updating notebook

hputnam · Aug 28, 2023 · bfe6e69 · bfe6e69
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diff --git a/_posts/2023-07-23-PocID.md b/_posts/2023-07-23-PocID.md
@@ -291,7 +291,10 @@ MIC will sequence the first 4 Forward and the first 4 reverse to check sequencin
 All sequences were completed in Forward and Reverse
 
 # Geneious Prime analysis of mtORF
-Analysis of data indicated 3 samples were Haplotype 8a and 14 samples were Haplotype 1a, which could be Pocillopora meandrina or Pocillopora grandis. PocHistone PCR and RFLP was used to identify the species for Haplotype 1a according to [Johnston et al. 2018](https://peerj.com/articles/4355/) 
+Analysis of data indicated 3 samples were Haplotype 8a and 14 samples were Haplotype 1a, which could be Pocillopora meandrina or Pocillopora grandis. PocHistone PCR and RFLP was used to identify the species for Haplotype 1a according to [Johnston et al. 2018](https://peerj.com/articles/4355/)  
+
+[Raw seqeunce data, refernece sequences, and alignments](https://osf.io/5geqd/)  
+![mtORF Neighborjoining Tree]() 
 
 # PocHistone PCR
 ## 15 August 2023
@@ -332,10 +335,10 @@ Ran PocHIstone RFLP with NEB Restriction Enzyme XhoI, NEB Cat# R0146S
 Reagent	|1Rxn µl	|20 Rxn µl|   
 ---|---|---|   
 XhoI restriction enzyme |	0.5	|10 |  
-1X CutSmart⃝R buffer 	|0.5|	10 |  
+1X CutSmartR buffer 	|0.5|	10 |  
 
 For each sample added 1µl of Restriction Digest Master Mix to 15µl of PCR product.
-Incubated samples for 1 hr at 37 ◦C followed by 20 min at 65 ◦C to inactivate the enzymes. 
+Incubated samples for 1 hr at 37°C followed by 20 min at 65°C to inactivate the enzymes. 
 
 
 ## PCR Gel protocol

diff --git a/images/20230723_Poc_mtORF_Tree.png b/images/20230723_Poc_mtORF_Tree.png