgpwxs/cram_crai.json +++ b/examples/cgpwxs/cram_crai.json @@ -27,7 +27,7 @@ "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwxs/COLO-829-BL_ex.cram.crai", "class": "File" }, - "exclude": "NC_007605,hs37d5,GL%", + "exclude": "1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,22,X,Y,MT,NC_007605,hs37d5,GL%", "result_archive": { "path": "/tmp/result_WXS.tar.gz", "class": "File" From f7b4668cf805c82efab419251a092a7c14922221 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Thu, 8 Feb 2018 13:51:06 +0000 Subject: [PATCH 24/54] Correct linking of bas files --- scripts/analysisWXS.sh | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/scripts/analysisWXS.sh b/scripts/analysisWXS.sh index e73ca8d..dc74837 100755 --- a/scripts/analysisWXS.sh +++ b/scripts/analysisWXS.sh @@ -121,14 +121,14 @@ if [ ! -f "${BAM_MT}.bas" ]; then echo -e "\t[Parallel block 1] BAS $NAME_MT added..." do_parallel[bas_MT]="bam_stats -i $BAM_MT_TMP -o $BAM_MT_TMP.bas" else - ln -fs $BAM_MT.$BAS_EXTN $BAM_MT_TMP.'.bas' + ln -fs $BAM_MT.bas $BAM_MT_TMP.bas fi if [ ! -f "${BAM_WT}.bas" ]; then echo -e "\t[Parallel block 1] BAS $NAME_WT added..." do_parallel[bas_WT]="bam_stats -i $BAM_WT_TMP -o $BAM_WT_TMP.bas" else - ln -fs $BAM_WT.$BAS_EXTN $BAM_WT_TMP.'.bas' + ln -fs $BAM_WT.bas $BAM_WT_TMP.bas fi echo "Starting Parallel block 1: `date`" From 45f00f76d4712692959fd492d56377e2f9477a62 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Fri, 9 Feb 2018 15:35:11 +0000 Subject: [PATCH 25/54] Doc in cwl file [ci skip] --- cwls/cgpwxs.cwl | 3 +++ 1 file changed, 3 insertions(+) diff --git a/cwls/cgpwxs.cwl b/cwls/cgpwxs.cwl index f079dc8..ae1f3af 100644 --- a/cwls/cgpwxs.cwl +++ b/cwls/cgpwxs.cwl @@ -16,6 +16,9 @@ doc: | To see the parameters descriptions please run: cwltool --tool-help path_to.cwl + WARNING: The usual setting for 'exclude' is 'NC_007605,hs37d5,GL%' (human GRCh37/NCBI37). Examples + are configured to run as quickly as possible. + requirements: - class: DockerRequirement dockerPull: "quay.io/wtsicgp/dockstore-cgpwxs:3.0.0-rc2" From 16571334a49037b642c5cd3c5f5123e9366b267b Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Fri, 9 Feb 2018 17:24:23 +0000 Subject: [PATCH 26/54] Use exclude with caveman too --- scripts/analysisWXS.sh | 5 +++-- scripts/ds-cgpwxs.pl | 6 +++--- 2 files changed, 6 insertions(+), 5 deletions(-) diff --git a/scripts/analysisWXS.sh b/scripts/analysisWXS.sh index dc74837..d422296 100755 --- a/scripts/analysisWXS.sh +++ b/scripts/analysisWXS.sh @@ -153,7 +153,7 @@ do_parallel[cgpPindel]="pindel.pl \ -st $PROTOCOL \ -as $ASSEMBLY \ -sp $SPECIES \ - -e $PINDEL_EXCLUDE \ + -e $CONTIG_EXCLUDE \ -b $REF_BASE/pindel/HiDepth.bed.gz \ -c $CPU \ -sf $REF_BASE/pindel/softRules.lst" @@ -184,7 +184,8 @@ do_parallel[CaVEMan]="caveman.pl \ -f $REF_BASE/caveman/flagging/flag.to.vcf.convert.ini \ -o $OUTPUT_DIR/${NAME_MT}_vs_${NAME_WT}/caveman \ -np $PROTOCOL \ - -tp $PROTOCOL" + -tp $PROTOCOL \ + -x $CONTIG_EXCLUDE" echo "Starting Parallel block 2: `date`" run_parallel $CPU do_parallel diff --git a/scripts/ds-cgpwxs.pl b/scripts/ds-cgpwxs.pl index c4cc46c..0993c38 100644 --- a/scripts/ds-cgpwxs.pl +++ b/scripts/ds-cgpwxs.pl @@ -82,7 +82,7 @@ printf $FH "IDX_MT='%s'\n", $opts{'tidx'}; printf $FH "BAM_WT='%s'\n", $opts{'n'}; printf $FH "IDX_WT='%s'\n", $opts{'nidx'}; -printf $FH "PINDEL_EXCLUDE='%s'\n", $opts{'e'}; +printf $FH "CONTIG_EXCLUDE='%s'\n", $opts{'e'}; printf $FH "SPECIES='%s'\n", $opts{'sp'}; printf $FH "ASSEMBLY='%s'\n", $opts{'as'}; printf $FH "SNVFLAG='%s'\n", $ini; @@ -203,7 +203,7 @@ =head1 SYNOPSIS -tidx Tumour index file -normal -n Normal [CR|B]AM file -nidx Normal index file - -exclude -e Exclude these contigs from pindel analysis + -exclude -e Exclude these contigs from analysis e.g. NC_007605,hs37d5,GL% Optional parameters @@ -254,7 +254,7 @@ =head1 OPTION DETAILS =item B<-exclude> -Contigs to be excluded from Pindel analysis, csv, use '%' for wildcard. +Contigs to be excluded from analysis, csv, use '%' for wildcard. =item B<-species> From 072d82f1f731eee087e27598ba7176dd5dc9e707 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Mon, 12 Feb 2018 13:28:57 +0000 Subject: [PATCH 27/54] Handle inadequacy of Bio::DB::HTS by creating a local cache [ci skip] --- scripts/analysisWXS.sh | 9 +++++++++ 1 file changed, 9 insertions(+) diff --git a/scripts/analysisWXS.sh b/scripts/analysisWXS.sh index d422296..a994be9 100755 --- a/scripts/analysisWXS.sh +++ b/scripts/analysisWXS.sh @@ -131,6 +131,12 @@ else ln -fs $BAM_WT.bas $BAM_WT_TMP.bas fi +## prime the cache +USER_CACHE=$OUTPUT_DIR/ref_cache +export REF_CACHE=$USER_CACHE/%2s/%2s/%s +export REF_PATH=$REF_CACHE:http://www.ebi.ac.uk/ena/cram/md5/%s +do_parallel[cache_POP]="seq_cache_populate.pl -root $USER_CACHE $REF_BASE/genome.fa" + echo "Starting Parallel block 1: `date`" run_parallel $CPU do_parallel @@ -219,6 +225,9 @@ if [ ! -z ${CLEAN_REF+x} ]; then rm -rf $REF_BASE fi +# cleanup ref cache +rm -rf $USER_CACHE + echo 'Package results' # timings first tar -C $OUTPUT_DIR -zcf ${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.timings.tar.gz timings From 6f2ada0781b9774398fdb213e4f75150438b8cfe Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Mon, 12 Feb 2018 16:01:07 +0000 Subject: [PATCH 28/54] Change to feature branches until testing completed [ci skip] --- build/opt-build.sh | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/build/opt-build.sh b/build/opt-build.sh index 930e25b..560ed34 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -13,15 +13,15 @@ VER_CGPVCF="v2.2.1" VER_VCFTOOLS="0.1.15" # cgpPindel -VER_CGPPINDEL="v3.0.0-rc1" +VER_CGPPINDEL="feature/readsVsFragments" # cgpCaVEManPostProcessing -VER_CGPCAVEPOSTPROC="1.8.0-rc1" +VER_CGPCAVEPOSTPROC="feature/overlapping_reads" # if issues found downgrade to 2.23.0 but can't find any use of bedtools coverage VER_BEDTOOLS="2.27.1" # cgpCaVEManWrapper -VER_CGPCAVEWRAP="1.13.0-rc1" +VER_CGPCAVEWRAP="feature/overlapping_reads" VER_CAVEMAN="1.13.0-rc1" # VAGrENT From 5c0cf63c8163209eeefd46ed650d15f0b7bdbcd2 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Sun, 18 Feb 2018 11:34:28 +0000 Subject: [PATCH 29/54] More cleanup --- CHANGES.md | 4 ++- Dockerfile | 7 +++-- README.md | 64 +++++++++++++++++++++++++++++++++--------- build/apt-build.sh | 5 ---- cwls/cgpwxs.cwl | 3 ++ scripts/analysisWXS.sh | 15 +++++----- scripts/ds-cgpwxs.pl | 3 +- 7 files changed, 69 insertions(+), 32 deletions(-) delete mode 100644 build/apt-build.sh diff --git a/CHANGES.md b/CHANGES.md index 9782fc3..b69aa03 100644 --- a/CHANGES.md +++ b/CHANGES.md @@ -3,7 +3,9 @@ ## NEXT * See dockstore-cgpmap v3.0.0 (primarily adds mismatchQc) -* CaVEMan and cgpPindel updated to use freagment based counting. +* CaVEMan and cgpPindel updated to use fragment based counting. + * You will need to update the reference pack to include the new flagging rules, see example `json` + files * Using build stages to shrink images. * remove legacy PRE/POST-EXEC from cgpbox days diff --git a/Dockerfile b/Dockerfile index a9d731a..f56ea43 100644 --- a/Dockerfile +++ b/Dockerfile @@ -18,7 +18,7 @@ RUN locale-gen en_US.UTF-8 RUN update-locale LANG=en_US.UTF-8 ENV OPT /opt/wtsi-cgp -ENV PATH $OPT/bin:$PATH +ENV PATH $OPT/bin:$OPT/biobambam2/bin:$PATH ENV PERL5LIB $OPT/lib/perl5 ENV LD_LIBRARY_PATH $OPT/lib ENV LC_ALL en_US.UTF-8 @@ -47,13 +47,14 @@ RUN apt-get install -yq --no-install-recommends\ time\ zlib1g\ liblzma5\ - libncurses5 + libncurses5\ + p11-kit RUN locale-gen en_US.UTF-8 RUN update-locale LANG=en_US.UTF-8 ENV OPT /opt/wtsi-cgp -ENV PATH $OPT/bin:$PATH +ENV PATH $OPT/bin:$OPT/biobambam2/bin:$PATH ENV PERL5LIB $OPT/lib/perl5 ENV LD_LIBRARY_PATH $OPT/lib ENV LC_ALL en_US.UTF-8 diff --git a/README.md b/README.md index 249caab..8e89b58 100644 --- a/README.md +++ b/README.md @@ -1,11 +1,16 @@ # dockstore-cgpwxs -`dockstore-cgpwxs` provides a complete multi threaded WXS analysis for SNV and INDEL variants with associated annotation of VCF files. This has been packaged specifically for use with the [Dockstore.org](https://dockstore.org/) framework. +`dockstore-cgpwxs` provides a complete multi threaded WXS analysis for SNV and INDEL variants with +associated annotation of VCF files. This has been packaged specifically for use with the +[Dockstore.org](https://dockstore.org/) framework. -[![Docker Repository on Quay](https://quay.io/repository/wtsicgp/dockstore-cgpwxs/status "Docker Repository on Quay")](https://quay.io/repository/wtsicgp/dockstore-cgpwxs) +[![Gitter Badge][gitter-svg]][gitter-badge] -[![Build Status](https://travis-ci.org/cancerit/dockstore-cgpwxs.svg?branch=master)](https://travis-ci.org/cancerit/dockstore-cgpwxs) : master -[![Build Status](https://travis-ci.org/cancerit/dockstore-cgpwxs.svg?branch=develop)](https://travis-ci.org/cancerit/dockstore-cgpwxs) : develop +[![Quay Badge][quay-status]][quay-repo] + +| Master | Develop | +| --------------------------------------------- | ----------------------------------------------- | +| [![Master Badge][travis-master]][travis-base] | [![Develop Badge][travis-develop]][travis-base] | Required input files are @@ -14,23 +19,29 @@ will allow use of these. 