Two questions; about 'Cell_type_noise' filter and MT mutation calling

[leehs96]

Hello,

I hope you're doing well. I'm currently working with 3'-10X chromium scRNA data, and I have a couple of questions regarding the filtering steps.

'Cell_type_noise' filter: I would appreciate it if you could provide more details on the meaning of the 'Cell_type_noise' filter. My understanding is that it might be related to mutations detected across several cell types, but I noticed it's annotated with the name 'Multiple_cell_types.' Could you clarify the exact significance of 'Cell_type_noise' in this context?

Alternative filtering for MT mutation calling: I'm particularly interested in understanding MT mutation accumulation in specific cell types in the context of cancer. Considering this, I'm a bit unsure whether I need to adjust the filtering criteria from the examples provided. Do you have any recommendations for filtering criteria specifically tailored for MT mutation calling in this scenario?

I would greatly appreciate any insights or recommendations you could share. Thank you in advance.

[nikonovaelizaveta]

I would also like to get clarification on filters. Could you please post the meaning of each of the filters.