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LocanDatasets

This repository holds example datasets from super-resolution microscopy.

Available datasets:

  1. Single-molecule localization microscopy data representing nuclear pore complexes.

    The data was generated by dSTORM as described in Löschberger et al.. It shows the gp210 protein of the nuclear pore complex labeled with AlexaFluor647.

    npc_gp210

  2. Single-molecule localization microscopy representing microtubules.

    The data was generated by dSTORM as described in Helmerich et al.. It shows alpha-tubulin as part of microtubules within COS-7 cells. Tubulin was targeted by primary IgG-antibodies labeled with AlexaFluor647 (2.1 degree of labeling) and recorded over 75_000 frames.

    tubulin_cos7

References

(1) Löschberger A, van de Linde S, Dabauvalle MC, Rieger B, Heilemann M, Krohne G, Sauer M., Super-resolution imaging visualizes the eightfold symmetry of gp210 proteins around the nuclear pore complex and resolves the central channel with nanometer resolution. J Cell Sci. 2012, 125:570-5, doi: 10.1242/jcs.098822.

(2) Dominic A. Helmerich, Gerti Beliu, and Markus Sauer, Multiple-Labeled Antibodies Behave Like Single Emitters in Photoswitching Buffer ACS Nano 2020, 14, 10, 12629–12641, DOI: 10.1021/acsnano.0c06099