BarcodeSimilarity.R is a short script to test the similarity of next-generation sequencing barcodes. It is helpful to ensure no barcode collisions will occur.
You need to load a data frame where each row is a barcode sequence of equal length. The script will generate a heatmap where the color indicates the number of differences between molecular barcodes.
Two examples are included:
- In the first, we test the similarity between 8 bp Illumina library barcodes using the script BarcodeSimilarity.R and Illumina_libraries/bcs.txt as input. The output is Illumina_libraries/hamming.png (see below).
- In the second, we test the similarity between barcodes of the SunCatcher lineage tracing system using the similar script McAllister_SunCatcher/McAllister_barcodes.R and McAllister_SunCatcher/BC_sequences.xlsx as input. The output is McAllister_SunCatcher/hamming.pdf.
