diff --git a/pdbfixer/tests/test_charge_and_solvate.py b/pdbfixer/tests/test_charge_and_solvate.py
index da64237..8b2697e 100644
--- a/pdbfixer/tests/test_charge_and_solvate.py
+++ b/pdbfixer/tests/test_charge_and_solvate.py
@@ -11,6 +11,7 @@
         ("110D", -5),
         ("1CNR", 0),
         ("1ESD", -21),
+        ("25c8", -2),
     ],
 )
 def test_charge_and_solvate(pdbCode, soluteCharge):