Path View Tools

Path Panel Buttons

icon	name	function
	Select Tool	Click on a crossover to add or remove. Click+Drag on a breakpoint to reposition. Shift+Click on one of two adjacent breakpoints to ligate a nick. Alt+Click on a breakpoint to move breakpoint to its boundary. Alt+Shift+Click on Slice Bar to move all breakpoints to their respective boundaries.
	Pencil Tool	Functions like a liberal version of the select tool and also Right Click to once to select start position, then click again to select end position. Confirm in dialog box to install a non-standard crossover.
	Break Tool	Click to install a 3' nick in scaffold or staple.
	Erase Tool	Click to remove continuous stretch of scaffold or staple (up to any breakpoint or crossover).
	Loop Tool	Click on a scaffold strand to install a loop, which causes extra scaffold (and optionally staple) bases to be included at a that position. Click on a staple strand to install a specific sequence. Double-Click with Edit Tool to remove a loop.
	Skip Tool	Click on a scaffold position to install a "skip", which causes scaffold and staple sequence to not be installed at the specific position. Double-Click with Edit Tool to remove a skip.
	Paint Tool	Click on a staple to set to selected color. Use color picker to select specific a color. Use keyboard shortcut "p" to cycle through colors.
	AutoStaple Button	Click to install a default set of staples. It is best to complete the scaffold design before using the AutoStaple button. Note: Staples can be installed manually using the Slice Panel Edit Tool.
	Seq Tool	Click on a scaffold 5' breakpoint to apply a scaffold sequence to a design. Sequences are not currently stored, so this tool must be used whenever an existing design is loaded.
	Frame Button	Click for zoom-to-fit on Path view.