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veloPiPe

Universal count pipeline for RNA velocity estimation. VeloPiPe potentially can be applied to any scRNA-seq protocol including MARS-seq, SMART-seq, Dropt-seq, 10x Genomic, etc. as it only needs bam files containing final alignments. The pipline outputs spliced/unspliced matrices in Matrix Market and loom formats which are used as an input to RNA velocity estimation tools like scVelo and velocyto.

Anterior Primitive Streak cells