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ngs derive
Clay McLeod edited this page Oct 10, 2022
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ngs derive
is the predecessor to the ngsderive
command line tool. The goal of this subcommand is to provide a forensic analysis tool useful in backwards computing information from next-generation sequencing data. The following forensic analysis tools are supported or will be supported in the future:
Forensic Analysis Tool | Description | Implemented |
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ngs derive instrument |
Infer which Illumina instrument was used to generate the data by matching against known instrument and flowcell naming patterns. Each guess comes with a confidence score. | ✅ |
ngs derive strandedness |
Infer from the data whether RNA-Seq data was generated using a Stranded-Forward, Stranded-Reverse, or Unstranded protocol. | ❌ |
ngs derive readlen |
Compute the distribution of read lengths in the file and attempt to guess what the original read length of the experiment was. | ❌ |
ngs derive encoding |
Infers which encoding scheme was used to store PHRED scores as ASCII characters. | ❌ |
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Subcommands
ngs convert
ngs derive
ngs generate
ngs index
ngs list
ngs plot
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ngs qc
- Record-based Facets
- Sequence-based Facets
ngs view
- Development