Analyzing CODEX data generated from HALO HighPlex FL using Seurat.

[sekekeretsu]

I would like to analyze CODEX data generated from the HALO highplex FL. I know the seurat team has already included a vignette for codex data however this data were generated from Akoya softwares and the format looked different. Currently seurat can load akoya outputs with readakoya()

https://satijalab.org/seurat/articles/seurat5_spatial_vignette_2#human-lymph-node-akoya-codex-system

My input data consisted of basic spread sheet/ table with cells and their intensity values along with my meta data. The issue for me at the moment is, I am finding it hard to convert my spread sheet one that is accepted by seurat that will account for both the gene intensity values and the x, y-coordinates.

Kindly advise how to format the input data to make it compatible with seurat.