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Merge pull request #338 from Delphine-L/vgp6b
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 addition of workflow  Vgp6b for the purging of a single haplotype
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mvdbeek authored Feb 14, 2024
2 parents 49525aa + 1ca8368 commit f5a558f
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version: 1.2
workflows:
- name: main
subclass: Galaxy
publish: true
primaryDescriptorPath: /Purging-duplicates-one-haplotype-VGP6b.ga
testParameterFiles:
- /Purging-duplicates-one-haplotype-VGP6b-tests.yml
authors:
- name: Galaxy
- name: VGP
url: https://vertebrategenomeproject.org
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# Changelog

## [0.1] - 2024-02-07

### Added

- Workflow for purging duplication in contigs in single haplotype
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- doc: Test outline for Purging-duplicates-one-haplotype-VGP6b
job:
Genomescope model parameters:
class: File
location: https://zenodo.org/records/10632760/files/Genomescope%20model%20parameters.tabular?download=1
filetype: tabular
Assembly to purge:
class: File
location: https://zenodo.org/records/10632760/files/hap1.fasta?download=1
filetype: fasta
Meryl Database:
class: File
location: https://zenodo.org/records/10632760/files/Meryl%20Database.meryldb?download=1
filetype: meryldb
Estimated genome size - Parameter File:
class: File
location: https://zenodo.org/records/10632760/files/Estimated%20genome%20size%20-%20Parameter%20File.expression.json?download=1
filetype: expression.json
Assembly to leave alone (need this for merqury):
class: File
location: https://zenodo.org/records/10632760/files/hap2.fasta?download=1
filetype: fasta
Pacbio Reads Collection - Trimmed:
class: Collection
collection_type: list
elements:
- class: File
identifier: yeast_reads_sub1.fastq.gz
location: https://zenodo.org/records/10632760/files/Trimmed_yeast_reads_sub1.fastq.gz?download=1
Name of un-altered assembly: Hap2
Name of purged assembly: Hap1
outputs:
Removed haplotigs:
asserts:
has_n_lines:
n: 14
Purged assembly:
asserts:
has_n_lines:
n: 158
Purged assembly (GFA):
asserts:
has_n_lines:
n: 160
Assembly statistics:
asserts:
has_text:
text: "Average contig length 23,851.86 24,476.28"
Cutoffs:
asserts:
has_text:
text: "48"
'Busco on Purged Primary assembly: short summary':
asserts:
has_text:
text: "C:1.1%[S:1.1%,D:0.0%],F:0.5%,M:98.4%,n:3354"
Purged assembly statistics:
asserts:
has_text:
text: "# scaffolds 79"
Nx Plot:
asserts:
has_size:
value : 57000
delta: 5000
Size Plot:
asserts:
has_size:
value : 84000
delta: 5000
'Merqury on Phased assemblies: stats':
element_tests:
output_merqury.completeness:
asserts:
has_text:
text: "95.7636"
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