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Merge pull request #2 from pdimens/anchor
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Anchor
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pdimens committed May 27, 2021
2 parents 0d1dc80 + 6c4aef8 commit 3bc16d6
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358 changes: 146 additions & 212 deletions conda_setup.yml

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36 changes: 27 additions & 9 deletions config.yaml
Original file line number Diff line number Diff line change
Expand Up @@ -8,7 +8,7 @@
# ParentCall2 #
#---------------#
# the filtered VCF file with your genotype likelihoods:
vcf: "tons_of_snps.vcf"
vcf: "out.14.missrecode.vcf"

# Instructions to create pedigree file: https://sourceforge.net/p/lep-map3/wiki/software/LepMap3 Home/#parentcall2
# the pedigree file associated with your data
Expand Down Expand Up @@ -39,7 +39,7 @@ informative: "informativeMask=123"
# JoinSingles2ALL #
#-------------------#
# set this to false if you want to skip joining singles (0) to linkage groups
run_joinsingles2all: true
run_joinsingles2all: false

# these are the parameters for JoinSingles2ALL, and are highly data-dependent
# start with lower values for lod_limit and increase as necessary
Expand All @@ -57,43 +57,45 @@ lod_difference: "lodDifference=2"
exp_lg: 24

# Set iterations to the number of iterations you want per chromosome (more is better). Recommend 30 or more
iterations: 5
iterations: 100

# Set threads_per to the number of threads you would like to use per linkage group for ordering
threads_per: 5
threads_per: 2

# Choose your distance method by commenting the line you dont want
dist_method: "useKosambi=1"
#dist_method: "useMorgan=1"

# number of iterations to use of OrderMarkers2 phasing.
# this number is typically 1-2
phase_iterations: 1
phase_iterations: 2

#-----------------#
# Edge Trimming #
#-----------------#
# Edge trimming will examine the first and last X% of markers in a linkage group
# and remove clusters that are N centimorgans away from the next marker
# you can "skip" trimming by making edge_length really low (e.g. 1)
# and trim_cutoff really high (e.g. 50)

# Set edge_length to the percent number of markers you would like to examine from either end of the linkage group
# Value can be an integer or decimal, i.e. 15 is the same as 0.15, which both mean "15%"
edge_length: 15
edge_length: 1

# Set trim_cuttoff to the centiMorgan distance cutoff (10 is reasonable)
trim_cutoff: 10
trim_cutoff: 40



####################
#### Lep-Anchor ####
####################
# change this to true if you also want to run Lep-Anchor
run_lepanchor: false
run_lepanchor: true

# the path to the genome assembly you are trying to anchor
# ideally it *is not* gzipped and ends in .fa, but there are built-in workarounds if it is.
assembly: "boa.constrictor.fasta"
assembly: "YFT.genome.latest.fasta"

# the number of linkage groups you have
lg_count: 24
Expand All @@ -112,3 +114,19 @@ proximity_file: "/dev/null"
OS_info: "Ubuntu"
#OS_info: "CentOS5"
#OS_info: "CentOS6"


#-----------------#
# Edge Trimming #
#-----------------#
# Edge trimming will examine the first and last X% of markers in a linkage group
# and remove clusters that are N% centimorgans (of the total cM span) away from
# the next marker. You can "skip" trimming by making edge_length really low (e.g. 1)
# and trim_cutoff really high (e.g. 50)

# Set edge_length to the percent number of markers you would like to examine from either end of the linkage group
# Value can be an integer or decimal, i.e. 15 is the same as 0.15, which both mean "15%"
LA_edge_length: 20

# Set trim_cuttoff to the centiMorgan distance cutoff (5 is reasonable)
LA_trim_cutoff: 3
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