1. Tumour BAM/CRAM file (plus bai/csi/crai + bas) 1. Normal BAM/CRAM file (plus bai/csi/crai + bas) -1. Core reference archive (e.g. [core_ref_GRCh37d5.tar.gz](ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/)) -1. WXS reference archive (e.g. [SNV_INDEL_ref_GRCh37d5.tar.gz](ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/)) -1. VAGrENT (annotation) reference archive (e.g. [VAGrENT_ref_GRCh37d5_ensembl_75.tar.gz](ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/)) +1. Core reference archive (e.g. [core_ref_GRCh37d5.tar.gz][ftp-ref]) +1. WXS reference archive (e.g. [SNV_INDEL_ref_GRCh37d5.tar.gz][ftp-ref]) +1. VAGrENT (annotation) reference archive (e.g. [VAGrENT_ref_GRCh37d5_ensembl_75.tar.gz][ftp-ref]) + +Inputs 1&2 are expected to have been mapped using [dockstore-cgpmap][dockstore-cgpmap] -Inputs 1&2 are expected to have been mapped using [dockstore-cgpmap](https://dockstore.org/containers/quay.io/wtsicgp/dockstore-cgpmap). +The data linked in the 'examples' area is a tiny section of chr21 from the cell line COLO-829. This +will generate a small number of INDEL and SNV variants. All the SNV variants do not pass filtering +(several only fail as not in a gene footprint, 'F009'). -The data linked in the 'examples' area is a tiny section of chr21 from the cell line COLO-829. This will generate a small number of INDEL and SNV variants. -All the SNV variants do not pass filtering (several only fail as not in a gene footprint, 'F009'). +Please check the Wiki then raise an issue if you require additional information on how to generate +your own reference files. Much of this information is available on the individual algorithm pages. -Please check the Wiki then raise an issue if you require additional information on how to generate your own reference files. Much of this information is available on the individual algorithm pages. +* [cgpCaVEManWrapper][caveman-wiki] +* [cgpPindel][cgppindel-wiki] +* [VAGrENT][vagrent-wiki] ## Usable Cores When running outside of a docker container you can set the number of CPUs via: * `export CPU=N` -* `-cores|-c` option of `ds-cgpmap.pl` +* `-cores|-c` option of `ds-cgpwxs.pl` If not set detects available cores on system. @@ -43,8 +54,8 @@ This project is maintained using HubFlow. 1. Push changes 1. Check state on Travis 1. Generate the release (add notes to GitHub) -1. Confirm that image has been built on [quay.io](https://quay.io/repository/wtsicgp/dockstore-cgpwxs?tab=builds) -1. Update the [dockstore](https://dockstore.org/containers/quay.io/wtsicgp/dockstore-cgpwxs) entry, see [their docs](https://dockstore.org/docs/getting-started-with-dockstore). +1. Confirm that image has been built on [quay.io][quay-builds] +1. Update the [dockstore][dockstore-cgpwgs] entry, see [their docs][dockstore-get-started]. ## LICENCE @@ -78,3 +89,28 @@ statement that reads ‘Copyright (c) 2005-2012’ should be interpreted as bein identical to a statement that reads ‘Copyright (c) 2005, 2006, 2007, 2008, 2009, 2010, 2011, 2012’." ``` + + +[ftp-ref]: ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human +[cgppindel-wiki]: https://github.com/cancerit/cgpPindel/wiki +[caveman-wiki]: https://github.com/cancerit/cgpCaVEManWrapper/wiki +[vagrent-wiki]: https://github.com/cancerit/VAGrENT/wiki + + +[travis-base]: https://travis-ci.org/cancerit/dockstore-cgpwxs +[travis-master]: https://travis-ci.org/cancerit/dockstore-cgpwxs.svg?branch=master +[travis-develop]: https://travis-ci.org/cancerit/dockstore-cgpwxs.svg?branch=develop + + +[gitter-svg]: https://badges.gitter.im/dockstore-cgp/Lobby.svg +[gitter-badge]: https://gitter.im/dockstore-cgp/Lobby?utm_source=badge&utm_medium=badge&utm_campaign=pr-badge&utm_content=badge + + +[quay-status]: https://quay.io/repository/wtsicgp/dockstore-cgpwxs/status +[quay-repo]: https://quay.io/repository/wtsicgp/dockstore-cgpwxs +[quay-builds]: https://quay.io/repository/wtsicgp/dockstore-cgpwxs?tab=builds + + +[dockstore-cgpwxs]: https://dockstore.org/containers/quay.io/wtsicgp/dockstore-cgpwxs +[dockstore-cgpmap]: https://dockstore.org/containers/quay.io/wtsicgp/dockstore-cgpmap +[dockstore-get-started]: https://dockstore.org/docs/getting-started-with-dockstore diff --git a/build/apt-build.sh b/build/apt-build.sh deleted file mode 100644 index 46cb5df..0000000 --- a/build/apt-build.sh +++ /dev/null @@ -1,5 +0,0 @@ -#!/bin/bash - -set -eux - -apt-get install -qy --no-install-recommends python diff --git a/cwls/cgpwxs.cwl b/cwls/cgpwxs.cwl index ae1f3af..67586f8 100644 --- a/cwls/cgpwxs.cwl +++ b/cwls/cgpwxs.cwl @@ -12,6 +12,9 @@ doc: | ![build_status](https://quay.io/repository/wtsicgp/dockstore-cgpwxs/status) A Docker container for the CGP WXS analysis flow. See the [dockstore-cgpwxs](https://github.com/cancerit/dockstore-cgpwxs) website for more information. + Please read the relevant [changes](https://github.com/cancerit/dockstore-cgpwxs/blob/master/CHANGES.md) + when upgrading. + Parameters for a CWL definition are generally described in a json file, but parameters can be provided on the command line. To see the parameters descriptions please run: cwltool --tool-help path_to.cwl diff --git a/scripts/analysisWXS.sh b/scripts/analysisWXS.sh index a994be9..88bd568 100755 --- a/scripts/analysisWXS.sh +++ b/scripts/analysisWXS.sh @@ -49,9 +49,6 @@ set -e echo -e "\nStart workflow: `date`\n" -declare -a PRE_EXEC -declare -a POST_EXEC - if [[ $# -eq 1 ]] ; then PARAM_FILE=$1 elif [ -z ${PARAM_FILE+x} ] ; then @@ -131,11 +128,13 @@ else ln -fs $BAM_WT.bas $BAM_WT_TMP.bas fi -## prime the cache -USER_CACHE=$OUTPUT_DIR/ref_cache -export REF_CACHE=$USER_CACHE/%2s/%2s/%s -export REF_PATH=$REF_CACHE:http://www.ebi.ac.uk/ena/cram/md5/%s -do_parallel[cache_POP]="seq_cache_populate.pl -root $USER_CACHE $REF_BASE/genome.fa" +if [ "$ALN_EXTN" == "cram" ]; then + ## prime the cache + USER_CACHE=$OUTPUT_DIR/ref_cache + export REF_CACHE=$USER_CACHE/%2s/%2s/%s + export REF_PATH=$REF_CACHE:http://www.ebi.ac.uk/ena/cram/md5/%s + do_parallel[cache_POP]="seq_cache_populate.pl -root $USER_CACHE $REF_BASE/genome.fa" +fi echo "Starting Parallel block 1: `date`" run_parallel $CPU do_parallel diff --git a/scripts/ds-cgpwxs.pl b/scripts/ds-cgpwxs.pl index 0993c38..749c062 100644 --- a/scripts/ds-cgpwxs.pl +++ b/scripts/ds-cgpwxs.pl @@ -67,12 +67,13 @@ # make the param file make_path($opts{'o'}) unless(-e $opts{'o'}); -my $run_file = $ENV{HOME}.'/run.params'; +my $run_file = $opts{'o'}.'/run.params'; open my $FH,'>',$run_file or die "Failed to write to $run_file: $!"; # Force explicit checking of file flush print $FH "export PCAP_THREADED_NO_SCRIPT=1\n"; print $FH "export PCAP_THREADED_FORCE_SYNC=1\n"; print $FH "export PCAP_THREADED_LOADBACKOFF=1\n"; +print $FH "export PCAP_THREADED_REM_LOGS=1\n"; # hard-coded printf $FH "PROTOCOL=WXS\n"; # required options From b608aeee7c467248eb5234e9eaafef7289e5c589 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Sun, 18 Feb 2018 17:10:50 +0000 Subject: [PATCH 30/54] Handle cases not using cram for cleanup --- scripts/analysisWXS.sh | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/scripts/analysisWXS.sh b/scripts/analysisWXS.sh index 88bd568..5bbacb8 100755 --- a/scripts/analysisWXS.sh +++ b/scripts/analysisWXS.sh @@ -225,7 +225,9 @@ if [ ! -z ${CLEAN_REF+x} ]; then fi # cleanup ref cache -rm -rf $USER_CACHE +if [ "$ALN_EXTN" == "cram" ]; then + rm -rf $USER_CACHE +fi echo 'Package results' # timings first From ca9423f920cb2de567574ec60781fd8fdac0d73e Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 27 Feb 2018 21:10:49 +0000 Subject: [PATCH 31/54] Resolves #11, SM could be end of line so cleanup line ending --- scripts/analysisWXS.sh | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/scripts/analysisWXS.sh b/scripts/analysisWXS.sh index 5bbacb8..f1e7ec1 100755 --- a/scripts/analysisWXS.sh +++ b/scripts/analysisWXS.sh @@ -81,8 +81,8 @@ set -u mkdir -p $OUTPUT_DIR ## get sample names from BAM headers -NAME_MT=`samtools view -H $BAM_MT | perl -ne 'if($_ =~ m/^\@RG/) {($sm) = $_ =~m/\tSM:([^\t]+)/; print "$sm\n";}' | uniq` -NAME_WT=`samtools view -H $BAM_WT | perl -ne 'if($_ =~ m/^\@RG/) {($sm) = $_ =~m/\tSM:([^\t]+)/; print "$sm\n";}' | uniq` +NAME_MT=`samtools view -H $BAM_MT | perl -ne 'chomp; if($_ =~ m/^\@RG/) {($sm) = $_ =~m/\tSM:([^\t]+)/; print "$sm\n";}' | uniq` +NAME_WT=`samtools view -H $BAM_WT | perl -ne 'chomp; if($_ =~ m/^\@RG/) {($sm) = $_ =~m/\tSM:([^\t]+)/; print "$sm\n";}' | uniq` echo -e "\tNAME_MT : $NAME_MT" echo -e "\tNAME_WT : $NAME_WT" From 769778674781c5e796b0cec79901004d3e434a4f Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 27 Feb 2018 21:12:07 +0000 Subject: [PATCH 32/54] Removed --- Dockstore.cwl | 1 - 1 file changed, 1 deletion(-) delete mode 120000 Dockstore.cwl diff --git a/Dockstore.cwl b/Dockstore.cwl deleted file mode 120000 index 9976871..0000000 --- a/Dockstore.cwl +++ /dev/null @@ -1 +0,0 @@ -cwls/cgpwxs.cwl \ No newline at end of file From 041965507f9737f8241565ea205f7a8f77795e0e Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 27 Feb 2018 21:12:42 +0000 Subject: [PATCH 33/54] Remove dct as replaced by schema approach --- cwls/cgpwxs.cwl | 7 +------ 1 file changed, 1 insertion(+), 6 deletions(-) diff --git a/cwls/cgpwxs.cwl b/cwls/cgpwxs.cwl index 67586f8..21bbbae 100644 --- a/cwls/cgpwxs.cwl +++ b/cwls/cgpwxs.cwl @@ -153,10 +153,5 @@ s:license: https://spdx.org/licenses/AGPL-3.0-only s:author: - class: s:Person s:identifier: https://orcid.org/0000-0002-5634-1539 - s:email: mailto:keiranmraine@gmail.com + s:email: mailto:cgphelp@sanger.ac.uk s:name: Keiran Raine - -dct:creator: - "@id": "keiranmraine@gmail.com" - foaf:name: Keiran Raine - foaf:mbox: "keiranmraine@gmail.com" From 1e5636f43bcbd454f00f92e3f5aa9be1e65ae947 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 27 Feb 2018 21:13:17 +0000 Subject: [PATCH 34/54] Add checks for tools that should be available --- .travis.yml | 22 +++++++++++++++++++++- 1 file changed, 21 insertions(+), 1 deletion(-) diff --git a/.travis.yml b/.travis.yml index 7249826..7f87a20 100644 --- a/.travis.yml +++ b/.travis.yml @@ -8,7 +8,27 @@ services: - docker install: - - docker build -t dockstore-cgpwxs . + - virtualenv -p python3 venv + - source venv/bin/activate + - pip install html5lib cwltool script: + - set -e + - cwltool --verify cwls/cgpwxs.cwl + - docker build -t dockstore-cgpwxs . - docker images | grep -c dockstore-cgpwxs + - docker run -ti --rm dockstore-cgpwxs ds-cgpmap.pl -h + - docker run -ti --rm dockstore-cgpwxs bwa_mem.pl -version + - docker run -ti --rm dockstore-cgpwxs bammarkduplicates2 --version + - docker run -ti --rm dockstore-cgpmap samtools --version + - docker run -ti --rm dockstore-cgpwxs bash -c 'bwa 2>&1 | grep Version' + - docker run -ti --rm dockstore-cgpwxs ds-cgpwxs.pl -h + - docker run -ti --rm dockstore-cgpwxs cgpVCFSplit.pl -version + - docker run -ti --rm dockstore-cgpwxs vcftools --version + - docker run -ti --rm dockstore-cgpwxs bedtools --version + - docker run -ti --rm dockstore-cgpwxs bash -c 'pindel 2>&1 | grep Welcome' + - docker run -ti --rm dockstore-cgpwxs pindel.pl -version + - docker run -ti --rm dockstore-cgpwxs caveman --version + - docker run -ti --rm dockstore-cgpwxs caveman.pl -version + - docker run -ti --rm dockstore-cgpwxs cgpFlagCaVEMan.pl -version + - docker run -ti --rm dockstore-cgpwxs AnnotateVcf.pl -version From 101f09534eeb846a7fe93475bdae4eba725a9016 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Fri, 2 Mar 2018 09:18:42 +0000 Subject: [PATCH 35/54] Cleaning up variables and duplication of mkdir --- .travis.yml | 8 +++++++- Dockerfile | 2 +- scripts/analysisWXS.sh | 17 ++++++++--------- scripts/ds-cgpwxs.pl | 2 +- 4 files changed, 17 insertions(+), 12 deletions(-) diff --git a/.travis.yml b/.travis.yml index 7f87a20..cc9bf69 100644 --- a/.travis.yml +++ b/.travis.yml @@ -14,14 +14,20 @@ install: script: - set -e - - cwltool --verify cwls/cgpwxs.cwl + - echo 'Validate CWL file(s)' + - cwltool --validate cwls/cgpwxs.cwl + - echo 'Build and check docker image' - docker build -t dockstore-cgpwxs . - docker images | grep -c dockstore-cgpwxs + - echo 'Verify program from each inherited package is found (dockstore-cgpbigwig)' + - docker run -ti --rm dockstore-cgpmap bwjoin --version + - echo 'Verify program from each inherited package is found (dockstore-cgpmap)' - docker run -ti --rm dockstore-cgpwxs ds-cgpmap.pl -h - docker run -ti --rm dockstore-cgpwxs bwa_mem.pl -version - docker run -ti --rm dockstore-cgpwxs bammarkduplicates2 --version - docker run -ti --rm dockstore-cgpmap samtools --version - docker run -ti --rm dockstore-cgpwxs bash -c 'bwa 2>&1 | grep Version' + - echo 'Verify a program from each new package is found (dockstore-cgpwxs)' - docker run -ti --rm dockstore-cgpwxs ds-cgpwxs.pl -h - docker run -ti --rm dockstore-cgpwxs cgpVCFSplit.pl -version - docker run -ti --rm dockstore-cgpwxs vcftools --version diff --git a/Dockerfile b/Dockerfile index f56ea43..704fee2 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM quay.io/wtsicgp/dockstore-cgpmap:3.0.0-rc8 as builder +FROM quay.io/wtsicgp/dockstore-cgpmap:3.0.0 as builder USER root diff --git a/scripts/analysisWXS.sh b/scripts/analysisWXS.sh index f1e7ec1..a55d24a 100755 --- a/scripts/analysisWXS.sh +++ b/scripts/analysisWXS.sh @@ -63,10 +63,6 @@ fi source $PARAM_FILE env -TMP=$OUTPUT_DIR/tmp -mkdir -p $TMP -mkdir -p $OUTPUT_DIR/timings - if [ -z ${CPU+x} ]; then CPU=`grep -c ^processor /proc/cpuinfo` fi @@ -78,7 +74,10 @@ echo -e "\tBAM_MT : $BAM_MT" echo -e "\tBAM_WT : $BAM_WT" set -u -mkdir -p $OUTPUT_DIR + +TMP=$OUTPUT_DIR/tmp +mkdir -p $TMP +mkdir -p $OUTPUT_DIR/timings ## get sample names from BAM headers NAME_MT=`samtools view -H $BAM_MT | perl -ne 'chomp; if($_ =~ m/^\@RG/) {($sm) = $_ =~m/\tSM:([^\t]+)/; print "$sm\n";}' | uniq` @@ -220,7 +219,7 @@ run_parallel $CPU do_parallel rm -rf $OUTPUT_DIR/${NAME_MT}_vs_${NAME_WT}/*/logs # cleanup reference area, see ds-cgpwxs.pl -if [ ! -z ${CLEAN_REF+x} ]; then +if [ $CLEAN_REF -gt 0 ]; then rm -rf $REF_BASE fi @@ -231,8 +230,8 @@ fi echo 'Package results' # timings first -tar -C $OUTPUT_DIR -zcf ${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.timings.tar.gz timings -tar -C $OUTPUT_DIR -zcf ${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.result.tar.gz ${NAME_MT}_vs_${NAME_WT} -cp $PARAM_FILE ${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.run.params +tar -C $OUTPUT_DIR -zcf $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.timings.tar.gz timings +tar -C $OUTPUT_DIR -zcf $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.result.tar.gz ${NAME_MT}_vs_${NAME_WT} +cp $PARAM_FILE $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.run.params echo -e "\nWorkflow end: `date`" diff --git a/scripts/ds-cgpwxs.pl b/scripts/ds-cgpwxs.pl index 749c062..014b18c 100644 --- a/scripts/ds-cgpwxs.pl +++ b/scripts/ds-cgpwxs.pl @@ -88,7 +88,7 @@ printf $FH "ASSEMBLY='%s'\n", $opts{'as'}; printf $FH "SNVFLAG='%s'\n", $ini; printf $FH "CPU=%d\n", $opts{'c'} if(defined $opts{'c'}); -printf $FH "CLEAN_REF=1\n" if($ref_unpack); +printf $FH "CLEAN_REF=%d\n", $ref_unpack; close $FH; exec('analysisWXS.sh', $run_file); # I will never return to the perl code From fbf2ff5636a57eb9e43039f08b72f64140f785e0 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Fri, 2 Mar 2018 09:36:01 +0000 Subject: [PATCH 36/54] Fix error in travis check --- .travis.yml | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/.travis.yml b/.travis.yml index cc9bf69..1877376 100644 --- a/.travis.yml +++ b/.travis.yml @@ -20,12 +20,12 @@ script: - docker build -t dockstore-cgpwxs . - docker images | grep -c dockstore-cgpwxs - echo 'Verify program from each inherited package is found (dockstore-cgpbigwig)' - - docker run -ti --rm dockstore-cgpmap bwjoin --version + - docker run -ti --rm dockstore-cgpwxs bwjoin --version - echo 'Verify program from each inherited package is found (dockstore-cgpmap)' - docker run -ti --rm dockstore-cgpwxs ds-cgpmap.pl -h - docker run -ti --rm dockstore-cgpwxs bwa_mem.pl -version - docker run -ti --rm dockstore-cgpwxs bammarkduplicates2 --version - - docker run -ti --rm dockstore-cgpmap samtools --version + - docker run -ti --rm dockstore-cgpwxs samtools --version - docker run -ti --rm dockstore-cgpwxs bash -c 'bwa 2>&1 | grep Version' - echo 'Verify a program from each new package is found (dockstore-cgpwxs)' - docker run -ti --rm dockstore-cgpwxs ds-cgpwxs.pl -h From fb05aae56528f416894fecefb99222e4738b95f5 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Fri, 2 Mar 2018 09:36:10 +0000 Subject: [PATCH 37/54] Expand docs --- README.md | 11 +++++++++++ 1 file changed, 11 insertions(+) diff --git a/README.md b/README.md index 8e89b58..cc1143e 100644 --- a/README.md +++ b/README.md @@ -12,6 +12,17 @@ associated annotation of VCF files. This has been packaged specifically for use | --------------------------------------------- | ----------------------------------------------- | | [![Master Badge][travis-master]][travis-base] | [![Develop Badge][travis-develop]][travis-base] | + + +- [Supported input formats](#supported-input-formats) +- [Usable Cores](#usable-cores) +- [Release process](#release-process) +- [LICENCE](#licence) + + + +## Supported input formats + Required input files are Note: cram and csi/crai will not work via Dockstore/CWL yet but direct exec of `scripts/ds-cgpwxs.pl` From 79099b2b5203d722a2dcab1b3d036a43487ac3ad Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Fri, 2 Mar 2018 11:08:31 +0000 Subject: [PATCH 38/54] Don't need interactive --- .travis.yml | 32 ++++++++++++++++---------------- 1 file changed, 16 insertions(+), 16 deletions(-) diff --git a/.travis.yml b/.travis.yml index 1877376..6d09cdb 100644 --- a/.travis.yml +++ b/.travis.yml @@ -20,21 +20,21 @@ script: - docker build -t dockstore-cgpwxs . - docker images | grep -c dockstore-cgpwxs - echo 'Verify program from each inherited package is found (dockstore-cgpbigwig)' - - docker run -ti --rm dockstore-cgpwxs bwjoin --version + - docker run -t --rm dockstore-cgpwxs bwjoin --version - echo 'Verify program from each inherited package is found (dockstore-cgpmap)' - - docker run -ti --rm dockstore-cgpwxs ds-cgpmap.pl -h - - docker run -ti --rm dockstore-cgpwxs bwa_mem.pl -version - - docker run -ti --rm dockstore-cgpwxs bammarkduplicates2 --version - - docker run -ti --rm dockstore-cgpwxs samtools --version - - docker run -ti --rm dockstore-cgpwxs bash -c 'bwa 2>&1 | grep Version' + - docker run -t --rm dockstore-cgpwxs ds-cgpmap.pl -h + - docker run -t --rm dockstore-cgpwxs bwa_mem.pl -version + - docker run -t --rm dockstore-cgpwxs bammarkduplicates2 --version + - docker run -t --rm dockstore-cgpwxs samtools --version + - docker run -t --rm dockstore-cgpwxs bash -c 'bwa 2>&1 | grep Version' - echo 'Verify a program from each new package is found (dockstore-cgpwxs)' - - docker run -ti --rm dockstore-cgpwxs ds-cgpwxs.pl -h - - docker run -ti --rm dockstore-cgpwxs cgpVCFSplit.pl -version - - docker run -ti --rm dockstore-cgpwxs vcftools --version - - docker run -ti --rm dockstore-cgpwxs bedtools --version - - docker run -ti --rm dockstore-cgpwxs bash -c 'pindel 2>&1 | grep Welcome' - - docker run -ti --rm dockstore-cgpwxs pindel.pl -version - - docker run -ti --rm dockstore-cgpwxs caveman --version - - docker run -ti --rm dockstore-cgpwxs caveman.pl -version - - docker run -ti --rm dockstore-cgpwxs cgpFlagCaVEMan.pl -version - - docker run -ti --rm dockstore-cgpwxs AnnotateVcf.pl -version + - docker run -t --rm dockstore-cgpwxs ds-cgpwxs.pl -h + - docker run -t --rm dockstore-cgpwxs cgpVCFSplit.pl -version + - docker run -t --rm dockstore-cgpwxs vcftools --version + - docker run -t --rm dockstore-cgpwxs bedtools --version + - docker run -t --rm dockstore-cgpwxs bash -c 'pindel 2>&1 | grep Welcome' + - docker run -t --rm dockstore-cgpwxs pindel.pl -version + - docker run -t --rm dockstore-cgpwxs caveman --version + - docker run -t --rm dockstore-cgpwxs caveman.pl -version + - docker run -t --rm dockstore-cgpwxs cgpFlagCaVEMan.pl -version + - docker run -t --rm dockstore-cgpwxs AnnotateVcf.pl -version From c75bcbcbf745f51c03f7479ccb214b27fbd2a9c9 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Fri, 2 Mar 2018 11:08:53 +0000 Subject: [PATCH 39/54] Add forked Bio::DB::HTS --- build/opt-build.sh | 46 ++++++++++++++++++++++++++++++++++++++++++++++ 1 file changed, 46 insertions(+) diff --git a/build/opt-build.sh b/build/opt-build.sh index 560ed34..8f57632 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -8,6 +8,13 @@ fi set -u +#### THIS SHOULD BE MOVED TO dockstore-cgpmap on next release +# +VER_HTSLIB="1.7" +CANCERIT_BIODBHTS="https://github.com/cancerit/Bio-DB-HTS/archive/v2.10-rc1.tar.gz" +# +#### THIS SHOULD BE MOVED TO dockstore-cgpmap on next release + # cgpVcf VER_CGPVCF="v2.2.1" VER_VCFTOOLS="0.1.15" @@ -70,6 +77,45 @@ export MANPATH=`echo $INST_PATH/man:$INST_PATH/share/man:$MANPATH | perl -pe 's/ export PERL5LIB=`echo $INST_PATH/lib/perl5:$PERL5LIB | perl -pe 's/:\$//;'` set -u + +#### THIS SHOULD BE MOVED TO dockstore-cgpmap on next release +# +# HTSLIB (tar.bz2) +if [ ! -e $SETUP_DIR/htslib.success ]; then + rm -rf htslib + mkdir -p htslib + curl -sSL --retry 10 https://github.com/samtools/htslib/releases/download/${VER_HTSLIB}/htslib-${VER_HTSLIB}.tar.bz2 > distro.tar.bz2 + tar --strip-components 1 -C htslib -jxf distro.tar.bz2 + cd htslib + ./configure --enable-plugins --enable-libcurl --prefix=$INST_PATH + make clean + make -j$CPU + make install + cd $SETUP_DIR + rm -rf distro.