Genome assemblies of four Fundulus killifish with ONT and Illumina

2019

https://www.biorxiv.org/content/10.1101/686246v1

Lisa K. Johnson [1,2], Ruta Sahasrabudhe [3], Tony Gill [1], Jennifer Roach [1], Lutz Froenicke [3], C. Titus Brown [2], Andrew Whitehead* [1]

[1] Department of Environmental Toxicology, University of California, Davis [2] Department of Population Health & Reproduction, School of Veterinary Medicine, University of California, Davis [3] DNA Technologies Core, Genome Center, University of California, Davis *corresponding author: awhitehead@ucdavis.edu

Workflow

1. QC scripts
2. Assembly scripts
3. Evaluation scripts

License: MIT

Data

Raw ONT and Illumina reads: https://www.ebi.ac.uk/ena/data/view/PRJEB29136

Johnson, L. (2019, June 21). Genome assemblies of killifish from the Fundulus genus with ONT. https://doi.org/10.17605/osf.io/zjv86

Johnson, Lisa K., Brown, C. Titus, & Whitehead, Andrew. (2019). Draft genome assemblies of killifish from the Fundulus genus with ONT and Illumina sequencing platforms [Data set]. Zenodo. http://doi.org/10.5281/zenodo.3251034

Assembly evaluations and other stats:

Johnson, L. (2019, June 28). Genome assemblies of killifish from the Fundulus genus with ONT. https://doi.org/10.17605/osf.io/zjv86

Thank you!

• TAMU seq facility, Charlie Johnson, Richard Metz, Joshua Hill. Hosted Porecamp, then they offered us free NovaSeq!
• Porecamp: Nick Loman, Joshua Quick, Mick Watson, Matt Loose, John Tyson
• David Duvernell, Southern Illinois State University and Jacob Shaefer for generously providing Fundulus fish