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updated analysis docs for cellranger-arc
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avikdatta committed Feb 8, 2024
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<td><a href="https://www.10xgenomics.com/support/software/cell-ranger">Cellranger</a></td>
<td>Active</td>
</tr>
<tr>
<td><a href="#cellranger-arc">Single cell Gene expression and ATAC-Seq multiome data analysis for 10X genomics library</a></td>
<td><a href="https://support.10xgenomics.com/single-cell-multiome-atac-gex/software/overview/welcomer">Cellranger-ARC</a></td>
<td>Active</td>
</tr>
<tr>
<td><a href="#nf-core_atacseq">ATAC-Seq alignment, peak calling and QC</a></td>
<td><a href="https://nf-co.re/atacseq">nf-core/atacseq</a></td>
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</table>
</div>
<div align="right"><a href="#table-of-contents">Go to Top</a></div>
<div>
<h2 id="cellranger-arc">Single cell Gene expression and ATAC-Seq multiome data analysis for 10X genomics library</h2>
<table class="table" style="border:hidden;">
<thead>
</thead>
<tbody>
<tr>
<td style="border:hidden; width:25%"><b>Pipeline name:</b></td>
<td style="border:hidden;"><a href="https://support.10xgenomics.com/single-cell-multiome-atac-gex/software/overview/welcome">Cellranger-ARC</a></td>
</tr>
<tr>
<td style="border:hidden; width:25%"><b>Supported versions:</b></td>
<td style="border:hidden;">Latest (>= 2.0.2)</td>
</tr>
<tr>
<td style="border:hidden; width:25%"><b>Required inputs:</b></td>
<td style="border:hidden;">
<p>We need following details to configure and run this pipeline:</p>
<ul>
<li>List of sample IGF ids and their library type, i.e. Gene Expression or Chromatin Accessibility</li>
<li>Cellranger-ARC group information</li>
<li>Reference genome information, (default ref genome from 10X genomics)</li>
</ul>
<details>
<summary>Click here for more information</summary><p/>
<p><b>List of sample IGF ids and library type</b>. For e.g.,</p>
<pre style="background-color:#E8E8E8"> #igf_id,feature_types,cellranger_group
IGF001,Gene Expression,Group1
IGF002,Chromatin Accessibility,Group1
IGF003,Gene Expression,Group2
IGF004,Chromatin Accessibility,Group2</pre>
</details>
</td>
</tr>
<tr>
<td style="border:hidden; width:25%"><b>Output results:</b></td>
<td style="border:hidden;">
<ul>
<li><a href="https://support.10xgenomics.com/single-cell-multiome-atac-gex/software/pipelines/latest/output/overview">Cellranger-ARC output for individual groups</a></li>
<li><a href="https://support.10xgenomics.com/single-cell-multiome-atac-gex/software/pipelines/latest/using/aggr">Output of Cellranger-ARC Aggr</a></li>
<li><a href="https://github.com/imperial-genomics-facility/scanpy-notebook-image/tree/master/templates">Scanpy QC report for individual and aggregated samples</a></li>
</ul>
</td>
</tr>
</tbody>
</table>
</div>
<div align="right"><a href="#table-of-contents">Go to Top</a></div>
<div>
<h2 id="nf-core_atacseq">NF-core ATAC-Seq workflow</h2>
<table class="table" style="border:hidden;">
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<p><b>List of NF-core ATAC-Seq pipeline parameters</b>. For e.g.,</p>
<pre style="background-color:#E8E8E8"> --trim_nextseq 20
--aligner bwa
--narrow_peak</pre>
--narrow_peak</pre>
</details>
</td>
</tr>
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