* + touch $SETUP_DIR/htslib.success +fi +# Bio::DB::HTS (tar.gz) +if [ ! -e $SETUP_DIR/Bio-DB-HTS.success ]; then + ## add perl deps + cpanm --no-wget --no-interactive --notest --mirror http://cpan.metacpan.org -l $INST_PATH Module::Build + cpanm --no-wget --no-interactive --notest --mirror http://cpan.metacpan.org -l $INST_PATH Bio::Root::Version + + curl -sSL --retry 10 $CANCERIT_BIODBHTS > distro.tar.gz + rm -rf distro/* + tar --strip-components 1 -C distro -zxf distro.tar.gz + cd distro + perl Build.PL --install_base=$INST_PATH --htslib=$INST_PATH + ./Build + ./Build test + ./Build install + cd $SETUP_DIR + rm -rf distro.* distro/* + touch $SETUP_DIR/Bio-DB-HTS.success +fi +# +#### THIS SHOULD BE MOVED TO dockstore-cgpmap on next release + ## vcftools if [ ! -e $SETUP_DIR/vcftools.success ]; then curl -sSL --retry 10 https://github.com/vcftools/vcftools/releases/download/v${VER_VCFTOOLS}/vcftools-${VER_VCFTOOLS}.tar.gz > distro.tar.gz From 5b09c329223f39e5c91ff29406d6648efb99dbff Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Fri, 2 Mar 2018 11:23:10 +0000 Subject: [PATCH 40/54] Remove exit on error as travis doesn't highlight the error case then, comments are allowed so drop inline echo --- .travis.yml | 13 +++++++------ 1 file changed, 7 insertions(+), 6 deletions(-) diff --git a/.travis.yml b/.travis.yml index 6d09cdb..9886fa8 100644 --- a/.travis.yml +++ b/.travis.yml @@ -4,6 +4,8 @@ notifications: sudo: false +language: ruby + services: - docker @@ -13,21 +15,20 @@ install: - pip install html5lib cwltool script: - - set -e - - echo 'Validate CWL file(s)' + # Validate CWL file(s) - cwltool --validate cwls/cgpwxs.cwl - - echo 'Build and check docker image' + # Build and check docker image - docker build -t dockstore-cgpwxs . - docker images | grep -c dockstore-cgpwxs - - echo 'Verify program from each inherited package is found (dockstore-cgpbigwig)' + # Verify program from each inherited package is found (dockstore-cgpbigwig) - docker run -t --rm dockstore-cgpwxs bwjoin --version - - echo 'Verify program from each inherited package is found (dockstore-cgpmap)' + # Verify program from each inherited package is found (dockstore-cgpmap) - docker run -t --rm dockstore-cgpwxs ds-cgpmap.pl -h - docker run -t --rm dockstore-cgpwxs bwa_mem.pl -version - docker run -t --rm dockstore-cgpwxs bammarkduplicates2 --version - docker run -t --rm dockstore-cgpwxs samtools --version - docker run -t --rm dockstore-cgpwxs bash -c 'bwa 2>&1 | grep Version' - - echo 'Verify a program from each new package is found (dockstore-cgpwxs)' + # Verify a program from each new package is found (dockstore-cgpwxs) - docker run -t --rm dockstore-cgpwxs ds-cgpwxs.pl -h - docker run -t --rm dockstore-cgpwxs cgpVCFSplit.pl -version - docker run -t --rm dockstore-cgpwxs vcftools --version From 00cdff4eb3b15d58c7e611268df2c3610df9b8dd Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Fri, 2 Mar 2018 11:23:41 +0000 Subject: [PATCH 41/54] Temporary libs to allow building of forked Bio::DB::HTS --- Dockerfile | 9 +++++++++ 1 file changed, 9 insertions(+) diff --git a/Dockerfile b/Dockerfile index 704fee2..3c8bcc2 100644 --- a/Dockerfile +++ b/Dockerfile @@ -14,6 +14,15 @@ RUN apt-get install -yq --no-install-recommends\ # python only for building bedtools, not needed to use +### TO REMOVE once dockstore-cgpmap has fixed Bio::DB::HTS for CSI index +# +RUN apt-get install -yq --no-install-recommends\ + libbz2-dev\ + liblzma-dev\ + libcurl4-gnutls-dev +# +### + RUN locale-gen en_US.UTF-8 RUN update-locale LANG=en_US.UTF-8 From 5b634640210ab2b4c7e540399f5faa53fe5a37c5 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Fri, 2 Mar 2018 15:50:48 +0000 Subject: [PATCH 42/54] Updated cgpPindel and dependent image now our fork of Bio::DB::HTS in use --- Dockerfile | 11 +---------- build/opt-build.sh | 48 +--------------------------------------------- 2 files changed, 2 insertions(+), 57 deletions(-) diff --git a/Dockerfile b/Dockerfile index 3c8bcc2..ae411bd 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM quay.io/wtsicgp/dockstore-cgpmap:3.0.0 as builder +FROM quay.io/wtsicgp/dockstore-cgpmap:3.0.1 as builder USER root @@ -14,15 +14,6 @@ RUN apt-get install -yq --no-install-recommends\ # python only for building bedtools, not needed to use -### TO REMOVE once dockstore-cgpmap has fixed Bio::DB::HTS for CSI index -# -RUN apt-get install -yq --no-install-recommends\ - libbz2-dev\ - liblzma-dev\ - libcurl4-gnutls-dev -# -### - RUN locale-gen en_US.UTF-8 RUN update-locale LANG=en_US.UTF-8 diff --git a/build/opt-build.sh b/build/opt-build.sh index 8f57632..eb7e8c6 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -8,19 +8,12 @@ fi set -u -#### THIS SHOULD BE MOVED TO dockstore-cgpmap on next release -# -VER_HTSLIB="1.7" -CANCERIT_BIODBHTS="https://github.com/cancerit/Bio-DB-HTS/archive/v2.10-rc1.tar.gz" -# -#### THIS SHOULD BE MOVED TO dockstore-cgpmap on next release - # cgpVcf VER_CGPVCF="v2.2.1" VER_VCFTOOLS="0.1.15" # cgpPindel -VER_CGPPINDEL="feature/readsVsFragments" +VER_CGPPINDEL="v3.0.0" # cgpCaVEManPostProcessing VER_CGPCAVEPOSTPROC="feature/overlapping_reads" @@ -77,45 +70,6 @@ export MANPATH=`echo $INST_PATH/man:$INST_PATH/share/man:$MANPATH | perl -pe 's/ export PERL5LIB=`echo $INST_PATH/lib/perl5:$PERL5LIB | perl -pe 's/:\$//;'` set -u - -#### THIS SHOULD BE MOVED TO dockstore-cgpmap on next release -# -# HTSLIB (tar.bz2) -if [ ! -e $SETUP_DIR/htslib.success ]; then - rm -rf htslib - mkdir -p htslib - curl -sSL --retry 10 https://github.com/samtools/htslib/releases/download/${VER_HTSLIB}/htslib-${VER_HTSLIB}.tar.bz2 > distro.tar.bz2 - tar --strip-components 1 -C htslib -jxf distro.tar.bz2 - cd htslib - ./configure --enable-plugins --enable-libcurl --prefix=$INST_PATH - make clean - make -j$CPU - make install - cd $SETUP_DIR - rm -rf distro.* - touch $SETUP_DIR/htslib.success -fi -# Bio::DB::HTS (tar.gz) -if [ ! -e $SETUP_DIR/Bio-DB-HTS.success ]; then - ## add perl deps - cpanm --no-wget --no-interactive --notest --mirror http://cpan.metacpan.org -l $INST_PATH Module::Build - cpanm --no-wget --no-interactive --notest --mirror http://cpan.metacpan.org -l $INST_PATH Bio::Root::Version - - curl -sSL --retry 10 $CANCERIT_BIODBHTS > distro.tar.gz - rm -rf distro/* - tar --strip-components 1 -C distro -zxf distro.tar.gz - cd distro - perl Build.PL --install_base=$INST_PATH --htslib=$INST_PATH - ./Build - ./Build test - ./Build install - cd $SETUP_DIR - rm -rf distro.* distro/* - touch $SETUP_DIR/Bio-DB-HTS.success -fi -# -#### THIS SHOULD BE MOVED TO dockstore-cgpmap on next release - ## vcftools if [ ! -e $SETUP_DIR/vcftools.success ]; then curl -sSL --retry 10 https://github.com/vcftools/vcftools/releases/download/v${VER_VCFTOOLS}/vcftools-${VER_VCFTOOLS}.tar.gz > distro.tar.gz From 8dee8e65ac7c4ef80e6fad2bf5b614bb20602a94 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 7 Mar 2018 09:32:09 +0000 Subject: [PATCH 43/54] Version and cleaning of email/license details --- CHANGES.md | 2 +- Dockerfile | 4 +-- README.md | 69 ++++++++++++++++++++++++++++++++++++++++++-- build/opt-build.sh | 8 ++--- scripts/ds-cgpwxs.pl | 8 ++--- 5 files changed, 77 insertions(+), 14 deletions(-) diff --git a/CHANGES.md b/CHANGES.md index b69aa03..376b929 100644 --- a/CHANGES.md +++ b/CHANGES.md @@ -7,7 +7,7 @@ * You will need to update the reference pack to include the new flagging rules, see example `json` files * Using build stages to shrink images. -* remove legacy PRE/POST-EXEC from cgpbox days +* remove legacy PRE/POST-EXEC from cgpbox days, use dockstore if you want file provisioning. ## 2.1.0 diff --git a/Dockerfile b/Dockerfile index ae411bd..fe41431 100644 --- a/Dockerfile +++ b/Dockerfile @@ -29,9 +29,9 @@ RUN bash build/opt-build.sh $OPT FROM ubuntu:16.04 -MAINTAINER keiranmraine@gmail.com +MAINTAINER cgphelp@sanger.ac.uk -LABEL vendor="Cancer Genome Project, Wellcome Trust Sanger Institute" +LABEL vendor="CASM/Cancer IT, Wellcome Sanger Institute" LABEL uk.ac.sanger.cgp.description="CGP WXS pipeline for dockstore.org" LABEL uk.ac.sanger.cgp.version="3.0.0-rc1" diff --git a/README.md b/README.md index cc1143e..9459895 100644 --- a/README.md +++ b/README.md @@ -16,6 +16,10 @@ associated annotation of VCF files. This has been packaged specifically for use - [Supported input formats](#supported-input-formats) - [Usable Cores](#usable-cores) +- [Other uses](#other-uses) + - [Native docker](#native-docker) + - [Singularity](#singularity) + - [Test data](#test-data) - [Release process](#release-process) - [LICENCE](#licence) @@ -56,6 +60,66 @@ When running outside of a docker container you can set the number of CPUs via: If not set detects available cores on system. +## Other uses + +xxx + +### Native docker + +All of the tools installed as part of [dockstore-cgpmap][dockstore-cgpmap] and the above packages +are available for direct use. + +``` +export CGPWXS_VER=X.X.X +docker