diff --git a/.github/workflows/build.yml b/.github/workflows/build.yml
index ec7904c2e2cc73..a72d4a1bb97cb9 100644
--- a/.github/workflows/build.yml
+++ b/.github/workflows/build.yml
@@ -40,6 +40,50 @@ jobs:
     if: fromJSON(needs.check_source.outputs.run-docs)
     uses: ./.github/workflows/reusable-docs.yml
 
+  check_autoconf_regen:
+    name: 'Check if Autoconf files are up to date'
+    # Don't use ubuntu-latest but a specific version to make the job
+    # reproducible: to get the same tools versions (autoconf, aclocal, ...)
+    runs-on: ubuntu-24.04
+    container:
+      image: ghcr.io/python/autoconf:2024.10.11.11293396815
+    timeout-minutes: 60
+    needs: check_source
+    if: needs.check_source.outputs.run_tests == 'true'
+    steps:
+      - name: Install Git
+        run: |
+          apt install git -yq
+          git config --global --add safe.directory "$GITHUB_WORKSPACE"
+      - uses: actions/checkout@v4
+        with:
+          fetch-depth: 1
+      - name: Runner image version
+        run: echo "IMAGE_VERSION=${ImageVersion}" >> $GITHUB_ENV
+      - name: Check Autoconf and aclocal versions
+        run: |
+          grep "Generated by GNU Autoconf 2.71" configure
+          grep "aclocal 1.16.5" aclocal.m4
+          grep -q "runstatedir" configure
+          grep -q "PKG_PROG_PKG_CONFIG" aclocal.m4
+      - name: Regenerate autoconf files
+        # Same command used by Tools/build/regen-configure.sh ($AUTORECONF)
+        run: autoreconf -ivf -Werror
+      - name: Check for changes
+        run: |
+          git add -u
+          changes=$(git status --porcelain)
+          # Check for changes in regenerated files
+          if test -n "$changes"; then
+            echo "Generated files not up to date."
+            echo "Perhaps you forgot to run make regen-all or build.bat --regen. ;)"
+            echo "configure files must be regenerated with a specific version of autoconf."
+            echo "$changes"
+            echo ""
+            git diff --staged || true
+            exit 1
+          fi
+
   check_generated_files:
     name: 'Check if generated files are up to date'
     # Don't use ubuntu-latest but a specific version to make the job
@@ -69,19 +113,10 @@ jobs:
         uses: hendrikmuhs/ccache-action@v1.2
         with:
           save: false
-      - name: Check Autoconf and aclocal versions
-        run: |
-          grep "Generated by GNU Autoconf 2.71" configure
-          grep "aclocal 1.16.5" aclocal.m4
-          grep -q "runstatedir" configure
-          grep -q "PKG_PROG_PKG_CONFIG" aclocal.m4
       - name: Configure CPython
         run: |
           # Build Python with the libpython dynamic library
           ./configure --config-cache --with-pydebug --enable-shared
-      - name: Regenerate autoconf files
-        # Same command used by Tools/build/regen-configure.sh ($AUTORECONF)
-        run: autoreconf -ivf -Werror
       - name: Build CPython
         run: |
           make -j4 regen-all