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dbdimitrov committed Sep 2, 2024
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4 changes: 2 additions & 2 deletions README.md
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Expand Up @@ -12,9 +12,9 @@ LIANA+ is a scalable framework that integrates and extends existing methods and

<img src="https://raw.githubusercontent.com/saezlab/liana-py/main/docs/source/_static/abstract.png" width="700" align="center">

## Development & Contributions
## Contributions

We welcome suggestions, ideas, and contributions! Please use do not hesitate to contact us, open issues, and check the [contributions guide](https://liana-py.readthedocs.io/en/latest/contributing.html).
We welcome suggestions, ideas, and contributions! Please do not hesitate to contact us, open issues, and check the [contributions guide](https://liana-py.readthedocs.io/en/latest/contributing.html).

## Vignettes
A set of extensive vignettes can be found in the [LIANA+ documentation](https://liana-py.readthedocs.io/en/latest/).
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6 changes: 4 additions & 2 deletions docs/source/notebooks/basic_usage.ipynb
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"\n",
"where we take the minimum probability for each interaction across the score vectors, and we apply a Bonferroni multi-testing correction to the P-values by multiplying them by n.\n",
"\n",
"\n",
"**Notes:**\n",
"\n",
"- Δ Some differences are expected with the original implementations largely due to different preprocessing steps which LIANA+ harmonised across the different methods. Specifically, LIANA+ considers the minimum score (e.g. average expression) for complex subunits, while some methods consider the mean, geometric mean, or simply do not account for complexes at all.\n",
"- † The original [CellChat](https://github.com/jinworks/CellChat) implementation additionally uses information about mediator proteins and pathways, which are specific to the CellChat resource. Since we wish to keep LIANA+ resource-agnostic, **we do not utilise mediator information**, as such while the remainder of the score calculation is identical to CellChat's LR probabilities, some differences are anticipated.\n",
"- † The original [CellChat](https://github.com/jinworks/CellChat) implementation additionally uses information about mediator proteins and pathways, which are specific to the CellChat resource. Since we wish to keep LIANA+ resource-agnostic, **we do not utilise mediator information**, as such while the remainder of the score calculation is identical to CellChat's LR probabilities, some differences are anticipated.\n",
"- Ѫ While we refer to the genes as ligands and receptors for simplicity, these can represent the gene expression also of membrane-bound or extracellular-matrix proteins, as well as heteromeric complexes for which the minimum expression across subunits is used.\n",
"- ‡ 1-vs-rest cell group log2FC for each gene is calculated as $$log2FC = \\log_2\\left(\\text{mean}(X_i)\\right) - \\log_2\\left(\\text{mean}(X_{\\text{not}_i})\\right)$$\n",
"- (*) LIANA considers interactions as occurring only if both the ligand and receptor, as well as all of their subunits, are expressed above a certain proportion of cells in both clusters involved in the interaction (0.1 by default). This can be formulated as an indicator function as follows:\n",
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"metadata": {},
"source": [
"\n",
"### 🙏 <span style=\"color: darkred;\"> Please consider citing the original methods when using their LIANA+ re-implementations in your work! 🙏 </span>\n",
"### 🙏 <span style=\"color: darkred;\"> Please consider citing the original methods when using their LIANA+ adaptations in your work! 🙏 </span>\n",
"\n",
"*While LIANA+ simply aims to ease ligand-receptor inference, the original authors and developers should be credited for their work.*\n",
"\n",
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