pull quay.io/wtsicgp/dockstore-cgpwxs:$CGPWXS_VER +docker --rm -ti [--volume ...] quay.io/wtsicgp/dockstore-cgpwxs:$CGPWXS_VER bash +``` + +### Singularity + +The resulting docker container has been tested with Singularity. The command to exec is: + +``` +ds-cgpwxs.pl -h +``` + +Expected use would be along the lines of: + +``` +export CGPWXS_VER=X.X.X +singularity pull docker://quay.io/wtsicgp/dockstore-cgpwxs:$CGPWXS_VER + +singularity exec\ + --workdir /.../workspace \ + --home /.../workspace:/home \ + --bind /.../ref/human:/var/spool/ref:ro \ + --bind /.../example_data/cgpwxs:/var/spool/data:ro \ + dockstore-cgpwxs-${CGPWXS_VER}.simg \ + ds-cgpwxs.pl \ + -reference /var/spool/ref/core_ref_GRCh37d5.tar.gz \ + -annot /var/spool/ref/VAGrENT_ref_GRCh37d5_ensembl_75.tar.gz \ + -snv_indel /var/spool/ref/SNV_INDEL_ref_GRCh37d5-fragment.tar.gz \ + -tumour /var/spool/data/COLO-829_ex.cram \ + -tidx /var/spool/data/COLO-829_ex.cram.crai \ + -normal /var/spool/data/COLO-829-BL_ex.cram \ + -nidx /var/spool/data/COLO-829-BL_ex.cram.crai \ + -exclude NC_007605,hs37d5,GL% +``` + +For a system automatically attaching _all local mount points_ (not default singularity behaviour) +you need not specify any `exec` params (workdir, home, bind) but you should specify the `-outdir` +option for `ds-cgpwxs.pl` to prevent data being written to your home directory. + +By default results are written to the home directory of the container so ensure you bind +a large volume and set the `-home` variable. As indicated above the location can be overridden +via the options of `ds-cgpwxs.pl` + +### Test data + +The `examples/` contains test data that can be used to verify the tool. + +You can find expected outputs on the Sanger Institute FTP site (based on BAM+BAI inputs): [dockstore-cgpwxs-expected.tar.gz][cgpwxs-exp] + ## Release process This project is maintained using HubFlow. @@ -73,7 +137,7 @@ This project is maintained using HubFlow. ``` Copyright (c) 2016-2018 Genome Research Ltd. -Author: Cancer Genome Project +Author: CASM/Cancer IT This file is part of dockstore-cgpwxs. @@ -103,6 +167,7 @@ identical to a statement that reads ‘Copyright (c) 2005, 2006, 2007, 2008, [ftp-ref]: ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human +[ftp-exp]: ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/expected [cgppindel-wiki]: https://github.com/cancerit/cgpPindel/wiki [caveman-wiki]: https://github.com/cancerit/cgpCaVEManWrapper/wiki [vagrent-wiki]: https://github.com/cancerit/VAGrENT/wiki @@ -123,5 +188,5 @@ identical to a statement that reads ‘Copyright (c) 2005, 2006, 2007, 2008, [dockstore-cgpwxs]: https://dockstore.org/containers/quay.io/wtsicgp/dockstore-cgpwxs -[dockstore-cgpmap]: https://dockstore.org/containers/quay.io/wtsicgp/dockstore-cgpmap +[dockstore-cgpmap]: https://dockstore.org/containers/quay.io/wtsicgp/dockstore-cgpwxs [dockstore-get-started]: https://dockstore.org/docs/getting-started-with-dockstore diff --git a/build/opt-build.sh b/build/opt-build.sh index eb7e8c6..8a11b5b 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -16,13 +16,13 @@ VER_VCFTOOLS="0.1.15" VER_CGPPINDEL="v3.0.0" # cgpCaVEManPostProcessing -VER_CGPCAVEPOSTPROC="feature/overlapping_reads" +VER_CGPCAVEPOSTPROC="1.8.0" # if issues found downgrade to 2.23.0 but can't find any use of bedtools coverage VER_BEDTOOLS="2.27.1" # cgpCaVEManWrapper -VER_CGPCAVEWRAP="feature/overlapping_reads" -VER_CAVEMAN="1.13.0-rc1" +VER_CGPCAVEWRAP="1.13.0" +VER_CAVEMAN="1.13.0" # VAGrENT VER_VAGRENT="v3.2.3" @@ -32,7 +32,6 @@ if [ "$#" -lt "1" ] ; then exit 1 fi - # get path to this script SCRIPT_PATH=`dirname $0`; SCRIPT_PATH=`(cd $SCRIPT_PATH && pwd)` @@ -56,7 +55,6 @@ else fi echo "Max compilation CPUs set to $CPU" - SETUP_DIR=$INIT_DIR/install_tmp mkdir -p $SETUP_DIR/distro # don't delete the actual distro directory until the very end mkdir -p $INST_PATH/bin diff --git a/scripts/ds-cgpwxs.pl b/scripts/ds-cgpwxs.pl index 014b18c..8226e48 100644 --- a/scripts/ds-cgpwxs.pl +++ b/scripts/ds-cgpwxs.pl @@ -100,27 +100,27 @@ sub resolve_sp_as { my ($wt_species, $wt_assembly) = species_assembly_from_xam($options->{'n'}); if($mt_species ne $wt_species) { warn "WARN: Species mismatch between T/N [CR|B]AM headers\n"; - if(!defined $options->{'sp'}) { + if(!defined $options->{'sp'} || $options->{'sp'} eq q{}) { die "ERROR: Please define species to handle this mismatch\n"; } } elsif($mt_species ne q{}) { $options->{'sp'} = $mt_species; } - if(!defined $options->{'sp'}) { + if(!defined $options->{'sp'} || $options->{'sp'} eq q{}) { die "ERROR: Please define species, not found in [CR|B]AM headers.\n"; } if($mt_assembly ne $wt_assembly) { warn "WARN: Assembly mismatch between T/N [CR|B]AM headers\n"; - if(!defined $options->{'as'}) { + if(!defined $options->{'as'} || $options->{'as'} eq q{}) { die "ERROR: Please define assembly to handle this mismatch\n"; } } elsif($mt_assembly ne q{}) { $options->{'as'} = $mt_assembly; } - if(!defined $options->{'as'}) { + if(!defined $options->{'as'} || $options->{'as'} eq q{}) { die "ERROR: Please define assembly, not found in [CR|B]AM headers.\n"; } } From 1f86768bf8922e15ba73647e7228efcbbc97930c Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 7 Mar 2018 12:00:51 +0000 Subject: [PATCH 44/54] Bump VAGrENT version --- CHANGES.md | 1 + build/opt-build.sh | 2 +- 2 files changed, 2 insertions(+), 1 deletion(-) diff --git a/CHANGES.md b/CHANGES.md index 376b929..d2a7872 100644 --- a/CHANGES.md +++ b/CHANGES.md @@ -6,6 +6,7 @@ * CaVEMan and cgpPindel updated to use fragment based counting. * You will need to update the reference pack to include the new flagging rules, see example `json` files +* VAGrENT update to v3.3.0, only affects generation of annotation cache not use of existing ones. * Using build stages to shrink images. * remove legacy PRE/POST-EXEC from cgpbox days, use dockstore if you want file provisioning. diff --git a/build/opt-build.sh b/build/opt-build.sh index 8a11b5b..0f47205 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -25,7 +25,7 @@ VER_CGPCAVEWRAP="1.13.0" VER_CAVEMAN="1.13.0" # VAGrENT -VER_VAGRENT="v3.2.3" +VER_VAGRENT="v3.3.0" if [ "$#" -lt "1" ] ; then echo "Please provide an installation path such as /opt/ICGC" From 3304ae0ea66c88f278c0ded12b86afa708110b32 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 7 Mar 2018 20:34:56 +0000 Subject: [PATCH 45/54] Fix issue with bad param in caveman postprocessing and add quotes to species --- build/opt-build.sh | 2 +- scripts/analysisWXS.sh | 4 ++-- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/build/opt-build.sh b/build/opt-build.sh index 0f47205..c5a09b0 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -16,7 +16,7 @@ VER_VCFTOOLS="0.1.15" VER_CGPPINDEL="v3.0.0" # cgpCaVEManPostProcessing -VER_CGPCAVEPOSTPROC="1.8.0" +VER_CGPCAVEPOSTPROC="1.8.1" # if issues found downgrade to 2.23.0 but can't find any use of bedtools coverage VER_BEDTOOLS="2.27.1" diff --git a/scripts/analysisWXS.sh b/scripts/analysisWXS.sh index a55d24a..b06df3c 100755 --- a/scripts/analysisWXS.sh +++ b/scripts/analysisWXS.sh @@ -156,7 +156,7 @@ do_parallel[cgpPindel]="pindel.pl \ -g $REF_BASE/vagrent/codingexon_regions.indel.bed.gz \ -st $PROTOCOL \ -as $ASSEMBLY \ - -sp $SPECIES \ + -sp '$SPECIES' \ -e $CONTIG_EXCLUDE \ -b $REF_BASE/pindel/HiDepth.bed.gz \ -c $CPU \ @@ -174,7 +174,7 @@ do_parallel[CaVEMan]="caveman.pl \ -b $REF_BASE/caveman/flagging \ -ab $REF_BASE/vagrent \ -u $REF_BASE/caveman \ - -s $SPECIES \ + -s '$SPECIES' \ -sa $ASSEMBLY \ -t $CPU \ -st $PROTOCOL \ From 902389acab220584cf589c1e6b7d04fe5f6fd9e5 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Thu, 8 Mar 2018 14:38:16 +0000 Subject: [PATCH 46/54] Make example outputs have distinct names --- examples/cgpwxs/bam_bai.json | 6 +++--- examples/cgpwxs/bam_csi.json | 6 +++--- examples/cgpwxs/cram_crai.json | 6 +++--- 3 files changed, 9 insertions(+), 9 deletions(-) diff --git a/examples/cgpwxs/bam_bai.json b/examples/cgpwxs/bam_bai.json index f74507f..d771808 100644 --- a/examples/cgpwxs/bam_bai.json +++ b/examples/cgpwxs/bam_bai.json @@ -29,15 +29,15 @@ }, "exclude": "1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,22,X,Y,MT,NC_007605,hs37d5,GL%", "result_archive": { - "path": "/tmp/result_WXS.tar.gz", + "path": "/tmp/result_WXS_bam_bai.tar.gz", "class": "File" }, "timings": { - "path": "/tmp/timings_WXS.tar.gz", + "path": "/tmp/timings_WXS_bam_bai.tar.gz", "class": "File" }, "run_params": { - "path": "/tmp/params_WXS.params", + "path": "/tmp/params_WXS_bam_bai.params", "class": "File" } } diff --git a/examples/cgpwxs/bam_csi.json b/examples/cgpwxs/bam_csi.json index 647ae02..099e84e 100644 --- a/examples/cgpwxs/bam_csi.json +++ b/examples/cgpwxs/bam_csi.json @@ -29,15 +29,15 @@ }, "exclude": "1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,22,X,Y,MT,NC_007605,hs37d5,GL%", "result_archive": { - "path": "/tmp/result_WXS.tar.gz", + "path": "/tmp/result_WXS_bam_csi.tar.gz", "class": "File" }, "timings": { - "path": "/tmp/timings_WXS.tar.gz", + "path": "/tmp/timings_WXS_bam_csi.tar.gz", "class": "File" }, "run_params": { - "path": "/tmp/params_WXS.params", + "path": "/tmp/params_WXS_bam_csi.params", "class": "File" } } diff --git a/examples/cgpwxs/cram_crai.json b/examples/cgpwxs/cram_crai.json index 4b5a884..ec8f348 100644 --- a/examples/cgpwxs/cram_crai.json +++ b/examples/cgpwxs/cram_crai.json @@ -29,15 +29,15 @@ }, "exclude": "1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,22,X,Y,MT,NC_007605,hs37d5,GL%", "result_archive": { - "path": "/tmp/result_WXS.tar.gz", + "path": "/tmp/result_WXS_cram_crai.tar.gz", "class": "File" }, "timings": { - "path": "/tmp/timings_WXS.tar.gz", + "path": "/tmp/timings_WXS_cram_crai.tar.gz", "class": "File" }, "run_params": { - "path": "/tmp/params_WXS.params", + "path": "/tmp/params_WXS_cram_crai.params", "class": "File" } } From b27a768144695c4d35cad34dcdbdb9ed00b876af Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Thu, 8 Mar 2018 16:34:37 +0000 Subject: [PATCH 47/54] Document the use of the hooks --- README.md | 14 +++++++-- git-hooks/pre-commit | 67 ++++++++++++++++++++++++++++++++++++++++++++ 2 files changed, 79 insertions(+), 2 deletions(-) create mode 100755 git-hooks/pre-commit diff --git a/README.md b/README.md index 9459895..f23038a 100644 --- a/README.md +++ b/README.md @@ -62,8 +62,6 @@ If not set detects available cores on system. ## Other uses -xxx - ### Native docker All of the tools installed as part of [dockstore-cgpmap][dockstore-cgpmap] and the above packages @@ -120,6 +118,18 @@ The `examples/` contains test data that can be used to verify the tool. You can find expected outputs on the Sanger Institute FTP site (based on BAM+BAI inputs): [dockstore-cgpwxs-expected.tar.gz][cgpwxs-exp] +## Development environment + +This project uses git pre-commit hooks. Please enable them to prevent inappropriate large files +being included. Any pull request found not to have adhered to this will be rejected and the branch +will need to be manually cleaned to keep the repo size down. + +Activate the hooks with + +``` +git config core.hooksPath git-hooks +``` + ## Release process This project is maintained using HubFlow. diff --git a/git-hooks/pre-commit b/git-hooks/pre-commit new file mode 100755 index 0000000..840803b --- /dev/null +++ b/git-hooks/pre-commit @@ -0,0 +1,67 @@ +#!