Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Testing #37

Merged
merged 4 commits into from
Feb 29, 2024
Merged
Show file tree
Hide file tree
Changes from all commits
Commits
File filter

Filter by extension

Filter by extension

Conversations
Failed to load comments.
Loading
Jump to
Jump to file
Failed to load files.
Loading
Diff view
Diff view
1 change: 1 addition & 0 deletions CHANGELOG.md
Original file line number Diff line number Diff line change
Expand Up @@ -4,6 +4,7 @@ CHANGELOG
#### Version 1.0.9 (2024.02.29)
* Improving variable and file names
* Correcting output plot specifications
* Increase logger use, improve logger format

#### Version 1.0.8 (2024.02.28)
* Handling of GenBank features with multiple qualifiers of the same name
Expand Down
2 changes: 1 addition & 1 deletion DESCRIPTION
Original file line number Diff line number Diff line change
@@ -1,6 +1,6 @@
Package: PACVr
Version: 1.0.9
Date: 2024-02-28
Date: 2024-02-29
Title: Plastome Assembly Coverage Visualization
Authors@R: c(person("Gregory", "Smith", role=c("ctb")),
person("Nils", "Jenke", role=c("ctb")),
Expand Down
11 changes: 5 additions & 6 deletions R/PACVr.R
Original file line number Diff line number Diff line change
Expand Up @@ -83,7 +83,7 @@ PACVr.vizWithRCircos <- function(gbkData,
#' value of the average coverage instead of an absolute value
#' @param textSize a numeric value that specifies the relative font size of the
#' text element in the visualization
#' @param verbose a boolean, that when TRUE, generates additional files with
#' @param tabularCovStats a boolean, that when TRUE, generates additional files with
#' detailed genomic region information
#' @param output a character string that specifies the name of, and path to,
#' the output file
Expand All @@ -98,15 +98,15 @@ PACVr.vizWithRCircos <- function(gbkData,
#' outFile <- paste(tempdir(), "/NC_045072__all_reads.pdf", sep="")
#' PACVr.complete(gbkFile=gbkFile, bamFile=bamFile, windowSize=250, logScale=FALSE,
#' threshold=0.5, IRCheck=1, relative=TRUE, textSize=0.5,
#' verbose=FALSE, output=outFile)
#' tabularCovStats=FALSE, output=outFile)
#' }
#' \dontrun{
#' gbkFile <- system.file("extdata", "MG936619/MG936619.gb", package="PACVr")
#' bamFile <- system.file("extdata", "MG936619/MG936619_subsampled.bam", package="PACVr")
#' outFile <- paste(tempdir(), "/MG936619_CoverageViz.pdf", sep="")
#' PACVr.complete(gbkFile=gbkFile, bamFile=bamFile, windowSize=50, logScale=FALSE,
#' threshold=0.5, IRCheck=NA, relative=TRUE, textSize=0.5,
#' verbose=FALSE, output=outFile)
#' tabularCovStats=FALSE, output=outFile)
#' }

PACVr.complete <- function(gbkFile,
Expand All @@ -117,7 +117,7 @@ PACVr.complete <- function(gbkFile,
IRCheck=NA,
relative=TRUE,
textSize=0.5,
verbose=FALSE,
tabularCovStats=FALSE,
output=NA) {
######################################################################
read.gbData <- PACVr.read.gb(gbkFile)
Expand All @@ -131,7 +131,6 @@ PACVr.complete <- function(gbkFile,
logger::log_fatal('Parsing of any sequence features unsuccessful.')
return(-1)
}

###################################
plotSpecs <- getPlotSpecs(logScale,
threshold,
Expand All @@ -145,7 +144,7 @@ PACVr.complete <- function(gbkFile,
plotSpecs$logScale)

