MAPS_1_img-QC.ipynb

{
 "cells": [
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "# MAPS Step 1 - Image Quality Control\n",
    "After collecting images, follow the steps below to remove out-of-focus images. Artifacts such as foreign objects, air bubbles and over-expressed cells will be eliminated when measuring focus. <br>\n",
    "Some outputs were saved to show the expected output."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 1,
   "metadata": {},
   "outputs": [],
   "source": [
    "import os\n",
    "import cv2\n",
    "from matplotlib import pyplot as plt\n",
    "import numpy as np\n",
    "from MAPS_util import *"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "## Set the working directory"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 2,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "'/Users/jessesnaust/Documents/python scripts/MAPS_util'"
      ]
     },
     "execution_count": 2,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# check current directory\n",
    "os.getcwd()"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 8,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "'/Volumes/WD_1TB/Cellomics_images'"
      ]
     },
     "execution_count": 8,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# change directory, if needed\n",
    "os.chdir('/volumes/WD_1TB/Cellomics_images/')\n",
    "# confirm the directory\n",
    "os.getcwd()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "## Perform image QC\n",
    "### Note: This program assumes there are 3 channels per field. <br> Therefore, 1 image set has blue, green and red channels.\n",
    "For example: blue-DAPI, green-GFP, red-TRITC.\n",
    "Each channel is saved as separate files with suffixes d0, d1 and d2."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 32,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "Created folder 'not-blurry'.\n"
     ]
    }
   ],
   "source": [
    "folder_path = \"K342N-102319/\" # source\n",
    "\n",
    "create_folder(folder_path, \"not-blurry\") # create destination folder, if it doesn't already exist\n",
    "dst_folder = folder_path + \"not-blurry/\" # destination"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 33,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "There are 300 set of images waiting to be processed.\n"
     ]
    }
   ],
   "source": [
    "# get the file names of the images in the folder\n",
    "ID_list = get_names(folder_path)\n",
    "print(f'There are {len(ID_list)} set of images waiting to be processed.')"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 34,
   "metadata": {
    "scrolled": true
   },
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "\n",
      "Processing image set 1 out of 300. ID=191023110002_C01f100\n",
      "blue: MFGTMP_191023110002_C01f100d0.TIF\n",
      "green: MFGTMP_191023110002_C01f100d1.TIF\n",
      "red: MFGTMP_191023110002_C01f100d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1708.20\n",
      "Image is sharp! 2nd focus check FM2=386.71\n",
      "Processed image saved as merged_191023110002_C01f100.TIF \n",
      "\n",
      "\n",
      "Processing image set 2 out of 300. ID=191023110002_C01f101\n",
      "blue: MFGTMP_191023110002_C01f101d0.TIF\n",
      "green: MFGTMP_191023110002_C01f101d1.TIF\n",
      "red: MFGTMP_191023110002_C01f101d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1123.79\n",
      "Image is sharp! 2nd focus check FM2=464.10\n",
      "Processed image saved as merged_191023110002_C01f101.TIF \n",
      "\n",
      "\n",
      "Processing image set 3 out of 300. ID=191023110002_C01f102\n",
      "blue: MFGTMP_191023110002_C01f102d0.TIF\n",
      "green: MFGTMP_191023110002_C01f102d1.TIF\n",
      "red: MFGTMP_191023110002_C01f102d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=516.32\n",
      "Image is sharp! 2nd focus check FM2=286.13\n",
      "Processed image saved as merged_191023110002_C01f102.TIF \n",
      "\n",
      "\n",
      "Processing image set 4 out of 300. ID=191023110002_C01f103\n",
      "blue: MFGTMP_191023110002_C01f103d0.TIF\n",
      "green: MFGTMP_191023110002_C01f103d1.TIF\n",
      "red: MFGTMP_191023110002_C01f103d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 5 out of 300. ID=191023110002_C01f104\n",
      "blue: MFGTMP_191023110002_C01f104d0.TIF\n",
      "green: MFGTMP_191023110002_C01f104d1.TIF\n",
      "red: MFGTMP_191023110002_C01f104d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1215.82\n",
      "Image is sharp! 2nd focus check FM2=642.45\n",
      "Processed image saved as merged_191023110002_C01f104.TIF \n",
      "\n",
      "\n",
      "Processing image set 6 out of 300. ID=191023110002_C01f105\n",
      "blue: MFGTMP_191023110002_C01f105d0.TIF\n",
      "green: MFGTMP_191023110002_C01f105d1.TIF\n",
      "red: MFGTMP_191023110002_C01f105d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1119.51\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 7 out of 300. ID=191023110002_C01f106\n",
      "blue: MFGTMP_191023110002_C01f106d0.TIF\n",
      "green: MFGTMP_191023110002_C01f106d1.TIF\n",
      "red: MFGTMP_191023110002_C01f106d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1608.36\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 8 out of 300. ID=191023110002_C01f107\n",
      "blue: MFGTMP_191023110002_C01f107d0.TIF\n",
      "green: MFGTMP_191023110002_C01f107d1.TIF\n",
      "red: MFGTMP_191023110002_C01f107d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=563.01\n",
      "Image is sharp! 2nd focus check FM2=265.23\n",
      "Processed image saved as merged_191023110002_C01f107.TIF \n",
      "\n",
      "\n",
      "Processing image set 9 out of 300. ID=191023110002_C01f108\n",
      "blue: MFGTMP_191023110002_C01f108d0.TIF\n",
      "green: MFGTMP_191023110002_C01f108d1.TIF\n",
      "red: MFGTMP_191023110002_C01f108d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 10 out of 300. ID=191023110002_C01f109\n",
      "blue: MFGTMP_191023110002_C01f109d0.TIF\n",
      "green: MFGTMP_191023110002_C01f109d1.TIF\n",
      "red: MFGTMP_191023110002_C01f109d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 11 out of 300. ID=191023110002_C01f110\n",
      "blue: MFGTMP_191023110002_C01f110d0.TIF\n",
      "green: MFGTMP_191023110002_C01f110d1.TIF\n",
      "red: MFGTMP_191023110002_C01f110d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 12 out of 300. ID=191023110002_C01f111\n",
      "blue: MFGTMP_191023110002_C01f111d0.TIF\n",
      "green: MFGTMP_191023110002_C01f111d1.TIF\n",
      "red: MFGTMP_191023110002_C01f111d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=539.20\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 13 out of 300. ID=191023110002_C01f112\n",
      "blue: MFGTMP_191023110002_C01f112d0.TIF\n",
      "green: MFGTMP_191023110002_C01f112d1.TIF\n",
      "red: MFGTMP_191023110002_C01f112d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=757.42\n",
      "Image is sharp! 2nd focus check FM2=212.83\n",
      "Processed image saved as merged_191023110002_C01f112.TIF \n",
      "\n",
      "\n",
      "Processing image set 14 out of 300. ID=191023110002_C01f113\n",
      "blue: MFGTMP_191023110002_C01f113d0.TIF\n",
      "green: MFGTMP_191023110002_C01f113d1.TIF\n",
      "red: MFGTMP_191023110002_C01f113d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=621.43\n",
      "Image is sharp! 2nd focus check FM2=573.49\n",
      "Processed image saved as merged_191023110002_C01f113.TIF \n",
      "\n",
      "\n",
      "Processing image set 15 out of 300. ID=191023110002_C01f114\n",
      "blue: MFGTMP_191023110002_C01f114d0.TIF\n",
      "green: MFGTMP_191023110002_C01f114d1.TIF\n",
      "red: MFGTMP_191023110002_C01f114d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1768.79\n",
      "Image is sharp! 2nd focus check FM2=211.09\n",
      "Processed image saved as merged_191023110002_C01f114.TIF \n",
      "\n",
      "\n",
      "Processing image set 16 out of 300. ID=191023110002_C01f115\n",
      "blue: MFGTMP_191023110002_C01f115d0.TIF\n",
      "green: MFGTMP_191023110002_C01f115d1.TIF\n",
      "red: MFGTMP_191023110002_C01f115d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 17 out of 300. ID=191023110002_C01f116\n",
      "blue: MFGTMP_191023110002_C01f116d0.TIF\n",
      "green: MFGTMP_191023110002_C01f116d1.TIF\n",
      "red: MFGTMP_191023110002_C01f116d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 18 out of 300. ID=191023110002_C01f117\n",
      "blue: MFGTMP_191023110002_C01f117d0.TIF\n",
      "green: MFGTMP_191023110002_C01f117d1.TIF\n",
      "red: MFGTMP_191023110002_C01f117d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 19 out of 300. ID=191023110002_C01f118\n",
      "blue: MFGTMP_191023110002_C01f118d0.TIF\n",
      "green: MFGTMP_191023110002_C01f118d1.TIF\n",
      "red: MFGTMP_191023110002_C01f118d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 20 out of 300. ID=191023110002_C01f119\n",
      "blue: MFGTMP_191023110002_C01f119d0.TIF\n",
      "green: MFGTMP_191023110002_C01f119d1.TIF\n",
      "red: MFGTMP_191023110002_C01f119d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 21 out of 300. ID=191023110002_C01f120\n",
      "blue: MFGTMP_191023110002_C01f120d0.TIF\n",
      "green: MFGTMP_191023110002_C01f120d1.TIF\n",
      "red: MFGTMP_191023110002_C01f120d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 22 out of 300. ID=191023110002_C01f121\n",
      "blue: MFGTMP_191023110002_C01f121d0.TIF\n",
      "green: MFGTMP_191023110002_C01f121d1.TIF\n",
      "red: MFGTMP_191023110002_C01f121d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 23 out of 300. ID=191023110002_C01f122\n",
      "blue: MFGTMP_191023110002_C01f122d0.TIF\n",
      "green: MFGTMP_191023110002_C01f122d1.TIF\n",
      "red: MFGTMP_191023110002_C01f122d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 24 out of 300. ID=191023110002_C01f123\n",
      "blue: MFGTMP_191023110002_C01f123d0.TIF\n",
      "green: MFGTMP_191023110002_C01f123d1.TIF\n",
      "red: MFGTMP_191023110002_C01f123d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 25 out of 300. ID=191023110002_C01f124\n",
      "blue: MFGTMP_191023110002_C01f124d0.TIF\n",
      "green: MFGTMP_191023110002_C01f124d1.TIF\n",
      "red: MFGTMP_191023110002_C01f124d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 26 out of 300. ID=191023110002_C01f125\n",
      "blue: MFGTMP_191023110002_C01f125d0.TIF\n",
      "green: MFGTMP_191023110002_C01f125d1.TIF\n",
      "red: MFGTMP_191023110002_C01f125d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 27 out of 300. ID=191023110002_C01f126\n",
      "blue: MFGTMP_191023110002_C01f126d0.TIF\n",
      "green: MFGTMP_191023110002_C01f126d1.TIF\n",
      "red: MFGTMP_191023110002_C01f126d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 28 out of 300. ID=191023110002_C01f127\n",
      "blue: MFGTMP_191023110002_C01f127d0.TIF\n",
      "green: MFGTMP_191023110002_C01f127d1.TIF\n",
      "red: MFGTMP_191023110002_C01f127d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=517.56\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 29 out of 300. ID=191023110002_C01f128\n",
      "blue: MFGTMP_191023110002_C01f128d0.TIF\n",
      "green: MFGTMP_191023110002_C01f128d1.TIF\n",
      "red: MFGTMP_191023110002_C01f128d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1716.76\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 30 out of 300. ID=191023110002_C01f129\n",
      "blue: MFGTMP_191023110002_C01f129d0.TIF\n",
      "green: MFGTMP_191023110002_C01f129d1.TIF\n",
      "red: MFGTMP_191023110002_C01f129d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 31 out of 300. ID=191023110002_C01f130\n",
      "blue: MFGTMP_191023110002_C01f130d0.TIF\n",
      "green: MFGTMP_191023110002_C01f130d1.TIF\n",
      "red: MFGTMP_191023110002_C01f130d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 32 out of 300. ID=191023110002_C01f131\n",
      "blue: MFGTMP_191023110002_C01f131d0.TIF\n",
      "green: MFGTMP_191023110002_C01f131d1.TIF\n",
      "red: MFGTMP_191023110002_C01f131d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=547.57\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 33 out of 300. ID=191023110002_C01f132\n",
      "blue: MFGTMP_191023110002_C01f132d0.TIF\n",
      "green: MFGTMP_191023110002_C01f132d1.TIF\n",
      "red: MFGTMP_191023110002_C01f132d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=648.40\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 34 out of 300. ID=191023110002_C01f133\n",
      "blue: MFGTMP_191023110002_C01f133d0.TIF\n",
      "green: MFGTMP_191023110002_C01f133d1.TIF\n",
      "red: MFGTMP_191023110002_C01f133d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 35 out of 300. ID=191023110002_C01f134\n",
      "blue: MFGTMP_191023110002_C01f134d0.TIF\n"
     ]
    },
