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Minor grammartical and phrasing fixes
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jfy133 authored and Midnighter committed Jul 2, 2023
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Expand Up @@ -43,8 +43,8 @@ bibliography: paper.bib
Metagenomic analysis is largely concerned with untargeted genetic
characterisation of the taxonomic and functional composition of whole
communities of organisms. Researchers ask questions from metagenomic sequencing
such as 'who is present' (what organisms), and 'what are they doing' (what
functions are they performing)? The nature of this field is such that it
such as 'who is present' (what organisms are present), and 'what are they doing'
(what functions are they performing)? The nature of this field is such that it
intersects with ecology, medicine, statistics, and bioinformatics. Facilitated
by the development of Next-Generation Sequencing (NGS), the field often
generates large datasets consisting of many samples (hundreds) and many
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With TAXPASTA, all of those formats can be converted into a single, standardised
output, that, at a minimum, contains taxonomic identifiers and their relative
abundances as integer counts. It can also be used to aggregate profiles across
sequence abundances as integer counts. It can also be used to aggregate profiles across
samples from the same profiler and merge them into a single, standardised table.
Having a singular format facilitates downstream analyses and comparisons.
TAXPASTA is not the first tool to attempt standardising metagenomic profiles,
Expand Down Expand Up @@ -121,15 +121,15 @@ some of the taxonomic profilers also come with scripts to convert their output
into another format and/or merge multiple profiles into a single table, such as
the Krakentools companion package [@lu_metagenome_2022], these are often focused
on the specific tool or family of tools. Thus, users would have to become
proficient in yet another piece of software per tool or family of tools that
they want to use.
proficient in yet another piece of software per tool or family of tools just for
consistent output files.

[^8]: <https://github.com/bioboxes/rfc/tree/master/data-format>
[^9]: <https://en.wikipedia.org/wiki/Unix_philosophy#Origin>
[^11]: <https://library.qiime2.org/plugins/>

Reading a wide range of formats of primarily shotgun-metagenomic profiling tools
and formats is supported by TAXPASTA, and it is designed to be used as a
TAXPASTA supports reading a wide range of formats of primarily shotgun-metagenomic
profiling tools and formats, and it is designed to be used as a
building block in metagenomic analysis workflows. At the time of writing, it is
able to read profiles from nine different profilers, namely Bracken
[@lu_bracken_2017], Centrifuge [@kim_centrifuge_2016], DIAMOND
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