###################################
if (verbose) {
if (tabularCovStats) {
PACVr.compileCovStats(gbkData,
coverage$raw,
analysisSpecs,
Expand Down
16 changes: 8 additions & 8 deletions R/RCircosOps.R
Original file line number Diff line number Diff line change
Expand Up @@ -42,19 +42,19 @@ PACVr.Histogram.Plot <- function(hist.data = NULL,
genomic.columns = 3,
is.sorted = TRUE) {
if (is.null(hist.data)) {
warning("Genomic data missing in input.")
stop()
logger::log_error("Genomic data missing in input.")
stop() # Should 'stop()' be replaced with 'return(NULL)' ?
}
boundary <- RCircos::RCircos.Get.Plot.Boundary(track.num, side, inside.pos, outside.pos, FALSE)
outerPos <- boundary[1]
innerPos <- boundary[2]
if (is.null(genomic.columns) || genomic.columns < 2 || genomic.columns > 3) {
warning("Number of columns for genomic position incorrect.")
stop()
logger::log_error("Number of columns for genomic position incorrect.")
stop() # Should 'stop()' be replaced with 'return(NULL)' ?
}
if (is.null(data.col) || data.col <= genomic.columns) {
warning(paste("Number of input columns must be > ", genomic.columns, ".", sep=""))
stop()
logger::log_error(paste("Number of input columns must be > ", genomic.columns, ".", sep=""))
stop() # Should 'stop()' be replaced with 'return(NULL)' ?
}
RCircos.Pos <- RCircos::RCircos.Get.Plot.Positions()
RCircos.Par <- RCircos::RCircos.Get.Plot.Parameters()
Expand All @@ -72,8 +72,8 @@ PACVr.Histogram.Plot <- function(hist.data = NULL,
min.value <- min(histValues)
} else {
if (min.value > max.value) {
warning("min.value must be greater than max.value.")
stop()
logger::log_error("min.value must be greater than max.value.")
stop() # Should 'stop()' be replaced with 'return(NULL)' ?
}
}
histHeight <- RCircos::RCircos.Get.Data.Point.Height(histValues,
Expand Down
4 changes: 2 additions & 2 deletions R/coverageCalcOps.R
Original file line number Diff line number Diff line change
Expand Up @@ -13,8 +13,8 @@ CovCalc <- function(coverageRaw,
# RETURNS:
# data.frame with region names, chromosome start, chromosome end and coverage calcucation
if (!is.numeric(windowSize) | windowSize < 0) {
warning("User-selected window size must be >= 1.")
stop()
logger::log_error("User-selected window size must be >= 1.")
stop() # Should 'stop()' be replaced with 'return(NULL)' ?
}
bins <- GenomicRanges::tileGenome(
sum(IRanges::runLength(coverageRaw)),
Expand Down
4 changes: 2 additions & 2 deletions R/helpers.R
Original file line number Diff line number Diff line change
Expand Up @@ -12,8 +12,8 @@ HistCol <- function(cov, threshold, relative, logScale) {
# color vector
# Error handling
if (!is.numeric(threshold) | threshold < 0) {
warning("User-defined coverage depth threshold must be >=1.")
stop()
logger::log_error("User-defined coverage depth threshold must be >=1.")
stop() # Should 'stop()' be replaced with 'return(NULL)' ?
}
if (relative == TRUE & logScale) {
threshold <- mean(cov[, 4]) + log(threshold)
Expand Down
8 changes: 3 additions & 5 deletions R/visualisationOps.R
Original file line number Diff line number Diff line change
Expand Up @@ -216,15 +216,13 @@ createVizFile <- function(plotSpecs) {
if (outputType == "pdf") {
pdf(output,
width=10,
height=10,
units="cm",
res=600)
height=10)
} else if (outputType == "png") {
png(output,
width=10,
height=10,
units="cm",
res=600)
units="in",
res=300)
}
}