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "green: MFGTMP_191023110002_C01f134d1.TIF\n",
      "red: MFGTMP_191023110002_C01f134d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 36 out of 300. ID=191023110002_C01f135\n",
      "blue: MFGTMP_191023110002_C01f135d0.TIF\n",
      "green: MFGTMP_191023110002_C01f135d1.TIF\n",
      "red: MFGTMP_191023110002_C01f135d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 37 out of 300. ID=191023110002_C01f136\n",
      "blue: MFGTMP_191023110002_C01f136d0.TIF\n",
      "green: MFGTMP_191023110002_C01f136d1.TIF\n",
      "red: MFGTMP_191023110002_C01f136d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=700.62\n",
      "Image is sharp! 2nd focus check FM2=297.04\n",
      "Processed image saved as merged_191023110002_C01f136.TIF \n",
      "\n",
      "\n",
      "Processing image set 38 out of 300. ID=191023110002_C01f137\n",
      "blue: MFGTMP_191023110002_C01f137d0.TIF\n",
      "green: MFGTMP_191023110002_C01f137d1.TIF\n",
      "red: MFGTMP_191023110002_C01f137d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1486.20\n",
      "Image is sharp! 2nd focus check FM2=380.51\n",
      "Processed image saved as merged_191023110002_C01f137.TIF \n",
      "\n",
      "\n",
      "Processing image set 39 out of 300. ID=191023110002_C01f138\n",
      "blue: MFGTMP_191023110002_C01f138d0.TIF\n",
      "green: MFGTMP_191023110002_C01f138d1.TIF\n",
      "red: MFGTMP_191023110002_C01f138d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1334.64\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 40 out of 300. ID=191023110002_C01f139\n",
      "blue: MFGTMP_191023110002_C01f139d0.TIF\n",
      "green: MFGTMP_191023110002_C01f139d1.TIF\n",
      "red: MFGTMP_191023110002_C01f139d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1273.74\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 41 out of 300. ID=191023110002_C01f140\n",
      "blue: MFGTMP_191023110002_C01f140d0.TIF\n",
      "green: MFGTMP_191023110002_C01f140d1.TIF\n",
      "red: MFGTMP_191023110002_C01f140d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 42 out of 300. ID=191023110002_C01f141\n",
      "blue: MFGTMP_191023110002_C01f141d0.TIF\n",
      "green: MFGTMP_191023110002_C01f141d1.TIF\n",
      "red: MFGTMP_191023110002_C01f141d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1236.22\n",
      "Image is sharp! 2nd focus check FM2=729.02\n",
      "Processed image saved as merged_191023110002_C01f141.TIF \n",
      "\n",
      "\n",
      "Processing image set 43 out of 300. ID=191023110002_C01f142\n",
      "blue: MFGTMP_191023110002_C01f142d0.TIF\n",
      "green: MFGTMP_191023110002_C01f142d1.TIF\n",
      "red: MFGTMP_191023110002_C01f142d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=793.51\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 44 out of 300. ID=191023110002_C01f143\n",
      "blue: MFGTMP_191023110002_C01f143d0.TIF\n",
      "green: MFGTMP_191023110002_C01f143d1.TIF\n",
      "red: MFGTMP_191023110002_C01f143d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1674.71\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 45 out of 300. ID=191023110002_C01f144\n",
      "blue: MFGTMP_191023110002_C01f144d0.TIF\n",
      "green: MFGTMP_191023110002_C01f144d1.TIF\n",
      "red: MFGTMP_191023110002_C01f144d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 46 out of 300. ID=191023110002_C01f145\n",
      "blue: MFGTMP_191023110002_C01f145d0.TIF\n",
      "green: MFGTMP_191023110002_C01f145d1.TIF\n",
      "red: MFGTMP_191023110002_C01f145d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=549.99\n",
      "Image is sharp! 2nd focus check FM2=335.98\n",
      "Processed image saved as merged_191023110002_C01f145.TIF \n",
      "\n",
      "\n",
      "Processing image set 47 out of 300. ID=191023110002_C01f146\n",
      "blue: MFGTMP_191023110002_C01f146d0.TIF\n",
      "green: MFGTMP_191023110002_C01f146d1.TIF\n",
      "red: MFGTMP_191023110002_C01f146d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 48 out of 300. ID=191023110002_C01f147\n",
      "blue: MFGTMP_191023110002_C01f147d0.TIF\n",
      "green: MFGTMP_191023110002_C01f147d1.TIF\n",
      "red: MFGTMP_191023110002_C01f147d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=909.85\n",
      "Image is sharp! 2nd focus check FM2=545.59\n",
      "Processed image saved as merged_191023110002_C01f147.TIF \n",
      "\n",
      "\n",
      "Processing image set 49 out of 300. ID=191023110002_C01f148\n",
      "blue: MFGTMP_191023110002_C01f148d0.TIF\n",
      "green: MFGTMP_191023110002_C01f148d1.TIF\n",
      "red: MFGTMP_191023110002_C01f148d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1438.32\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 50 out of 300. ID=191023110002_C01f149\n",
      "blue: MFGTMP_191023110002_C01f149d0.TIF\n",
      "green: MFGTMP_191023110002_C01f149d1.TIF\n",
      "red: MFGTMP_191023110002_C01f149d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1321.74\n",
      "Image is sharp! 2nd focus check FM2=293.94\n",
      "Processed image saved as merged_191023110002_C01f149.TIF \n",
      "\n",
      "\n",
      "Processing image set 51 out of 300. ID=191023110002_C01f150\n",
      "blue: MFGTMP_191023110002_C01f150d0.TIF\n",
      "green: MFGTMP_191023110002_C01f150d1.TIF\n",
      "red: MFGTMP_191023110002_C01f150d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1733.94\n",
      "Image is sharp! 2nd focus check FM2=507.26\n",
      "Processed image saved as merged_191023110002_C01f150.TIF \n",
      "\n",
      "\n",
      "Processing image set 52 out of 300. ID=191023110002_C01f151\n",
      "blue: MFGTMP_191023110002_C01f151d0.TIF\n",
      "green: MFGTMP_191023110002_C01f151d1.TIF\n",
      "red: MFGTMP_191023110002_C01f151d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 53 out of 300. ID=191023110002_C01f152\n",
      "blue: MFGTMP_191023110002_C01f152d0.TIF\n",
      "green: MFGTMP_191023110002_C01f152d1.TIF\n",
      "red: MFGTMP_191023110002_C01f152d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 54 out of 300. ID=191023110002_C01f153\n",
      "blue: MFGTMP_191023110002_C01f153d0.TIF\n",
      "green: MFGTMP_191023110002_C01f153d1.TIF\n",
      "red: MFGTMP_191023110002_C01f153d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 55 out of 300. ID=191023110002_C01f154\n",
      "blue: MFGTMP_191023110002_C01f154d0.TIF\n",
      "green: MFGTMP_191023110002_C01f154d1.TIF\n",
      "red: MFGTMP_191023110002_C01f154d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 56 out of 300. ID=191023110002_C01f155\n",
      "blue: MFGTMP_191023110002_C01f155d0.TIF\n",
      "green: MFGTMP_191023110002_C01f155d1.TIF\n",
      "red: MFGTMP_191023110002_C01f155d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 57 out of 300. ID=191023110002_C01f156\n",
      "blue: MFGTMP_191023110002_C01f156d0.TIF\n",
      "green: MFGTMP_191023110002_C01f156d1.TIF\n",
      "red: MFGTMP_191023110002_C01f156d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 58 out of 300. ID=191023110002_C01f157\n",
      "blue: MFGTMP_191023110002_C01f157d0.TIF\n",
      "green: MFGTMP_191023110002_C01f157d1.TIF\n",
      "red: MFGTMP_191023110002_C01f157d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1348.28\n",
      "Image is sharp! 2nd focus check FM2=514.83\n",
      "Processed image saved as merged_191023110002_C01f157.TIF \n",
      "\n",
      "\n",
      "Processing image set 59 out of 300. ID=191023110002_C01f158\n",
      "blue: MFGTMP_191023110002_C01f158d0.TIF\n",
      "green: MFGTMP_191023110002_C01f158d1.TIF\n",
      "red: MFGTMP_191023110002_C01f158d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=650.82\n",
      "Image is sharp! 2nd focus check FM2=328.67\n",
      "Processed image saved as merged_191023110002_C01f158.TIF \n",
      "\n",
      "\n",
      "Processing image set 60 out of 300. ID=191023110002_C01f159\n",
      "blue: MFGTMP_191023110002_C01f159d0.TIF\n",
      "green: MFGTMP_191023110002_C01f159d1.TIF\n",
      "red: MFGTMP_191023110002_C01f159d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 61 out of 300. ID=191023110002_C01f15\n",
      "blue: MFGTMP_191023110002_C01f150d0.TIF\n",
      "green: MFGTMP_191023110002_C01f150d1.TIF\n",
      "red: MFGTMP_191023110002_C01f150d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f151d0.TIF\n",
      "green: MFGTMP_191023110002_C01f151d1.TIF\n",
      "red: MFGTMP_191023110002_C01f151d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f152d0.TIF\n",
      "green: MFGTMP_191023110002_C01f152d1.TIF\n",
      "red: MFGTMP_191023110002_C01f152d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f153d0.TIF\n",
      "green: MFGTMP_191023110002_C01f153d1.TIF\n",
      "red: MFGTMP_191023110002_C01f153d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f154d0.TIF\n",
      "green: MFGTMP_191023110002_C01f154d1.TIF\n",
      "red: MFGTMP_191023110002_C01f154d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f155d0.TIF\n",
      "green: MFGTMP_191023110002_C01f155d1.TIF\n",
      "red: MFGTMP_191023110002_C01f155d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f156d0.TIF\n",
      "green: MFGTMP_191023110002_C01f156d1.TIF\n",
      "red: MFGTMP_191023110002_C01f156d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f157d0.TIF\n",
      "green: MFGTMP_191023110002_C01f157d1.TIF\n",
      "red: MFGTMP_191023110002_C01f157d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f158d0.TIF\n",
      "green: MFGTMP_191023110002_C01f158d1.TIF\n",
      "red: MFGTMP_191023110002_C01f158d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f159d0.TIF\n",
      "green: MFGTMP_191023110002_C01f159d1.TIF\n",
      "red: MFGTMP_191023110002_C01f159d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f15d0.TIF\n",
      "green: MFGTMP_191023110002_C01f15d1.TIF\n",
      "red: MFGTMP_191023110002_C01f15d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 62 out of 300. ID=191023110002_C01f160\n",
      "blue: MFGTMP_191023110002_C01f160d0.TIF\n"
     ]
    },
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "green: MFGTMP_191023110002_C01f160d1.TIF\n",
      "red: MFGTMP_191023110002_C01f160d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 63 out of 300. ID=191023110002_C01f161\n",
      "blue: MFGTMP_191023110002_C01f161d0.TIF\n",
      "green: MFGTMP_191023110002_C01f161d1.TIF\n",
      "red: MFGTMP_191023110002_C01f161d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 64 out of 300. ID=191023110002_C01f162\n",
      "blue: MFGTMP_191023110002_C01f162d0.TIF\n",
      "green: MFGTMP_191023110002_C01f162d1.TIF\n",
      "red: MFGTMP_191023110002_C01f162d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 65 out of 300. ID=191023110002_C01f163\n",
      "blue: MFGTMP_191023110002_C01f163d0.TIF\n",
      "green: MFGTMP_191023110002_C01f163d1.TIF\n",
      "red: MFGTMP_191023110002_C01f163d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 66 out of 300. ID=191023110002_C01f164\n",
      "blue: MFGTMP_191023110002_C01f164d0.TIF\n",
      "green: MFGTMP_191023110002_C01f164d1.TIF\n",
      "red: MFGTMP_191023110002_C01f164d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 67 out of 300. ID=191023110002_C01f165\n",
      "blue: MFGTMP_191023110002_C01f165d0.TIF\n",
      "green: MFGTMP_191023110002_C01f165d1.TIF\n",
      "red: MFGTMP_191023110002_C01f165d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 68 out of 300. ID=191023110002_C01f166\n",
      "blue: MFGTMP_191023110002_C01f166d0.TIF\n",
      "green: MFGTMP_191023110002_C01f166d1.TIF\n",
      "red: MFGTMP_191023110002_C01f166d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 69 out of 300. ID=191023110002_C01f167\n",
      "blue: MFGTMP_191023110002_C01f167d0.TIF\n",
      "green: MFGTMP_191023110002_C01f167d1.TIF\n",
      "red: MFGTMP_191023110002_C01f167d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 70 out of 300. ID=191023110002_C01f168\n",
      "blue: MFGTMP_191023110002_C01f168d0.TIF\n",
      "green: MFGTMP_191023110002_C01f168d1.TIF\n",
      "red: MFGTMP_191023110002_C01f168d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 71 out of 300. ID=191023110002_C01f169\n",
      "blue: MFGTMP_191023110002_C01f169d0.TIF\n",
      "green: MFGTMP_191023110002_C01f169d1.TIF\n",
      "red: MFGTMP_191023110002_C01f169d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 72 out of 300. ID=191023110002_C01f16\n",
      "blue: MFGTMP_191023110002_C01f160d0.TIF\n",
      "green: MFGTMP_191023110002_C01f160d1.TIF\n",
      "red: MFGTMP_191023110002_C01f160d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f161d0.TIF\n",
      "green: MFGTMP_191023110002_C01f161d1.TIF\n",
      "red: MFGTMP_191023110002_C01f161d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f162d0.TIF\n",
      "green: MFGTMP_191023110002_C01f162d1.TIF\n",
