From 9e3d0afb16836a17f4b72bdbcb914509320df8de Mon Sep 17 00:00:00 2001 From: JP Cartailler <5258731+jpcartailler@users.noreply.github.com> Date: Tue, 27 Feb 2024 13:13:12 -0600 Subject: [PATCH 1/2] Updated CHANGELOG.md as per instructions --- CHANGELOG.md | 11 +++++++++++ 1 file changed, 11 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 503590cb..efe81e11 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,6 +3,17 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). + +## [3.2.3] - 2024-XX-XX + +### Enhancements + +- ... + +### Fixes + +- Fixed output documentation where sections 5 and 6 of output docs were hidden + ## [3.2.2] - 2024-02-01 ### Enhancements From 028f027d85a00b630512ce5f1ff99d0acdfc3b7f Mon Sep 17 00:00:00 2001 From: JP Cartailler <5258731+jpcartailler@users.noreply.github.com> Date: Tue, 27 Feb 2024 13:16:49 -0600 Subject: [PATCH 2/2] Fixed output docs (section 5 and 6 were hidden) --- docs/output.md | 4 +--- 1 file changed, 1 insertion(+), 3 deletions(-) diff --git a/docs/output.md b/docs/output.md index 78f0dcb9..4cf727ef 100644 --- a/docs/output.md +++ b/docs/output.md @@ -263,7 +263,7 @@ High levels of duplication are not necessarily a problem as long as they are con - `.la_dedup.bam`: Coordinate sorted BAM file after Linear Amplification duplicate removal. This is the final post-processed BAM file and so will be saved by default in the results directory. - `.la_dedup.bam.bai`: BAI index file for coordinate sorted BAM file after Linear Amplification duplicate removal. This is the final post-processed BAM index file and so will be saved by default in the results directory. - `.la_dedup_metrics.txt`: Metrics file from custom deduplication based on read 1 5' start location. - + In assays where linear amplification is used, the resulting library may contain reads that share the same start site but have a unique 3' end due to random cut and tagmentation with Tn5-ME-B. In this case, these duplicates should be removed by filtering all read 1's based on their 5' start site and keeping the read aligning with the highest mapping quality. ## 5. Fragment-based QC @@ -345,8 +345,6 @@ MACS2 has its main parameters exposed through the pipeline configuration. The de ### 6.5. Consensus Peaks - - The merge function from [BEDtools](https://github.com/arq5x/bedtools2) is used to merge replicate peaks of the same experimental group to create a consensus peak set. This can then optionally be filtered for consensus peaks contributed to be a threshold number of replicates using `--replicate_threshold`. ## 7. Peak-based QC