From c13c0264f07026fb5f5e7ef56839e28acd717f41 Mon Sep 17 00:00:00 2001 From: Louis Cahill Date: Wed, 11 Oct 2023 17:07:26 +0000 Subject: [PATCH] fixed bio photos --- pages/mdx/biology.mdx | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/pages/mdx/biology.mdx b/pages/mdx/biology.mdx index 827fbe2d..52c19b1a 100644 --- a/pages/mdx/biology.mdx +++ b/pages/mdx/biology.mdx @@ -143,7 +143,7 @@ of an expensive thermocycler. More information on the probes used can be found o Extraction of miRNA is done using electromagnetic particles. These are the steps: Samples of blood are taken from a patient and added to a solution containing magnetic nanoparticles. These nanoparticles often consist of a metal (usually iron) oxide and are also coated with a biotin-streptavidin bonded anti-miRNA strand. A set of electromagnets is turned on and off in quick succession, causing the nanoparticles to move and stir the solution. During this process, target miRNAs bond to the complementary anti-miRNA via base-pairing. Afterwards, the magnetic beads are pulled to the side of the container while the rest of the solution is removed. The miRNA is removed from the beads using an elution buffer. The previous steps are repeated three times in order to remove the maximum possible amount of miRNA for amplification. @@ -160,7 +160,7 @@ Two DNA probes, one with 5’ phosphorylation, bind to the miRNA, and are ligate In order for the miRNA to be detected, we use ‘asymmetric RPA’: an excess of forward primers are added (usually 5x the amount), so an excess of the strand that was originally miRNA form is produced, so there is now ssDNA with the miRNA code in DNA. This can be detected by toehold switches. In order to design probes for miRPA, we can use NUPACK’s design functions in its API to find probes which can bind to the miRNAs, but have overhangs which do not bind within themselves, to ensure primers can easily anneal to them.

Step 3: Charaterisation of toehold switches

@@ -172,7 +172,7 @@ The RNA and gates are specially designed to join miRNA strands for a single dise As seen earlier, our switches are multiplexed allowing us to detect 4 diseases at once. Each switch has their own binding site and reporter protein depending on the condition the switch is designed to detect.