      "red: MFGTMP_191023110002_C01f162d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f163d0.TIF\n",
      "green: MFGTMP_191023110002_C01f163d1.TIF\n",
      "red: MFGTMP_191023110002_C01f163d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f164d0.TIF\n",
      "green: MFGTMP_191023110002_C01f164d1.TIF\n",
      "red: MFGTMP_191023110002_C01f164d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f165d0.TIF\n",
      "green: MFGTMP_191023110002_C01f165d1.TIF\n",
      "red: MFGTMP_191023110002_C01f165d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f166d0.TIF\n",
      "green: MFGTMP_191023110002_C01f166d1.TIF\n",
      "red: MFGTMP_191023110002_C01f166d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f167d0.TIF\n",
      "green: MFGTMP_191023110002_C01f167d1.TIF\n",
      "red: MFGTMP_191023110002_C01f167d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f168d0.TIF\n",
      "green: MFGTMP_191023110002_C01f168d1.TIF\n",
      "red: MFGTMP_191023110002_C01f168d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f169d0.TIF\n",
      "green: MFGTMP_191023110002_C01f169d1.TIF\n",
      "red: MFGTMP_191023110002_C01f169d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f16d0.TIF\n",
      "green: MFGTMP_191023110002_C01f16d1.TIF\n",
      "red: MFGTMP_191023110002_C01f16d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1829.68\n",
      "Image is sharp! 2nd focus check FM2=390.93\n",
      "Processed image saved as merged_191023110002_C01f16.TIF \n",
      "\n",
      "\n",
      "Processing image set 73 out of 300. ID=191023110002_C01f170\n",
      "blue: MFGTMP_191023110002_C01f170d0.TIF\n",
      "green: MFGTMP_191023110002_C01f170d1.TIF\n",
      "red: MFGTMP_191023110002_C01f170d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 74 out of 300. ID=191023110002_C01f171\n",
      "blue: MFGTMP_191023110002_C01f171d0.TIF\n",
      "green: MFGTMP_191023110002_C01f171d1.TIF\n",
      "red: MFGTMP_191023110002_C01f171d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 75 out of 300. ID=191023110002_C01f172\n",
      "blue: MFGTMP_191023110002_C01f172d0.TIF\n",
      "green: MFGTMP_191023110002_C01f172d1.TIF\n",
      "red: MFGTMP_191023110002_C01f172d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 76 out of 300. ID=191023110002_C01f173\n",
      "blue: MFGTMP_191023110002_C01f173d0.TIF\n",
      "green: MFGTMP_191023110002_C01f173d1.TIF\n",
      "red: MFGTMP_191023110002_C01f173d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 77 out of 300. ID=191023110002_C01f174\n",
      "blue: MFGTMP_191023110002_C01f174d0.TIF\n",
      "green: MFGTMP_191023110002_C01f174d1.TIF\n",
      "red: MFGTMP_191023110002_C01f174d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 78 out of 300. ID=191023110002_C01f175\n",
      "blue: MFGTMP_191023110002_C01f175d0.TIF\n",
      "green: MFGTMP_191023110002_C01f175d1.TIF\n",
      "red: MFGTMP_191023110002_C01f175d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 79 out of 300. ID=191023110002_C01f176\n",
      "blue: MFGTMP_191023110002_C01f176d0.TIF\n",
      "green: MFGTMP_191023110002_C01f176d1.TIF\n",
      "red: MFGTMP_191023110002_C01f176d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 80 out of 300. ID=191023110002_C01f177\n",
      "blue: MFGTMP_191023110002_C01f177d0.TIF\n",
      "green: MFGTMP_191023110002_C01f177d1.TIF\n",
      "red: MFGTMP_191023110002_C01f177d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1899.76\n",
      "Image is sharp! 2nd focus check FM2=373.81\n",
      "Processed image saved as merged_191023110002_C01f177.TIF \n",
      "\n",
      "\n",
      "Processing image set 81 out of 300. ID=191023110002_C01f178\n",
      "blue: MFGTMP_191023110002_C01f178d0.TIF\n",
      "green: MFGTMP_191023110002_C01f178d1.TIF\n",
      "red: MFGTMP_191023110002_C01f178d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1450.48\n",
      "Image is sharp! 2nd focus check FM2=262.56\n",
      "Processed image saved as merged_191023110002_C01f178.TIF \n",
      "\n",
      "\n",
      "Processing image set 82 out of 300. ID=191023110002_C01f179\n",
      "blue: MFGTMP_191023110002_C01f179d0.TIF\n",
      "green: MFGTMP_191023110002_C01f179d1.TIF\n",
      "red: MFGTMP_191023110002_C01f179d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=809.76\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 83 out of 300. ID=191023110002_C01f17\n",
      "blue: MFGTMP_191023110002_C01f170d0.TIF\n",
      "green: MFGTMP_191023110002_C01f170d1.TIF\n",
      "red: MFGTMP_191023110002_C01f170d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f171d0.TIF\n",
      "green: MFGTMP_191023110002_C01f171d1.TIF\n",
      "red: MFGTMP_191023110002_C01f171d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f172d0.TIF\n",
      "green: MFGTMP_191023110002_C01f172d1.TIF\n",
      "red: MFGTMP_191023110002_C01f172d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f173d0.TIF\n",
      "green: MFGTMP_191023110002_C01f173d1.TIF\n",
      "red: MFGTMP_191023110002_C01f173d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f174d0.TIF\n",
      "green: MFGTMP_191023110002_C01f174d1.TIF\n",
      "red: MFGTMP_191023110002_C01f174d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f175d0.TIF\n",
      "green: MFGTMP_191023110002_C01f175d1.TIF\n",
      "red: MFGTMP_191023110002_C01f175d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f176d0.TIF\n",
      "green: MFGTMP_191023110002_C01f176d1.TIF\n",
      "red: MFGTMP_191023110002_C01f176d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f177d0.TIF\n",
      "green: MFGTMP_191023110002_C01f177d1.TIF\n",
      "red: MFGTMP_191023110002_C01f177d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f178d0.TIF\n",
      "green: MFGTMP_191023110002_C01f178d1.TIF\n",
      "red: MFGTMP_191023110002_C01f178d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f179d0.TIF\n",
      "green: MFGTMP_191023110002_C01f179d1.TIF\n",
      "red: MFGTMP_191023110002_C01f179d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f17d0.TIF\n",
      "green: MFGTMP_191023110002_C01f17d1.TIF\n",
      "red: MFGTMP_191023110002_C01f17d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=548.44\n",
      "Image is sharp! 2nd focus check FM2=393.97\n",
      "Processed image saved as merged_191023110002_C01f17.TIF \n",
      "\n",
      "\n",
      "Processing image set 84 out of 300. ID=191023110002_C01f180\n",
      "blue: MFGTMP_191023110002_C01f180d0.TIF\n",
      "green: MFGTMP_191023110002_C01f180d1.TIF\n",
      "red: MFGTMP_191023110002_C01f180d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=609.83\n",
      "Image is sharp! 2nd focus check FM2=520.47\n",
      "Processed image saved as merged_191023110002_C01f180.TIF \n",
      "\n",
      "\n",
      "Processing image set 85 out of 300. ID=191023110002_C01f181\n",
      "blue: MFGTMP_191023110002_C01f181d0.TIF\n",
      "green: MFGTMP_191023110002_C01f181d1.TIF\n",
      "red: MFGTMP_191023110002_C01f181d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=602.02\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 86 out of 300. ID=191023110002_C01f182\n",
      "blue: MFGTMP_191023110002_C01f182d0.TIF\n",
      "green: MFGTMP_191023110002_C01f182d1.TIF\n",
      "red: MFGTMP_191023110002_C01f182d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 87 out of 300. ID=191023110002_C01f183\n",
      "blue: MFGTMP_191023110002_C01f183d0.TIF\n",
      "green: MFGTMP_191023110002_C01f183d1.TIF\n",
      "red: MFGTMP_191023110002_C01f183d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 88 out of 300. ID=191023110002_C01f184\n",
      "blue: MFGTMP_191023110002_C01f184d0.TIF\n",
      "green: MFGTMP_191023110002_C01f184d1.TIF\n",
      "red: MFGTMP_191023110002_C01f184d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 89 out of 300. ID=191023110002_C01f185\n",
      "blue: MFGTMP_191023110002_C01f185d0.TIF\n"
     ]
    },
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "green: MFGTMP_191023110002_C01f185d1.TIF\n",
      "red: MFGTMP_191023110002_C01f185d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=982.71\n",
      "Image is sharp! 2nd focus check FM2=264.67\n",
      "Processed image saved as merged_191023110002_C01f185.TIF \n",
      "\n",
      "\n",
      "Processing image set 90 out of 300. ID=191023110002_C01f186\n",
      "blue: MFGTMP_191023110002_C01f186d0.TIF\n",
      "green: MFGTMP_191023110002_C01f186d1.TIF\n",
      "red: MFGTMP_191023110002_C01f186d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=557.87\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 91 out of 300. ID=191023110002_C01f187\n",
      "blue: MFGTMP_191023110002_C01f187d0.TIF\n",
      "green: MFGTMP_191023110002_C01f187d1.TIF\n",
      "red: MFGTMP_191023110002_C01f187d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1553.05\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 92 out of 300. ID=191023110002_C01f188\n",
      "blue: MFGTMP_191023110002_C01f188d0.TIF\n",
      "green: MFGTMP_191023110002_C01f188d1.TIF\n",
      "red: MFGTMP_191023110002_C01f188d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 93 out of 300. ID=191023110002_C01f189\n",
      "blue: MFGTMP_191023110002_C01f189d0.TIF\n",
      "green: MFGTMP_191023110002_C01f189d1.TIF\n",
      "red: MFGTMP_191023110002_C01f189d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1363.41\n",
      "Image is sharp! 2nd focus check FM2=1054.71\n",
      "Processed image saved as merged_191023110002_C01f189.TIF \n",
      "\n",
      "\n",
      "Processing image set 94 out of 300. ID=191023110002_C01f18\n",
      "blue: MFGTMP_191023110002_C01f180d0.TIF\n",
      "green: MFGTMP_191023110002_C01f180d1.TIF\n",
      "red: MFGTMP_191023110002_C01f180d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f181d0.TIF\n",
      "green: MFGTMP_191023110002_C01f181d1.TIF\n",
      "red: MFGTMP_191023110002_C01f181d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f182d0.TIF\n",
      "green: MFGTMP_191023110002_C01f182d1.TIF\n",
      "red: MFGTMP_191023110002_C01f182d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f183d0.TIF\n",
      "green: MFGTMP_191023110002_C01f183d1.TIF\n",
      "red: MFGTMP_191023110002_C01f183d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f184d0.TIF\n",
      "green: MFGTMP_191023110002_C01f184d1.TIF\n",
      "red: MFGTMP_191023110002_C01f184d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f185d0.TIF\n",
      "green: MFGTMP_191023110002_C01f185d1.TIF\n",
      "red: MFGTMP_191023110002_C01f185d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f186d0.TIF\n",
      "green: MFGTMP_191023110002_C01f186d1.TIF\n",
      "red: MFGTMP_191023110002_C01f186d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f187d0.TIF\n",
      "green: MFGTMP_191023110002_C01f187d1.TIF\n",
      "red: MFGTMP_191023110002_C01f187d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f188d0.TIF\n",
      "green: MFGTMP_191023110002_C01f188d1.TIF\n",
      "red: MFGTMP_191023110002_C01f188d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f189d0.TIF\n",
      "green: MFGTMP_191023110002_C01f189d1.TIF\n",
      "red: MFGTMP_191023110002_C01f189d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f18d0.TIF\n",
      "green: MFGTMP_191023110002_C01f18d1.TIF\n",
      "red: MFGTMP_191023110002_C01f18d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=883.00\n",
      "Image is sharp! 2nd focus check FM2=226.22\n",
      "Processed image saved as merged_191023110002_C01f18.TIF \n",
      "\n",
      "\n",
      "Processing image set 95 out of 300. ID=191023110002_C01f190\n",
      "blue: MFGTMP_191023110002_C01f190d0.TIF\n",
      "green: MFGTMP_191023110002_C01f190d1.TIF\n",
      "red: MFGTMP_191023110002_C01f190d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1540.58\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 96 out of 300. ID=191023110002_C01f191\n",
      "blue: MFGTMP_191023110002_C01f191d0.TIF\n",
      "green: MFGTMP_191023110002_C01f191d1.TIF\n",
      "red: MFGTMP_191023110002_C01f191d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 97 out of 300. ID=191023110002_C01f192\n",
      "blue: MFGTMP_191023110002_C01f192d0.TIF\n",
      "green: MFGTMP_191023110002_C01f192d1.TIF\n",
      "red: MFGTMP_191023110002_C01f192d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1570.78\n",
      "Image is sharp! 2nd focus check FM2=245.69\n",
      "Processed image saved as merged_191023110002_C01f192.TIF \n",
      "\n",
      "\n",
      "Processing image set 98 out of 300. ID=191023110002_C01f193\n",
      "blue: MFGTMP_191023110002_C01f193d0.TIF\n",
      "green: MFGTMP_191023110002_C01f193d1.TIF\n",
      "red: MFGTMP_191023110002_C01f193d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1582.50\n",
      "Image is sharp! 2nd focus check FM2=391.30\n",