Expand Down
8 changes: 4 additions & 4 deletions inst/extdata/PACVr_Rscript.R
Original file line number Diff line number Diff line change
Expand Up @@ -44,7 +44,7 @@ CmdLineArgs <- function() {
dest = "threshold",
help = "a numeric value that specifies the threshold for plotting coverage depth bars in red as opposed to the default black [default = %default]",
metavar = "integer"),
make_option(opt_str = c("-irc","--IRCheck"),
make_option(opt_str = c("-i","--IRCheck"),
type = "numeric",
default = 1,
dest = "IRCheck",
Expand All @@ -69,10 +69,10 @@ CmdLineArgs <- function() {
dest = "textSize",
help = "a numeric value that specifies the relative font size of the text element in the visualization [default = %default]",
metavar = "integer"),
make_option(opt_str = c("-v","--verbose"),
make_option(opt_str = c("-c","--tabularCovStats"),
type = "logical",
default = FALSE,
dest = "verbose",
dest = "tabularCovStats",
help = paste("a boolean, that when TRUE, generates additional files with",
"detailed genomic region information"),
metavar = "logical"),
Expand Down Expand Up @@ -111,7 +111,7 @@ PACVr.complete(gbkFile = opt$gbkFile,
IRCheck = opt$IRCheck,
relative = opt$relative,
textSize = opt$textSize,
verbose = opt$verbose,
tabularCovStats = opt$tabularCovStats,
output = opt$output)

########################################################################
Expand Down
26 changes: 13 additions & 13 deletions inst/extdata/PACVr_run_parallel.R
Original file line number Diff line number Diff line change
Expand Up @@ -25,29 +25,29 @@ run_PACVr <- function(f) {
gbkFile <- paste0(inFileDir, basename(f))
bamFile <- paste0(inFileDir, accNum, "_mapping_OneMoreLocations.sorted.bam")

tmpName <- paste0(inFileDir, accNum, "_CoverageViz_IRCheckFALSE")
#sink(paste0(tmpName, ".log"))
outFile <- paste0(tmpName, ".png")
PACVr.complete(gbkFile, bamFile, windowSize=250, logScale=FALSE,
threshold=0.5, relative=TRUE, textSize=0.5,
output=outFile)
#sink()
# tmpName <- paste0(inFileDir, accNum, "_CoverageViz_IRCheckFALSE")
# outFile <- paste0(tmpName, ".png")
# PACVr.complete(gbkFile, bamFile, windowSize=250, logScale=FALSE,
# threshold=0.5, relative=TRUE, textSize=0.5,
# output=outFile)

# tmpName <- paste0(inFileDir, accNum, "_CoverageViz_IRCheck0")
# sink(paste0(tmpName, ".log"))
# outFile <- paste0(tmpName, ".png")
# PACVr.complete(gbkFile, bamFile, windowSize=250, logScale=FALSE,
# threshold=0.5, relative=TRUE, textSize=0.5,
# IRCheck=0, output=outFile)
# sink()

# tmpName <- paste0(inFileDir, accNum, "_CoverageViz_IRCheck1")
# sink(paste0(tmpName, ".log"))
tmpName <- paste0(inFileDir, accNum, "_CoverageViz_IRCheck1")
outFile <- paste0(tmpName, ".png")
PACVr.complete(gbkFile, bamFile, windowSize=250, logScale=FALSE,
threshold=0.5, relative=TRUE, textSize=0.5,
IRCheck=1, output=outFile)

# tmpName <- paste0(inFileDir, accNum, "_CoverageViz_IRCheck1_withStats")
# outFile <- paste0(tmpName, ".png")
# PACVr.complete(gbkFile, bamFile, windowSize=250, logScale=FALSE,
# threshold=0.5, relative=TRUE, textSize=0.5,
# IRCheck=1, output=outFile)
# sink()
# IRCheck=1, tabularCovStats=TRUE, output=outFile)
}

foreach(i=1:length(inFiles), .packages=c("PACVr")) %dopar% {
Expand Down
8 changes: 4 additions & 4 deletions man/PACVr.complete.Rd

Some generated files are not rendered by default. Learn more about how customized files appear on GitHub.

2 changes: 1 addition & 1 deletion vignettes/PACVr_Vignette.Rnw
Original file line number Diff line number Diff line change
Expand Up @@ -72,7 +72,7 @@ outFile <- paste(tempdir(), "/NC_045072_CoverageViz.pdf", sep="")
PACVr.complete(gbkFile, bamFile, windowSize=250,
logScale=FALSE, threshold=0.5, syntenyLineType=1,
relative=TRUE, textSize=0.5, regionsCheck=FALSE,
verbose=FALSE, output=outFile)
tabularCovStats=FALSE, output=outFile)
\end{BGVerbatim}

\subsection{Via Unix shell}
Expand Down