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Edit label of OBI:0003660
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sebastianduesing committed Jul 18, 2024
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4 changes: 2 additions & 2 deletions src/ontology/modules/assays.owl
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</owl:someValuesFrom>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000111>in vitro CRISPR screen using single RNA-Seq</obo:IAO_0000111>
<obo:IAO_0000111>in vitro CRISPR screen using single-cell RNA-seq</obo:IAO_0000111>
<obo:IAO_0000115>An in vitro CRISPR screening assay that uses single cell RNA-Seq to detect induced changes in gene expression</obo:IAO_0000115>
<obo:IAO_0000117>Bjoern Peters</obo:IAO_0000117>
<obo:IAO_0000117>Jennifer Jou</obo:IAO_0000117>
<obo:IAO_0000233>https://github.com/obi-ontology/obi/issues/1784</obo:IAO_0000233>
<obo:IAO_0000234>Jennifer Jou</obo:IAO_0000234>
<rdfs:label>in vitro CRISPR screen using single RNA-Seq</rdfs:label>
<rdfs:label>in vitro CRISPR screen using single-cell RNA-seq</rdfs:label>
</owl:Class>


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2 changes: 1 addition & 1 deletion src/ontology/templates/assays.tsv
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Expand Up @@ -657,7 +657,7 @@ OBI:0003601 quartz crystal microbalance assay quartz crystal microbalance assay
OBI:0003604 multiplexed error-robust fluorescence in situ hybridization assay MERFISH A fluorescence in-situ hybridization assay that uses combinatorial FISH labeling with error resistant encoding schemes for highly multiplexed single-molecule imaging. https://en.wikipedia.org/wiki/Fluorescence_in_situ_hybridization#MERFISH Sebastian Duesing Brain Image Library https://github.com/obi-ontology/obi/issues/1724 subclass fluorescence in-situ hybridization assay
OBI:0003605 single molecule fluorescence in situ hybridization smFISH A fluorescence in-situ hybridization assay that detects single RNA molecules in fixed cells, allowing for both the quantification and localization at the single-cell level and single-molecule resolution. https://doi.org/10.21769/BioProtoc.3070 Sebastian Duesing Brain Image Library https://github.com/obi-ontology/obi/issues/1725 subclass fluorescence in-situ hybridization assay
OBI:0003659 in vitro CRISPR screening assay in vitro CRISPR screening assay An assay in which cells expressing a Cas system are infected with a library of guide RNAs with the intent of causing a perturbation at the target of the guide RNAs. Jennifer Jou|Bjoern Peters Jennifer Jou https://github.com/obi-ontology/obi/issues/1784 subclass assay cas mediated mutagenesis assay cell
OBI:0003660 in vitro CRISPR screen using single RNA-Seq in vitro CRISPR screen using single RNA-Seq An in vitro CRISPR screening assay that uses single cell RNA-Seq to detect induced changes in gene expression Jennifer Jou|Bjoern Peters Jennifer Jou https://github.com/obi-ontology/obi/issues/1784 subclass in vitro CRISPR screening assay single-cell RNA sequencing assay
OBI:0003660 in vitro CRISPR screen using single-cell RNA-seq in vitro CRISPR screen using single-cell RNA-seq An in vitro CRISPR screening assay that uses single cell RNA-Seq to detect induced changes in gene expression Jennifer Jou|Bjoern Peters Jennifer Jou https://github.com/obi-ontology/obi/issues/1784 subclass in vitro CRISPR screening assay single-cell RNA sequencing assay
OBI:0003661 in vitro CRISPR perturbation screen using FISH and flow cytometry in vitro CRISPR perturbation screen using FISH and flow cytometry An in vitro CRISPR screening assay that uses single FISH and flow cytometry to detect induced changes in gene expression of the targeted genes Jennifer Jou|Bjoern Peters Jennifer Jou https://github.com/obi-ontology/obi/issues/1784 subclass in vitro CRISPR screening assay flow cytometry assay|fluorescence in-situ hybridization assay
OBI:0302736 comet assay ready for release single cell gel electrophoresis assay|SCGE assay An assay that measures DNA damage (DNA breakage) in eucaryotic cells exposed to a challenge by determining the size and shape of DNA migration by detecting fluorescently labeled DNA from a cell placed in an electric field using gel electrophoresis PMID:7686265 PMID: 18326531.Mutagenesis. 2008 Mar 6.Recommendations for design of the rat comet assay. Philippe Rocca-Serra subclass assay fluorescence microscopy assay cell ('material entity' and ('has role' some 'dye role') and ('bearer of' some fluorescence)) agarose gel information content entity cellular response to DNA damage stimulus
OBI:0302737 PCR-SSCP assay ready for release polymerase chain reaction-single strand conformation polymorphism assay An assay that identifies DNA sequence variation (mutation, deletion, insertions) in target DNA sequences amplified using polymerase chain reaction using gel electrophoresis and denaturating conditions PMID:18219595 PMID: 17334176.Hum Exp Toxicol. 2007 Jan;26(1):9-18.Is there a role for PCR-SSCP among the methods for missense mutation detection of TP53 gene? Philippe Rocca-Serra subclass genotyping assay polymerase chain reaction|material component separation amplified DNA (image and 'genotype information')
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