      "Processed image saved as merged_191023110002_C01f193.TIF \n",
      "\n",
      "\n",
      "Processing image set 99 out of 300. ID=191023110002_C01f194\n",
      "blue: MFGTMP_191023110002_C01f194d0.TIF\n",
      "green: MFGTMP_191023110002_C01f194d1.TIF\n",
      "red: MFGTMP_191023110002_C01f194d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 100 out of 300. ID=191023110002_C01f195\n",
      "blue: MFGTMP_191023110002_C01f195d0.TIF\n",
      "green: MFGTMP_191023110002_C01f195d1.TIF\n",
      "red: MFGTMP_191023110002_C01f195d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1601.35\n",
      "Image is sharp! 2nd focus check FM2=225.60\n",
      "Processed image saved as merged_191023110002_C01f195.TIF \n",
      "\n",
      "\n",
      "Processing image set 101 out of 300. ID=191023110002_C01f196\n",
      "blue: MFGTMP_191023110002_C01f196d0.TIF\n",
      "green: MFGTMP_191023110002_C01f196d1.TIF\n",
      "red: MFGTMP_191023110002_C01f196d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 102 out of 300. ID=191023110002_C01f197\n",
      "blue: MFGTMP_191023110002_C01f197d0.TIF\n",
      "green: MFGTMP_191023110002_C01f197d1.TIF\n",
      "red: MFGTMP_191023110002_C01f197d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 103 out of 300. ID=191023110002_C01f198\n",
      "blue: MFGTMP_191023110002_C01f198d0.TIF\n",
      "green: MFGTMP_191023110002_C01f198d1.TIF\n",
      "red: MFGTMP_191023110002_C01f198d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 104 out of 300. ID=191023110002_C01f199\n",
      "blue: MFGTMP_191023110002_C01f199d0.TIF\n",
      "green: MFGTMP_191023110002_C01f199d1.TIF\n",
      "red: MFGTMP_191023110002_C01f199d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=592.97\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 105 out of 300. ID=191023110002_C01f19\n",
      "blue: MFGTMP_191023110002_C01f190d0.TIF\n",
      "green: MFGTMP_191023110002_C01f190d1.TIF\n",
      "red: MFGTMP_191023110002_C01f190d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f191d0.TIF\n",
      "green: MFGTMP_191023110002_C01f191d1.TIF\n",
      "red: MFGTMP_191023110002_C01f191d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f192d0.TIF\n",
      "green: MFGTMP_191023110002_C01f192d1.TIF\n",
      "red: MFGTMP_191023110002_C01f192d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f193d0.TIF\n",
      "green: MFGTMP_191023110002_C01f193d1.TIF\n",
      "red: MFGTMP_191023110002_C01f193d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f194d0.TIF\n",
      "green: MFGTMP_191023110002_C01f194d1.TIF\n",
      "red: MFGTMP_191023110002_C01f194d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f195d0.TIF\n",
      "green: MFGTMP_191023110002_C01f195d1.TIF\n",
      "red: MFGTMP_191023110002_C01f195d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f196d0.TIF\n",
      "green: MFGTMP_191023110002_C01f196d1.TIF\n",
      "red: MFGTMP_191023110002_C01f196d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f197d0.TIF\n",
      "green: MFGTMP_191023110002_C01f197d1.TIF\n",
      "red: MFGTMP_191023110002_C01f197d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f198d0.TIF\n",
      "green: MFGTMP_191023110002_C01f198d1.TIF\n",
      "red: MFGTMP_191023110002_C01f198d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f199d0.TIF\n",
      "green: MFGTMP_191023110002_C01f199d1.TIF\n",
      "red: MFGTMP_191023110002_C01f199d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f19d0.TIF\n",
      "green: MFGTMP_191023110002_C01f19d1.TIF\n",
      "red: MFGTMP_191023110002_C01f19d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 106 out of 300. ID=191023110002_C01f200\n",
      "blue: MFGTMP_191023110002_C01f200d0.TIF\n",
      "green: MFGTMP_191023110002_C01f200d1.TIF\n",
      "red: MFGTMP_191023110002_C01f200d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1261.34\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 107 out of 300. ID=191023110002_C01f201\n",
      "blue: MFGTMP_191023110002_C01f201d0.TIF\n",
      "green: MFGTMP_191023110002_C01f201d1.TIF\n",
      "red: MFGTMP_191023110002_C01f201d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1029.22\n",
      "Image is sharp! 2nd focus check FM2=415.98\n",
      "Processed image saved as merged_191023110002_C01f201.TIF \n",
      "\n",
      "\n",
      "Processing image set 108 out of 300. ID=191023110002_C01f202\n",
      "blue: MFGTMP_191023110002_C01f202d0.TIF\n",
      "green: MFGTMP_191023110002_C01f202d1.TIF\n",
      "red: MFGTMP_191023110002_C01f202d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1671.68\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 109 out of 300. ID=191023110002_C01f203\n",
      "blue: MFGTMP_191023110002_C01f203d0.TIF\n",
      "green: MFGTMP_191023110002_C01f203d1.TIF\n",
      "red: MFGTMP_191023110002_C01f203d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1073.94\n",
      "Image is sharp! 2nd focus check FM2=304.23\n",
      "Processed image saved as merged_191023110002_C01f203.TIF \n",
      "\n",
      "\n",
      "Processing image set 110 out of 300. ID=191023110002_C01f204\n",
      "blue: MFGTMP_191023110002_C01f204d0.TIF\n",
      "green: MFGTMP_191023110002_C01f204d1.TIF\n",
      "red: MFGTMP_191023110002_C01f204d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 111 out of 300. ID=191023110002_C01f205\n",
      "blue: MFGTMP_191023110002_C01f205d0.TIF\n",
      "green: MFGTMP_191023110002_C01f205d1.TIF\n",
      "red: MFGTMP_191023110002_C01f205d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 112 out of 300. ID=191023110002_C01f206\n",
      "blue: MFGTMP_191023110002_C01f206d0.TIF\n",
      "green: MFGTMP_191023110002_C01f206d1.TIF\n"
     ]
    },
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "red: MFGTMP_191023110002_C01f206d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 113 out of 300. ID=191023110002_C01f207\n",
      "blue: MFGTMP_191023110002_C01f207d0.TIF\n",
      "green: MFGTMP_191023110002_C01f207d1.TIF\n",
      "red: MFGTMP_191023110002_C01f207d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 114 out of 300. ID=191023110002_C01f208\n",
      "blue: MFGTMP_191023110002_C01f208d0.TIF\n",
      "green: MFGTMP_191023110002_C01f208d1.TIF\n",
      "red: MFGTMP_191023110002_C01f208d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 115 out of 300. ID=191023110002_C01f209\n",
      "blue: MFGTMP_191023110002_C01f209d0.TIF\n",
      "green: MFGTMP_191023110002_C01f209d1.TIF\n",
      "red: MFGTMP_191023110002_C01f209d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 116 out of 300. ID=191023110002_C01f20\n",
      "blue: MFGTMP_191023110002_C01f200d0.TIF\n",
      "green: MFGTMP_191023110002_C01f200d1.TIF\n",
      "red: MFGTMP_191023110002_C01f200d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f201d0.TIF\n",
      "green: MFGTMP_191023110002_C01f201d1.TIF\n",
      "red: MFGTMP_191023110002_C01f201d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f202d0.TIF\n",
      "green: MFGTMP_191023110002_C01f202d1.TIF\n",
      "red: MFGTMP_191023110002_C01f202d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f203d0.TIF\n",
      "green: MFGTMP_191023110002_C01f203d1.TIF\n",
      "red: MFGTMP_191023110002_C01f203d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f204d0.TIF\n",
      "green: MFGTMP_191023110002_C01f204d1.TIF\n",
      "red: MFGTMP_191023110002_C01f204d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f205d0.TIF\n",
      "green: MFGTMP_191023110002_C01f205d1.TIF\n",
      "red: MFGTMP_191023110002_C01f205d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f206d0.TIF\n",
      "green: MFGTMP_191023110002_C01f206d1.TIF\n",
      "red: MFGTMP_191023110002_C01f206d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f207d0.TIF\n",
      "green: MFGTMP_191023110002_C01f207d1.TIF\n",
      "red: MFGTMP_191023110002_C01f207d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f208d0.TIF\n",
      "green: MFGTMP_191023110002_C01f208d1.TIF\n",
      "red: MFGTMP_191023110002_C01f208d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f209d0.TIF\n",
      "green: MFGTMP_191023110002_C01f209d1.TIF\n",
      "red: MFGTMP_191023110002_C01f209d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f20d0.TIF\n",
      "green: MFGTMP_191023110002_C01f20d1.TIF\n",
      "red: MFGTMP_191023110002_C01f20d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 117 out of 300. ID=191023110002_C01f210\n",
      "blue: MFGTMP_191023110002_C01f210d0.TIF\n",
      "green: MFGTMP_191023110002_C01f210d1.TIF\n",
      "red: MFGTMP_191023110002_C01f210d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 118 out of 300. ID=191023110002_C01f211\n",
      "blue: MFGTMP_191023110002_C01f211d0.TIF\n",
      "green: MFGTMP_191023110002_C01f211d1.TIF\n",
      "red: MFGTMP_191023110002_C01f211d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1462.94\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 119 out of 300. ID=191023110002_C01f212\n",
      "blue: MFGTMP_191023110002_C01f212d0.TIF\n",
      "green: MFGTMP_191023110002_C01f212d1.TIF\n",
      "red: MFGTMP_191023110002_C01f212d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=3046.31\n",
      "Image is sharp! 2nd focus check FM2=309.07\n",
      "Processed image saved as merged_191023110002_C01f212.TIF \n",
      "\n",
      "\n",
      "Processing image set 120 out of 300. ID=191023110002_C01f213\n",
      "blue: MFGTMP_191023110002_C01f213d0.TIF\n",
      "green: MFGTMP_191023110002_C01f213d1.TIF\n",
      "red: MFGTMP_191023110002_C01f213d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1348.53\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 121 out of 300. ID=191023110002_C01f214\n",
      "blue: MFGTMP_191023110002_C01f214d0.TIF\n",
      "green: MFGTMP_191023110002_C01f214d1.TIF\n",
      "red: MFGTMP_191023110002_C01f214d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=682.57\n",
      "Image is sharp! 2nd focus check FM2=268.02\n",
      "Processed image saved as merged_191023110002_C01f214.TIF \n",
      "\n",
      "\n",
      "Processing image set 122 out of 300. ID=191023110002_C01f21\n",
      "blue: MFGTMP_191023110002_C01f210d0.TIF\n",
      "green: MFGTMP_191023110002_C01f210d1.TIF\n",
      "red: MFGTMP_191023110002_C01f210d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f211d0.TIF\n",
      "green: MFGTMP_191023110002_C01f211d1.TIF\n",
      "red: MFGTMP_191023110002_C01f211d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f212d0.TIF\n",
      "green: MFGTMP_191023110002_C01f212d1.TIF\n",
      "red: MFGTMP_191023110002_C01f212d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f213d0.TIF\n",
      "green: MFGTMP_191023110002_C01f213d1.TIF\n",
      "red: MFGTMP_191023110002_C01f213d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f214d0.TIF\n",
      "green: MFGTMP_191023110002_C01f214d1.TIF\n",
      "red: MFGTMP_191023110002_C01f214d2.TIF\n",
      "blue: MFGTMP_191023110002_C01f21d0.TIF\n",
      "green: MFGTMP_191023110002_C01f21d1.TIF\n",
      "red: MFGTMP_191023110002_C01f21d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=566.98\n",
      "Image is sharp! 2nd focus check FM2=454.43\n",
      "Processed image saved as merged_191023110002_C01f21.TIF \n",
      "\n",
      "\n",
      "Processing image set 123 out of 300. ID=191023110002_C01f22\n",
      "blue: MFGTMP_191023110002_C01f22d0.TIF\n",
      "green: MFGTMP_191023110002_C01f22d1.TIF\n",
      "red: MFGTMP_191023110002_C01f22d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1521.98\n",
      "Image is sharp! 2nd focus check FM2=559.85\n",
      "Processed image saved as merged_191023110002_C01f22.TIF \n",
      "\n",
      "\n",
      "Processing image set 124 out of 300. ID=191023110002_C01f23\n",
      "blue: MFGTMP_191023110002_C01f23d0.TIF\n",
      "green: MFGTMP_191023110002_C01f23d1.TIF\n",
      "red: MFGTMP_191023110002_C01f23d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 125 out of 300. ID=191023110002_C01f24\n",
      "blue: MFGTMP_191023110002_C01f24d0.TIF\n",
      "green: MFGTMP_191023110002_C01f24d1.TIF\n",
      "red: MFGTMP_191023110002_C01f24d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 126 out of 300. ID=191023110002_C01f25\n",
      "blue: MFGTMP_191023110002_C01f25d0.TIF\n",
      "green: MFGTMP_191023110002_C01f25d1.TIF\n",
      "red: MFGTMP_191023110002_C01f25d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 127 out of 300. ID=191023110002_C01f26\n",
      "blue: MFGTMP_191023110002_C01f26d0.TIF\n",
      "green: MFGTMP_191023110002_C01f26d1.TIF\n",
      "red: MFGTMP_191023110002_C01f26d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 128 out of 300. ID=191023110002_C01f27\n",
      "blue: MFGTMP_191023110002_C01f27d0.TIF\n",
      "green: MFGTMP_191023110002_C01f27d1.TIF\n",
