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Updated Reference Genome Sequence and Annotation of Mycobacterium bovis AF2122/97

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Updated Reference Genome Sequence and Annotation of Mycobacterium bovis AF2122/97

PMID: 28385856 PMCID: PMC5383904 DOI: 10.1128/genomeA.00157-17

Kerri M. Malone(a), Damien Farrell(a), Tod P. Stuber(b), Olga T. Schubert(c), Ruedi Aebersold(c), Suelee Robbe-Austerman(b), Stephen V. Gordon(a)(d)(e)(f)

School of Veterinary Medicine, University College Dublin, Dublin, Ireland(a); Diagnostic Bacteriology Laboratory,National Veterinary Services Laboratories, Ames, Iowa, USA(b); Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland(c); School of Medicine, University College Dublin, Dublin,Ireland(d); School of Biomolecular and Biomedical Science, University College Dublin, Dublin, Ireland(e); UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin, Ireland(f)

ABSTRACT

We report here an update to the reference genome sequence of the bovine tuberculosis bacillus Mycobacterium bovis AF2122/97, generated using an integrative multiomics approach. The update includes 42 new coding sequences (CDSs), 14 modified annotations, 26 single-nucleotide polymorphism (SNP) corrections, and disclosure that the RD900 locus, previously described as absent from the genome, is in fact present.