/bin/sh +# +# An example hook script to verify what is about to be committed. +# Called by "git commit" with no arguments. The hook should +# exit with non-zero status after issuing an appropriate message if +# it wants to stop the commit. +# +# To enable this hook, rename this file to "pre-commit". + +if git rev-parse --verify HEAD >/dev/null 2>&1 +then + against=HEAD +else + # Initial commit: diff against an empty tree object + against=4b825dc642cb6eb9a060e54bf8d69288fbee4904 +fi + +# If you want to allow non-ASCII filenames set this variable to true. +allownonascii=$(git config --bool hooks.allownonascii) + +# Redirect output to stderr. +exec 1>&2 + +# Cross platform projects tend to avoid non-ASCII filenames; prevent +# them from being added to the repository. We exploit the fact that the +# printable range starts at the space character and ends with tilde. +if [ "$allownonascii" != "true" ] && + # Note that the use of brackets around a tr range is ok here, (it's + # even required, for portability to Solaris 10's /usr/bin/tr), since + # the square bracket bytes happen to fall in the designated range. + test $(git diff --cached --name-only --diff-filter=A -z $against | + LC_ALL=C tr -d '[ -~]\0' | wc -c) != 0 +then + cat <<\EOF +Error: Attempt to add a non-ASCII file name. + +This can cause problems if you want to work with people on other platforms. + +To be portable it is advisable to rename the file. + +If you know what you are doing you can disable this check using: + + git config hooks.allownonascii true +EOF + exit 1 +fi + +## Now we want to warn that certain files should have size==0 +TO_MINIMISE=`find expected -type f \( -iname \*.bai -o -iname \*.crai -o -iname \*.tbi -o -iname \*.md5 \) -size +1c` + +if [ ! -z "$TO_MINIMISE" ]; then + >&2 cat << EOF + +ERROR: The following files should be truncated to size of 0 before commit: + +$TO_MINIMISE + + To do this quickly run: + + find expected -type f \( -iname \*.bai -o -iname \*.crai -o -iname \*.tbi -o -iname \*.md5 \) -size +1c -exec bash -c 'echo > {}' \; + +EOF + exit 1 +fi + +# If there are whitespace errors, print the offending file names and fail. +exec git diff-index --check --cached $against -- From 801c63d823dbcd8838a3d1b134602bd09c9924f2 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Thu, 8 Mar 2018 16:43:12 +0000 Subject: [PATCH 48/54] truncate caveman prob_arr too --- git-hooks/pre-commit | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/git-hooks/pre-commit b/git-hooks/pre-commit index 840803b..ceba299 100755 --- a/git-hooks/pre-commit +++ b/git-hooks/pre-commit @@ -46,7 +46,7 @@ EOF fi ## Now we want to warn that certain files should have size==0 -TO_MINIMISE=`find expected -type f \( -iname \*.bai -o -iname \*.crai -o -iname \*.tbi -o -iname \*.md5 \) -size +1c` +TO_MINIMISE=`find expected -type f \( -iname \*.bai -o -iname \*.crai -o -iname \*.tbi -o -iname \*.md5 -o -iname prob_arr \) -size +1c` if [ ! -z "$TO_MINIMISE" ]; then >&2 cat << EOF @@ -57,7 +57,7 @@ $TO_MINIMISE To do this quickly run: - find expected -type f \( -iname \*.bai -o -iname \*.crai -o -iname \*.tbi -o -iname \*.md5 \) -size +1c -exec bash -c 'echo > {}' \; + find expected -type f \( -iname \*.bai -o -iname \*.crai -o -iname \*.tbi -o -iname \*.md5 -o -iname prob_arr \) -size +1c -exec bash -c 'echo > {}' \; EOF exit 1 From 7f6b78e1e77de852db805946ef5182835938d665 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Thu, 8 Mar 2018 16:44:13 +0000 Subject: [PATCH 49/54] Add the first round of embedded expected data --- README.md | 4 +- examples/README.md | 11 ++ expected/README.md | 67 +++++++ .../COLO-829_vs_COLO-829-BL.annot.muts.vcf.gz | Bin 0 -> 3003 bytes ...O-829_vs_COLO-829-BL.annot.muts.vcf.gz.tbi | 1 + ...OLO-829_vs_COLO-829-BL.flagged.muts.vcf.gz | Bin 0 -> 2725 bytes ...829_vs_COLO-829-BL.flagged.muts.vcf.gz.tbi | 1 + .../COLO-829_vs_COLO-829-BL.muts.ids.vcf.gz | Bin 0 -> 1794 bytes ...OLO-829_vs_COLO-829-BL.muts.ids.vcf.gz.tbi | 1 + .../COLO-829_vs_COLO-829-BL.no_analysis.bed | 180 ++++++++++++++++++ .../COLO-829_vs_COLO-829-BL.snps.ids.vcf.gz | Bin 0 -> 5883 bytes ...OLO-829_vs_COLO-829-BL.snps.ids.vcf.gz.tbi | 1 + .../COLO-829_vs_COLO-829-BL/caveman/alg_bean | 4 + .../caveman/caveman.cfg.ini | 14 ++ .../COLO-829_vs_COLO-829-BL/caveman/cov_arr | Bin 0 -> 274937 bytes .../COLO-829_vs_COLO-829-BL/caveman/prob_arr | 1 + .../COLO-829_vs_COLO-829-BL/caveman/splitList | 1 + .../COLO-829_vs_COLO-829-BL.annot.vcf.gz | Bin 0 -> 7193 bytes .../COLO-829_vs_COLO-829-BL.annot.vcf.gz.tbi | 1 + .../COLO-829_vs_COLO-829-BL.flagged.vcf.gz | Bin 0 -> 6487 bytes ...COLO-829_vs_COLO-829-BL.flagged.vcf.gz.tbi | 1 + .../pindel/COLO-829_vs_COLO-829-BL_mt.bam | Bin 0 -> 41156 bytes .../pindel/COLO-829_vs_COLO-829-BL_mt.bam.bai | 1 + .../pindel/COLO-829_vs_COLO-829-BL_mt.bam.md5 | 1 + .../pindel/COLO-829_vs_COLO-829-BL_wt.bam | Bin 0 -> 28767 bytes .../pindel/COLO-829_vs_COLO-829-BL_wt.bam.bai | 1 + .../pindel/COLO-829_vs_COLO-829-BL_wt.bam.md5 | 1 + .../COLO-829_vs_COLO-829-BL.annot.muts.vcf.gz | Bin 0 -> 3006 bytes ...O-829_vs_COLO-829-BL.annot.muts.vcf.gz.tbi | 1 + ...OLO-829_vs_COLO-829-BL.flagged.muts.vcf.gz | Bin 0 -> 2723 bytes ...829_vs_COLO-829-BL.flagged.muts.vcf.gz.tbi | 1 + .../COLO-829_vs_COLO-829-BL.muts.ids.vcf.gz | Bin 0 -> 1795 bytes ...OLO-829_vs_COLO-829-BL.muts.ids.vcf.gz.tbi | 1 + .../COLO-829_vs_COLO-829-BL.no_analysis.bed | 180 ++++++++++++++++++ .../COLO-829_vs_COLO-829-BL.snps.ids.vcf.gz | Bin 0 -> 5871 bytes ...OLO-829_vs_COLO-829-BL.snps.ids.vcf.gz.tbi | 1 + .../COLO-829_vs_COLO-829-BL/caveman/alg_bean | 4 + .../caveman/caveman.cfg.ini | 14 ++ .../COLO-829_vs_COLO-829-BL/caveman/cov_arr | Bin 0 -> 274937 bytes .../COLO-829_vs_COLO-829-BL/caveman/prob_arr | 1 + .../COLO-829_vs_COLO-829-BL/caveman/splitList | 1 + .../COLO-829_vs_COLO-829-BL.annot.vcf.gz | Bin 0 -> 7154 bytes .../COLO-829_vs_COLO-829-BL.annot.vcf.gz.tbi | 1 + .../COLO-829_vs_COLO-829-BL.flagged.vcf.gz | Bin 0 -> 6443 bytes ...COLO-829_vs_COLO-829-BL.flagged.vcf.gz.tbi | 1 + .../pindel/COLO-829_vs_COLO-829-BL_mt.bam | Bin 0 -> 41156 bytes .../pindel/COLO-829_vs_COLO-829-BL_mt.bam.csi | Bin 0 -> 195 bytes .../pindel/COLO-829_vs_COLO-829-BL_mt.bam.md5 | 1 + .../pindel/COLO-829_vs_COLO-829-BL_wt.bam | Bin 0 -> 28767 bytes .../pindel/COLO-829_vs_COLO-829-BL_wt.bam.csi | Bin 0 -> 202 bytes .../pindel/COLO-829_vs_COLO-829-BL_wt.bam.md5 | 1 + .../COLO-829_vs_COLO-829-BL.annot.muts.vcf.gz | Bin 0 -> 3003 bytes ...O-829_vs_COLO-829-BL.annot.muts.vcf.gz.tbi | 1 + ...OLO-829_vs_COLO-829-BL.flagged.muts.vcf.gz | Bin 0 -> 2721 bytes ...829_vs_COLO-829-BL.flagged.muts.vcf.gz.tbi | 1 + .../COLO-829_vs_COLO-829-BL.muts.ids.vcf.gz | Bin 0 -> 1792 bytes ...OLO-829_vs_COLO-829-BL.muts.ids.vcf.gz.tbi | 1 + .../COLO-829_vs_COLO-829-BL.no_analysis.bed | 180 ++++++++++++++++++ .../COLO-829_vs_COLO-829-BL.snps.ids.vcf.gz | Bin 0 -> 5875 bytes ...OLO-829_vs_COLO-829-BL.snps.ids.vcf.gz.tbi | 1 + .../COLO-829_vs_COLO-829-BL/caveman/alg_bean | 4 + .../caveman/caveman.cfg.ini | 14 ++ .../COLO-829_vs_COLO-829-BL/caveman/cov_arr | Bin 0 -> 274937 bytes .../COLO-829_vs_COLO-829-BL/caveman/prob_arr | 1 + .../COLO-829_vs_COLO-829-BL/caveman/splitList | 1 + .../COLO-829_vs_COLO-829-BL.annot.vcf.gz | Bin 0 -> 7178 bytes .../COLO-829_vs_COLO-829-BL.annot.vcf.gz.tbi | 1 + .../COLO-829_vs_COLO-829-BL.flagged.vcf.gz | Bin 0 -> 6471 bytes ...COLO-829_vs_COLO-829-BL.flagged.vcf.gz.tbi | 1 + .../pindel/COLO-829_vs_COLO-829-BL_mt.cram | Bin 0 -> 27629 bytes .../COLO-829_vs_COLO-829-BL_mt.cram.crai | 1 + .../COLO-829_vs_COLO-829-BL_mt.cram.md5 | 1 + .../pindel/COLO-829_vs_COLO-829-BL_wt.cram | Bin 0 -> 20263 bytes .../COLO-829_vs_COLO-829-BL_wt.cram.crai | 1 + .../COLO-829_vs_COLO-829-BL_wt.cram.md5 | 1 + 75 files changed, 709 insertions(+), 1 deletion(-) create mode 100644 examples/README.md create mode 100644 expected/README.md create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.annot.muts.vcf.gz create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.annot.muts.vcf.gz.tbi create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.flagged.muts.vcf.gz create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.flagged.muts.vcf.gz.tbi create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.muts.ids.vcf.gz create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.muts.ids.vcf.gz.tbi create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.no_analysis.bed create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.snps.ids.vcf.gz