      "red: MFGTMP_191023110002_C01f27d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 129 out of 300. ID=191023110002_C01f28\n",
      "blue: MFGTMP_191023110002_C01f28d0.TIF\n",
      "green: MFGTMP_191023110002_C01f28d1.TIF\n",
      "red: MFGTMP_191023110002_C01f28d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 130 out of 300. ID=191023110002_C01f29\n",
      "blue: MFGTMP_191023110002_C01f29d0.TIF\n",
      "green: MFGTMP_191023110002_C01f29d1.TIF\n",
      "red: MFGTMP_191023110002_C01f29d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 131 out of 300. ID=191023110002_C01f30\n",
      "blue: MFGTMP_191023110002_C01f30d0.TIF\n",
      "green: MFGTMP_191023110002_C01f30d1.TIF\n",
      "red: MFGTMP_191023110002_C01f30d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 132 out of 300. ID=191023110002_C01f31\n",
      "blue: MFGTMP_191023110002_C01f31d0.TIF\n",
      "green: MFGTMP_191023110002_C01f31d1.TIF\n",
      "red: MFGTMP_191023110002_C01f31d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 133 out of 300. ID=191023110002_C01f32\n",
      "blue: MFGTMP_191023110002_C01f32d0.TIF\n",
      "green: MFGTMP_191023110002_C01f32d1.TIF\n",
      "red: MFGTMP_191023110002_C01f32d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 134 out of 300. ID=191023110002_C01f33\n",
      "blue: MFGTMP_191023110002_C01f33d0.TIF\n",
      "green: MFGTMP_191023110002_C01f33d1.TIF\n",
      "red: MFGTMP_191023110002_C01f33d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 135 out of 300. ID=191023110002_C01f34\n",
      "blue: MFGTMP_191023110002_C01f34d0.TIF\n",
      "green: MFGTMP_191023110002_C01f34d1.TIF\n",
      "red: MFGTMP_191023110002_C01f34d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1104.32\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 136 out of 300. ID=191023110002_C01f35\n",
      "blue: MFGTMP_191023110002_C01f35d0.TIF\n",
      "green: MFGTMP_191023110002_C01f35d1.TIF\n",
      "red: MFGTMP_191023110002_C01f35d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=866.88\n",
      "Image is sharp! 2nd focus check FM2=212.52\n",
      "Processed image saved as merged_191023110002_C01f35.TIF \n",
      "\n",
      "\n",
      "Processing image set 137 out of 300. ID=191023110002_C01f36\n",
      "blue: MFGTMP_191023110002_C01f36d0.TIF\n",
      "green: MFGTMP_191023110002_C01f36d1.TIF\n",
      "red: MFGTMP_191023110002_C01f36d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1437.52\n",
      "Image is sharp! 2nd focus check FM2=562.83\n",
      "Processed image saved as merged_191023110002_C01f36.TIF \n",
      "\n",
      "\n",
      "Processing image set 138 out of 300. ID=191023110002_C01f37\n",
      "blue: MFGTMP_191023110002_C01f37d0.TIF\n",
      "green: MFGTMP_191023110002_C01f37d1.TIF\n",
      "red: MFGTMP_191023110002_C01f37d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=778.69\n"
     ]
    },
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 139 out of 300. ID=191023110002_C01f38\n",
      "blue: MFGTMP_191023110002_C01f38d0.TIF\n",
      "green: MFGTMP_191023110002_C01f38d1.TIF\n",
      "red: MFGTMP_191023110002_C01f38d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1164.85\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 140 out of 300. ID=191023110002_C01f39\n",
      "blue: MFGTMP_191023110002_C01f39d0.TIF\n",
      "green: MFGTMP_191023110002_C01f39d1.TIF\n",
      "red: MFGTMP_191023110002_C01f39d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 141 out of 300. ID=191023110002_C01f40\n",
      "blue: MFGTMP_191023110002_C01f40d0.TIF\n",
      "green: MFGTMP_191023110002_C01f40d1.TIF\n",
      "red: MFGTMP_191023110002_C01f40d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 142 out of 300. ID=191023110002_C01f41\n",
      "blue: MFGTMP_191023110002_C01f41d0.TIF\n",
      "green: MFGTMP_191023110002_C01f41d1.TIF\n",
      "red: MFGTMP_191023110002_C01f41d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 143 out of 300. ID=191023110002_C01f42\n",
      "blue: MFGTMP_191023110002_C01f42d0.TIF\n",
      "green: MFGTMP_191023110002_C01f42d1.TIF\n",
      "red: MFGTMP_191023110002_C01f42d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 144 out of 300. ID=191023110002_C01f43\n",
      "blue: MFGTMP_191023110002_C01f43d0.TIF\n",
      "green: MFGTMP_191023110002_C01f43d1.TIF\n",
      "red: MFGTMP_191023110002_C01f43d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 145 out of 300. ID=191023110002_C01f44\n",
      "blue: MFGTMP_191023110002_C01f44d0.TIF\n",
      "green: MFGTMP_191023110002_C01f44d1.TIF\n",
      "red: MFGTMP_191023110002_C01f44d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 146 out of 300. ID=191023110002_C01f45\n",
      "blue: MFGTMP_191023110002_C01f45d0.TIF\n",
      "green: MFGTMP_191023110002_C01f45d1.TIF\n",
      "red: MFGTMP_191023110002_C01f45d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 147 out of 300. ID=191023110002_C01f46\n",
      "blue: MFGTMP_191023110002_C01f46d0.TIF\n",
      "green: MFGTMP_191023110002_C01f46d1.TIF\n",
      "red: MFGTMP_191023110002_C01f46d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 148 out of 300. ID=191023110002_C01f47\n",
      "blue: MFGTMP_191023110002_C01f47d0.TIF\n",
      "green: MFGTMP_191023110002_C01f47d1.TIF\n",
      "red: MFGTMP_191023110002_C01f47d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 149 out of 300. ID=191023110002_C01f48\n",
      "blue: MFGTMP_191023110002_C01f48d0.TIF\n",
      "green: MFGTMP_191023110002_C01f48d1.TIF\n",
      "red: MFGTMP_191023110002_C01f48d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 150 out of 300. ID=191023110002_C01f49\n",
      "blue: MFGTMP_191023110002_C01f49d0.TIF\n",
      "green: MFGTMP_191023110002_C01f49d1.TIF\n",
      "red: MFGTMP_191023110002_C01f49d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 151 out of 300. ID=191023110002_C01f50\n",
      "blue: MFGTMP_191023110002_C01f50d0.TIF\n",
      "green: MFGTMP_191023110002_C01f50d1.TIF\n",
      "red: MFGTMP_191023110002_C01f50d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 152 out of 300. ID=191023110002_C01f51\n",
      "blue: MFGTMP_191023110002_C01f51d0.TIF\n",
      "green: MFGTMP_191023110002_C01f51d1.TIF\n",
      "red: MFGTMP_191023110002_C01f51d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1504.55\n",
      "Image is sharp! 2nd focus check FM2=544.47\n",
      "Processed image saved as merged_191023110002_C01f51.TIF \n",
      "\n",
      "\n",
      "Processing image set 153 out of 300. ID=191023110002_C01f52\n",
      "blue: MFGTMP_191023110002_C01f52d0.TIF\n",
      "green: MFGTMP_191023110002_C01f52d1.TIF\n",
      "red: MFGTMP_191023110002_C01f52d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 154 out of 300. ID=191023110002_C01f53\n",
      "blue: MFGTMP_191023110002_C01f53d0.TIF\n",
      "green: MFGTMP_191023110002_C01f53d1.TIF\n",
      "red: MFGTMP_191023110002_C01f53d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 155 out of 300. ID=191023110002_C01f54\n",
      "blue: MFGTMP_191023110002_C01f54d0.TIF\n",
      "green: MFGTMP_191023110002_C01f54d1.TIF\n",
      "red: MFGTMP_191023110002_C01f54d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=916.05\n",
      "Image is sharp! 2nd focus check FM2=473.28\n",
      "Processed image saved as merged_191023110002_C01f54.TIF \n",
      "\n",
      "\n",
      "Processing image set 156 out of 300. ID=191023110002_C01f55\n",
      "blue: MFGTMP_191023110002_C01f55d0.TIF\n",
      "green: MFGTMP_191023110002_C01f55d1.TIF\n",
      "red: MFGTMP_191023110002_C01f55d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=736.03\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 157 out of 300. ID=191023110002_C01f56\n",
      "blue: MFGTMP_191023110002_C01f56d0.TIF\n",
      "green: MFGTMP_191023110002_C01f56d1.TIF\n",
      "red: MFGTMP_191023110002_C01f56d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=691.19\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 158 out of 300. ID=191023110002_C01f57\n",
      "blue: MFGTMP_191023110002_C01f57d0.TIF\n",
      "green: MFGTMP_191023110002_C01f57d1.TIF\n",
      "red: MFGTMP_191023110002_C01f57d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 159 out of 300. ID=191023110002_C01f58\n",
      "blue: MFGTMP_191023110002_C01f58d0.TIF\n",
      "green: MFGTMP_191023110002_C01f58d1.TIF\n",
      "red: MFGTMP_191023110002_C01f58d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 160 out of 300. ID=191023110002_C01f59\n",
      "blue: MFGTMP_191023110002_C01f59d0.TIF\n",
      "green: MFGTMP_191023110002_C01f59d1.TIF\n",
      "red: MFGTMP_191023110002_C01f59d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 161 out of 300. ID=191023110002_C01f60\n",
      "blue: MFGTMP_191023110002_C01f60d0.TIF\n",
      "green: MFGTMP_191023110002_C01f60d1.TIF\n",
      "red: MFGTMP_191023110002_C01f60d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 162 out of 300. ID=191023110002_C01f61\n",
      "blue: MFGTMP_191023110002_C01f61d0.TIF\n",
      "green: MFGTMP_191023110002_C01f61d1.TIF\n",
      "red: MFGTMP_191023110002_C01f61d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 163 out of 300. ID=191023110002_C01f62\n",
      "blue: MFGTMP_191023110002_C01f62d0.TIF\n",
      "green: MFGTMP_191023110002_C01f62d1.TIF\n",
      "red: MFGTMP_191023110002_C01f62d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1155.85\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 164 out of 300. ID=191023110002_C01f63\n",
      "blue: MFGTMP_191023110002_C01f63d0.TIF\n",
      "green: MFGTMP_191023110002_C01f63d1.TIF\n",
      "red: MFGTMP_191023110002_C01f63d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1091.36\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 165 out of 300. ID=191023110002_C01f64\n",
      "blue: MFGTMP_191023110002_C01f64d0.TIF\n",
      "green: MFGTMP_191023110002_C01f64d1.TIF\n",
      "red: MFGTMP_191023110002_C01f64d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1700.08\n",
      "Image is sharp! 2nd focus check FM2=330.40\n",
      "Processed image saved as merged_191023110002_C01f64.TIF \n",
      "\n",
      "\n",
      "Processing image set 166 out of 300. ID=191023110002_C01f65\n",
      "blue: MFGTMP_191023110002_C01f65d0.TIF\n",
      "green: MFGTMP_191023110002_C01f65d1.TIF\n",
      "red: MFGTMP_191023110002_C01f65d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 167 out of 300. ID=191023110002_C01f66\n",
      "blue: MFGTMP_191023110002_C01f66d0.TIF\n",
      "green: MFGTMP_191023110002_C01f66d1.TIF\n",
      "red: MFGTMP_191023110002_C01f66d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 168 out of 300. ID=191023110002_C01f67\n",
      "blue: MFGTMP_191023110002_C01f67d0.TIF\n",
      "green: MFGTMP_191023110002_C01f67d1.TIF\n",
      "red: MFGTMP_191023110002_C01f67d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 169 out of 300. ID=191023110002_C01f68\n",
      "blue: MFGTMP_191023110002_C01f68d0.TIF\n",
      "green: MFGTMP_191023110002_C01f68d1.TIF\n",
      "red: MFGTMP_191023110002_C01f68d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=556.81\n",
      "Image is sharp! 2nd focus check FM2=320.05\n",
      "Processed image saved as merged_191023110002_C01f68.TIF \n",
      "\n",
      "\n",
      "Processing image set 170 out of 300. ID=191023110002_C01f69\n",
      "blue: MFGTMP_191023110002_C01f69d0.TIF\n",
      "green: MFGTMP_191023110002_C01f69d1.TIF\n",
      "red: MFGTMP_191023110002_C01f69d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1547.15\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 171 out of 300. ID=191023110002_C01f70\n",
      "blue: MFGTMP_191023110002_C01f70d0.TIF\n",
      "green: MFGTMP_191023110002_C01f70d1.TIF\n",
      "red: MFGTMP_191023110002_C01f70d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=623.10\n",
      "Image is sharp! 2nd focus check FM2=542.80\n",
      "Processed image saved as merged_191023110002_C01f70.TIF \n",
      "\n",
      "\n",
      "Processing image set 172 out of 300. ID=191023110002_C01f71\n",
      "blue: MFGTMP_191023110002_C01f71d0.TIF\n",
      "green: MFGTMP_191023110002_C01f71d1.TIF\n",
      "red: MFGTMP_191023110002_C01f71d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 173 out of 300. ID=191023110002_C01f72\n",
      "blue: MFGTMP_191023110002_C01f72d0.TIF\n",
      "green: MFGTMP_191023110002_C01f72d1.TIF\n",
      "red: MFGTMP_191023110002_C01f72d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 174 out of 300. ID=191023110002_C01f73\n",
      "blue: MFGTMP_191023110002_C01f73d0.TIF\n",
      "green: MFGTMP_191023110002_C01f73d1.TIF\n",
      "red: MFGTMP_191023110002_C01f73d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 175 out of 300. ID=191023110002_C01f74\n",
      "blue: MFGTMP_191023110002_C01f74d0.TIF\n",
      "green: MFGTMP_191023110002_C01f74d1.TIF\n",
      "red: MFGTMP_191023110002_C01f74d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1631.74\n"
     ]
    },
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "Image is sharp! 2nd focus check FM2=695.16\n",
      "Processed image saved as merged_191023110002_C01f74.TIF \n",
      "\n",
      "\n",
      "Processing image set 176 out of 300. ID=191023110002_C01f75\n",
      "blue: MFGTMP_191023110002_C01f75d0.TIF\n",
      "green: MFGTMP_191023110002_C01f75d1.TIF\n",
      "red: MFGTMP_191023110002_C01f75d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 177 out of 300. ID=191023110002_C01f76\n",
      "blue: MFGTMP_191023110002_C01f76d0.TIF\n",
      "green: MFGTMP_191023110002_C01f76d1.TIF\n",
      "red: MFGTMP_191023110002_C01f76d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 178 out of 300. ID=191023110002_C01f77\n",
      "blue: MFGTMP_191023110002_C01f77d0.TIF\n",
      "green: MFGTMP_191023110002_C01f77d1.TIF\n",
      "red: MFGTMP_191023110002_C01f77d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 179 out of 300. ID=191023110002_C01f78\n",
      "blue: MFGTMP_191023110002_C01f78d0.TIF\n",
      "green: MFGTMP_191023110002_C01f78d1.TIF\n",
      "red: MFGTMP_191023110002_C01f78d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 180 out of 300. ID=191023110002_C01f79\n",
      "blue: MFGTMP_191023110002_C01f79d0.TIF\n",
      "green: MFGTMP_191023110002_C01f79d1.TIF\n",
      "red: MFGTMP_191023110002_C01f79d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 181 out of 300. ID=191023110002_C01f80\n",
      "blue: MFGTMP_191023110002_C01f80d0.TIF\n",
      "green: MFGTMP_191023110002_C01f80d1.TIF\n",
      "red: MFGTMP_191023110002_C01f80d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 182 out of 300. ID=191023110002_C01f81\n",
      "blue: MFGTMP_191023110002_C01f81d0.TIF\n",
      "green: MFGTMP_191023110002_C01f81d1.TIF\n",
      "red: MFGTMP_191023110002_C01f81d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 183 out of 300. ID=191023110002_C01f82\n",
      "blue: MFGTMP_191023110002_C01f82d0.TIF\n",
      "green: MFGTMP_191023110002_C01f82d1.TIF\n",
      "red: MFGTMP_191023110002_C01f82d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 184 out of 300. ID=191023110002_C01f83\n",
      "blue: MFGTMP_191023110002_C01f83d0.TIF\n",
      "green: MFGTMP_191023110002_C01f83d1.TIF\n",
      "red: MFGTMP_191023110002_C01f83d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 185 out of 300. ID=191023110002_C01f84\n",
      "blue: MFGTMP_191023110002_C01f84d0.TIF\n",
      "green: MFGTMP_191023110002_C01f84d1.TIF\n",
      "red: MFGTMP_191023110002_C01f84d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 186 out of 300. ID=191023110002_C01f85\n",
      "blue: MFGTMP_191023110002_C01f85d0.TIF\n",
      "green: MFGTMP_191023110002_C01f85d1.TIF\n",
      "red: MFGTMP_191023110002_C01f85d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1144.01\n",
      "Image is sharp! 2nd focus check FM2=301.01\n",
      "Processed image saved as merged_191023110002_C01f85.TIF \n",
      "\n",
      "\n",
      "Processing image set 187 out of 300. ID=191023110002_C01f86\n",
      "blue: MFGTMP_191023110002_C01f86d0.TIF\n",
      "green: MFGTMP_191023110002_C01f86d1.TIF\n",
      "red: MFGTMP_191023110002_C01f86d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=986.25\n",
      "Image is sharp! 2nd focus check FM2=483.26\n",
      "Processed image saved as merged_191023110002_C01f86.TIF \n",
      "\n",
      "\n",
      "Processing image set 188 out of 300. ID=191023110002_C01f87\n",
      "blue: MFGTMP_191023110002_C01f87d0.TIF\n",
      "green: MFGTMP_191023110002_C01f87d1.TIF\n",
      "red: MFGTMP_191023110002_C01f87d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 189 out of 300. ID=191023110002_C01f88\n",
      "blue: MFGTMP_191023110002_C01f88d0.TIF\n",
      "green: MFGTMP_191023110002_C01f88d1.TIF\n",
      "red: MFGTMP_191023110002_C01f88d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 190 out of 300. ID=191023110002_C01f89\n",
      "blue: MFGTMP_191023110002_C01f89d0.TIF\n",
      "green: MFGTMP_191023110002_C01f89d1.TIF\n",
      "red: MFGTMP_191023110002_C01f89d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=2286.66\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 191 out of 300. ID=191023110002_C01f90\n",
      "blue: MFGTMP_191023110002_C01f90d0.TIF\n",
      "green: MFGTMP_191023110002_C01f90d1.TIF\n",
      "red: MFGTMP_191023110002_C01f90d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1713.29\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 192 out of 300. ID=191023110002_C01f91\n",
      "blue: MFGTMP_191023110002_C01f91d0.TIF\n",
      "green: MFGTMP_191023110002_C01f91d1.TIF\n",
      "red: MFGTMP_191023110002_C01f91d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=925.23\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 193 out of 300. ID=191023110002_C01f92\n",
      "blue: MFGTMP_191023110002_C01f92d0.TIF\n",
      "green: MFGTMP_191023110002_C01f92d1.TIF\n",
      "red: MFGTMP_191023110002_C01f92d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1621.45\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 194 out of 300. ID=191023110002_C01f93\n",
      "blue: MFGTMP_191023110002_C01f93d0.TIF\n",
      "green: MFGTMP_191023110002_C01f93d1.TIF\n",
      "red: MFGTMP_191023110002_C01f93d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=798.41\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 195 out of 300. ID=191023110002_C01f94\n",
      "blue: MFGTMP_191023110002_C01f94d0.TIF\n",
      "green: MFGTMP_191023110002_C01f94d1.TIF\n",
      "red: MFGTMP_191023110002_C01f94d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 196 out of 300. ID=191023110002_C01f95\n",
      "blue: MFGTMP_191023110002_C01f95d0.TIF\n",
      "green: MFGTMP_191023110002_C01f95d1.TIF\n",
      "red: MFGTMP_191023110002_C01f95d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 197 out of 300. ID=191023110002_C01f96\n",
      "blue: MFGTMP_191023110002_C01f96d0.TIF\n",
      "green: MFGTMP_191023110002_C01f96d1.TIF\n",
      "red: MFGTMP_191023110002_C01f96d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 198 out of 300. ID=191023110002_C01f97\n",
      "blue: MFGTMP_191023110002_C01f97d0.TIF\n",
      "green: MFGTMP_191023110002_C01f97d1.TIF\n",
      "red: MFGTMP_191023110002_C01f97d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=709.24\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 199 out of 300. ID=191023110002_C01f98\n",
      "blue: MFGTMP_191023110002_C01f98d0.TIF\n",
      "green: MFGTMP_191023110002_C01f98d1.TIF\n",
      "red: MFGTMP_191023110002_C01f98d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1811.45\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 200 out of 300. ID=191023110002_C01f99\n",
      "blue: MFGTMP_191023110002_C01f99d0.TIF\n",
      "green: MFGTMP_191023110002_C01f99d1.TIF\n",
      "red: MFGTMP_191023110002_C01f99d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 201 out of 300. ID=191023120002_C01f310\n",
      "blue: MFGTMP_191023120002_C01f310d0.TIF\n",
      "green: MFGTMP_191023120002_C01f310d1.TIF\n",
      "red: MFGTMP_191023120002_C01f310d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=2230.16\n",
      "Image is sharp! 2nd focus check FM2=488.91\n",
      "Processed image saved as merged_191023120002_C01f310.TIF \n",
      "\n",
      "\n",
      "Processing image set 202 out of 300. ID=191023120002_C01f311\n",
      "blue: MFGTMP_191023120002_C01f311d0.TIF\n",
      "green: MFGTMP_191023120002_C01f311d1.TIF\n",
      "red: MFGTMP_191023120002_C01f311d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1575.12\n",
      "Image is sharp! 2nd focus check FM2=254.00\n",
      "Processed image saved as merged_191023120002_C01f311.TIF \n",
      "\n",
      "\n",
      "Processing image set 203 out of 300. ID=191023120002_C01f312\n",
      "blue: MFGTMP_191023120002_C01f312d0.TIF\n",
      "green: MFGTMP_191023120002_C01f312d1.TIF\n",
      "red: MFGTMP_191023120002_C01f312d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=2473.75\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 204 out of 300. ID=191023120002_C01f313\n",
      "blue: MFGTMP_191023120002_C01f313d0.TIF\n",
      "green: MFGTMP_191023120002_C01f313d1.TIF\n",
      "red: MFGTMP_191023120002_C01f313d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=3418.14\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 205 out of 300. ID=191023120002_C01f314\n",
      "blue: MFGTMP_191023120002_C01f314d0.TIF\n",
      "green: MFGTMP_191023120002_C01f314d1.TIF\n",
      "red: MFGTMP_191023120002_C01f314d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=2415.21\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 206 out of 300. ID=191023120002_C01f315\n",
      "blue: MFGTMP_191023120002_C01f315d0.TIF\n",
      "green: MFGTMP_191023120002_C01f315d1.TIF\n",
      "red: MFGTMP_191023120002_C01f315d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 207 out of 300. ID=191023120002_C01f316\n",
      "blue: MFGTMP_191023120002_C01f316d0.TIF\n",
      "green: MFGTMP_191023120002_C01f316d1.TIF\n",
      "red: MFGTMP_191023120002_C01f316d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 208 out of 300. ID=191023120002_C01f317\n",
      "blue: MFGTMP_191023120002_C01f317d0.TIF\n",
      "green: MFGTMP_191023120002_C01f317d1.TIF\n",
      "red: MFGTMP_191023120002_C01f317d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 209 out of 300. ID=191023120002_C01f318\n",
      "blue: MFGTMP_191023120002_C01f318d0.TIF\n",
      "green: MFGTMP_191023120002_C01f318d1.TIF\n",
      "red: MFGTMP_191023120002_C01f318d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 210 out of 300. ID=191023120002_C01f319\n",
      "blue: MFGTMP_191023120002_C01f319d0.TIF\n",
      "green: MFGTMP_191023120002_C01f319d1.TIF\n",
      "red: MFGTMP_191023120002_C01f319d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=596.69\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 211 out of 300. ID=191023120002_C01f320\n",
      "blue: MFGTMP_191023120002_C01f320d0.TIF\n",
      "green: MFGTMP_191023120002_C01f320d1.TIF\n"
     ]
    },
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "red: MFGTMP_191023120002_C01f320d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1531.71\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 212 out of 300. ID=191023120002_C01f321\n",
      "blue: MFGTMP_191023120002_C01f321d0.TIF\n",
      "green: MFGTMP_191023120002_C01f321d1.TIF\n",
      "red: MFGTMP_191023120002_C01f321d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 213 out of 300. ID=191023120002_C01f322\n",
      "blue: MFGTMP_191023120002_C01f322d0.TIF\n",
      "green: MFGTMP_191023120002_C01f322d1.TIF\n",
      "red: MFGTMP_191023120002_C01f322d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=568.53\n",
      "Image is sharp! 2nd focus check FM2=430.86\n",
      "Processed image saved as merged_191023120002_C01f322.TIF \n",
      "\n",
      "\n",
      "Processing image set 214 out of 300. ID=191023120002_C01f323\n",
      "blue: MFGTMP_191023120002_C01f323d0.TIF\n",
      "green: MFGTMP_191023120002_C01f323d1.TIF\n",
      "red: MFGTMP_191023120002_C01f323d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 215 out of 300. ID=191023120002_C01f324\n",
      "blue: MFGTMP_191023120002_C01f324d0.TIF\n",
      "green: MFGTMP_191023120002_C01f324d1.TIF\n",
      "red: MFGTMP_191023120002_C01f324d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1155.73\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 216 out of 300. ID=191023120002_C01f325\n",
      "blue: MFGTMP_191023120002_C01f325d0.TIF\n",
      "green: MFGTMP_191023120002_C01f325d1.TIF\n",
      "red: MFGTMP_191023120002_C01f325d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=2784.49\n",
      "Image is sharp! 2nd focus check FM2=412.01\n",
      "Processed image saved as merged_191023120002_C01f325.TIF \n",
      "\n",
      "\n",
      "Processing image set 217 out of 300. ID=191023120002_C01f326\n",
      "blue: MFGTMP_191023120002_C01f326d0.TIF\n",
      "green: MFGTMP_191023120002_C01f326d1.TIF\n",
      "red: MFGTMP_191023120002_C01f326d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 218 out of 300. ID=191023120002_C01f327\n",
      "blue: MFGTMP_191023120002_C01f327d0.TIF\n",
      "green: MFGTMP_191023120002_C01f327d1.TIF\n",
      "red: MFGTMP_191023120002_C01f327d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=957.54\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 219 out of 300. ID=191023120002_C01f328\n",
      "blue: MFGTMP_191023120002_C01f328d0.TIF\n",
      "green: MFGTMP_191023120002_C01f328d1.TIF\n",
      "red: MFGTMP_191023120002_C01f328d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1574.38\n",
      "Image is sharp! 2nd focus check FM2=255.00\n",