create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.snps.ids.vcf.gz.tbi create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/caveman/alg_bean create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/caveman/caveman.cfg.ini create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/caveman/cov_arr create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/caveman/prob_arr create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/caveman/splitList create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL.annot.vcf.gz create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL.annot.vcf.gz.tbi create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL.flagged.vcf.gz create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL.flagged.vcf.gz.tbi create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_mt.bam create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_mt.bam.bai create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_mt.bam.md5 create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_wt.bam create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_wt.bam.bai create mode 100644 expected/cgpwxs/bam_bai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_wt.bam.md5 create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.annot.muts.vcf.gz create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.annot.muts.vcf.gz.tbi create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.flagged.muts.vcf.gz create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.flagged.muts.vcf.gz.tbi create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.muts.ids.vcf.gz create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.muts.ids.vcf.gz.tbi create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.no_analysis.bed create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.snps.ids.vcf.gz create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.snps.ids.vcf.gz.tbi create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/caveman/alg_bean create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/caveman/caveman.cfg.ini create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/caveman/cov_arr create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/caveman/prob_arr create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/caveman/splitList create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL.annot.vcf.gz create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL.annot.vcf.gz.tbi create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL.flagged.vcf.gz create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL.flagged.vcf.gz.tbi create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_mt.bam create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_mt.bam.csi create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_mt.bam.md5 create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_wt.bam create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_wt.bam.csi create mode 100644 expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_wt.bam.md5 create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.annot.muts.vcf.gz create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.annot.muts.vcf.gz.tbi create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.flagged.muts.vcf.gz create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.flagged.muts.vcf.gz.tbi create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.muts.ids.vcf.gz create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.muts.ids.vcf.gz.tbi create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.no_analysis.bed create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.snps.ids.vcf.gz create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.snps.ids.vcf.gz.tbi create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/caveman/alg_bean create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/caveman/caveman.cfg.ini create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/caveman/cov_arr create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/caveman/prob_arr create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/caveman/splitList create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL.annot.vcf.gz create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL.annot.vcf.gz.tbi create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL.flagged.vcf.gz create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL.flagged.vcf.gz.tbi create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_mt.cram create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_mt.cram.crai create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_mt.cram.md5 create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_wt.cram create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_wt.cram.crai create mode 100644 expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_wt.cram.md5 diff --git a/README.md b/README.md index f23038a..c1ec108 100644 --- a/README.md +++ b/README.md @@ -20,6 +20,7 @@ associated annotation of VCF files. This has been packaged specifically for use - [Native docker](#native-docker) - [Singularity](#singularity) - [Test data](#test-data) +- [Development environment](#development-environment) - [Release process](#release-process) - [LICENCE](#licence) @@ -116,7 +117,8 @@ via the options of `ds-cgpwxs.pl` The `examples/` contains test data that can be used to verify the tool. -You can find expected outputs on the Sanger Institute FTP site (based on BAM+BAI inputs): [dockstore-cgpwxs-expected.tar.gz][cgpwxs-exp] +You can find expected outputs on the Sanger Institute FTP site (based on BAM+BAI inputs): +[dockstore-cgpwxs-expected.tar.gz][cgpwxs-exp] ## Development environment diff --git a/examples/README.md b/examples/README.md new file mode 100644 index 0000000..0f297e4 --- /dev/null +++ b/examples/README.md @@ -0,0 +1,11 @@ +# Example parameters + +This area reflects the structure of the expected tree. + +Where a `*.json` file resides in examples you will find a corresponding folder here containing the +unpacked result for executing the relevant workflow with that json example. + +e.g. + +`examples/cgpwxs/bam_bai.json` generates `result_WXS_bam_bai.tar.gz`, the content of which can be +compared to `expected/cgpwxs/bam_bai`. diff --git a/expected/README.md b/expected/README.md new file mode 100644 index 0000000..7b8e222 --- /dev/null +++ b/expected/README.md @@ -0,0 +1,67 @@ +# Expected results + + + +- [Structure](#structure) + - [Files of 1 byte](#files-of-1-byte) +- [How to verify results](#how-to-verify-results) + - [cgpwxs](#cgpwxs) +- [Why not a tar.gz file?](#why-not-a-targz-file) + + + +## Structure + +This area reflects the structure of the examples tree. + +Where a `*.json` file resides in examples you will find a corresponding folder here containing the +unpacked result for executing the relevant workflow with that json example. + +e.g. + +`examples/cgpwxs/bam_bai.json` generates `result_WXS_bam_bai.tar.gz`, the content of which can be compared to `expected/cgpwxs/bam_bai`. + +### Files of 1 byte + +We include all files in this area but those that are not considered useful for verification +purposes have been truncated to a size of 1 (newline char only). + +## How to verify results + +### cgpwxs + +The primary files to compare are: + +1. `/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.annot.muts.vcf.gz` + * Annotated substitutions, `muts.ids.vcf.gz` and `flagged.muts.vcf.gz` are precursors, data only being added. +1. `/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL.annot.vcf.gz` + * Annotated indels, `flagged.vcf.gz` is a precursor, data only being added. + +To compare files you need to strip the ID column as it is populated with UUIDs. + + + +``` +$ export DS_CGPWXS_TAG=X.X.X +$ export TOOL=cgpwxs +$ export EXJS=bam_bai + # the following can be looped for different vcf/algs +$ export ALG=caveman +$ export VCF=COLO-829_vs_COLO-829-BL.annot.muts.vcf.gz +$ export ALG_VCF=COLO-829_vs_COLO-829-BL/$ALG/$VCF +$ export YOUR_UNPACKED=.../$ALG_VCF +$ wget https://raw.githubusercontent.com/cancerit/dockstore-cgpwxs/$DS_CGPWXS_TAG/expected/$TOOL/$EXJS/$ALG_VCF +$ zgrep -v '##contig' $VCF | cut --complement -f 3 > expected_${EXJS}_${ALG}.noid +$ zgrep -v '##contig' $YOUR_UNPACKED | cut --complement -f 3 > mine_${EXJS}_${ALG}.noid +$ diff -y --suppress-common-lines expected_${EXJS}.noid mine_${EXJS}.noid + ##vcfProcessLog=,InputVCFSource=,InputV | ##vcfProcessLog=,InputVCFSource=,InputV + ##vcfProcessLog_20180307.1=,InputVCFSource=,InputVCFSource={>=Ug*Up#SI>(XZ_avSx1`>v< zKv?WBdwX@2GDd*wjLq66v$>ide=WZdaAKik=c@L@B_Uv0{dBAQQLAO~;lnbF=rTz+ ze(ubAH;W_asePo1k_-|2gwdj~ zk0Kh;O!