      "Processed image saved as merged_191023120002_C01f328.TIF \n",
      "\n",
      "\n",
      "Processing image set 220 out of 300. ID=191023120002_C01f329\n",
      "blue: MFGTMP_191023120002_C01f329d0.TIF\n",
      "green: MFGTMP_191023120002_C01f329d1.TIF\n",
      "red: MFGTMP_191023120002_C01f329d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 221 out of 300. ID=191023120002_C01f330\n",
      "blue: MFGTMP_191023120002_C01f330d0.TIF\n",
      "green: MFGTMP_191023120002_C01f330d1.TIF\n",
      "red: MFGTMP_191023120002_C01f330d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=548.87\n",
      "Image is sharp! 2nd focus check FM2=401.04\n",
      "Processed image saved as merged_191023120002_C01f330.TIF \n",
      "\n",
      "\n",
      "Processing image set 222 out of 300. ID=191023120002_C01f331\n",
      "blue: MFGTMP_191023120002_C01f331d0.TIF\n",
      "green: MFGTMP_191023120002_C01f331d1.TIF\n",
      "red: MFGTMP_191023120002_C01f331d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 223 out of 300. ID=191023120002_C01f332\n",
      "blue: MFGTMP_191023120002_C01f332d0.TIF\n",
      "green: MFGTMP_191023120002_C01f332d1.TIF\n",
      "red: MFGTMP_191023120002_C01f332d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 224 out of 300. ID=191023120002_C01f333\n",
      "blue: MFGTMP_191023120002_C01f333d0.TIF\n",
      "green: MFGTMP_191023120002_C01f333d1.TIF\n",
      "red: MFGTMP_191023120002_C01f333d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 225 out of 300. ID=191023120002_C01f334\n",
      "blue: MFGTMP_191023120002_C01f334d0.TIF\n",
      "green: MFGTMP_191023120002_C01f334d1.TIF\n",
      "red: MFGTMP_191023120002_C01f334d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=538.64\n",
      "Image is sharp! 2nd focus check FM2=333.50\n",
      "Processed image saved as merged_191023120002_C01f334.TIF \n",
      "\n",
      "\n",
      "Processing image set 226 out of 300. ID=191023120002_C01f335\n",
      "blue: MFGTMP_191023120002_C01f335d0.TIF\n",
      "green: MFGTMP_191023120002_C01f335d1.TIF\n",
      "red: MFGTMP_191023120002_C01f335d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 227 out of 300. ID=191023120002_C01f336\n",
      "blue: MFGTMP_191023120002_C01f336d0.TIF\n",
      "green: MFGTMP_191023120002_C01f336d1.TIF\n",
      "red: MFGTMP_191023120002_C01f336d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1085.16\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 228 out of 300. ID=191023120002_C01f337\n",
      "blue: MFGTMP_191023120002_C01f337d0.TIF\n",
      "green: MFGTMP_191023120002_C01f337d1.TIF\n",
      "red: MFGTMP_191023120002_C01f337d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1864.78\n",
      "Image is sharp! 2nd focus check FM2=312.67\n",
      "Processed image saved as merged_191023120002_C01f337.TIF \n",
      "\n",
      "\n",
      "Processing image set 229 out of 300. ID=191023120002_C01f338\n",
      "blue: MFGTMP_191023120002_C01f338d0.TIF\n",
      "green: MFGTMP_191023120002_C01f338d1.TIF\n",
      "red: MFGTMP_191023120002_C01f338d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 230 out of 300. ID=191023120002_C01f339\n",
      "blue: MFGTMP_191023120002_C01f339d0.TIF\n",
      "green: MFGTMP_191023120002_C01f339d1.TIF\n",
      "red: MFGTMP_191023120002_C01f339d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 231 out of 300. ID=191023120002_C01f340\n",
      "blue: MFGTMP_191023120002_C01f340d0.TIF\n",
      "green: MFGTMP_191023120002_C01f340d1.TIF\n",
      "red: MFGTMP_191023120002_C01f340d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 232 out of 300. ID=191023120002_C01f341\n",
      "blue: MFGTMP_191023120002_C01f341d0.TIF\n",
      "green: MFGTMP_191023120002_C01f341d1.TIF\n",
      "red: MFGTMP_191023120002_C01f341d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 233 out of 300. ID=191023120002_C01f342\n",
      "blue: MFGTMP_191023120002_C01f342d0.TIF\n",
      "green: MFGTMP_191023120002_C01f342d1.TIF\n",
      "red: MFGTMP_191023120002_C01f342d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 234 out of 300. ID=191023120002_C01f343\n",
      "blue: MFGTMP_191023120002_C01f343d0.TIF\n",
      "green: MFGTMP_191023120002_C01f343d1.TIF\n",
      "red: MFGTMP_191023120002_C01f343d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 235 out of 300. ID=191023120002_C01f344\n",
      "blue: MFGTMP_191023120002_C01f344d0.TIF\n",
      "green: MFGTMP_191023120002_C01f344d1.TIF\n",
      "red: MFGTMP_191023120002_C01f344d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 236 out of 300. ID=191023120002_C01f345\n",
      "blue: MFGTMP_191023120002_C01f345d0.TIF\n",
      "green: MFGTMP_191023120002_C01f345d1.TIF\n",
      "red: MFGTMP_191023120002_C01f345d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 237 out of 300. ID=191023120002_C01f346\n",
      "blue: MFGTMP_191023120002_C01f346d0.TIF\n",
      "green: MFGTMP_191023120002_C01f346d1.TIF\n",
      "red: MFGTMP_191023120002_C01f346d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1714.59\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 238 out of 300. ID=191023120002_C01f347\n",
      "blue: MFGTMP_191023120002_C01f347d0.TIF\n",
      "green: MFGTMP_191023120002_C01f347d1.TIF\n",
      "red: MFGTMP_191023120002_C01f347d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 239 out of 300. ID=191023120002_C01f348\n",
      "blue: MFGTMP_191023120002_C01f348d0.TIF\n",
      "green: MFGTMP_191023120002_C01f348d1.TIF\n",
      "red: MFGTMP_191023120002_C01f348d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=944.95\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 240 out of 300. ID=191023120002_C01f349\n",
      "blue: MFGTMP_191023120002_C01f349d0.TIF\n",
      "green: MFGTMP_191023120002_C01f349d1.TIF\n",
      "red: MFGTMP_191023120002_C01f349d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1061.04\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 241 out of 300. ID=191023120002_C01f350\n",
      "blue: MFGTMP_191023120002_C01f350d0.TIF\n",
      "green: MFGTMP_191023120002_C01f350d1.TIF\n",
      "red: MFGTMP_191023120002_C01f350d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1867.70\n",
      "Image is sharp! 2nd focus check FM2=439.30\n",
      "Processed image saved as merged_191023120002_C01f350.TIF \n",
      "\n",
      "\n",
      "Processing image set 242 out of 300. ID=191023120002_C01f351\n",
      "blue: MFGTMP_191023120002_C01f351d0.TIF\n",
      "green: MFGTMP_191023120002_C01f351d1.TIF\n",
      "red: MFGTMP_191023120002_C01f351d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1546.35\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 243 out of 300. ID=191023120002_C01f352\n",
      "blue: MFGTMP_191023120002_C01f352d0.TIF\n",
      "green: MFGTMP_191023120002_C01f352d1.TIF\n",
      "red: MFGTMP_191023120002_C01f352d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 244 out of 300. ID=191023120002_C01f353\n",
      "blue: MFGTMP_191023120002_C01f353d0.TIF\n",
      "green: MFGTMP_191023120002_C01f353d1.TIF\n"
     ]
    },
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "red: MFGTMP_191023120002_C01f353d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 245 out of 300. ID=191023120002_C01f354\n",
      "blue: MFGTMP_191023120002_C01f354d0.TIF\n",
      "green: MFGTMP_191023120002_C01f354d1.TIF\n",
      "red: MFGTMP_191023120002_C01f354d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 246 out of 300. ID=191023120002_C01f355\n",
      "blue: MFGTMP_191023120002_C01f355d0.TIF\n",
      "green: MFGTMP_191023120002_C01f355d1.TIF\n",
      "red: MFGTMP_191023120002_C01f355d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 247 out of 300. ID=191023120002_C01f356\n",
      "blue: MFGTMP_191023120002_C01f356d0.TIF\n",
      "green: MFGTMP_191023120002_C01f356d1.TIF\n",
      "red: MFGTMP_191023120002_C01f356d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 248 out of 300. ID=191023120002_C01f357\n",
      "blue: MFGTMP_191023120002_C01f357d0.TIF\n",
      "green: MFGTMP_191023120002_C01f357d1.TIF\n",
      "red: MFGTMP_191023120002_C01f357d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 249 out of 300. ID=191023120002_C01f358\n",
      "blue: MFGTMP_191023120002_C01f358d0.TIF\n",
      "green: MFGTMP_191023120002_C01f358d1.TIF\n",
      "red: MFGTMP_191023120002_C01f358d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 250 out of 300. ID=191023120002_C01f359\n",
      "blue: MFGTMP_191023120002_C01f359d0.TIF\n",
      "green: MFGTMP_191023120002_C01f359d1.TIF\n",
      "red: MFGTMP_191023120002_C01f359d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 251 out of 300. ID=191023120002_C01f360\n",
      "blue: MFGTMP_191023120002_C01f360d0.TIF\n",
      "green: MFGTMP_191023120002_C01f360d1.TIF\n",
      "red: MFGTMP_191023120002_C01f360d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 252 out of 300. ID=191023120002_C01f361\n",
      "blue: MFGTMP_191023120002_C01f361d0.TIF\n",
      "green: MFGTMP_191023120002_C01f361d1.TIF\n",
      "red: MFGTMP_191023120002_C01f361d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 253 out of 300. ID=191023120002_C01f362\n",
      "blue: MFGTMP_191023120002_C01f362d0.TIF\n",
      "green: MFGTMP_191023120002_C01f362d1.TIF\n",
      "red: MFGTMP_191023120002_C01f362d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 254 out of 300. ID=191023120002_C01f363\n",
      "blue: MFGTMP_191023120002_C01f363d0.TIF\n",
      "green: MFGTMP_191023120002_C01f363d1.TIF\n",
      "red: MFGTMP_191023120002_C01f363d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 255 out of 300. ID=191023120002_C01f364\n",
      "blue: MFGTMP_191023120002_C01f364d0.TIF\n",
      "green: MFGTMP_191023120002_C01f364d1.TIF\n",
      "red: MFGTMP_191023120002_C01f364d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 256 out of 300. ID=191023120002_C01f365\n",
      "blue: MFGTMP_191023120002_C01f365d0.TIF\n",
      "green: MFGTMP_191023120002_C01f365d1.TIF\n",
      "red: MFGTMP_191023120002_C01f365d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 257 out of 300. ID=191023120002_C01f366\n",
      "blue: MFGTMP_191023120002_C01f366d0.TIF\n",
      "green: MFGTMP_191023120002_C01f366d1.TIF\n",
      "red: MFGTMP_191023120002_C01f366d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 258 out of 300. ID=191023120002_C01f367\n",
      "blue: MFGTMP_191023120002_C01f367d0.TIF\n",
      "green: MFGTMP_191023120002_C01f367d1.TIF\n",
      "red: MFGTMP_191023120002_C01f367d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 259 out of 300. ID=191023120002_C01f368\n",
      "blue: MFGTMP_191023120002_C01f368d0.TIF\n",
      "green: MFGTMP_191023120002_C01f368d1.TIF\n",
      "red: MFGTMP_191023120002_C01f368d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 260 out of 300. ID=191023120002_C01f369\n",
      "blue: MFGTMP_191023120002_C01f369d0.TIF\n",
      "green: MFGTMP_191023120002_C01f369d1.TIF\n",
      "red: MFGTMP_191023120002_C01f369d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 261 out of 300. ID=191023120002_C01f370\n",
      "blue: MFGTMP_191023120002_C01f370d0.TIF\n",
      "green: MFGTMP_191023120002_C01f370d1.TIF\n",
      "red: MFGTMP_191023120002_C01f370d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 262 out of 300. ID=191023120002_C01f371\n",
      "blue: MFGTMP_191023120002_C01f371d0.TIF\n",
      "green: MFGTMP_191023120002_C01f371d1.TIF\n",
      "red: MFGTMP_191023120002_C01f371d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 263 out of 300. ID=191023120002_C01f372\n",
      "blue: MFGTMP_191023120002_C01f372d0.TIF\n",
      "green: MFGTMP_191023120002_C01f372d1.TIF\n",
      "red: MFGTMP_191023120002_C01f372d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 264 out of 300. ID=191023120002_C01f373\n",
      "blue: MFGTMP_191023120002_C01f373d0.TIF\n",
      "green: MFGTMP_191023120002_C01f373d1.TIF\n",
      "red: MFGTMP_191023120002_C01f373d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 265 out of 300. ID=191023120002_C01f374\n",
      "blue: MFGTMP_191023120002_C01f374d0.TIF\n",
      "green: MFGTMP_191023120002_C01f374d1.TIF\n",
      "red: MFGTMP_191023120002_C01f374d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 266 out of 300. ID=191023120002_C01f375\n",
      "blue: MFGTMP_191023120002_C01f375d0.TIF\n",
      "green: MFGTMP_191023120002_C01f375d1.TIF\n",
      "red: MFGTMP_191023120002_C01f375d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=2508.41\n",
      "Image is sharp! 2nd focus check FM2=265.54\n",
      "Processed image saved as merged_191023120002_C01f375.TIF \n",
      "\n",
      "\n",
      "Processing image set 267 out of 300. ID=191023120002_C01f376\n",
      "blue: MFGTMP_191023120002_C01f376d0.TIF\n",
      "green: MFGTMP_191023120002_C01f376d1.TIF\n",
      "red: MFGTMP_191023120002_C01f376d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1183.45\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 268 out of 300. ID=191023120002_C01f377\n",
      "blue: MFGTMP_191023120002_C01f377d0.TIF\n",
      "green: MFGTMP_191023120002_C01f377d1.TIF\n",
      "red: MFGTMP_191023120002_C01f377d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 269 out of 300. ID=191023120002_C01f378\n",
      "blue: MFGTMP_191023120002_C01f378d0.TIF\n",
      "green: MFGTMP_191023120002_C01f378d1.TIF\n",
      "red: MFGTMP_191023120002_C01f378d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 270 out of 300. ID=191023120002_C01f379\n",
      "blue: MFGTMP_191023120002_C01f379d0.TIF\n",
      "green: MFGTMP_191023120002_C01f379d1.TIF\n",
      "red: MFGTMP_191023120002_C01f379d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 271 out of 300. ID=191023120002_C01f380\n",
      "blue: MFGTMP_191023120002_C01f380d0.TIF\n",
      "green: MFGTMP_191023120002_C01f380d1.TIF\n",
      "red: MFGTMP_191023120002_C01f380d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1867.70\n",
      "Image is sharp! 2nd focus check FM2=906.63\n",
      "Processed image saved as merged_191023120002_C01f380.TIF \n",
      "\n",
      "\n",
      "Processing image set 272 out of 300. ID=191023120002_C01f381\n",
      "blue: MFGTMP_191023120002_C01f381d0.TIF\n",
      "green: MFGTMP_191023120002_C01f381d1.TIF\n",
      "red: MFGTMP_191023120002_C01f381d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1661.01\n",
      "Image is sharp! 2nd focus check FM2=357.32\n",
      "Processed image saved as merged_191023120002_C01f381.TIF \n",
      "\n",
      "\n",
      "Processing image set 273 out of 300. ID=191023120002_C01f382\n",
      "blue: MFGTMP_191023120002_C01f382d0.TIF\n",
      "green: MFGTMP_191023120002_C01f382d1.TIF\n",
      "red: MFGTMP_191023120002_C01f382d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1446.57\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 274 out of 300. ID=191023120002_C01f383\n",
      "blue: MFGTMP_191023120002_C01f383d0.TIF\n",
      "green: MFGTMP_191023120002_C01f383d1.TIF\n",
      "red: MFGTMP_191023120002_C01f383d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1545.67\n",
      "Image is sharp! 2nd focus check FM2=233.04\n",
      "Processed image saved as merged_191023120002_C01f383.TIF \n",
      "\n",
      "\n",
      "Processing image set 275 out of 300. ID=191023120002_C01f384\n",
      "blue: MFGTMP_191023120002_C01f384d0.TIF\n",
      "green: MFGTMP_191023120002_C01f384d1.TIF\n",
      "red: MFGTMP_191023120002_C01f384d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 276 out of 300. ID=191023120002_C01f385\n",
      "blue: MFGTMP_191023120002_C01f385d0.TIF\n",
      "green: MFGTMP_191023120002_C01f385d1.TIF\n",
      "red: MFGTMP_191023120002_C01f385d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1850.40\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 277 out of 300. ID=191023120002_C01f386\n",
      "blue: MFGTMP_191023120002_C01f386d0.TIF\n",
      "green: MFGTMP_191023120002_C01f386d1.TIF\n",
      "red: MFGTMP_191023120002_C01f386d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=2553.25\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 278 out of 300. ID=191023120002_C01f387\n",
      "blue: MFGTMP_191023120002_C01f387d0.TIF\n",
      "green: MFGTMP_191023120002_C01f387d1.TIF\n",
      "red: MFGTMP_191023120002_C01f387d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1948.50\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 279 out of 300. ID=191023120002_C01f388\n",
      "blue: MFGTMP_191023120002_C01f388d0.TIF\n",
      "green: MFGTMP_191023120002_C01f388d1.TIF\n",
      "red: MFGTMP_191023120002_C01f388d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 280 out of 300. ID=191023120002_C01f389\n",
      "blue: MFGTMP_191023120002_C01f389d0.TIF\n",
      "green: MFGTMP_191023120002_C01f389d1.TIF\n"
     ]
    },
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "red: MFGTMP_191023120002_C01f389d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=715.87\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 281 out of 300. ID=191023120002_C01f390\n",
      "blue: MFGTMP_191023120002_C01f390d0.TIF\n",
      "green: MFGTMP_191023120002_C01f390d1.TIF\n",
      "red: MFGTMP_191023120002_C01f390d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 282 out of 300. ID=191023120002_C01f391\n",
      "blue: MFGTMP_191023120002_C01f391d0.TIF\n",
      "green: MFGTMP_191023120002_C01f391d1.TIF\n",
      "red: MFGTMP_191023120002_C01f391d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 283 out of 300. ID=191023120002_C01f392\n",
      "blue: MFGTMP_191023120002_C01f392d0.TIF\n",
      "green: MFGTMP_191023120002_C01f392d1.TIF\n",
      "red: MFGTMP_191023120002_C01f392d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 284 out of 300. ID=191023120002_C01f393\n",
      "blue: MFGTMP_191023120002_C01f393d0.TIF\n",
      "green: MFGTMP_191023120002_C01f393d1.TIF\n",
      "red: MFGTMP_191023120002_C01f393d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 285 out of 300. ID=191023120002_C01f394\n",
      "blue: MFGTMP_191023120002_C01f394d0.TIF\n",
      "green: MFGTMP_191023120002_C01f394d1.TIF\n",
      "red: MFGTMP_191023120002_C01f394d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 286 out of 300. ID=191023120002_C01f395\n",
      "blue: MFGTMP_191023120002_C01f395d0.TIF\n",
      "green: MFGTMP_191023120002_C01f395d1.TIF\n",
      "red: MFGTMP_191023120002_C01f395d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 287 out of 300. ID=191023120002_C01f396\n",
      "blue: MFGTMP_191023120002_C01f396d0.TIF\n",
      "green: MFGTMP_191023120002_C01f396d1.TIF\n",
      "red: MFGTMP_191023120002_C01f396d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=559.79\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 288 out of 300. ID=191023120002_C01f397\n",
      "blue: MFGTMP_191023120002_C01f397d0.TIF\n",
      "green: MFGTMP_191023120002_C01f397d1.TIF\n",
      "red: MFGTMP_191023120002_C01f397d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 289 out of 300. ID=191023120002_C01f398\n",
      "blue: MFGTMP_191023120002_C01f398d0.TIF\n",
      "green: MFGTMP_191023120002_C01f398d1.TIF\n",
      "red: MFGTMP_191023120002_C01f398d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 290 out of 300. ID=191023120002_C01f399\n",
      "blue: MFGTMP_191023120002_C01f399d0.TIF\n",
      "green: MFGTMP_191023120002_C01f399d1.TIF\n",
      "red: MFGTMP_191023120002_C01f399d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 291 out of 300. ID=191023120002_C01f400\n",
      "blue: MFGTMP_191023120002_C01f400d0.TIF\n",
      "green: MFGTMP_191023120002_C01f400d1.TIF\n",
      "red: MFGTMP_191023120002_C01f400d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=703.84\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 292 out of 300. ID=191023120002_C01f401\n",
      "blue: MFGTMP_191023120002_C01f401d0.TIF\n",
      "green: MFGTMP_191023120002_C01f401d1.TIF\n",
      "red: MFGTMP_191023120002_C01f401d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=976.95\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 293 out of 300. ID=191023120002_C01f402\n",
      "blue: MFGTMP_191023120002_C01f402d0.TIF\n",
      "green: MFGTMP_191023120002_C01f402d1.TIF\n",
      "red: MFGTMP_191023120002_C01f402d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 294 out of 300. ID=191023120002_C01f403\n",
      "blue: MFGTMP_191023120002_C01f403d0.TIF\n",
      "green: MFGTMP_191023120002_C01f403d1.TIF\n",
      "red: MFGTMP_191023120002_C01f403d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=859.74\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 295 out of 300. ID=191023120002_C01f404\n",
      "blue: MFGTMP_191023120002_C01f404d0.TIF\n",
      "green: MFGTMP_191023120002_C01f404d1.TIF\n",
      "red: MFGTMP_191023120002_C01f404d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 296 out of 300. ID=191023120002_C01f405\n",
      "blue: MFGTMP_191023120002_C01f405d0.TIF\n",
      "green: MFGTMP_191023120002_C01f405d1.TIF\n",
      "red: MFGTMP_191023120002_C01f405d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=1567.56\n",
      "Image is sharp! 2nd focus check FM2=265.17\n",
      "Processed image saved as merged_191023120002_C01f405.TIF \n",
      "\n",
      "\n",
      "Processing image set 297 out of 300. ID=191023120002_C01f406\n",
      "blue: MFGTMP_191023120002_C01f406d0.TIF\n",
      "green: MFGTMP_191023120002_C01f406d1.TIF\n",
      "red: MFGTMP_191023120002_C01f406d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 298 out of 300. ID=191023120002_C01f407\n",
      "blue: MFGTMP_191023120002_C01f407d0.TIF\n",
      "green: MFGTMP_191023120002_C01f407d1.TIF\n",
      "red: MFGTMP_191023120002_C01f407d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 299 out of 300. ID=191023120002_C01f408\n",
      "blue: MFGTMP_191023120002_C01f408d0.TIF\n",
      "green: MFGTMP_191023120002_C01f408d1.TIF\n",
      "red: MFGTMP_191023120002_C01f408d2.TIF\n",
      "Image might be sharp... 1st focus check FM1=615.04\n",
      "Image is actually blurry; removed. \n",
      "\n",
      "\n",
      "Processing image set 300 out of 300. ID=191023120002_C01f409\n",
      "blue: MFGTMP_191023120002_C01f409d0.TIF\n",
      "green: MFGTMP_191023120002_C01f409d1.TIF\n",
      "red: MFGTMP_191023120002_C01f409d2.TIF\n",
      "Image is blurry; removed. \n",
      "\n",
      "All done!\n"
     ]
    }
   ],
   "source": [
    "for n, x in enumerate(ID_list):\n",
    "    blue = None\n",
    "    green = None\n",
    "    red = None\n",
    "    print()\n",
    "    print(f'Processing image set {n+1} out of {len(ID_list)}. ID={x}')\n",
    "\n",
    "    # load each channel\n",
    "    for entry in os.scandir(folder_path): # import each channel\n",
    "        if x in entry.name and entry.name.endswith('d0.TIF'): # change the suffix of your image files to match\n",
    "            blue = cv2.imread(entry.path, 0)\n",
    "            print(f\"blue: {entry.name}\")\n",
    "        if x in entry.name and entry.name.endswith('d1.TIF'):\n",
    "            green = cv2.imread(entry.path, 0)\n",
    "            print(f\"green: {entry.name}\")\n",
    "        if x in entry.name and entry.name.endswith('d2.TIF'):\n",
    "            red = cv2.imread(entry.path, 0)\n",
    "            print(f\"red: {entry.name}\")\n",
    "    # check if all channels are correctly loaded\n",
    "    if blue is None:\n",
    "        print(\"No blue channel found. Skip...\")\n",
    "        continue\n",
    "    elif green is None:\n",
    "        print(\"No green channel found. Skip...\")\n",
    "        continue\n",
    "    elif red is None:\n",
    "        print(\"No red channel found. Skip...\")\n",
    "        continue\n",
    "    \n",
    "    # use DAPI (blue channel) to do the 1st rnd of blurry removal\n",
    "    edgesBlue = cv2.Canny(blue, 100, 200)\n",
    "    fmBlue = variance_of_laplacian(edgesBlue)\n",
    "    \n",
    "    if fmBlue > 500: # focus threshold, higher = sharper\n",
    "        print(f'Image might be sharp... 1st focus check FM1={fmBlue:.2f}')\n",
    "        # blur out over-exposed cells\n",
    "        mask = find_blobs(red)\n",
    "        blrred = blur_blobs(red, mask)\n",
    "                \n",
    "        # remove air bubbles\n",
    "        kernel = cv2.getStructuringElement(cv2.MORPH_RECT,(5,5))  # taking a matrix of size 5 as kernel\n",
    "        morph = cv2.morphologyEx(blrred, cv2.MORPH_OPEN, kernel) # (erosion followed by dilation)\n",
    "            \n",
    "        # use TRITC (red channel) to do the 2nd rnd of blurry removal\n",
    "        edgesRed = cv2.Canny(morph, 50, 200)\n",
    "        fmRed = variance_of_laplacian(edgesRed)\n",
    "            \n",
    "        if fmRed > 200: # focus threshold, higher = sharper\n",
    "            print(f'Image is sharp! 2nd focus check FM2={fmRed:.2f}')\n",
    "            # uncomment to perform illumination correction\n",
    "            #blue = illum_corr(blue)\n",
    "            green = hist_adj(green, clahe=False, offset=0)\n",
    "            red = hist_adj(red, clahe=False, offset=0)\n",
    "                \n",
    "            mergedImg = cv2.merge([blue, green, red])\n",
    "            fName = 'merged_' + str(x) + '.TIF'\n",
    "            print(f\"Processed image saved as {fName}\", '\\n')\n",
    "            cv2.imwrite(f\"{dst_folder}\"+fName, mergedImg)\n",
    "        else:\n",
    "            print(\"Image is actually blurry; removed.\", '\\n')    \n",
    "    else:\n",
    "        print(\"Image is blurry; removed.\", '\\n')\n",
    "print(\"All done!\")"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": []
  }
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