`VwDt&Y$qDyl>qtfp?KMM1wA3q+0PAA8hz2uRm{)$Rp{Y;AX3M|Qw7gC(O zs%4q%;AR*@SGWk%ESCrvlq@A*N~yn)e#+M2GN%g(DaewRX|gH0X1Sl{1u*?1r}3ih zut@;4P+P&U&IM3J<5j-?xjBf@_;?VbJpkYp2o1C0UcUDbKK9V@a3C7NnzEroV4|kt ziPunktOnktB!)jKEy9!rtP``QbP7%vtVi+{;92{2n`B|m+>(M%el8pXR%A3t;M3ZV zmWRGjB=`760SYa-AI4c}dyC8!x(n{@w%aHKN6g{HX5Nd?ettEym1&GRRb 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diff --git a/expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/caveman/caveman.cfg.ini b/expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/caveman/caveman.cfg.ini new file mode 100644 index 0000000..09f2342 --- /dev/null +++ b/expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/caveman/caveman.cfg.ini @@ -0,0 +1,14 @@ +CWD=/var/spool/cwl +MUT_BAM=/var/spool/cwl/tmp/COLO-829.bam +NORM_BAM=/var/spool/cwl/tmp/COLO-829-BL.bam +REF_IDX=/var/spool/cwl/reference_files/genome.fa.fai +IGNORE=/var/spool/cwl/reference_files/caveman/HiDepth.tsv +ALG_FILE=/var/spool/cwl/COLO-829_vs_COLO-829-BL/caveman/tmpCaveman/alg_bean +RESULT_DIR=/var/spool/cwl/COLO-829_vs_COLO-829-BL/caveman/tmpCaveman/results +SPLIT_FILE=/var/spool/cwl/COLO-829_vs_COLO-829-BL/caveman/tmpCaveman/splitList +SW=0 +SE=0 +DUP=0 +NORMCN=/var/spool/cwl/tmp/empty.cn.bed +TUMCN=/var/spool/cwl/tmp/empty.cn.bed +VER=1.13.0 diff --git a/expected/cgpwxs/bam_csi/COLO-829_vs_COLO-829-BL/caveman/cov_arr 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100644 index 0000000..8b13789 --- /dev/null +++ b/expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.snps.ids.vcf.gz.tbi @@ -0,0 +1 @@ + diff --git a/expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/caveman/alg_bean b/expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/caveman/alg_bean new file mode 100644 index 0000000..0fb9883 --- /dev/null +++ b/expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/caveman/alg_bean @@ -0,0 +1,4 @@ +rd_pos 2.63;47.37;23.68;13.16;13.16 +base_qual 0-9;10-19;20-24;25-29;30-34;35-39;40-200 +map_qual 0-60;255-255;61-254 +lane 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diff --git a/expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/caveman/caveman.cfg.ini b/expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/caveman/caveman.cfg.ini new file mode 100644 index 0000000..8fd6d80 --- /dev/null +++ b/expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/caveman/caveman.cfg.ini @@ -0,0 +1,14 @@ +CWD=/var/spool/cwl +MUT_BAM=/var/spool/cwl/tmp/COLO-829.cram +NORM_BAM=/var/spool/cwl/tmp/COLO-829-BL.cram +REF_IDX=/var/spool/cwl/reference_files/genome.fa.fai +IGNORE=/var/spool/cwl/reference_files/caveman/HiDepth.tsv +ALG_FILE=/var/spool/cwl/COLO-829_vs_COLO-829-BL/caveman/tmpCaveman/alg_bean +RESULT_DIR=/var/spool/cwl/COLO-829_vs_COLO-829-BL/caveman/tmpCaveman/results +SPLIT_FILE=/var/spool/cwl/COLO-829_vs_COLO-829-BL/caveman/tmpCaveman/splitList +SW=0 +SE=0 +DUP=0 +NORMCN=/var/spool/cwl/tmp/empty.cn.bed +TUMCN=/var/spool/cwl/tmp/empty.cn.bed +VER=1.13.0 diff --git a/expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/caveman/cov_arr 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a/expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_wt.cram.crai b/expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_wt.cram.crai new file mode 100644 index 0000000..8b13789 --- /dev/null +++ b/expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_wt.cram.crai @@ -0,0 +1 @@ + diff --git a/expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_wt.cram.md5 b/expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_wt.cram.md5 new file mode 100644 index 0000000..8b13789 --- /dev/null +++ b/expected/cgpwxs/cram_crai/COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_wt.cram.md5 @@ -0,0 +1 @@ + From 655364c21d5df4cafa1b465ad91c606d8a3d84f6 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Thu, 8 Mar 2018 18:54:08 +0000 Subject: [PATCH 50/54] Expand docs, add links [ci skip] --- README.md | 10 +++++----- examples/README.md | 13 ++++++++----- expected/README.md | 7 +------ 3 files changed, 14 insertions(+), 16 deletions(-) diff --git a/README.md b/README.md index c1ec108..bfd5e1c 100644 --- a/README.md +++ b/README.md @@ -19,7 +19,7 @@ associated annotation of VCF files. This has been packaged specifically for use - [Other uses](#other-uses) - [Native docker](#native-docker) - [Singularity](#singularity) - - [Test data](#test-data) +- [Verifying your deployment](#verifying-your-deployment) - [Development environment](#development-environment) - [Release process](#release-process) - [LICENCE](#licence) @@ -113,12 +113,12 @@ By default results are written to the home directory of the container so ensure a large volume and set the `-home` variable. As indicated above the location can be overridden via the options of `ds-cgpwxs.pl` -### Test data +## Verifying your deployment -The `examples/` contains test data that can be used to verify the tool. +The `examples/` tree contains test json files populated with data that can be used to verify the +tool. More details can be found [here](examples/README.md). -You can find expected outputs on the Sanger Institute FTP site (based on BAM+BAI inputs): -[dockstore-cgpwxs-expected.tar.gz][cgpwxs-exp] +The `expected/` tree contains the expected output for each tool. More details can be found [here](expected/README.md). ## Development environment diff --git a/examples/README.md b/examples/README.md index 0f297e4..0e6a9c8 100644 --- a/examples/README.md +++ b/examples/README.md @@ -1,11 +1,14 @@ # Example parameters -This area reflects the structure of the expected tree. +An example json file exists for the various combinations of sequence input file (and index). -Where a `*.json` file resides in examples you will find a corresponding folder here containing the -unpacked result for executing the relevant workflow with that json example. +For each `examples//.json` file there is a corresponding folder named +`expected///` containing the expected file listing for the result archive. e.g. -`examples/cgpwxs/bam_bai.json` generates `result_WXS_bam_bai.tar.gz`, the content of which can be -compared to `expected/cgpwxs/bam_bai`. +* JSON: `examples/cgpwxs/bam_bai.json` + * Generates archive: `result_WXS_bam_bai.tar.gz` +* Unpacked archive can be compared to: `expected/cgpwxs/bam_bai` + +See `expected/` [docs](../expected/README.md) for content of that area. diff --git a/expected/README.md b/expected/README.md index 7b8e222..f235a99 100644 --- a/expected/README.md +++ b/expected/README.md @@ -14,12 +14,7 @@ This area reflects the structure of the examples tree. -Where a `*.json` file resides in examples you will find a corresponding folder here containing the -unpacked result for executing the relevant workflow with that json example. - -e.g. - -`examples/cgpwxs/bam_bai.json` generates `result_WXS_bam_bai.tar.gz`, the content of which can be compared to `expected/cgpwxs/bam_bai`. +See `examples/` [docs](../examples/README.md) for correlation to json param files. ### Files of 1 byte From b258a891417d8c41cfc8adbea288fcaec75b9d30 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Thu, 8 Mar 2018 19:00:38 +0000 Subject: [PATCH 51/54] Expand deployment docs [ci skip] --- README.md | 3 +++ 1 file changed, 3 insertions(+) diff --git a/README.md b/README.md index bfd5e1c..3d0411b 100644 --- a/README.md +++ b/README.md @@ -137,6 +137,9 @@ git config core.hooksPath git-hooks This project is maintained using HubFlow. 1. Make appropriate changes +1. Build image locally +1. Run all example inputs and verify any changes are acceptable +1. Update `expected/` tree with output of last step 1. Bump version in `Dockerfile` and `Dockstore.cwl` 1. Push changes 1. Check state on Travis From f7c568a5504b3a9353ec4ed604c33f23b7fb5586 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Thu, 8 Mar 2018 19:00:57 +0000 Subject: [PATCH 52/54] Modify links [ci skip] --- cwls/cgpwxs.cwl | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/cwls/cgpwxs.cwl b/cwls/cgpwxs.cwl index 21bbbae..004d9bf 100644 --- a/cwls/cgpwxs.cwl +++ b/cwls/cgpwxs.cwl @@ -10,7 +10,7 @@ cwlVersion: v1.0 doc: | ![build_status](https://quay.io/repository/wtsicgp/dockstore-cgpwxs/status) - A Docker container for the CGP WXS analysis flow. See the [dockstore-cgpwxs](https://github.com/cancerit/dockstore-cgpwxs) website for more information. + A Docker container for the CGP WXS analysis flow. See the [dockstore-cgpwxs](https://github.com/cancerit/dockstore-cgpwxs/blob/master/README.md) website for more information. Please read the relevant [changes](https://github.com/cancerit/dockstore-cgpwxs/blob/master/CHANGES.md) when upgrading. From e0559ed44a89244982c6c02a9bfb19f1fc3fd38e Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Fri, 9 Mar 2018 14:03:52 +0000 Subject: [PATCH 53/54] Minor change to show more representative lines of header that may change when comparing --- expected/README.md | 9 ++++++--- 1 file changed, 6 insertions(+), 3 deletions(-) diff --git a/expected/README.md b/expected/README.md index f235a99..64d4462 100644 --- a/expected/README.md +++ b/expected/README.md @@ -49,12 +49,15 @@ $ wget https://raw.githubusercontent.com/cancerit/dockstore-cgpwxs/$DS_CGPWXS_TA $ zgrep -v '##contig' $VCF | cut --complement -f 3 > expected_${EXJS}_${ALG}.noid $ zgrep -v '##contig' $YOUR_UNPACKED | cut --complement -f 3 > mine_${EXJS}_${ALG}.noid $ diff -y --suppress-common-lines expected_${EXJS}.noid mine_${EXJS}.noid + ##cavemanVersion=1.12.1 | ##cavemanVersion=1.13.0 + ##fileDate=20180302 | ##fileDate=20180309 + ##source_20180302.1=AnnotateVcf.pl | ##source_20180309.1=AnnotateVcf.pl ##vcfProcessLog=,InputVCFSource=,InputV | ##vcfProcessLog=,InputVCFSource=,InputV - ##vcfProcessLog_20180307.1=,InputVCFSource=,InputVCFSource=,InputVCFSource=,InputVCFSource= Date: Fri, 9 Mar 2018 16:01:52 +0000 Subject: [PATCH 54/54] Bump the versions and do local tests to update expected datasets [ci skip] --- CHANGES.md | 2 +- Dockerfile | 2 +- cwls/cgpwxs.cwl | 2 +- 3 files changed, 3 insertions(+), 3 deletions(-) diff --git a/CHANGES.md b/CHANGES.md index d2a7872..832bb6d 100644 --- a/CHANGES.md +++ b/CHANGES.md @@ -1,6 +1,6 @@ # CHANGES -## NEXT +## 3.0.0 * See dockstore-cgpmap v3.0.0 (primarily adds mismatchQc) * CaVEMan and cgpPindel updated to use fragment based counting. diff --git a/Dockerfile b/Dockerfile index fe41431..cdd2767 100644 --- a/Dockerfile +++ b/Dockerfile @@ -33,7 +33,7 @@ MAINTAINER cgphelp@sanger.ac.uk LABEL vendor="CASM/Cancer IT, Wellcome Sanger Institute" LABEL uk.ac.sanger.cgp.description="CGP WXS pipeline for dockstore.org" -LABEL uk.ac.sanger.cgp.version="3.0.0-rc1" +LABEL uk.ac.sanger.cgp.version="3.0.0" RUN apt-get -yq update RUN apt-get install -yq --no-install-recommends\ diff --git a/cwls/cgpwxs.cwl b/cwls/cgpwxs.cwl index 004d9bf..829bfe5 100644 --- a/cwls/cgpwxs.cwl +++ b/cwls/cgpwxs.cwl @@ -24,7 +24,7 @@ doc: | requirements: - class: DockerRequirement - dockerPull: "quay.io/wtsicgp/dockstore-cgpwxs:3.0.0-rc2" + dockerPull: "quay.io/wtsicgp/dockstore-cgpwxs:3.0.0" hints: - class: ResourceRequirement